The current study aims to determine whether there is a relationship between transformational leadership and teachers’ motivation and whether this relationship if any, differs significantly based on various demographic variables. Within the framework of this aim, two scales were applied to 418 teachers working at primary, middle, and high schools in Istanbul. The data collection tools used in the study are the “Multidimensional Work Motivational Scale” developed by Gagné et al. (2010), and the “Transformational Leadership Scale” developed by Brestrich (2000). During the analysis of the data collected through scales, mean, frequency, and descriptive values such as standard deviation were used as well as independent groups t-test, multivariate variance analysis (MANOVA), and canonical correlation analysis. The results showed that as teachers perceive their principals as transformational leaders, their motivation including their inner motivation decreases. Besides, teachers’ perceptions of the transformational leadership skills of the principals change according to time of working in their current school, education levels and gender. It was also explored that the motivation status of female teachers was higher than male teachers.