Schinus Molle Research Articles

Entomopathogenic fungi and Schinus molle essential oil: The combination of two eco-friendly agents against Aedes aegypti larvae

Aedes aegypti transmits arbovirus, which is a public health concern. Certain filamentous fungi have the potential to control the disease. Here, the effects of Metarhizium anisopliae s.l. CG 153, Beauveria bassiana s.l. CG 206 and Schinus molle L. were investigated against Aedes aegypti larvae. In addition, the effect of essential oil on fungal development was analyzed. Fungal germination was assessed after combination with essential oil at 0.0025 %, 0.0075 %, 0.005 %, or 0.01 %; all of the oil concentrations affected germination except 0.0025 % (v/v). Larvae were exposed to 0.0025 %, 0.0075 %, 0.005 %, or 0.01 % of the essential oil or Tween 80 at 0.01 %; however, only the essential oil at 0.0025 % achieved similar results as the control. Larvae were exposed to fungi at 107 conidia mL-1 alone or in combination with the essential oil at 0.0025 %. Regardless of the combination, M. anisopliae reduced the median survival time of mosquitoes more than B. bassiana. The cumulative survival of mosquitoes exposed to M. anisopliae alone or in combination with essential oil was 7.5 % and 2 %, respectively, and for B. bassiana, it was 75 % and 71 %, respectively. M. anisopliae + essential oil had a synergistic effect against larvae, whereas B. bassiana + essential oil was antagonistic. Scanning and transmission electron microscopy, and histopathology confirmed that the interaction of M. anisopliae was through the gut and hemocoel. In contrast, the mosquito’s gut was the main route for invasion by B. bassiana. Results from gas chromatography studies demonstrated sabinene and bicyclogermacrene as the main compounds of S. molle, and the in-silico investigation found evidence that both compounds affect a wide range of biological activity. For the first time, we demonstrated the potential of S. molle and its interaction with both fungal strains against A. aegypti larvae. Moreover, for the first time, we reported that S. molle might be responsible for significant changes in larval physiology. This study provides new insights into host-pathogen interplay and contributes to a better understanding of pathogenesis in mosquitoes, which have significant consequences for biological control strategies.

Biological and phytochemical characterization of Phragmanthera regularis (Loranthaceae) from Ethiopia

Phragmanthera regularis is a hemi-parasitic shrub. It is known for treating various health ailments. The aim of this study was to evaluate the antimicrobial activity, toxicity, and chemical characterization of the leaf extracts of P regularis collected from the Schinus molle host plant in Ethiopia. The antimicrobial properties of crude extracts obtained with chloroform, ethyl acetate, methanol, and water solvents were assayed against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. The methanol extract significantly inhibited the growth of S. aureus, E. coli and P. aeruginosa were resistant to any of these solvent extracts. The methanol extract was tested at 175, 550, and 2000 mg/kg body weight doses in white mice and did not reveal any toxicity. The LC-MS qTOF analysis detected flavonoids, phenolic acids, and alkaloids in the crude methanol extract. Further study is needed to investigate the effectiveness of these compounds against S. aureus.

Evaluación de actividad ovicida de extractos vegetales en Tetranychus urticae Koch (Acari: Tetranychidae)

The ovicidal activity of ethanol and hexane extracts of Azadirachta indica A. Juss., Trichilia havanensis Jacq., Roldana ehrenbergiana (Klatt) H. Rob. & Brettell, Argemone mexicana L., Schinus molle L. y Gliricidia sepium (Jacq.) Kunth ex Walp. was evaluated in the laboratory on Tetranychus urticae Koch (Acari: Tetranychidae). The extracts (treatments) were applied by spraying at a concentration of 1,000 mg.L-1 to a known number of eggs (age <18h) on leaf discs of bean (Phaseolus vulgaris L.) (10 leaf discs per treatment), and the percentage of egg mortality was recorded after six days of the treatment. The corrected mortality (percentage) was obtained for each test unit, and a simple ANOVA, followed by multiple comparison of means by the Tukey method (α=0.05), were used to find statistically significant differences between treatment effects. The ethanol and hexane extracts of T. havanensis seeds caused an average corrected egg mortality of 77.7±3.5 and 58.0±3.4 %, respectively, which was significantly higher than the egg mortality caused by the other extracts. The ethanol extracts of the other plant species caused a corrected egg mortality, which ranged from 8.8±9.6 to 42.9±8.3 %, while the corrected mortality of the hexane extracts ranged from 0.2±2.3 to 30.1±4.0 %. The results show that the ethanol extract of T. havanensis seeds has good potential for the development of products with biological activity for the management of T. urticae.

Analysis of Mn accumulation onto plants in urban areas

The biggest problem in dense cities is air pollution and the damage to the environment caused by vehicles. Roadside landscape plants are exposed to pollutants, especially in small urban centers, which are caused by congested traffic. According to the type of landscape plants, being a good biomonitor varies according to their adsorbing capacity of pollutants. Fe and Mn are the leading pollutants originating from emissions. They are selected within the study and have various negative effects on plants in high concentrations. This study aimed to determine the Mn concentration changes according to organ and washing status and location of selected commonly used 14 landscape species in parks. The heavy metal holding capacity of 14 species used was compared the Mn concentration changed significantly depending on the factors evaluated to adsorb them. Chamaecyparis lawsoniana (A. Murr.) Parl. and Photinia serrulata Lindl. Where the highest values are obtained, they can reduce Mn pollution. Schinus molle L. is a suitable type that can monitor and mitigate the Mn concentration in the air.

Insecticidal activity of the essential oil of Schinus areira against Rhipibruchus picturatus (F.) (Coleoptera: Bruchinae), and its inhibitory effects on acetylcholinesterase

During the storage of Prosopis alba pods, substantial quantitative and qualitative losses were observed. One of the main factors is the seed beetle Rhipibruchus picturatus. A key strategy to develop new pest control management is the use of essential oils (EOs) due they are efficient, less toxic, and less persistent in the environment compared to synthetic pesticides. In this context, seeds and leaves of Schinus areira L. (Anacardiaceae) EOs and Citrus spp. EO were studied in the present work. In the leaves of S. areira EO, 1-epi-cadinol, sesquiterpenoid alcohol, was the major compound. On the other hand, the main compounds of the EO extracted from S. areira seeds are the monoterpenes sabinene, and α-pinene. Finally, in the Citrus EO, limonene is the principal component. The three EOs obtained exhibited insecticidal activity against R. picturatus, being the first report of the use of EOs against this insect pest. The best insecticidal results were obtained with the leaves of S. areira EO. Moreover, this EO inhibits the acetylcholinesterase enzyme in vitro assays. Molecular docking studies on acetylcholinesterase (AChE) suggest that the main components of the leaves of S, areira EOs, bind to the active site of the enzyme, in good agreement with in vitro competitive inhibition against AChE observed for this EO. The data obtained demonstrate the potential use of Schinus areira EOs in the development of new storage pest control strategies.

Chemical Composition, Antioxidant, Insecticidal Activity, and Comparative Analysis of Essential Oils of Leaves and Fruits of Schinus molle and Schinus terebinthifolius.

Schinus terebinthifolius Raddi. and Schinus molle L. are perennial woody plants belonging to the Anacardiaceae family, widely distributed in the United States, Europe, Asia, and Africa, and they are broadly used for many applications such as in traditional medicine as an antipyretic, analgesic, depurative, and in the treatment of diseases of the urogenital system as well as culinary and ornamental species. Our work aims to study and compare the chemical composition and the antioxidant and insecticidal activity of the essential oils of the leaves and fruits of the two species of the genus Schinus. The essential oils were characterized by a very spicy aromatic odor, and by the abundance of hydrocarbon monoterpenes in the leaves and fruits of Schinus molle (49.70% and 42.65%), unlike the EOs of the fruits of Schinus terebinthifolius which have a high content in hydrocarbon sesquiterpenes (40.57%). Usually, these oils have shown relatively low antioxidant activity was expressed in IC50; especially, the essential oil of the fruits of S. terebinthifolius revealed a concentration of 3.292 ± 2.82 mg/mL. The evaluation of the insecticidal activity gave good results in the method of exposure of adults of Sitophilus oryzae to EOs by contact; thus, the fruits of Schinus molle are more active against this species of beetle than the other essential oil.

GC-MS Profile, Antioxidant Activity, and In Silico Study of the Essential Oil from Schinus molle L. Leaves in the Presence of Mosquito Juvenile Hormone-Binding Protein (mJHBP) from Aedes aegypti.

Schinus molle is a medicinal plant used as an anti-inflammatory and for rheumatic pain in the traditional medicine of Peru. On the other hand, Aedes aegypti is the main vector of several tropical diseases and the transmitter of yellow fever, chikungunya, malaria, dengue, and Zika virus. In this study, the aim was to investigate the antioxidant activity in vitro and the insecticidal activity in silico, in the presence of the mosquito juvenile hormone-binding protein (mJHBP) from Aedes aegypti, of the essential oil from S. molle leaves. The volatile phytochemicals were analyzed by gas chromatography-mass spectrometry (GC-MS), and the profile antioxidants were examined by DPPH, ABTS, and FRAP assays. The evaluation in silico was carried out on mJHBP (PDB: 5V13) with an insecticidal approach. The results revealed that EO presented as the main volatile components to alpha-phellandrene (32.68%), D-limonene (12.59%), and beta-phellandrene (12.24%). The antioxidant activity showed values for DPPH = 11.42 ± 0.08 μmol ET/g, ABTS = 134.88 ± 4.37 μmol ET/g, and FRAP = 65.16 ± 1.46 μmol ET/g. Regarding the insecticidal approach in silico, alpha-muurolene and gamma-cadinene had the best biding energy on mJHBP (ΔG = −9.7 kcal/mol), followed by beta-cadinene (ΔG = −9.5 kcal/mol). Additionally, the volatile components did not reveal antioxidant activity, and its potential insecticidal effect would be acting on mJHBP from A. aegypti.

Composition of Phenolic Compounds in South African Schinus molle L. Berries.

The Schinus molle tree is notoriously invasive in most parts of the world, and yet as a pseudospice, its berries potentially possess some significant health benefits which need to be explored. Therefore, polar metabolome of seed + husks (SH), husks (H), and de-hulled (DH) berries were profiled and quantified by untargeted metabolomics approach using UPLC-QTOF-MS. A total of 13 gallotannins, three phenolic acids, a phenolic acid glucoside, three phenolic acid esters, an organic acid, a gallotannin derivative, and nine flavonoids were detected and quantified. Phenolic acids ranged between 12.2–295.7; 4.9–77; and 89.7–1613.1 mg/kg in SH, DH seeds and H respectively. Flavonoids ranged between 1.8–267.5; 73.4–80.4; and 124–564.3 mg/kg in SH, DH seeds and H respectively. Gallotannins ranged between 1.1–146.6; 14.8–21.8; and 48.1–664.8 mg/kg in SH, DH seeds and H respectively. Feruloyltartaric A, quercetin 3-O-glucuronide, catechin digalloylshikimic acid B as well as digalloyl quinic acid were some of the dominant secondary metabolites revealed. These results indicate that S. molle berries are a rich source of secondary metabolites with elevated concentrations in the husks, while DH seeds possess lower concentrations to none. These findings open important insights into the potential of S. molle berries as a natural source of antioxidants for the food and pharmaceutical industries.

Efficiency of Schinus molle Essential Oil against Bactericera cockerelli (Hemiptera: Triozidae) and Sitophilus zeamais (Coleoptera: Dryophthoridae)

The present study determines the chemical composition of Schinus molle essential oil and its mortality and repellent effect on Bactericera cockerelli immature stage and Sitophilus zeamais adults. Twenty-four compounds were identified and the most abundant were o-Cymene (29.04), 1R-α-Pinene (15.52), camphene (14.00), and β-myrcene (11.54). On the fifth-instar psyllid nymph, the LC50 and LC90 at 48 h were 442.67 and 864.29 ppm, and for the fourth-instar were 273.41 and 534.67 ppm. The maize-weevil registered an LC50 and LC90 of 343.25 and 986.96 ppm for the fifteenth day. A selection index (Si) of 0.37 with 800 ppm was registered, showing the highest repellent activity, while with the lowest concentration (50 ppm), non-repellent activity was recorded. However, all concentrations above 100 ppm showed repellency against the maize weevil. The study reveals, for the first time, the essential oil’s insecticidal effects on the fourth and fifth nymphal stage of the potato/tomato psyllid B. cockerelli and the usefulness of the essential oil as a repellent against adult of S. zeamais. The Si effect on maize weevil was grouped into categories.

Antifungal properties of hybrid films containing the essential oil of Schinus molle: Protective effect against postharvest rot of tomato

The control of postharvest diseases in tomatoes using hybrid films represents an efficient and inexpensive alternative to avoid economic losses. This work reports on the design, characterization, and in situ application of hybrid films containing chitosan combined with the leaf essential oil of Schinus molle (SmEO) as antifungal agents. These materials were tested in situ on Lycopersicon esculentum cv. uva showing symptoms of soft rot caused by Fusarium oxysporum . According to our analytical conditions, SmEO contained β-phellandrene (15.7%), α-phellandrene (12.1%), elemol (9.1%), apiole (6.4%) and camphene (6.2%) as the most abundant volatiles. Four distinct hybrid films were prepared from 1% chitosan combined with different amounts of SmEO (0.05, 0.1, 0.3 and 0.7% w/v ) to generate four hybrid films named FSm1, FSm2, FSm3 and FSm4. The spectroscopic properties (FT-IR), texture and thickness (SEM), and optical properties (transmittance) of these films revealed that FSm3 and FSm4 had the best physicochemical and in situ antifungal properties to avoid conidial germination and mycelial proliferation of F. oxysporum . The fruits treated with films and essential oil showed a statistically significant delay (>50%) in mycelial growth compared with the control groups (p < 0.05) and a substantial decrease in conidial viability over 6 days (<2%). According to our results, FSm3 and FSm4 significantly avoided the in situ growth of F. oxysporum in tomatoes. Interestingly, all of the films significantly improved fruit firmness in comparison with untreated tomatoes (p < 0.05). The properties of the films reported in this work may improve the shelf life of Lycopersicon esculentum cv. uva. • Films based on chitosan and the essential oil from Schinus molle improved tomato quality. • The physicochemical properties of films suggested their potential use a new fruit barrier. • In situ testes revealed that films delayed the mycelial proliferation of F. oxysporum. • Same tests revealed that films avoided the germination of fungal conidia.

Indigenous knowledge and quantitative ethnobotany of the Tanawal area, Lesser Western Himalayas, Pakistan.

Ethnobotanical field surveys were carried out in the Tanawal area of the Lesser Himalayan Region, Khyber Pakhtunkhawa, Province from April 2016 to October 2017. The area is located between 34.36 (34° 21’ 30 N) latitude and 73.07 (73° 4’ 0 E) longitude with an average elevation of 1374 meters above sea level. Ethnomedicinal data were collected through Participatory Rural Appraisal (PRA), and participants were selected through the snow-boll technique. Semi-structured, in-depth and open-ended interviews were conducted. The data were quantitatively evaluated using ethnomedicinal indices i.e. Relative frequency of citation (RFCs), Fidelity level (FL), and Use Value (UV). The ethnobotanical data were also comparatively analyzed through the Jaccard Index (JI). The study yielded 66 medicinal plants in 62 genera and 43 families. Asteraceae and Solanaceae were the most important families with five medicinal taxa each. Regarding medicinal plant part utilization, leaves (43.28%) were used predominantly, followed by whole plant (14.92%) and fruits (14.92%). Decoction was the main drug formulation applied to 21 species (31.15%) and the oral route was most common (56.1%) while 31.2% of medicinal plants were used for both oral and topical applications. Fifty health disorders were recorded and grouped in 15 categories. Maximum species were used to treat gastrointestinal disorders i.e. 13 species, dermal problems (12 species), and respiratory tract ailments (9). The calculated RFCs ranged between 81 to 31. The most important medicinal plants were Acacia modesta, Citrullus vulgaris, Tamarindus indica, and Momordica charantia with an RGFC of 81 each. The UV ranged between 0.58 and 3.6. Medicinal taxa with the highest UV were Dioscorea deltoidea (3.6), Withania coagulans (3.3), Momordica charantia (3.5), Silybum marianum and Pyrus pashia (3.2). FL values showed that 28 (41.79%) species had a FL value below 50 (74.62%) while 39 (58.20%) had higher FL values. Momordica charantia, Tamarindus indica, Acacia modesta and Citrullus vulgaris were 95.2 each. The Jaccard Index (JI) values ranged from16.77 to 0.98. The current study also reported 16 medicinal plants, commonly used around the globe, have been rarely documented for their medicinal values in the local ethnomedicinal literature i.e. Althaea officinalis, Plantanus orientalis, Jasminum sombac, Maytenus royleana, Cucurbita maxima, Phyllanthus emblica, Citrullus vulgaris. Polygonatum verticilliatum, Caseria tomentosa, Cistanche tubulosa, Bambusa arundinacea, Schinus molle, Tamarindus indica, Pongamia pinnata, Citrus limon and Catharanthus roseus. However, 48 medicinal plants had been reported in the literature but the current study reported their novel medicinal uses. Important taxa should be established in botanical gardens for in-situ conservation, chemical investigation and sustainable utilization. It would also be effective to improve the livelihoods of the local population.

Leaf Fluxes of Carbon Dioxide, Methane and Biogenic Volatile Organic Compounds of the Urban Trees Platanus × acerifolia and Schinus molle in Santiago, Chile

This study assessed leaf fluxes of CO2, CH4 and biogenic volatile organic compounds (BVOC) for two common urban tree species, Platanus × acerifolia (exotic) and Schinus molle (native), widely distributed in Santiago, Chile. The emission factors (EF) and the Photochemical Ozone Creation Index (POCI) for S. molle and P. × acerifolia were estimated. The global EF was 6.4 times higher for P. × acerifolia compared with S. molle, with similar rates of photosynthesis for both species. Isoprene represented more than 86% of the total BVOCs leaf fluxes being 7.6 times greater for P. × acerifolia than S. molle. For P. × acerifolia, BVOCs represented 2% of total carbon fixation while representing 0.24% for S. molle. These results may suggest that plant species growing outside their ecological range may exhibit greater BVOCs leaf fluxes, proportional to photosynthesis, compared to well-adapted ones. The results found may contribute to better urban forest planning.

Investigation of Chemical Composition and Phytotoxic Effects of Essential Oils Obtained from Schinus Molle Leaves and Resins

&#x0D; &#x0D; &#x0D; Chemical composition of the essential oils of Schinus molle leaves and resins, collected from East Mediterranean region of Turkey were identified by gas chromatography/mass spectrometry (GC/MS) and phytotoxic effects of the oils at different concentrations (0.5, 1.0, 2.0, 4.0 and 8.0 μl/ml) were investi- gated on Portulaca oleracea L. and Raphanus raphanistrum subsp. sativus (L.) Domin. At the highest concentration, it was found that the essential oil of S. molle leaves and resins inhibited at rates of 57%, 67% and 70%, 80% the seed germination of purslane and radish, respectively, when compared to the control. Also, S. molle leaves essential oil at the same concentration decreased Radicle length (RL) and Plumule lengths (PL) of purslane and radish in the rates of 82.94, 78.09% and 94.36, 81.30%, while RL and PL of both plants tested were inhibited in the rates of 78.03, 68.99% and 88.91, 76.95% by S. molle resins essential oil. This study is the first report on P. oleracea L. and R. raphanistrum subsp. sativus (L.) Domin about phytotoxic effects of the essential oils of Schinus molle leaves and resins&#x0D; &#x0D; &#x0D;

Eugenia uniflora, Melaleuca armillaris, and Schinus molle essential oils to manage larvae of the filarial vector Culex quinquefasciatus (Diptera: Culicidae).

Populations of Culex quinquefasciatus Say, 1823 (Diptera: Culicidae) have shown resistance to insecticides of the carbamate and organophosphate classes. The objective of this study was to assess the susceptibility of C. quinquefasciatus larvae to essential oils from leaves of Eugenia uniflora L., Melaleuca armillaris (Sol. ex Gaertn.) Sm., and Schinus molle L and C. quinquefasciatus larvae's biochemical responses after their exposure to these leaves. The essential oils were chemically analyzed by GC and GC/MS. First, the lethal concentration for 50% (LC50) values was estimated using different concentrations of essential oils and probit analysis. The larvae were exposed for 1h at the LC50 estimated for each essential oil. The susceptibility of the larvae to essential oils was evaluated using the following biochemical parameters: concentrations of total protein and reduced glutathione; levels of production of hydrogen peroxide and lipid peroxidation; and the activity of the enzyme acetylcholinesterase (AChE). The main chemical constituents in E. uniflora were E-β-ocimene, curzerene, germacrene B, and germacrone; in M. armillaris were 1,8-cineole and terpinolene; and in S. molle were sabinene, myrcene, and sylvestrene. The essential oils had LC50 values between 31.52 and 60.08mg/L, all of which were considered effective. All of them also promoted changes in biochemical parameters when compared to the control treatment. The essential oils of S. molle and E. uniflora inhibited the activity of the AChE enzyme, and the essential oil of M. armillaris increased it. All essential oils had larvicidal activity against C. quinquefasciatus, but the essential oil of E. uniflora was the most efficient. Thus, the findings of the present study suggest that the essential oil of E. uniflora can be considered promising for the development of botanical larvicides.

Hallucinogens, alcohol and shifting leadership strategies in the ancient Peruvian Andes

In the pre-Columbian Andes, the use of hallucinogens during the Formative period (900–300 BC) often supported exclusionary political strategies, whereas, during the Late Horizon (AD 1450–1532), Inca leaders emphasised corporate strategies via the mass consumption of alcohol. Using data from Quilcapampa, the authors argue that a shift occurred during the Middle Horizon (AD 600–1000), when beer made fromSchinus mollewas combined with the hallucinogenAnadenanthera colubrina. The resulting psychotropic experience reinforced the power of the Wari state, and represents an intermediate step between exclusionary and corporate political strategies. This Andean example adds to the global catalogue documenting the close relationship between hallucinogens and social power.

Reactive Oxygen Species-Mediated Cytotoxicity in Liver Carcinoma Cells Induced by Silver Nanoparticles Biosynthesized Using Schinus&nbsp;molle Extract.

Hepatocellular carcinoma (HCC) is the most common primary liver malignancy and is ranked as the third most common cause of cancer-related mortality worldwide. Schinus molle (S. mole) L. is an important medicinal plant that contains many bioactive compounds with pharmacological properties. The role of S. molle leaf extract in the biosynthesis of silver nanoparticles (AgNPs) was determined. The biosynthesized AgNPs were thoroughly characterized by UV–vis spectrophotometry, transmission electron microscopy (TEM), X-ray diffraction (XRD), and dynamic light scattering (DLS) techniques. Furthermore, the cytotoxic effect of the biosynthesized AgNPs using S. molle (SMAgNPs) against HepG2 liver cancer cells was investigated. Reactive oxygen species generation, apoptosis induction, DNA damage, and autophagy activity were analyzed. The results clearly showed that the biosynthesized silver nanoparticles inhibited the proliferation of HepG2 by significantly (p < 0.05) inducing oxidative stress, cytotoxicity, DNA damage, apoptosis, and autophagy in a dose- and time-dependent manner. These findings may encourage integrating the potential of natural products and the efficiency of silver nanoparticles for the fabrication of safe, environmentally friendly, and effective anticancer agents.

Cellulose Fibre from Schinus molle and Its Characterization

The exploitation of biomass represents a major environmental challenge related to the protection of the environment and the progressive exhaust of fossil resources. In this perspective, the main objective of this work is the extraction and the characterization of natural lignocellulosic fibers from the Schinus molle. The cellulose fibre extraction was investigated employing conditions of alkali treatment. After the alkaline steps, a bleaching treatment was done and let to a yield about 45% pure cellulose. The identification of the chemical composition of Schinus molle reveals that this raw material contains a high level of biopolymers with a cellulose rate of 53.2%. Extracted cellulose fibers have been characterized by several techniques such as scanning electron microscopy, Fourier transform infrared, X-ray diffraction, Morfi, and by the determination of their degree of polymerization. FT-IR results confirm the purity of the cellulosic fibers, and XRD analysis reveals that the crystallinity increases after the delignification and bleaching treatments.

Extraction, chemical composition, and antioxidant activity analysis of essential oil from Schinus molle medicinal plant found in Botswana

Extraction, chemical composition, and antioxidant activity analysis of essential oil from Schinus molle medicinal plant found in Botswana

Hostplant and last instar of Amastus walkeri (Rothschild, 1922) from southwestern Peru (Lepidoptera: Erebidae, Arctiinae)

The hostplant, Schinus molle L. (Anacardiaceae) and the last instar of the Arctiinae Amastus walkeri (Rothschild, 1922) are reported for the first time. Several caterpillars with abundant setae and gregarious behavior, were observed feeding on the leaves of Schinus molle L. in the department of Arequipa, southwestern Peru. Ten caterpillars were collected and raised in laboratory condition. Two adults were recovered. According to the external morphology of the adults, they correspond to the species identified as Amastus walkeri (Rothschild).

Schinus molle seedlings production: Influence of slow release and conventional fertilizers

The aim of this study was to evaluate the influence of different formulations of slow-release and conventional fertilizer in Schinus molle seedlings production. The experiment was conducted at São Paulo State University, School of Engineering, municipality of Ilha Solteira, Brazil, in a Pad &amp; Fan greenhouse type (Average Temp. 26 °C and 55% RH). The seedlings were grown in the same cited environment in polyethylene trays filled with commercial substrate. When seedlings presented 3-4 leaves, 30 days after sowing they were transplanted to 4 L plastic bags, with the following treatments: T1 = Control, T2 = Osmocote® 3M (14-14-14), T3 = Basacote® 3M (15-09-12) and T4 = TecNutri® conventional fertilizer (05-13-13). Mixture of soil + commercial substrate (1:1) formed the substrate and fertilizer at a dosage of 3 g L-1 was used. The experimental design was completely randomized with 4 treatments and 06 replications and evaluations were performed 55 days after planting. The following characteristics were analysed: plant height, stem diameter (mm), plant height and stem diameter ratio and number of leaves. For 55 days seedlings the best treatment was the conventional fertilizer TecNutri®