ABSTRACT K-12 science education is essential for cultivating students’ scientific talents, with teachers playing a critical role in this process. This study investigates the effects of conceptual knowledge and laboratory interventions on pre-service kindergarten teachers’ pedagogical content knowledge (PCK), deriving water as a topic for science activities. Thirty-three pre-service teachers were divided into three groups: Group 1 received lectures on science knowledge, Group 2 participated in lectures supplemented by laboratory experiments, and the third group served as the control group. A mapping approach is utilized to assess the development of PCK among participants. The results revealed that both intervention groups demonstrated significantly improved PCK compared to the control group (p < 0.001). However, no significant difference was observed between the two intervention groups regarding PCK enhancement. Based on these results, we argue the importance of cultivating scientific content knowledge among pre-service education students. Additionally, the science curriculum for these students should differ from common STEM programs, focusing more closely on the scientific knowledge and inquiry skills they will need in their future teaching.
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