Sauropus Androgynus Research Articles

Effect of Katuk Leaves Ethanol Extract Gel as an Antibacterial and Antifungal in Orthodontic Treatment

Fixed orthodontic treatment is one option for treating malocclusion. The intricate design makes it difficult for patients to clean their teeth and can cause the emergence of microorganisms in the oral cavity. Katuk plant (Sauropus androgynus (L.) Merr.) is commonly used as herbal medicine. Flavonoids in katuk leaves have antimicrobial properties. The purpose of this study was to determine the effect of katuk leaf ethanol extract gel as an antimicrobial by inhibiting the growth of bacteria and fungi involved in orthodontic treatment. The study was an experimental study with a post-test-only control group using katuk leaves ethanol extract gel formulation as treatment, aquadest gel as a negative control, ketoconazole as a positive control against Candida albicans, and amoxicillin against Streptococcus mutans. Data were analyzed using the Kruskall– Wallis and Mann–Whitney tests (P < 0.05). The results show that katuk leaves have inhibition on Streptococcus mutans (P = 0.000) growth but have no inhibition on Candida albicans (P = 1.00) growth. The conclusion of this study is that the gel of katuk leaves ethanol extract (S. androgynus (L.) merr.) has antibacterial activity against S. mutans in the oral cavity but no antifungal effect on C. albicans. Keywords: Candida albicans, fixed orthodontics, katuk leaves, Streptococcus mutans

Katuk Leaves (Sauropus androgynus (L.) Merr) on Hemoglobin Level among Pregnant Women in Third Trimester

Background: In 2018, 41,8% of amternal mortality in developing countries was caused by anemia in pregnancy, the prevalence of anemia in Indonesia is still very high at 44,2%. One of the causes of anemia is a lack of iron in the body. Anemia can be prevented by consuming foods that contain iron and vitamin C to help the absorbtion process, by consuming katuk leaves while continuing to consume Fe Tablets according to government recommendations.Purpose: This study aims to determine the effect of Katuk Leaves (Sauropus androgynus) and Fe Tablets on hemoglobin levels among pregnant women in third trimester.Methods: This research is aquassy experiment pre and post test with control group design. The population in this study was 86 third trimester pregnant women which 45 had anemia in last 6 month at Private Midwifery Practice N. The sample were divided by intervention group and cotrol group, each group was 15 pregnant women, according to sample theory of experimental research. The sampling technique used purposive sampling. The research instrument used observation sheets and Digital Hemoglobinometer. The data analyzed with parametric test, the t-test.Results: The result of statistic tests showed that there was an effect of katuk leaves and Fe tablets on hemoglobin level with p value of 0,00 (0,005).Conclusion: Consumption katuk leaves and Fe tablet is more effective in changing hemoglobin levels compared to consuming Fe tablets only.

Physicochemical characterization, antioxidant and antimicrobial activities of chitosan film containing Sauropus androgynus extract prepared by solution casting and supercritical impregnation

Sauropus androgynus extract (SAE) was incorporated with chitosan to produce solution casting film (SAE-SCF) and supercritical impregnation film (SAE-SIF). The physicochemical characterization and biological activities of SAE-SCF and SAE-SIF films were evaluated and compared. In terms of total phenolic content (TPC) and total flavonoids content (TFC), SAE-SCF (TPC: 49.14–71.88 mg GAE/g; TFC: 32.73–57.05 mg QE/g) obtained higher results than SAE-SIF (TPC: 8.40–18.90 mg GAE/g; TFC: 1.97–12.34 mg QE/g). FTIR results confirmed the interaction between SAE-active compounds and chitosan via hydrogen bonding and electrostatic interaction. Due to the formation of hydrogen bonding or cross-linking effects in SAE-chitosan film, the light opacity (1.748–3.375 AU), water vapor permeability (2.87–3.92 × 10−11 g m−1.s−1.Pa−1), and mechanical properties (tensile strength; 8.65-15.72 MPa; elongation at break: 7.95–13.25%) were enhanced compared to neat chitosan film, with SAE-SCF showing higher performances than SAE-SIF. The antioxidant activities of both films were evaluated using ABTS (IC50: 55.98–97.85 μg/mL) and DPPH assays (IC50: 53.68–143.04 μg/mL), with SAE-SCF showing higher inhibition of free radicals than SAE-SIF. The ferric-reduction power was recorded (28.17–94.95 Trolox μmol/mL), and SAE-SCF achieved a higher reduction power than SAE-SIF. In antimicrobial analysis, SAE-SCF demonstrated the best reduction of viable growth of all tested foodborne microorganisms. Overall, the functional performance of SAE-SCF is better than SAE-SIF in all tested aspects. The results revealed the potential of SAE-SCF to be utilized as a food packaging material.

Leaf growth pattern and morphology of Sauropus androgynus (L.) Merr. in tropical lowland

Chikurmanis or katuk (Sauropus androgynus (L.) Merr.) is a traditional leafy vegetable that is consumed widely in Indonesia. The leaf is rich in vitamins, minerals, nutrients, and dietary fiber. The study aimed to investigate the leaf growth pattern and morphological characteristics of the chikurmanis cultivated in the tropical lowland ecosystem. The research was conducted on local farmland in Marga Sakti Sebelat, Bengkulu, Indonesia on December 2023 by survey method. The results showed that the leaf of chikurmanis grew in the daytime and continued to grow at nighttime as indicated in midrib length, leaf width, and foliole number. It is likely that nighttime growth become predominant in chikurmanis. The plant was a type of shrub with complete organs of stem, branch, root, flower, and leaf. The leaf was arranged as a compound leaf with many folioles in opposite positions. Midrib length (M) x leaf width (W) with a zero intercept of linear regression was the most reliable predictor of leaf area with the formula Y = 0.4964MxW (R2 = 0.9677). Keywords: chikurmanis plant; katuk; leaf area estimation; morphological trait; traditional leafy vegetable

Formulation and Evaluation of Face Moisturizing Cream from Katuk Leaf Extract (Sauropus Androgynus Merr)

The Katuk plant (Sauropus androgynus L. Merr) is a natural substance with proven efficacy in treating diabetes, obesity, and inflammation. It also possesses antioxidant, lactation-inducing, and antibacterial properties. This study aimed to investigate the impact of varying concentrations of katuk leaf extract on characteristics of the moisturizer cream produced. The study involved extracting katuk leaves using the maceration method for 24 hours, utilizing ethanol as the solvent. The extraction process resulted in a yield of 1.86%. The final yield contains alkaloid chemicals, flavonoids, terpenoids, saponins, and tannins. The formulations employed in this investigation utilized the codes F0, F1, F2, and F3, representing the content of katuk leaf extract at 0%, 2%, 4%, and 6%, respectively. The cream generated exhibited an identifiable aroma of katuk and had a hue ranging from light green to blackish brown, as determined by organoleptic testing. The pH values of specimens F0, F1, F2, and F3 were 7, 7, 7, and 8, respectively. The spread ability measurements for specimens F0, F1, F2, and F3 were 5.3 cm, 5.5 cm, 6 cm, and 6.1 cm, respectively. According to the findings of this investigation, specimens F0, F1, F2, and F3 adhered for 5 seconds, 4 seconds, 4 seconds, and 5 seconds, respectively. The formulation F2 is the most superior product generated in this investigation. This is because this specimen exhibits the highest level of respondent satisfaction compared to the other specimens. This formulation exhibits antioxidant activity with a per cent inhibition of 30.51% and an IC50 value of 84.63 ppm. The face moisturizing cream derived from katuk leaf extract possesses a high antioxidant potency, placing it in the active/strong category.

The Effect of Using Katuk Leaves (Sauropus Androgynus (L) Merr) As A Substitute for ZA (Ammonium Sulfate) on the Yield, Thickness, Color, Texture, and Organoleptic of Nata De Whey

Utilization of cheese waste, namely whey, is one alternative to increase the added value of whey by processing it into nata de whey. This study aims to determine the effect of using Katuk leaves (Sauropus androgynus (L) Merr) as a substitute for ZA (Ammonium Sulfate) on the yield, thickness, color, texture, and organoleptic of Nata de whey. This study used 8 liters of whey and Katuk leaves (Sauropus androgynus (L) Merr) as much as 600 g. The experimental design used in this study, namely the Randomized Block Design (RBD) with four treatments and five repetitions. The treatment in this study was A = 0.5% ZA, Katuk leaves (B = 5%, C = 10%, and D = 15%). The observed variables were yield, thickness, color, texture, and organoleptic. The results showed that the treatment differed significantly (P<0.05) in yield, thickness, color, texture, and organoleptic. The best treatment result is treatment B with a yield value of 91.94%, thickness of 7.77 mm, lightness (L*) 59.17, greenness (a*) -4.16, yellowness (b*) 7.52, texture 5.25 N/cm2, organoleptic color 3.90, scent 3.58, taste 3.82, texture 3.78.

Haplotype-resolved genomes of octoploid species in Phyllanthaceae family reveal a critical role for polyploidization and hybridization in speciation.

The Phyllanthaceae family comprises a diverse range of plants with medicinal, edible, and ornamental value, extensively cultivated worldwide. Polyploid species commonly occur in Phyllanthaceae. Due to the rather complex genomes and evolutionary histories, their speciation process has been still lacking in research. In this study, we generated chromosome-scale haplotype-resolved genomes of two octoploid species (Phyllanthus emblica and Sauropus spatulifolius) in Phyllanthaceae family. Combined with our previously reported one tetraploid (Sauropus androgynus) and one diploid species (Phyllanthus cochinchinensis) from the same family, we explored their speciation history. The three polyploid species were all identified as allopolyploids with subgenome A/B. Each of their two distinct subgenome groups from various species was uncovered to independently share a common diploid ancestor (Ancestor-AA and Ancestor-BB). Via different evolutionary routes, comprising various scenarios of bifurcating divergence, allopolyploidization (hybrid polyploidization), and autopolyploidization, they finally evolved to the current tetraploid S. androgynus, and octoploid S. spatulifolius and P. emblica, respectively. We further discuss the variations in copy number of alleles and the potential impacts within the two octoploids. In addition, we also investigated the fluctuation of metabolites with medical values and identified the key factor in its biosynthesis process in octoploids species. Our study reconstructed the evolutionary history of these Phyllanthaceae species, highlighting the critical roles of polyploidization and hybridization in their speciation processes. The high-quality genomes of the two octoploid species provide valuable genomic resources for further research of evolution and functional genomics.

Volatile Compounds Content and Sensory Profile of Katuk (Sauropus androgynus) Leaves After Household Scale Heating

Katuk (Sauropus androgynus) is an indigenous Indonesian plant, and the leaves are often consumed as fresh or processed vegetables. Despite its potential, there are no reports on the volatile compounds content and sensory profile of Katuk leaves after being processed using household scale heating. Therefore, this study aimed to determine the volatile compounds content and sensory profiles of Katuk leaves after household scale heating (steaming and boiling). The extraction of constituents was carried out using the headspace-solid phase microextraction method, followed by identification with gas chromatography-mass spectroscopy. The sensory profile analysis was performed using the free choice profiling method with untrained panelists. The analysis results showed the presence of 16 volatile compounds derived from 7 groups, including aldehydes (5 compounds), alcohol (3 compounds), other components (3 compounds), ketones (2 compounds), as well as benzene, terpenoids, and esters (1 compound). The dominant compounds found in fresh Katuk leaves were alcohol [(Z)-3-hexene-1-ol]. Meanwhile, aldehydes (benzaldehyde, nonanal, benzeneacetaldehyde, and (E)-2-pentenal) and alcohol (1-Heptanol and Nerolidol) were dominant in the steamed samples, with benzene (naphthalene) being predominantly found in the boiled samples. The sensory profile analysis results showed that fresh Katuk leaves had a grassy and earthy aroma with a grassy flavor, while the boiled samples had a smooth and juicy texture. The dominant attributes found in steamed Katuk leaves included moist, tender, and tasteless. Based on these findings, household scale heating could modify the composition of volatile compounds, thereby affecting the sensory profile. The results obtained were expected to serve as a foundation for the processing of Katuk leaves at both industry and household levels.

Synergistic modulation of proinflammatory mediators and cytokines in lipopolysaccharide-activated RAW 264.7 macrophages: The therapeutic potential of Elephantopus scaber and Sauropus androgynus ethanol extract

Background and Aim: Elephantopus scaber (ES) and Sauropus androgynus (SA) have broad biological effects and have long been used in traditional medicine. However, the anti-inflammatory properties of the combination of ES and SA have not yet been fully explored. This study aimed to investigate the anti-inflammatory activities of the combination of ES and SA ethanol extract on lipopolysaccharide (LPS)-activated RAW 264.7 macrophage cell lines by inhibiting proinflammatory mediators and cytokines. Materials and Methods: Nitric oxide (NO) production in RAW 264.7 cells was assessed using the Griess protocol. The effects of the combination of ES and SA ethanol extract on RAW 264.7 cell viability were determined using WST-1 (4-[3-(4-Iodophenyl)-2-(4-nitro-phenyl)-2H-5-tetrazolio]-1,3-benzene sulfonate) assay. The levels of proinflammatory cytokines, including interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α), and interleukin-1 beta (IL-β), as well as the production of inducible nitric oxide synthase (iNOS), were assessed using flow cytometry. Results: This study demonstrated that ES and SA have excellent NO, iNOS, and proinflammatory inhibitory activities on LPS-induced RAW 264.7 macrophages. The formula ratio of 2ES:1SA showed the best NO inhibitory activity without any cytotoxicity, whereas the higher dose of SA (1ES:2SA) showed the best suppression of iNOS and proinflammatory cytokines IL-1β, IFN-γ, and TNF-α. Conclusion: The combination of ES and SA ethanol extract could be an alternative agent for reducing excessive inflammation in inflammatory diseases. Keywords: Elephantopus scaber, inflammation, macrophage, nitric oxide, proinflammatory cytokines, Sauropus androgynous.

The Effect of Katuk Leaf Soup (Sauropus Androgynus) on the Amount of Breast Milk of Postpartum Mothers at PMB Ade Mila Marliana, Babakan Jawa, Majalengka in 2023

Breast milk is the best liquid for newborns until the age of 6 months, because the components of breast milk are easily digested and absorbed by the baby's body. There are many ways to increase breast milk, one of them is by consuming traditional medicinal plants, namely katuk leaf. The purpose of this research is to determine the effect of katuk leaf soup on the amount of breast milk for postpartum mothers at PMB Ade Mila Marliana, SST. This research used pre-experimental design with one-group pre-post test design. The population in this research was all postpartum mothers who gave birth at PMB Ade Mila Marliana, S.ST for the period April-May 2023 as many as 20 people. Sampling in this research using total sampling technique. Data analysis includes univariate analysis using frequency distribution and bivariate analysis using the Wilcoxon test. The results of the analysis using Wilcoxon obtained a p value of 0.000 (<0.05). The conclusion of this research is that there is an effect of katuk leaf (Sauropus Androgynus) on the amount of breast milk for postpartum mothers at PMB Ade Mila Marliana, SST Kelurahan Babakan Jawa, Kabupaten Majalengka. This research is expected to be an input for further researchers to develop research related to the effect of katuk leaf on the amount of breast milk for postpartum mothers.

Eksplorasi Pengetahuan lokal Etnomedisin Daun katuk (Sauropus Andíogynus (L.)Meeí) Booster ASI pada Ibu Menyusui di Kelurahan Sampara Kabupaten Konawe

Providing breast milk (ASI) is very important for optimal growth and development of babies. One of the efforts that can be made to increase breast milk production is with herbs such as katuk extract (Sauropus androgynus (L.) Merr.) Knowledge about the use of plants as medicinal ingredients used in various ethnic groups in Indonesia has not been utilized properly. Ethnomedicine studies are a scientific way to document the use of plants as medicinal ingredients in various tribes. The aim of this research is to find out the form of the herb, how to process it, how to use it and the factors behind people using katuk leaves (Sauropus androgynus (L.) Merr) as a breast milk booster in the Sampara sub-district. The research method used was qualitative descriptive observational. The sampling technique is purposive sampling through observation and in-depth interviews with informants. The results of the research showed that participants said that the shape of the katuk leaf concoction was made like a clear vegetable with leaves shaped like Moringa, they did not know the taste of katuk leaves, received support and the cooking method used by the participants stated that before boiling, boil water, wash katuk leaves, add flavoring, then cooked for 3-5 minutes and then consumed during breastfeeding but the problem is that katuk leaves are difficult to buy.

Standardized mixture of Pluchea indica and Sauropus androgynus extract stimulates the gene expression associated with lactogenesis in rats

Pluchea indica (P. indica) and Sauropus androgynus (S. androgynus) are medicinal plants empirically used as an herbal galactogogue, but their mechanism of action has not been elucidated at the molecular level in the form of a mixed extract. This research aimed to standardize and investigate the combined effects of P. indica leaf extract (EPI) and S. androgynus leaf extract (ESA) on the expressions of prolactin (PRL), PRL receptor (PRLR), oxytocin (OXT), and OXT receptor (OXTR) genes in pituitary and mammary glands of lactating rats. The extracts were prepared by maceration in 70% ethanol. Standardization was done by examining non-specific parameters and determining total flavonoids using spectrophotometric analysis. Twenty-four lactating rats were divided into six groups. Group I: CMC 0.5% as control, II: EPI 500 mg/kgBW, III: ESA 125 mg/kgBW, and IV–VI were given combination formula EPI-ESA 1: 125+31.25, EPI-ESA 2: 250+62.5, and EPI-ESA 3: 500+125 mg/kgBW (orally, once a day) from the 2nd–15th day after parturition. On the 16th day, experimental animals were euthanized for tissue sampling, and gene expression was measured using real-time reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The study of the non-specific parameters revealed water content, total ash, and acid-insoluble ash met criteria based on the Indonesian Herbal Pharmacopoeia. Evaluation of total flavonoids yielded 7.33% ± 0.00% and 3.84% ± 0.01% w/w for EPI and ESA, respectively. Test results showed balanced induction of all target genes was achieved when subjects received EPI-ESA 2. We concluded that EPI-ESA can potentially support lactogenesis by stimulating the expressions of PRL, PRLR, OXT, and OXTR genes.

UJI AKTIVITAS ANTIDIARE TABLET EKSTRAK ETANOL DAUN KATUK (Sauropus androgynus L.) Merr. PADA MENCIT PUTIH (Mus musculus)

Diarrhea is a condition in which a person experiences excretion of feces more than 3 times a day accompanied by an increase in volume and dilu­­tion which results in dehydration and even causes death. The aim this study was to determine the antidiarrheal activity of ethanol extract tablets of katuk leaves (Sauropus androgynus L.) Merr. which contains tannins, flavonoids and alkaloids as anti-diarrheal compounds because they can inhibit intestinal motility. This test uses the intestinal transit method which measures the length of the intestine through which the marker passes. The number of mice used was 25 which were divided into 5 groups. The positive control group 1 used loperamide HCl at a dose of 4 mg/kgBW. Group 2 was negative control with tablets without extract, Group 3 with tablet extract 80 mg/kgBW, group 4 tablets with extract dose 160 mg/kgBW and group 4 with tablet extract 320 mg/kgBW. The results of this test show that in formulation 1 it has an activity value of 40.32%, in formulation 2 it is 43.21% and in formulation 3 it is 45.2%. Tablet preparations with the best results were in formulation 3 with an extract dose of 320 mg/kgBW which showed the best results

Klasifikasi Daun Herbal Menggunakan K-Nearest Neighbor dan Convolutional Neural Network dengan Ekstraksi Fourier Descriptor

The number of herbal plants in Indonesia is 30,000, but only about 1,200 plants are used in medicine. The large number of herbal plants makes it difficult for people to distinguish one type of herbal plant from another. From these conditions, this research has conducted tests to compare the performance of the K-Nearest Neighbor (KNN) and Convolutional Neural Network (CNN) methods using Fourier Descriptor (FD) feature extraction on herbal plants, namely moringa (moringa oleifera) and katuk (sauropus androgynus). The amount of data used is 480 data using image conditions, namely dark and light images which are then divided into 20% testing data and 80% training data. Classification is done using the KNN method using 5 distance calculations (Euclidean, Chebyshev, Manhattan, Minkowski, and Hamming) and CNN with FD feature extraction. From the tests that have been carried out, it is found that the use of FD feature extraction for the KNN method produces the best performance on both light and dark image data. While the use of the CNN method, for dark image data, the best accuracy results are obtained with FD feature extraction and CNN. Meanwhile, for bright image data, the best performance accuracy results are obtained in the CNN method without going through feature extraction. Of these three methods, using FD and KNN feature extraction is more recommended because it produces 100% accuracy in moringa and katuk images with light and dark intensity.

Polyploid Genome Assembly Provides Insights into Morphological Development and Ascorbic Acid Accumulation of Sauropus androgynus.

Sauropus androgynus (S. androgynus) (2n = 4x = 52) is one of the most popular functional leafy vegetables in South and Southeast Asia. With its rich nutritional and pharmaceutical values, it has traditionally had widespread use for dietary and herbal purposes. Here, the genome of S. androgynus was sequenced and assembled, revealing a genome size of 1.55 Gb with 26 pseudo-chromosomes. Phylogenetic analysis traced back the divergence of Sauropus from Phyllanthus to approximately 29.67 million years ago (Mya). Genome analysis revealed that S. androgynus polyploidized around 20.51 Mya and shared a γ event about 132.95 Mya. Gene function analysis suggested that the expansion of pathways related to phloem development, lignin biosynthesis, and photosynthesis tended to result in the morphological differences among species within the Phyllanthaceae family, characterized by varying ploidy levels. The high accumulation of ascorbic acid in S. androgynus was attributed to the high expression of genes associated with the L-galactose pathway and recycling pathway. Moreover, the expanded gene families of S. androgynus exhibited multiple biochemical pathways associated with its comprehensive pharmacological activity, geographic adaptation and distinctive pleasurable flavor. Altogether, our findings represent a crucial genomic asset for S. androgynus, casting light on the intricate ploidy within the Phyllanthaceae family.

In silico study of the flavonoid compound of Sauropus androgynus leaves ON RNA-Dependent RNA polymerase (RdRp) SARS-CoV-2

ObjectiveRNA-dependent RNA polymerase (RdRp) is a protein that is essential in the replication and transcription processes of SARS-CoV-2. RdRp inhibitors must be sought, particularly in the identification of active substances in herbal or human dietary sources. The purpose of this study was to investigate the molecular docking of phytochemistry from the leaves of Sauropus androgynus against the RdRp protein. MethodsThis in silico study was performed using AutoDock Tools 1.5.7, AutoDock Vina v1.2.3 software, and BIOVIA Discovery Studio Visualizer 4.1. ResultsAfzelin, kaempferol, and trifolin were found as phytochemistry in Sauropus androgynus leaves. Among the three flavonoid molecules, afzelin has the lowest negative binding affinity (−7.677 kcal/mol), followed by trifolin (−6.906 kcal/mol) and kaempferol (−6.65 kcal/mol). All three flavonoid compounds have a binding affinity that is more negative than the three conventional drugs (favipiravir, remdesivir, ribavirin). ConclusionsFlavonoid from the leaves of Sauropus androgynus leaves can be utilized as candidate for herbal or complementary medicine as an inhbitor of RdRp for COVID-19 treatment.

Alkaline humic acid fertilizer alters the distribution, availability, and translocation of cadmium and zinc in the acidic soil–Sauropus androgynus system

Humic acids (HA) are a popular soil additive to reduce metal availability, but they have the drawbacks of reduced effectiveness over time and a significant reduction in soil pH. An alkaline humic acid fertilizer (AHAF) combining alkaline additives with HA was developed to overcome such drawbacks. A field experiment was conducted to investigate the effects of different AHAF application rates on the physicochemical properties, bioavailability, accumulation, and translocation of Cd and Zn heavy metals in Sauropus androgynus grown in acidic soil. Based on our results, the 100AF (100% AHAF) treatment significantly increased soil pH, cation exchange capacity (CEC), and organic matter content (OM) after one year of application. Compared with the control treatment (CK), the application of different rates of AHAF resulted in a 37.1–40.3% decrease in soil exchangeable Cd fractions (Exc-Cd) and an increase in the humic acid-bound Cd fractions (HA-Cd) Fe- and Mn-oxide-bound Cd fractions (OX-Cd), and organic matter-bound Cd fractions (OM-Cd) by 9.5–64.6%, 24.8–45.1%, and 158.8–191.2%, respectively (P < 0.05). The different AHAF treatments decreased the Res-Zn, Exc-Zn, and OM-Zn fractions by 69.6–73.0%, 7.4–23.9%, and 18.1–23.2%, respectively (P < 0.05), and increased the HA-Zn fraction by 8.4–28.1%. In the control treatment, the bioconcentration factors (BCFs) for Cd and Zn in different S. androgynus plant organs were in the following order: (Cd) Leaves > Stems > Branches > Roots > Edible branches; (Zn) Roots > Stems > Leaves > Branches > Edible branches. The transfer factors (TFs) of Cd and Zn in S. androgynus were classified as follows: TF2 > TF1 > TF3 > TF4. Thus, S. androgynus stems, and roots had a strong ability to transport Cd and Zn to the leaves. Compared with CK, the 100AF treatment significantly increased the BCFs for Zn in all plant parts (except BCFedible branches). In contrast, it significantly decreased all BCFs and TFs for Cd and the TF4 for Zn, effectively reducing Cd and Zn accumulation in the edible branches of S. androgynus. Soil pH, CEC, OM, and HA-M fraction were highly and significantly negatively correlated with Cd and Zn content in edible branches (P < 0.001). Stepwise multiple linear regression analysis revealed that the soil HA-M fraction was the key contributing factor for Zn accumulation and translocation in S. androgynus. Moreover, based on our findings, the absorption, uptake, and translocation of Cd and Zn were mainly determined by metal speciation and the pH in the soil. Moreover, the competitive antagonistic mechanisms between Zn and Cd absorption also affected their accumulation in S. androgynus. Thus, AHAF can be used as a soil amendment to sustainably improve acidic soils and effectively reduce Cd and Zn accumulation in edible branches of S. androgynus.

Pengaruh Perbedaan Konsentrasi Propilenglikol terhadap Karakteristik Fisik Hair Tonic Ekstrak Daun Katuk (Sauropus androgynus (L). Merr)

Katuk leaf extract contains flavonoids which can stimulate hair growth by relaxing the muscles in the blood vessels around the hair follicles. One dosage form that is suitable for hair growth application is hair tonic preparations. An important component in the formulation of hair tonic is a thickener which can maintain the stability of the preparation. Propylene glycol is a thickening agent that can determine the physical characteristics of hair tonic preparations which are related to the stability of the resulting preparations. The purpose of this study was to determine the effect of different concentrations of propylene glycol on the physical characteristics of hair tonic katuk leaf extract (Sauropus androgynus (L.) Merr). This type of experimental research uses a completely randomized design (CRD) one factorial. The independent variables in this study were the concentration of propylene glycol 5%, 15% and 25%. Testing the physical characteristics of hair tonic preparations included organoleptic, clarity, pH, viscosity, and specific gravity. Data analysis used the ANOVA test with a 95% level of confidence then continued with the Post hoc test. The results of the physical characteristic test showed that the difference in the concentration of propylene glycol in hair tonic katuk leaf extract had an effect on the physical characteristics of viscosity (p&lt;0.05).

Effect of blanching, freezing and microwave-assisted pretreatment methods on chlorophyll extraction from Sauropus androgynus leaves

Sauropus androgynus is grown commonly in Vietnam, which has a high content of bioactive substances including chlorophyll, polyphenols, and antioxidant activity. Sauropus androgynus is used as food or a colorant for traditional Vietnamese cakes. Green pigments are employed in various industrial sectors, such as food, drinks, soap, and drug. Chlorophyll can be replaced synthetic green pigments that might be unhealthy. In this study, the effect of chlorophyll extraction efficiency and perceptional green color by extraction condition combinined with physical pretreatment methods was investigated using Sauropus androgynus leave as a model. Fresh raw materials were pre-treated by blanching, freezing and microwave treatment at 55w/g for 3 min and then extracted with 40% (v/v) ethanol solvent. The best chlorophyll recovery efficiency (CRE) from Sauropus androgynus by heat pretreatment methods with and without microwave assistance was 32.035% and 25.784% of chlorophyll content in material; or 1.081 mg/g d. w and 0.862 mg/g d. w of fresh leaves, respectively. The chlorophyll extract is then spray-dried to form a water-dispersible powder. Chlorophyll extracted with microwave-assisted pretreatment having high CRE that has potential applications in food production, but green color is not much more favorable than the non-microwave assisted sample.

Comparative Study of Herbal Leaves Classification using Hybrid of GLCM-SVM and GLCM-CNN

Indonesia is a tropical country with a diverse range of plants that ancient people used for traditional medicines. However, the similarity in shape of the leaves became an obstacle to distinguishing them. Therefore, technological advancements are expected to help identify the herbal leaves to use them right on target according to their efficacy. In this research, image classification of katuk (Sauropus Androgynus) and kelor (Moringa Oleifera) leaves is applied using 3 different algorithms i.e hybrid of Gray Level Co-Occurrence Matrix (GLCM) feature extraction and Support Vector Machine (SVM) implementing 4 kernels namely linear, RBF, polynomial, and sigmoid; hybrid of GLCM and Convolutional Neural Network (CNN); and pure CNN. A dataset of 480 images has been collected with 2 different scenarios, including bright and dark intensities. Based on the result, a hybrid of GLCM and SVM showed the highest accuracy of 96% in the dark intensity test using a linear kernel, while sigmoid obtained the lowest accuracy of 35%. On the other hand, it has been discovered that CNN obtained the highest performance in the bright intensity test with an accuracy of 98%. While in the dark intensity test, a hybrid of GLCM and CNN is superior, obtaining 96% accuracy. In conclusion, CNN is more powerful for image classification with bright intensity. For dark intensity images, both the hybrid of GLCM+SVM (linear) and the hybrid of GLCM+CNN are fairly recommended.