Background: Lolly ice cream is a frozen sweetened product made of heat treated mix, with or without other ingredients and permitted additives. The objectives of this study were to develop lychee flavored lolly ice cream, to determine the overall acceptance of lychee flavored ice cream (sensory evaluation) and to use accelerated shelf-life testing to study the changes in acidity, total soluble solid (TSS), total solid, microbiology, and sensory characteristics of lolly ice cream. Methods: The physical and chemical characteristics and the acceptance of lychee lolly ice cream flavored with lychee were evaluated and compared with lemon and orange lolly ice cream. A testing panel consisting 16 panelists studied the acceptability of the sample based on a 9-point hedonic scale. The consumer’s preferences were measured by statistical analysis of the scores obtained from the response of the panel. Result: Lychee flavored lolly ice cream showed overall acceptance than orange and lemon lolly ice cream. Our results suggest that the shelf life of lolly ice cream sample was 1 year at -20°C and set expiration date on ice cream might also contribute to effective management of ice cream characteristics in the retail chilled chain.