Sardine Meal Research Articles

Comparative analysis of the chemical quality of fishmeal produced on the Northwest coast of Mexico

Objective: To evaluate the physico-chemical quality of fishmeal produced by four companies in different states of the Republic (Baja California Sur, Jalisco and Sinaloa). Design/methodology/approach: The analyzed fishmeals were from six batches, sardine meal from California pilchard and Pacific thread herring (S. sagax and O. libertate), and skipjack tuna and (K. pelamis) processed by different Mexican companies. Proximal chemical analysis was carried out at the Centro de Investigaciones Biológicas del Noroeste (CIBNOR). Results: The fishmeal’s quality parameters analyzed in this study showed similar values to those reported in the literature. The variations observed in their proximate chemical composition allow them to be classified according to the results of the analyses. Limitations on study/implications: Considering that four of the six flours were produced from the same raw material, S. sagax, the high variability in their physico-chemical quality parameters indicates a lack of standardization in both production methods and quality controls among the producing companies. Findings/conclusions: K. pelamis by-products can produce meals of equal or better physico-chemical quality than those produced from S. sagax. The development of official regulations establishing quality standards to fishmeal production at national level is desirable for competitiveness.

Development and optimization of an extruded aquafeed formulated with moringa flour as a partial substitute for sardine meal

ABSTRACT This study aimed to optimize an extruded aquafeed for Oreochromis niloticus (Nile tilapia) using moringa as a partial protein source. Response surface methodology (RSM) with a Rotatable Central Composite Design (CCRD) was employed to evaluate the four factors influence: sardine meal (28.5–39.25%), moringa flour (34.85–45.60%), temperature (110–140°C) and moisture content (13–17%) on aquafeed’s physicochemical characteristics: expansion index (EI), bulk density, water solubility index (WSI), water absorption index (WAI), hardness and buoyancy. Numerical optimization identified 100 potential diets, of which four were selected and met the criteria for high WSI (11–14%), low hardness (22–32 N) and 100% buoyancy. The best diet consisted of 31.19% sardine meal and 42.91% moringa flour, processed at 132.5°C and 16% moisture, presenting 13.68% WSI, full buoyancy, 29.63 N hardness, 36.33% protein, 10.71% lipids, and 31.36% carbohydrates, applicable to fingerling O. niloticus.

Apparent digestibility coefficients of selected protein ingredients for juvenile Totoaba macdonaldi

AbstractTwo feeding trials were performed to evaluate the apparent digestibility coefficients (ADCs) of dry matter, protein, and amino acids of three animal‐origin and four plant‐origin ingredients in Totoaba macdonaldi. In the first feeding trial, the animal‐origin ingredients were evaluated using totoaba juveniles with an initial weight of 529.7 ± 104.2 g, and for the second feeding trial 745.9 ± 210.6 g. Evaluated ingredients were: poultry by‐product meal, meat and bone meal, feather meal (FM), soy protein concentrate, soybean meal (SBM), corn gluten (CG), and wheat gluten (WG). Each experimental ingredient was evaluated in triplicate. ADCs of dry matter ranged from 35.9% for FM to 67.9% for poultry by‐product, while the protein ADCs values ranged from 41.7% for CG to 83.2% for poultry by‐product. Fish meal ADC (79.6%) was similar to poultry by‐product but significantly higher than WG and soy protein concentrate (72.5% and 72.6%, respectively). The ADCs for lysine were significantly higher for WG, sardine meal, poultry by‐product, and SMB (94.5%, 79.8%, 79.4%, and 79.4%, respectively). Based on the results from the present study, poultry by‐product, WG, and soy protein concentrate are the most promising alternative ingredients (i.e., to the fishmeal) for the formulation of totoaba grow‐out feeds.

<i>Bacillus amyloliquefaciens</i>胞子の乾燥魚粉からの分離と超高温極短時間殺菌装置における熱死滅

<i>Bacillus amyloliquefaciens</i>胞子の乾燥魚粉からの分離と超高温極短時間殺菌装置における熱死滅

Pterygoplichthys spp. (Siluriformes: Loricariidae) meal is suitable for the culture of Nile tilapia Oreochromis niloticus (Cichlidae) juveniles

ABSTRACT: We search for positive utility impact on the invasive species Pterygoplichthys spp. The optimal level of replacement of sardine meal (SM) by Pterygoplichthys spp. meal (PLM) in practical feeds for fry of Nile tilapia (Oreochromis niloticus) was evaluated. We evaluated six experimental diets: 50% PLM-50% SM, 60% PLM-40% SM, 70% PLM-30% SM, 80% PLM-20% SM, 90% PLM-10% SM and 100% PLM-0% SM. In a recirculation system, 270 sex-reversed tilapia fry were used (0.7 ± 0.1 g). Diets were administered in triplicate and the experiment lasted 56 days with sampling every 14 days. There were no statistical differences in growth. At the end of the experiment, the SGR, ADG, CF, WG, DFI, FCR and PER were determined without significant differences (P > 0.05) between treatments, but there was a tendency that could indicated higher WG and PER in the 90% PLM-10% SM treatment (93.56 ± 0.43% and 8.44 ± 1.18 g, respectively). All survival rates were higher than 90% with no significant differences. Additionally, there were no statistical differences in the chemical composition of the whole fish, while apparent digestibility coefficients of protein (81-90%) and lipids (87-93%) also showed no significant differences. We concluded that 100% PLM can replace 100% SM in feeding fry of O. niloticus without affecting their survival, growth or chemical composition or the apparent digestibility of nutrients.

Effect of Soy Protein Products and Gum Inclusion in Feed on Fecal Particle Size Profile of Rainbow Trout

Replacement of fishmeal (FM) with alternative plant proteins, especially soybean meal (SBM), can cause a diarrhea-like symptom in rainbow trout (RBT), characterized by very fine fecal particles. These fines do not settle out in raceway effluent for collection and can contribute to pollution of receiving waters. In this study, two experiments were conducted. Experiment 1 examined effects of nine protein sources (sardine meal, menhaden meal, soy protein concentrates (SPC) (three types), SBM (regular and high protein), corn protein concentrate (CPC), and poultry by-product meal (PBM)) on fecal particle size distribution. Results showed that all five soy-based diets produced feces in RBT having 75.7–89.3% fines and only about 1.0% large particles, while the remaining four diets yielded feces having a balanced particle size distribution. Oligosaccharides present naturally in soy products, thought to contribute fecal fines, were not correlated to fecal particle size classes. Instead, high crude fiber content in soy-based diets was found to be responsible for unbalanced fecal particle distribution in RBT. Experiment 2 examined if improvements in formulation could reduce the negative effect of soy-based ingredients. Eight practical diets (FM, SPC, SPC + 0.3% guar gum, PBM + CPC, PBM + CPC + 20 or 30% SPC, and PBM + CPC + 20 or 30% SPC + 0.3% guar gum) were formulated to contain 40% protein and 20% lipid. Results showed that diets containing mixtures of PBM, CPC, and 20% or 30% SPC plus guar gum produced trout feces with the highest percentage of large particles and lowest of fines, while the diet containing SPC alone (56%) plus guar gum resulted in trout feces having the highest content of mid-size particles. It was concluded that crude fiber in soy protein products (SBM and SPC) caused undesirable fecal particle profiles in RBT, and the addition of guar gum could significantly alleviate this negative effect.

Attractiveness of various crude feed ingredients to juvenile olive flounder (Paralichthys olivaceus, Temminck & Schlegel) and its application to aquaculture

The attractiveness of crude feed ingredients to olive flounder and effects of including feed ingredients showing strong feeding attractiveness in extruded pellet (EP) on performance of fish were determined. Four types of experimental EP were prepared to evaluate the effects of feed ingredient manipulation on performance of fish. Five per cent anchovy meal component of the control diet was substituted with an equal amount of jack mackerel meal, sardine meal and hydrolysed fish meal to create the JM, SM and HFM diets respectively. The diets were prepared in commercial form as EP. Jack mackerel meal produced the strongest feeding attractant responses of olive flounder, followed by sardine meal and hydrolysed fish meal. The greatest weight gain, feed consumption and condition factor (CF) were observed in fish fed the JM diet, followed by fish fed the SM, HFM and control diets, in that order in the 8-week feeding trial. The dietary manipulation of feed ingredients performed in this study showed that the inclusion of ingredients with strong feeding attractiveness improved feed consumption and eventually accelerated the growth performance and CF of the fish.

Apparent digestibility of protein, amino acids and gross energy in rainbow trout fed various feed ingredients with or without protease

Apparent digestibility coefficients (ADCs) of dry matter, crude protein, amino acids, and gross energy in various feed ingredients with or without protease supplementation (Jefo Nutrition Inc., Quebec, Canada) were determined for rainbow trout in a two-year study. The ingredients consisted of two feather meals, two poultry by-product meals, two meat and bone meals, sardine meal, menhaden meal, black soldier fly larvae meal, Methanococcus maripaludis single cell protein, soybean meal, canola meal, distiller's dried grains with solubles (DDGS), cottonseed meal, peanut meal, sunflower meal, and algae (Spirulina sp.) meal. A reference diet with 0.1% yttrium oxide as inert marker was mixed with test ingredients in a 70:30 ratio to produce a series of test diets. Fish were fed their respective diets and fecal samples were collected by stripping. ADC of dry matter for rainbow trout ranged 51.0–86.6% for animal products and single cell protein and 33.1–70.1% for plant products without protease supplementation. ADC (without protease supplementation) of protein and energy ranged from 55.4–84.5% and 58.1–90.2%, respectively, for animal products, and 70.0–83.8% and 32.9–76.0%, respectively, for plant products. Supplementation with the commercial protease (175 mg protease complex/kg of diet) resulted in ingredient-specific ADC increases for dry matter, energy, cysteine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tyrosine, alanine, aspartic acid and glutamic acid, with most ingredients having improved digestibility of at least one amino acid. Protease supplementation had the most profound improvement on ADCs for soybean meal, including dry matter and the majority of individual amino acids. Overall, this research demonstrates the benefit of protease supplementation on the digestibility of feed ingredients commonly used in rainbow trout and other commercially cultured fish feeds, although the degree of improvement in digestibility varied among ingredients. Further studies with this protease are recommended to evaluate its effects at higher inclusion levels and in other species of cultured fishes.

<b>Formulation of fish waste meal for human nutrition

This study aimed to elaborate and characterize meals containing waste from processing of tilapia, tuna, salmon and sardine for human consumption. Carcasses of tilapia and salmon, tuna torsos without fins and sardine tails were cooked, pressed, milled and dehydrated, resulting in waste meal. Greater protein (83.28%) and lower mineral matter (5.31%) were observed in tuna meal. Salmon meal presented greater content of lipids (18.81%) and sardine meal, lower content (3.98%). Tilapia meal presented greater mineral matter (37.66%), calcium (9.37%) and phosphorus (6.08%). Higher content of iron was observed in sardine and tuna meals. Higher amounts of fatty acids from n-3 series were found in salmon (53.71 g kg-1), sardine (47.46 g kg-1) and tuna (36.98 g kg-1). Concerning amino acids, glutamic acid showed greater proportion in all meals, followed by lysine, leucine, glycine and aspartic acid. All meals presented high biological and nutritional values and are regarded as important sources of calcium, phosphorus and iron.

Amino acid digestibility of animal protein ingredients for bullfrogs in different phases of post-metamorphic development

The development of diets that economically and ecologically meet the requirements of bullfrogs (Lithobates catesbeianus) is important for frog farming. Therefore, the objective of this study was to determine the apparent digestibility coefficients for essential amino acids of eight animal feed ingredients (poultry byproduct meal, meat and bone meal, salmon byproduct meal, tilapia byproduct meal, spray-dried blood meal, powered red blood cells, feather meal and sardine meal) used in the diets of bullfrogs. Additionally, the limiting digestible amino acids were determined for each ingredient. Bullfrogs in different phases of post-metamorphic development were used: 810 animals in the early phase (mean weight: 40 ± 10 g), 405 animals in the growing phase (95 ± 15 g) and 243 animals in the finishing phase (175 ± 25 g). Poultry byproduct meal, salmon byproduct meal and blood meal exhibited the largest number of high apparent digestibility coefficients for essential amino acids (>70%) in the early, growing and finishing phases, respectively. The animal protein ingredients were well digested by the bullfrogs and could be used in practical diets for this species, respecting the limits of inclusion and digestibility values for each developmental phase.

Extruded snacks with the addition of different fish meals

Tilapia, salmon, tuna and sardine meals were prepared to develop and analyze extruded snacks with residue meal from fish processing. Residue meals were included in five types of corn snacks: control (0% fish meal) and four with 9% tilapia, salmon, tuna and sardine meals. Although moisture, lipids and carbohydrates rates did not differ among the snacks, protein rates increased with the increment of fish meal, reaching 11.85% in the tuna snack. Tuna and sardine snacks had the highest iron levels. The most abundant fatty acids were linoleic, oleic, palmitic, linolenic and stearic acids, with sardine, salmon and tuna snacks presenting the highest values of n-3 series fatty acids. Greater luminosity rate was reported for salmon snack, followed by tilapia, tuna and sardine snacks. The highest sensory acceptance index was verified in tilapia (78.07%) and salmon (72.40%). A 9% addition of residue meals of tilapia, salmon and tuna improved the nutritional value of the snacks.

Digestibility of selected feedstuffs in subadult Caspian great sturgeon,Huso husousing settlement faecal collection and stripping methods

Apparent crude protein (ADCCP), crude fat (ADCCF) and gross energy (ADCGE) digestibility coefficients of several typical and novel feedstuffs were determined to be employed in the formulated diets of subadult beluga (17.70 ± 0.234 kg) diets via two faecal collection methods of settlement (SFCM) and stripping (StM). The tested feedstuffs included three fish meals, three terrestrial animal by-products, three plant protein concentrates, nine high-protein plant meals and three low plant protein meals. ADCCP values varied in different fish meals ranging from 89.5% for Mexican sardine meal to 93.2% for anchovy meal with SFCM. ADCCP values for plant protein concentrates ranged from 82.8% for wheat gluten meal to 92% for soy protein with SFCM. ADCCP ranged from 37.2% for whole wheat to 75.6% for spirulina meal with SFCM. ADCGE was lower in low-protein plant meals (64.1%) in comparison to high-protein plant meals (70.2%). Fish meals showed the highest ADCCF values. A significant (P < 0.05) quadratic regression relation (r2 = 0.92) was observed among in vivo ADCCP of five feedstuff classes fed to beluga and in vitro ADCCP. The differences among ADC values appear to be significantly related to the effects of feedstuffs and therefore diet and faecal collection methods.

Quantifying energy intake in Pacific bluefin tuna (Thunnus orientalis) using the heat increment of feeding

Using implanted archival tags, we examined the effects of meal caloric value, food type (sardine or squid) and ambient temperature on the magnitude and duration of the heat increment of feeding in three captive juvenile Pacific bluefin tuna. The objective of our study was to develop a model that can be used to estimate energy intake in wild fish of similar body mass. Both the magnitude and duration of the heat increment of feeding (measured by visceral warming) showed a strong positive correlation with the caloric value of the ingested meal. Controlling for meal caloric value, the extent of visceral warming was significantly greater at lower ambient temperature. The extent of visceral warming was also significantly higher for squid meals compared with sardine meals. By using a hierarchical Bayesian model to analyze our data and treating individuals as random effects, we demonstrate how increases in visceral temperature can be used to estimate the energy intake of wild Pacific bluefin tuna of similar body mass to the individuals used in our study.

Availability of squid viscera meal with cadmium removal treatment as a feed ingredient for fingerling black rockfish Sebastes schlegeli

Two 12-week feeding experiments were conducted to evaluate the nutritional value of squid viscera meal with cadmium removal treatment (dCSVM), which contained 1.5–2.0 mg/kg cadmium, as an alternative protein source to sardine meal in diets for fingerling black rockfish Sebastes schlegeli. Initial mean body weights in experiments 1 and 2 were 23.0 and 6.4 g, and the replacement rates of sardine meal with dCSVM in the test diets were 20–80 % and 30–60 %, respectively. In experiment 1, inclusion of dCSVM at 40 % and higher replacement rates of sardine meal retarded the growth of fish due partly to inferior dCSVM protein digestibility. The cadmium (Cd) concentrations in fish muscle of all treatment groups were below the detection limit (<0.1 mg/dry matter). In experiment 2 using dCSVM containing Cd at a lower level and having a higher protein digestibility than in experiment 1, no significant differences were observed in the growth between the control and 60 % replacement groups, although feed efficiency gradually decreased with the increase of dCSVM inclusion. These results show that dCSVM is safe and useful as an alternative protein source and could replace up to 60 % of sardine meal in fingerling black rockfish diets.

Altered Growth Rates, Carcass Fatty Acid Concentrations, and Tissue Histology in First‐Feeding Steelhead Fed a Fish‐Meal‐ and Fish‐Oil‐Free Diet

AbstractA 12‐week feeding trial was conducted to evaluate a fish‐meal‐free, fish‐oil‐free diet for use with first‐feeding steelhead Oncorhynchus mykiss. The marine‐based control diet (marine diet) contained sardine (Sardinops spp.) meal and pollock (Pollachius virens) liver oil as the primary sources of protein and lipid, respectively. The experimental diet (terrestrial diet) contained only terrestrial sources of protein (poultry by‐product meal, blood meal, canola, corn gluten, and wheat gluten) and lipid (canola oil and flaxseed oil). Each diet was administered to five replicate groups of first‐feeding fry. At the conclusion of the feeding trial, fish that were fed the marine diet exhibited significantly higher weight gain and feed efficiency than fish that were given the terrestrial diet. Survival was not significantly affected by dietary treatment. However, fish that received the terrestrial diet exhibited significantly lower hemoglobin concentration and percent packed cell volume. Histological examination indicated that in comparison with control fish, steelhead that were fed the terrestrial diet exhibited a lesser degree of vacuolation in hepatocytes, mild hypertrophy and hyperplasia of the nephron tubular epithelium, and intracellular vacuolation in epithelium of the pyloric caeca. Carcass crude protein concentration was significantly higher in fish that were fed the marine diet than in fish that were fed the terrestrial diet. After 2 weeks of feeding, steelhead that received the marine diet exhibited significantly higher carcass concentrations of 14:0, 16:0, 16:1, 20:1, 20:5(n‐3), 22:5(n‐3), and 22:6(n‐3) fatty acids but significantly lower carcass concentrations of 18:1, 18:2(n‐6), 18:3(n‐6), 18:3(n‐3), and 20:4(n‐6) relative to fish that were given the terrestrial diet. These differences in carcass fatty acid concentrations persisted throughout the feeding trial. Results of this study indicate that the current terrestrial‐based diet is not a satisfactory replacement for the standard marine‐based diet used with first‐feeding steelhead.Received January 21, 2010; accepted January 22, 2011

In vitro amino acid absorption using hydrolysed sardine muscle or soybean meal at different intestinal regions of the Pacific bluefin tuna (Thunnus orientalis)

The amino acid (AA) absorption along the intestinal tract of the Pacific bluefin tuna (Thunnus orientalis) was evaluated using two hydrolysed protein sources (fresh sardine muscle and soybean meal) with the everted intestine technique. Pork pepsin and pancreatic enzyme extract from the bluefin tuna were used to hydrolyse the protein from fresh sardine (FSH) and soybean meal (SMH) under optimal bluefin tuna fish physiological conditions. Both of the hydrolysate solutions were tested within three intestinal sections from the bluefin tuna. The everted intestinal fractions immersed in the hydrolysate solutions were sampled at different times to analyse for AA and absorption rate calculations. Fresh sardine and SMH contained greater amounts of essential amino acids (EAA) than those of non-essential amino acids (NEAA); however, the profiles of AA absorbed showed higher absorption of NEAA in both cases. Using a similar concentration solution, the absorption rates within the intestinal fractions showed a preferential absorption in the proximal and distal regions for Arg and His when FSH was used. However, the absorption rates for Lys resulted in a decreasing proximal-to-distal gradient between the different intestinal regions for FSH and SMH. The possibility of a catabolic role of certain AAs in the enterocytes being able to explain the differences in absorption is discussed.

Evaluating the Effects of Formulated Moist Diets on Juveniles of Patagonian OctopusEnteroctopus megalocyathus(Gould 1852)

The aim of this study was to evaluate the performance of Patagonian octopus fed with moist diets formulated with several local feed ingredients. All formulated diets were based on crab paste (70%) and the experimental feed ingredient (30%). Experiment 1 assayed salmon meal, prime sardine meal, and wheat gluten, using fresh fish as a control; experiment 2 assayed prime fish meal and macroalgal meal against crab paste alone as a control. The ingestion rate was lower than expected for all diets except those of fresh fish, crab paste alone, and crab paste plus prime sardine meal. No significant differences were found in the observed digestibility of the diets, indicating, in general, low digestibility, even for fresh fish. The highest protease values were observed for crab paste plus prime sardine meal in both experiments. The better growth of Enteroctopus. megalocyathus was obtained when these were fed fresh fish, which was associated with the greater consumption observed in this diet, as neither the digestibility nor the enzymatic activities of the hepatopancreas were related to this greater growth.

사료원료에 대한 넙치 Paralichthys olivaceus 소화율 평가

The apparent digestibility coefficients of dry matter, crude protein and energy in white fishmeal, herring meal, anchovy meal, salmon meal, sardine meal, mackerel meal, squid meal, soybean meal, corn gluten meal and wheat flour were determined for olive flounder. Digestibility coefficients were determined using a reference diet and test diets that contained 70% of the reference diet mixture and 30% test ingredients. All diets contained 0.5% chromic oxide as a digestibility indicator. The fish averaging 220 g were held in 500 L tanks at a density of 20 fish per tank. Feces were collected from three replicated groups of fish using a fecal collection column attached to a fish-rearing tank. The apparent dry matter, crude protein and energy digestibility coefficient values observed were in the ranges 29-79%, 59-95% and 45-91%, respectively, for various test ingredients. The apparent dry matter, crude protein and energy digestibility of white fishmeal, herring meal, anchovy meal, salmon meal, sardine meal, mackerel meal and squid meal were significantly higher than those of soybean meal, corn gluten meal and wheat flour.

Carbohydrate and lipid digestibility of animal and vegetal ingredients and diets for the pre-adult redclaw crayfish, Cherax quadricarinatus (von Martens)

In vivo carbohydrate and lipid digestibility of four animal [sardine meals 67% and 58% crude protein (CP), squid meal and red crab meal] and three vegetal (soy paste, textured wheat and sorghum meal) ingredients and diets were evaluated in experimental diets for pre‐adult redclaw crayfish, Cherax quadricarinatus. A reference and seven experimental diets were formulated, including 14.5% of each ingredient in the reference diet. A single factor, completely randomized experimental design with five replicates per treatment was done. Experimental organisms were pre‐adult redclaw of 10±0.8 g. The best carbohydrate digestibilities were recorded for some vegetal ingredients such as sorghum and textured wheat (93.6% and 83.9%) and the corresponding diets (90.9% and 90.8%). For animal ingredients, carbohydrate digestibilities were very poor (from 12.3% to 41.1%), and the inclusion of these ingredients diminished the digestibility of the corresponding experimental diets as compared with the reference diet (86.3–89.2% vs. 89.9%). The highest lipid digestibility corresponded to red crab meal (97.5%). Textured wheat and soy paste meal also had excellent lipid digestibility (96.4% and 95.1%). The same tendency was found for the corresponding diets. The lowest percentages were obtained with 58% crude protein sardine meal (32.9%) and its diet (86.2%).

Effect of Microparticulate Diets on Growth and Survival of Spotted Sand Bass Larvae, Paralabrax maculatofasciatus, at Two Early Weaning Times

Abstract Early weaning in spotted sand bass larvae, Paralabrax maculatofasciatus, was evaluated, testing a combination of two weaning times, 17 and 22 d after hatching (d.a.h.), and three different microparticulate diets. Protein in diets was mainly from sardine meal and from 15% squid meal, beef blood meal, or fish protein hydrolysate. Anatomical (standard length), histological (gut development), and biochemical (highly unsaturated fatty acids) parameters were measured in larvae, as well as survival and resistance to a stress test measured 40 d.a.h. For larvae weaned at 17 d.a.h., the best growth and survival were obtained with diets containing fish protein hydrolysates; for larvae weaned at 22 d.a.h., best results were obtained with squid meal and fish protein hydrolysate. Growth and survival were significantly lower when using beef blood meal in both weaning treatments. The best relative and total survival were for larvae weaned at 22 d.a.h. After the resistance test, 100% survival occurred in larvae fed on any microparticulate diet and either weaning treatment. No significant differences in arachidonic acid, eicosapentaenoic acid, or docosahexaenoic acid concentrations in fish fed on any diet occurred. Results suggest that weaning at 22 d.a.h. with diets containing fish protein hydrolysate or squid meal is preferred by this species.