Scanning UV Research Articles

TLC densitometric approach for concurrent determination of quinary mixture for treatment of migraine with appraisal to method greenness and whiteness

A new accurate, precise thin layer chromatographic (TLC densitometric) approach was developed for the simultaneous analysis of ergotamine tartrate (ERG), phenobarbital sodium (PHEN), caffeine anhydrous (CAF), dipyrone sodium (DIP) and meprobamate (MEP) in their pure form and in their combined formulation; (MIGRANIL tablets). TLC separation depended on using a stationary phase of TLC plates F254 (20 × 10 cm) and using a developing system of ethyl acetate: methanol: n- hexane (8:2:3, by volume) with UV scanning at 254 nm for ERG, PHEN, CAF and DIP. For MEP, detection was done at 560 nm after spraying with 0.3% ethanolic solution of para -dimethylaminobenzaldehyde (PDAB) and heating at 110 ºC for 30 min. Analysis was performed in the concentration intervals of 0.06-2, 0.15–5 µg/band for ERG and PHEN, respectively; while for CAF, DIP and MEP it was done in the ranges of 0.3-9, 0.5–10, 1–100 µg/band, respectively. Moreover, the method was used for the quantitation of the five studied drugs in their unique formulation and the recovery% was calculated and found to be 102.04, 100.73, 100.65, 99.91, 1nd 101.77% for ERG, PHEN, CAF, DIP, and MEP, in order and all these outcomes proved excellent selectivity of the method. Other validation parameters were tested following ICH guidelines. Accuracy ranged from 98.02 to 100.53% while the obtained %RSD of interday day precision ranged from 1.215 to 1.940%. The environmental impact and performance of the method was tested with several tools; Analytical eco-scale, green analytical procedure index (GAPI), Analytical GREEnness Metric Approach (AGREE), Blue applicability grade index (BAGI), and RGB additive color model. Results ensured minimum hazardous effect on health and environment. This is the first analytical approach developed for simultaneous analysis of the quinary mixture which can be easily applied in any analytical laboratory.

Effect of indirect electrochemical pretreatment on the anaerobic digestion of swine manure

The increase in pig population has led to an accumulation of swine manure (SM) in the environment. Improper disposal of this waste can lead to environmental pollution. Anaerobic digestion (AD) is a promising method for utilizing SM, but its industrial application is still limited due to high lignocellulose content and a suboptimal carbon-to-nitrogen ratio. In this study, indirect electrochemical pretreatment (IEP) was used to improve AD of SW. IEP involves treating SM with reactive oxygen species generated by tap water electrolysis. Three different pretreatment modes (acidic-alkaline, acidic, and alkaline-acidic-alkaline) were tested, differing in the time of water flow through the electrochemical unit of the IEP device. Using scanning electron microscopy and UV–Vis spectroscopy, it was found that as a result of IEP, the particle size of the SM decreased from 200 to 500 to 15–50 μm. This led to an increase in biodegradability by 37–38%, compared to untreated SM. Using the alkaline-acid-alkaline pretreatment mode, the highest methane yield of 94.48 ± 1.42 mL CH4/g VS was obtained, which was 41.28 ± 2.21% higher than in the control group. During AD of pretreated SM, more complex microbial aggregates were observed in the anaerobic microbial community, and the relative abundance of hydrolytic (Bacillus, Ureibacillus, and Geobacillus genera) and syntrophic (Smithella genus and Christensenellaceae R-7 group) microorganisms increased.

Insights into the role of hexa-bacterial consortium for bioremediation of soil contaminated with chlorantraniliprole

This study evaluates the efficacy of discrete bacterial consortia in bioremediating sandy loamy soil deliberately contaminated with 20 mg/kg of chlorantraniliprole (CAP). It monitors alterations in total bacterial populations and CO2 emissions, tracking residual CAP levels through UV scanning and HPLC analysis. Six active bacterial degraders (four Bacillus strains (B. subtilis subsp. subtilis AZFS3, B. pumilus AZFS5, B. mojavensis AZFS15, and B. paramycoides AZFS18), one Alcaligenes strain (A. aquatilis KZFS11), and one Pseudomonas strain (P. aeruginosa KZFS4)) were used in single or combined preparations and grown on trypticase soy broth for 24 h at 30 °C before preparing the inoculants and adjusting the bacterial cell count to 107 CFU/dwt g soil. The bacterial consortia were added to the CAP-contaminated soil and incubated for 20 days at 30 °C. The di-, tetra-, and hexa-bacterial consortia recorded the highest levels of viable bacteria, reaching their peak after 3 to 11 days of incubation. Then, they declined to the minimum levels at the end of the 20 days, which coincided with their complete removal of CAP from the soil. At the end of the incubation period (20 days), the CAP was mainly biodegraded, scoring biodegradation rates of 90.05%, 93.65%, and 98.65% for T3, T4, and T5, respectively. This concurred with the highest average CO2 production. Based on the results of the HPLC analysis, the hexa-bacterial consortium T5 demonstrated the highest rate of CAP biodegradation (99.33%) after a 20-day incubation period, resulting in the lowest residual level of CAP in the soil (0.67%). Bioinformatic analysis predicted that the CAP biodegradation pathway reached CO2 and H2O. Under optimized conditions, the hexa-bacteria consortium is the most effective CAP biodegraded and is recommended as an eco-friendly treatment for eliminating CAP pollution in the field.

DEVELOPMENT, CHARACTERIZATION, AND OPTIMIZATION OF REPAGLINIDE LOADED SPANLASTICS ALONG WITH INVESTIGATION OF DRUG SOLUBILITY IN VARIOUS MEDIA

Objective: This study seeks to explore the solubility of an antidiabetic drug with poor water solubility, Repaglinide (RPG), in various media, and develop and optimize RPG-loaded spanlastic formulations. Methods: A 72-hours solubility study was conducted for RPG in different media, followed by UV spectrum analysis. Spanlastics containing RPG were prepared through the thin-film hydration (TFH) method combined with ultrasonication, incorporating varying concentrations ofthe lipophilic, non-ionic surfactant agents, Span 60 and Tween 80. Characterization was performed To assess particle size (PS), polydispersity index (PDI), zeta potential (ZP), and entrapment efficiency (EE%). Optimization was achieved by analysing characterization results and using a mixture design of Design Expert software which predicted the optimized formula. Results: RPG exhibited the highest solubility in phosphate buffer saline (PBS, pH 6.8) incorporating 0.5% Tween 20 (168.595 µg/mL), followed by PBS with 0.1% Tween 80 (80.355 µg/mL), PBS alone (56.163 µg/mL), and PBS with 0.1% sodium lauryl sulphate (SLS) (53.706 µg/mL). UV scanning in methanol revealed three characteristic absorption bands for RPG, with peak selection optimized for molar absorptivity in each medium. The RPG-loaded spanlastic formulations demonstrated nano-sized vesicles with uniform size distribution, high stability, and efficient drug encapsulation. Optimized spanlastics predicted a particle size 126.162 nm, PDI 0.416, ZP-43.258 mV, and EE% 77.753%. Conclusions: This research emphasizes the potential of optimized RPG-loaded spanlastics, developed through the thin-film hydration method, as a promising approach for improving the solubility and stability of poorly water-soluble drugs. RPG showed the highest solubility in PBS (pH 6.8) containing 0.5% Tween 20, and the formulation achieved desirable nanosize, uniformity, and high encapsulation efficiency. Peer Review History: Received 12 July 2024; Reviewed 16 September 2024; Accepted 23 October; Available online 15 November 2024 Academic Editor: Dr. Asia Selman Abdullah, Pharmacy institute, University of Basrah, Iraq, asia_abdullah65@yahoo.com Average Peer review marks at initial stage: 6.0/10 Average Peer review marks at publication stage: 7.5/10 Reviewers: Antonio José de Jesus Evangelista, Federal University of Ceará, UFC, Brazil, tony_biomed@hotmail.com Asmaa Ahmed Mohamed Ahmed Khalifa, Pharos University, Alexandria, Egypt, asmaa.khalifa@pua.edu.eg

Plasma synthesis and characteristics of nanocomposite coatings based on PVA and chitosan with incorporated nanoparticles for the healing of skin wounds

This study investigated the impact of new composites based on PVA and chitosan on wound healing in mice. Metal or metal oxide nanoparticles were synthesized without reagents by creating an impulse discharge between metal electrodes in a polymer dispersion. Polymer composites and coatings were then prepared using these nanoparticles. The composites were analyzed using various techniques, including UV spectroscopy, X-ray diffraction, scanning and transmittance electron microscopy, and infrared spectroscopy. The maximum concentration of nanoparticles in the composite was 3.5%. The average diameter of the nanoparticles was approximately 50-60 nm. The inclusion of Ag and ZnO nanoparticles accelerated the healing process by approximately 25%.

One-step preparation of orange-red Emitting carbon dots for visual detection of copper ions in the water environment

In this study, p-aminophenol and o-phenylenediamine were used as materials in a straightforward one-step hot solvent approach for generating a nitrogen-doped carbon dot. Fourier Transform Infrared Spectroscopy (FT-IR), Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), X-ray Diffraction (XRD), and UV–Vis analysis were used to analyze the resultant CDs composites. Furthermore, fluorescence and absorption spectroscopy were used to assess the optical characteristics. Following the addition of Cu2+, CDs’ fluorescence was quenched statically, resulting in the formation of a non-fluorescent complex. Based on this, a highly selective fluorescent technique was developed with a detection limit of 2.71 μM and a relevant linear range of 10–100 μM for Cu2+. More convenient to carry and detect sodium alginate gel beads doped with carbon dots were prepared for visual detection of Cu2+. Using this method, Cu2+ ions from various environmental sources can be detected rapidly, cheaply, environmentally friendly, and selectively.

Characterization and Ambient Temperature Hydrogen Sulfide Sensing of Annealed NiO-MnO₂ Thin Films Prepared via Jet Nebulizer Spray Pyrolysis

A jet nebulizer spray pyrolysis technique to synthesize NiO-MnO2 thin films was demonstrated. These nanosheets were annealed at 350, 450 and 550 °C for 2 hours. The thin films surface morphology and structure were characterized using scanning electron microscopy, XRD, and UV spectroscopy. Subsequently, utilized as gas sensors for the detection of hydrogen sulphide gas. The introduction of NiO into MnO2 nanosheets significantly improved the gas-sensing performance. Notably, the gas-sensing response of the NiO-MnO2 nanosheet sensor surpassed that of both NiO and MnO2 alone, reaching a notable value of 165 when detecting 100 ppm hydrogen sulfide gas with the NiO-MnO2 thin film sensor. A remarkable feature of the NiO-MnO2 thin film sensor is its accelerated response time, registering at 10 seconds when exposed to 100 ppm of hydrogen sulfide gas. The mechanism underlying gas sensing and the factors contributing to the enhanced gas response of NiO-MnO2 thin film is thoroughly discussed. From a broader perspective, these developed sensors present a novel platform for the identification and monitoring of hydrogen sulfide gas, showcasing significant advancements in gas-sensing technology.

Integration and Characterization of Synthetic Biodegradable Polymer (PVA) with Graphite Oxide (GO) for Performance Assessment in Sustainable Electrochemical Devices

AbstractIn recent years, the demand for sustainable materials in electrochemical devices has driven the exploration of innovative composites. This study focuses on the integration and characterization of synthetic biodegradable polymer polyvinyl alcohol (PVA) with graphite oxide (GO) to evaluate their performance in sustainable electrochemical applications. PVA, known for its biodegradability and biocompatibility, was combined with GO to leverage its excellent electrical conductivity and large surface area. Microbial fuel cells (MFCs) represent a promising electrochemical biosynthesis technology that harnesses the enzymatic activities using microbes to produce energy from organic substrates. This renewable energy approach relies on the synergistic interaction between electrochemically active bacteria and electrode materials to facilitate electron transfer and power generation. Applications of MFCs range from wastewater treatment to sustainable power generation in remote or resource-limited settings. This study explores recent advances in MFC technology, challenges in scaling up for practical applications, and prospects for integrating MFCs into renewable energy strategies. The nano composite membrane was evaluated for structural, morphological, crystalline, and thermal properties by using Fourier Transform Infrared Spectroscopic (FTIR), Scanning Electron Microscopy (SEM), X-Ray Diffraction (XRD), and UV- visible spectroscopy. Additionally, the biodegradability of the composite was assessed, confirming that it maintains its environmental benefits while offering improved performance for potential applications in sustainable energy storage and conversion devices. This work provides a promising avenue for the development of eco-friendly electrochemical devices with optimized performance characteristics.

Preparation and characterization of a multifunctional film with excellent UV shielding, antibacterial and antioxidant capabilities containing cinnamaldehyde and titanium dioxide (TiO2)

AbstractSoy protein isolated (SPI) have a wide application in the food packaging, but their water resistance, mechanical properties and antibacterial properties are not ideal for directly use. In our study, we developed a series of SPI composite films loaded with cinnamaldehyde and titanium dioxide (TiO2). X‐ray diffraction (XRD), UV scanning spectra (UV) and spectro colorimeter were applied to indicate the structure of films. The incorporation of TiO2 (0.5%) caused a subsequent effect on the tensile property, UV blocking properties and water vapor properties (WVP). On the other hand, SPI film composed with a constant amount of TiO2 and different concentration of cinnamaldehyde showed an excellent synergistic effect to improve the tensile property, moisture content, UV blocking properties, water vapor properties and morphological characteristics. Furthermore, the composite films exhibited superior antibacterial activity when tested against both S. aureus and E. coli. The microstructure of the composite film become heterogeneous and rough after addition of TiO2 and cinnamaldehyde compare to control film. The findings of the present study may facilitate a re‐evaluation of the potential of using multiple compounds to prepare active edible films.

HPTLC sequentially coupled to UV and SERS: A cost-effective tool for confirmative identification and quantitation of sildenafil in falsified herbal products

The detection of falsified drugs usually requires multi-disciplinary analysis for confirmative identification. Among hyphenated techniques with high specificity detection, thin-layer chromatography coupled with surface-enhanced Raman spectroscopy (TLC-SERS) is an efficient choice, especially for herbal products with diversified matrix. In this study, HPTLC was coupled to two detection techniques: UV absorption and Raman scattering with silver colloid enhancement for the analysis of sildenafil adulterated in herbal products. With this approach, orthogonal UV and SERS spectral data was collected, so that confirmative results could be obtained within a single TLC analysis. How this approach helped to reduce chances of false positive or false negative results was also discussed. The HPTLC sequentially coupled to UV and SERS (HPTLC-UV-SERS) method was developed and validated parallelly on the UV and SERS signals. To improve the repeatability of the SERS signal, several analytical conditions were optimized, so that direct quantitation with TLC-SERS was feasible without chemometric data extrapolation. The determination was done with UV scanning at 304 nm for HPTLC and with SERS signal at 1580 cm−1 (excitation 633 nm). The TLC-SERS method had a detection limit of 1.65 ng/spot, 95 times lower than HPTLC method (157 ng/spot). The HPTLC-UV-SERS method was applied on 24 real herbal samples collected from the market, among which 3 real samples were positive to sildenafil, and quantitation results by UV and SERS were in consistency. Not only this method was proved feasible for practical applications, but the recommendations for TLC-SERS procedures could also be useful in TLC-SERS method development for other compounds.

Withdrawal: Yang, M.-N., Zhang, Y.-F., Zhi, G.-Y., Gu, X.-F., Han, L. and Zhang, D-H. (2020), Fabricating cholyglycine-glucose-6-phosphate dehydrogenase conjugates for cholyglycine detection. Biotechnology and Applied Biochemistry. (Accepted Article): https://doi.org/10.1002/bab.1983.

To establish cholyglycine (CG) detection via enzyme multiplied immunoassay technique (EMIT), glucose-6-phosphate dehydrogenase (G6PD) was used as a reporter enzyme to prepare hapten-enzyme conjugate. Gel electrophoresis and UV scanning demonstrated that G6PD was successfully labeled with cholyglycine and CG-G6PD conjugate was obtained. Furthermore, the effects of various parameters on the preparation of CG-G6PD conjugates were investigated. Consequently, CG amount, NADH, D-glucose-6-phosphate (G6P), phosphate buffer and the pH, and ionic strength of solution had important effects on the residual activity of CG-G6PD. Moreover, CG amount, the pH, and G6P played important roles in changing CG labeling location on G6PD. Using the CG-G6PD conjugate as test kit, the cholyglycine-EMIT calibration curve was established, which could be employed in clinical detection of cholyglycine. This study provides some valuable information for preparing hapten-G6PD conjugates. This article is protected by copyright. All rights reserved.

Synthesis, characterization and photocatalytic activity of alginate based hybrid material for efficient degradation of organic pollutants under UV light and sunlight irradiation

This study reports the development of alginate based hybrid material (Alg/CuO-gC₃N₄) with copper oxide (CuO) and graphitic carbon nitride (gC₃N₄). The Alg/CuO-gC₃N₄ hydrogel bead was prepared by two step process is as follows: formation of CuO-gC₃N₄ by co-precipitation method and incorporation of CuO-gC₃N₄ in the calcium alginate (Alg) by ionotropic gelation method. The structural and morphological features of the Alg/CuO-gC₃N₄ was characterized using different techniques namely Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Thermogravimetric analysis (TGA), UV Diffuse Reflectance spectroscopy (UV-DRS) and Scanning electron microscopy (SEM). The photocatalytic activity of the Alg/CuO-gC₃N₄ was extensively evaluated by degrading methylene blue (MB) under UV–vis and sunlight irradiation. The influence of various parameters like pollutant type, initial dye concentration, catalyst dosage, effect of pH, light intensity and reusability was investigated. The Alg/CuO-gC₃N₄ achieved a maximum degradation efficiency of 86.26 % and 85.15 % for MB at a dye concentration of 1 × 10−5 M under UV–vis and sunlight irradiation respectively, within 60 min using 0.3 wt% of catalyst dosage. The Alg/CuO-gC₃N₄ exhibited excellent stability and reusability over multiple cycles. Additionally, the incorporation of CuO-gC₃N₄ in alginate facilitates in reducing the bandgap (2.54 to 2.28 eV) for efficient charge separation, further enhancing the overall photocatalytic activity.

Boosting the visible-light-driven photocatalytic efficiency in porous Cu/TiO2 ceramic coatings

In this study, we examined how current density, sodium phosphate concentration, and the addition of copper sulfate to the supporting electrolyte affect PEO coatings on pure titanium. The chemical composition, morphology, and optical behavior of PEO coatings were extensively analyzed using scanning electron microscopy (SEM), X-ray diffraction (XRD), and UV–Vis diffuse reflectance spectroscopy (DRS). With an increase in current density from 6 to 18 A/dm2, the coating's pore size enlarges and the rutile phase content within the coatings increases. The coating created at 12 A/dm2 shows the highest photocatalytic activity and triggers a unique three-stage behavior in the voltage-time diagram. Adding 4 g/l of CuSO4 to the electrolyte resulted in the formation of an unwanted Cu-based precipitates, causing electrolyte instability. To stabilize it, potassium hydroxide was removed, and the base electrolyte concentration was adjusted. To address this issue, potassium hydroxide was eliminated from the supporting electrolyte. Subsequently, the concentration of the base electrolyte was optimized. Also, the synergistic influence of CuSO4 addition (4 g/l) to the supporting electrolyte at different concentrations of 5, 10, and, 15 g/l of trisodium phosphate on PA was investigated. The superior photocatalytic performance of the optimum coating created in the electrolyte containing 5 g/l trisodium phosphate and 4 g/l CuSO4 is attributed to its enhanced porous structure, along with the heterojunction between the anatase and rutile phases. These features significantly boost the available surface area and facilitate the efficient separation and transfer of photogenerated charge carriers. Ultimately, a mechanism for MB photodegradation was proposed based on the findings from Mott-Schottky analysis and DRS.

Optical properties and band-gap engineering of group (I) element-doped ZnO nanoparticles as P-type semiconductors

This study investigated the optical properties and band-gap engineering of zinc oxide (ZnO) nanoparticles doped with Group I elements, focusing on their potential as p-type semiconductors.Zn0.95M0.05O (M = Li, Na, and K) nanoparticles were synthesized by a gelatin-based sol-gel method.Through a combination of experimental characterization techniques including field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), and UV–vis spectroscopy, the crystal morphology, structure, and optical properties of the doped ZnO nanoparticles were analyzed. The Kramers-Kronig (K–K) method was employed to evaluate the behavior of the refractive index and optical dielectric function, which are complex quantities. The incorporation of Group I dopants into the ZnO lattice is explored for its impact on the band-gap structure and the realization of p-type conductivity.

Optical and photoluminescence properties of trivalent rare earth ions doped LiMgPO4

Trivalent rare-earth-activated luminescent materials have attracted a lot of attention due to their fascinating optical properties and broad range of applications. In this study, polycrystalline Sm and Eu-doped LiMgPO4 luminescent materials were synthesized using a solid-state reaction technique. The crystalline structures, morphological features, and optical characteristics of the synthesized materials were thoroughly investigated using x-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Field-Emission Scanning Electron Microscopy (FE-SEM) and UV–vis spectroscopy. The Rietveld refinement analysis confirmed the phase purity of synthesized phosphors. In addition, the strain induced owing to the incorporation of trivalent rare-earth ions and crystallite sizes of each synthesized material was estimated using the Williamsons-Hall (W-H) and modified Debye-Sherrer equations. The estimated results of the microstrain were reported to be 0.0010, 0.0023 and 0.0020. Subsequently, as a consequence of distinct peak profile options, the projected average crystallite size by both approaches was distinct. The UV–vis spectroscopy analysis reveals that Sm and Eu doped materials exhibit absorption bands around 402 nm and 396 nm and depicts the declining band gap values. The Photoluminescence (PL) spectra at 402 nm and 395 nm excitation wavelengths exhibited the distinctive emissions of both Sm3+ (4G5/2 → 6H7/2) and Eu3+ (5D0 → 7F2) ions emanating due to the intra-configurational f - f and 4 f − 4 f transitions. Finally, the CIE diagram explicitly demonstrates that the Sm and Eu-modified materials reveal their exceptional color purity. McCamy’s approach was used to determine various parameters of trivalent rare-earth doped materials. The correlated color temperature ( TCCT ) and their coordinates TCCT (ζ, β) was found to be 2287 K, 1900 K, 0.281, 0.632, and 0.302, 0.681 respectively. Temperature-dependent emission spectra exhibit excellent color and thermal stability at high temperatures. The obtained results in this study indicate that the synthesized luminescent materials can potentially be used in white light-emitting diode applications.

Synthesis of Au‐decorated WO3 nanoplates for simultaneous oxidation of RhB and reduction of Cr(VI) under visible light irradiation

AbstractTungsten oxide (WO3) nanoplates were synthesized by a simple chemical precipitation technique. These nanoplates were decorated by Au nanoparticles via photoreduction route. The obtained samples were characterized by field emission scanning electron microscopy, X‐ray diffractometry and UV–vis diffuse reflectance spectroscopy to investigate their morphological, structural and optical properties. The SEM images show the plate morphology with the size in the range of 50–150 nm, their thickness ≈10 nm; spherical‐like Au nanoparticles ≈20 nm were attached to the surface of the WO3 plates. These materials were applied for the simultaneous photocatalytic degradation of Rhodamine B (RhB) and Cr(VI) under visible light. Due to the efficient separation of photoinduced charge carriers and the enhanced visible light absorption ability, the photocatalytic performance of Au‐decorated WO3 nanoplates was higher. The main active species responsible for the photodegradation reactions were identified by different scavengers and a photocatalytic mechanism was proposed. This work represents that WO3 nanoplates are a promising candidate for the degradation of organic and inorganic pollutants from the industrial wastewater under sun light exposure.

Synthesis of Zinc Oxide Nanoflakes by Non-Thermal Plasma Technique and Evaluation of Anticancer Activity in Vitro

This paper describes the synthesis of ZnO nanoparticles using air microplasma jets as the main reaction medium. Various techniques, including X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM) and UV—Vis spectroscopy, were employed to confirm the characterization of the artificially synthesized ZnO nanoparticles. The absence of additional peaks associated with secondary phases of ZnO, as revealed by the XRD analysis, indicates the exceptional purity of these nanoparticles. Through the use of SEM, we examined the surface morphology and observed a substantial rate of agglomeration. The energy band gaps of ZnO nanoparticles were determined to be 3.3 electron volts (eV). The cytotoxic effects of zinc oxide nanoparticles were assessed using the methyl thiazolyl tetrazolium (MTT) assay on breast cancer cells exposed to various doses of the nanoparticles. The Acridine-Orange/Ethidium-bromide (AO/EB) dual stain was employed to assess apoptotic cells. The data indicate that ZnO NPs can induce apoptosis, suggesting their potential as an anticancer treatment in breast cancer cell lines. The presented findings indicate that nanoscale zinc oxide particles (ZnO NPs) possess significant potential for future utilization in many biological applications. An example of such an application could be the prospective substitution for chemotherapy in the management of various forms of cancer diseases.

Molecularly imprinted orthophenylenediamine with Ti3C2TxMXene‐based biosensor for sensitive detection of bilirubin

AbstractIn this study, bilirubin (BR) is detected by using a novel molecularly imprinted electrochemical sensor based on a Ti3C2TxMXene‐modified ITO electrode. First, Ti3C2TxMXene is synthesized by chemical etching and deposited at the ITO electrode surface by drop casting. After that, ortho‐phenylenediamine (o‐PD) as monomer is electropolymerized in the presence of a sodium acetate buffer solution containing the BR template to prepare BR‐imprinted electrode. Field emission scanning electron microscopy (FESEM), x‐ray diffraction and UV–visible absorption spectroscopy confirms the MXene synthesis. The molecular imprinted polymer (MIP) formation at the electrode is confirmed by electrochemical methods such as differential pulse voltammetry, electrochemical impedance spectroscopy, and cyclic voltammetry, and FESEM. The linearity range, limit of detection and the limit of quantification are calculated as 0.1 mg/dL to 20 mg/dL, 0.002 mg/dL, and 6.6 mg/dL respectively. Stability and reproducibility are also reported for the prepared MIP sensor.

Exploring the photocatalytic degradation of organic textile dye using hydrothermally synthesized Ce-doped metal chalcogenide nanomaterial

The advancement of economical, facile and non-toxic approaches towards waste water treatment is a promising research area currently among scientific community. In this regard photocatalysis has specifically received significant attention due to its application in the eradication of toxic elements from wastewater. Hence, in the present study Ce-doped FeSe2 photocatalysts were prepared employing the simple, inexpensive and ecofriendly hydrothermal method. The prepared Ce-FeSe2 nanomaterial was subjected to different characterization techniques like x-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), energy-dispersive spectroscopy (EDS), Cyclic Voltammetry (CV) and UV–visible spectroscopy to explore the characteristic properties of the prepared nanomaterials. XRD results revealed good crystallinity and purity of all synthesized Ce-FeSe2 nanomaterials. Surface morphology and elemental composition of the prepared nanomaterials was determined by FE-SEM and EDS spectroscopy. UV-Visible spectroscopy was used to explore the optical characteristic of the prepared photocatalysts. The results of optical absorption spectrum confirmed that increasing dopant percentage shows an improvement towards optical properties. The photocatalytic ability of the prepared Ce-FeSe2 nanomaterials was examined against the potentially toxic industrial dyes like Rhoadmine B (RB). The photocatalytic results show that 95.21% of dye degradation was exhibited by 7% Ce- doped FeSe2 nanomaterial under UV-visible light illumination duration of 120 min. Findings of the photocatalytic degradation process fitted well with pseudo first order reaction kinetics for RB dye.

Encapsulation and Characterization of Proanthocyanidin Microcapsules by Sodium Alginate and Carboxymethyl Cellulose.

Proanthocyanidins are important compounds known for their antioxidant and radical scavenging properties, but they are highly sensitive to light, heat, oxygen, and pH. In our study, proanthocyanidin was encapsulated using sodium alginate and carboxymethyl cellulose to enhance controlled release, pH stability, metal ion tolerance, temperature resistance, time release, the microencapsulation of food additives stability, antioxidant capacity analysis, and the storage period tolerance of proanthocyanidin. Fourier transforms infrared (FTIR) analysis and full-wavelength UV scanning indicated the successful immobilization of proanthocyanidins into the polymeric microcapsules. The flowability and mechanical properties of the microcapsules were enhanced. Moreover, proanthocyanidin microcapsules exhibited higher thermal, pH, metal ion, time, and microencapsulation food additive stability. In addition, due to their high antioxidant properties, the proanthocyanidin microcapsules retained a greater amount of proanthocyanidin content during the gastric phase, and the proanthocyanidin was subsequently released in the intestinal phase for absorption. Thus, the study provided a systematic understanding of the antioxidant capabilities and stability of proanthocyanidin microcapsules, which is beneficial for developing preservation methods for food additives.