Differential Code Bias (DCB) is a crucially systematic error in satellite positioning and ionospheric modeling. This study aims to estimate the BeiDou-3 global navigation satellite system (BDS-3) satellite DCBs by using the single-frequency (SF) uncombined Precise Point Positioning (PPP) model. The experiment utilized BDS-3 B1 observations collected from 25 International GNSS Service (IGS) stations located at various latitudes during March 2023. The results reveal that the accuracy of estimating B1I-B3I DCBs derived from single receiver exhibits latitude dependence. Stations in low-latitude regions show considerable variability in the root mean square (RMS) of absolute offsets for satellite DCBs estimation, covering a wide range of values. In contrast, mid- to high-latitude stations demonstrate a more consistent pattern with relatively stable RMS values. Moreover, it has been observed that the stations situated in the Northern Hemisphere display a higher level of consistency in the RMS values when compared to those in the Southern Hemisphere. When incorporating estimates from all 25 stations, the RMS of the absolute offsets in satellite DCBs estimation consistently remained below 0.8 ns. Notably, after excluding 8 low-latitude stations and utilizing data from the remaining 17 stations, the RMS of absolute offsets in satellite DCBs estimation decreased to below 0.63 ns. These enhancements underscore the importance of incorporating a sufficient number of mid- and high-latitude stations to mitigate the effects of ionospheric variability when utilizing SF observations for satellite DCBs estimation.