Abstract Background: Prior research has indicated that girls have unaddressed fears about breast health and breast cancer. In a Breastcancer.org online survey (SABCS 2008 Abstract#5078), 26% of girls have already worried they may have breast cancer and 73% reported that a relative or close acquaintance had already been diagnosed. Their fears are magnified by the current trend of early onset of breast development and their sensitivity to high media coverage of breast cancer. We hypothesize that girls have limited opportunities and are without the emotional capacity and communication skills necessary to gather and process this information at home, in school, and in the doctor's office.Methods: Breastcancer.org, the Taking Care of Your “Girls” book project, and the Lankenau Hospital Health Education Center conducted an online survey of girls in middle and high schools prior to the delivery of its in-school Basic Breast Health Assembly Program in 13 Philadelphia, Atlanta, Washington, and Los Angeles area schools. 4246 girls attended an assembly, of which 3397 participated in the student survey. 1067 adult female family members (AFFM)—mostly mothers—completed the family member survey.Results: In total, 33% of girls had already learned about breast health and breast cancer in school. 48% of girls wanted to attend the Assembly Program; 93% of AFFMs wanted their girls to participate in the Assembly Program. 50% of girls said they felt uneasy starting the conversation about these topics with their AFFM, 97% of AFFMs said they felt comfortable starting the conversation with their girls, but only 43% of the girls report having the conversation. Of the girls who talked to a parent, 91% talked to their mothers and only 1% talked with their fathers. Girls and AFFMs offered many tips on how to start and sustain a healthy dialogue on these topics: listen, be open, make it fun, pick a private place and time, share experiences, provide facts, and call in experts when you need more information.42%, 18%, and 23% of girls, respectively, have talked to a doctor, sister, or friend about these topics. 50% of girls want to learn breast self-exams from their doctors.Discussion: This survey indicates that girls have unmet informational needs on breast health and breast cancer. This was a source of anxiety for both girls and their AFFMs. There was a willingness to engage in educational activities by both girls and AFFMs, but the dialogue opportunities and communication skills were seen as lacking. Girls identified AFFMs, schools, and doctors as their preferred sources of this information.Girls' ability to express their anxiety, replace fears with facts, learn breast healthy behaviors, and deal with a loved ones' diagnosis requires education, modeling, and an ongoing dialogue. This survey provides guidance for educators and healthcare professionals regarding research into interventions seeking to correct this previously underappreciated gap in public health education. Citation Information: Cancer Res 2009;69(24 Suppl):Abstract nr 1037.