Samples Of Seeds Research Articles

Heterogeneity in Seed Samples from Vineyards and Natural Habitats Along the Eurasian Vitis vinifera Range: Implications for Domestication and Hybridization

By exploring seed samples from vineyards and natural habitats across the Eurasian range of Vitis vinifera, our analysis revealed substantial morphological variation within populations. Through the analysis of domestication index values, probabilities, and entropy, we assessed seed diversity. Samples with high domestication probability values—predominantly from vineyards—exhibited low heterogeneity and entropy, with similar patterns observed in natural habitats, suggesting the presence of feral vines. In parallel, seeds with low domestication index values, found mainly in natural habitats, also displayed low entropy and are likely associated with Vitis sylvestris or other wild Vitaceae species. Intermediate domestication values pointed to hybrid swarms, highlighting the crucial role of hybridization in the development of modern grapevine cultivars. The study identified mixed populations across the Iberian Peninsula, Italy, and the South Caucasus, which act as significant gene reservoirs. A domestication gradient is evident, with higher domestication rates in the South Caucasus compared to Western Europe and East Asia. The results demonstrate the significance of these mixed populations as repositories of genetic diversity, underscoring their conservation value, particularly considering the negative impact of habitat alterations, especially in riparian forests due to major public works.

CHARACTERIZATION OF SOME MAIZE HYBRIDS BASED ON HECTOLITER WEIGHT

The study analyzed a collection of 20 corn hybrids based on the hectoliter weight of the grains. The hybrids were tested in culture within ARDS Lovrin, in the 2023-2024 agricultural year. The biological material came from ARDS Suceava. In the case of the experiment, the hybrids were marked SV1 to SV20. The experimental variants, given by corn hybrids, were cultivated in repetitions. Adequate crop technology was provided to ensure uniform conditions for corn hybrids. Seed samples were taken from each hybrid, on repetitions for analysis. The values ​​of the HW parameter varied between HW = 71.55±0.68 kg hl-1 (SV2) and HW = 83.70±0.68 kg hl-1 (SV7, SV18). The series of experimental data showed normal distribution, with r = 0.976. Anova Test confirmed the safety of the data and the presence of variance in the data set (p = 0.000662; F > Fcrit). The mean value calculated at the level of the experiment was HW_m = 77.448 kg hl-1. Compared to the mean value, 11 hybrids showed values ​​above the average, and nine hybrids showed values ​​below the mean. Values ​​above the mean were presented by hybrids SV7, SV15 and SV18 (p<0.001), and hybrid SV13 (p<0.05). Values ​​below the mean were presented by SV2, SV12, and SV19 hybrids (p<0.001), SV6 hybrid (p<0.01), and SV1 and SV10 hybrids (p<0.05). The cluster analysis led to the grouping of hybrids based on similarity (Coph.corr. = 0.833). In relation to the thresholds of the quartiles, the hybrids SV2, SV19, SV12, SV6, SV10 were included in quartile 1. The SV1, SV5, SV16, SV14, SV11, SV8, SV20, SV9, SV17, SV3 hybrids were included in the 2nd and 3rd quartiles. In the 4th quartile were the hybrids SV4, SV13, SV15, SV7, SV18.

Differentiation of Soybean Genotypes Concerning Seed Physiological Quality Using Hyperspectral Bands

The use of summarized spectral data in bands obtained by hyperspectral sensors can make it possible to obtain biochemical information about seeds and, thus, relate the results to seed viability and vigor. Thus, the hypothesis of this work is based on the possibility of obtaining information about the physiological quality of seeds through hyperspectral bands and distinguishing seed lots regarding their quality through wavelengths. The objective was then to evaluate the possibility of differentiating soybean genotypes regarding the physiological quality of seeds using spectral data. The experiment was conducted during the 2021/2022 harvest at the Federal University of Mato Grosso do Sul in a randomized block design with four replicates and 10 F3 soybean populations (G1, G8, G12, G15, G19, G21, G24, G27, G31, and G36). After the maturation of each genotype, seeds were harvested from the central rows of each plot, which consisted of five one-meter rows. Seed samples from each experimental unit were placed in a Petri dish to collect spectral data. Readings were performed in the laboratory at a temperature of 26 °C and using two 60 W halogen lamps as the light source, positioned 15 cm between the sensor and the sample. The sensor used was the Ocean Optics (Florida, USA) model STS-VIS-L-50-400-SMA, which captured the reflectance of the seed sample at wavelengths between 450 and 824 nm. After readings from the hyperspectral sensor, the seeds were subjected to tests for water content, germination, first germination count, electrical conductivity, and tetrazolium. The data obtained were subjected to an analysis of variance and the means were compared by the Scott–Knott test at 5% probability, analyzed using R software version 4.2.3 (Auckland, New Zealand). The data on the physiological quality of the seeds of the soybean genotypes were subjected to principal component analysis (PCA) and associated with the K-means algorithm to form groups according to the similarity and distinction between the genetic materials. After the formation of these groups, spectral curve graphs were constructed for each soybean genotype and for the groups that were formed. The physiological quality of the soybean genotypes can be differentiated using hyperspectral bands. The spectral bands, therefore, provide important information about the physiological quality of soybean seeds. Through the use of hyperspectral sensors and the observation of specific bands, it is possible to differentiate genotypes in terms of seed quality, complementing and/or replacing traditional tests in a fast, accurate, and non-destructive way, reducing the time and investment spent on obtaining information on seed viability and vigor. The results found in this study are promising, and further research is needed in future studies with other species and genotypes. The interval between 450 and 649 nm was the main spectrum band that contributed to the differentiation between soybean genotypes of superior and inferior physiological quality.

Mongolian accessions of crested wheatgrass (Agropyron cristatum (L.) Beauv.) in the VIR collection

Recently, the interest in forage crops with resistance to adverse environmental factors in changing climate conditions has grown significantly among researchers. Among these crops is wheatgrass, diploid specimens of which are included as the initial genetic material in breeding and genetic programs. The first and only one wild specimen of crested wheatgrass was collected in 1922 by V.E. Pisarev, a close associate of N.I. Vavilov, during the Mongolian expedition of the State Institute of Experimental Agronomy. Two more wheatgrass specimens collected by A.I. Ivanov, the Leading Researcher of the Department of Forage Crops of VIR, arrived in 1966.It was only in 1986-1987 that the VIR collection was replenished with a rather large number of diploid wild-growing Mongolian specimens of crested wheatgrass, distinguished by traits valuable for breeding, such as short vegetation period, salt-, drought-, and winter hardiness, resistance to diseases and pests.In the summer of 1986 and from the end of summer until the autumn of 1987, scientists from the Plant Resources Team of the Joint Soviet-Mongolian Complex Biological Expedition (JSMKBE) examined the flora of several aimags of Mongolia: Selenge, Bulgan and Central aimags in 1986; Arkhangai, Bayankhongor, Bulgan, East Gobi, Selenge, Uvs, Arkhangai, Khentii, Central and South Gobi aimags in 1987.The objective of the Plant Resources Team was to broaden the gene pool of crop wild relatives (CWR). Seed samples of mid- and late-ripening perennial wild forage grasses resistant to limiting environmental factors, were collected for replenishing plant collections of the USSR and Mongolia, study, and further use by breeding centers of both countries. During the expedition, the institute’s collection was replenished with samples of forage grasses, including 30 of crested wheatgrass, one of Mongolian wheatgrass, Michno's wheatgrass and Ericksson crested wheatgrass. The geographical coordinates and collection sites of the samples were recorded, and the ecological and a geographical classification of the Mongolian populations of crested wheatgrass was created.

Alternative hosts of banana bunchy top virus in the Philippines and the first evidence of seed transmission of BBTV.

Banana bunchy top disease is caused by banana bunchy top virus (BBTV). BBTV is transmitted locally by aphids (Pentalonia spp.), but the long-distance spread is through the movement of infected planting materials. This study investigated potential alternative hosts of BBTV in ornamental Musa and related species in the Zingiberales in the Philippines. Artificial inoculation of BBTV, molecular detection and transmission assay were used to evaluate 15 plant test species. The potential for seed transmission of BBTV through Canna indica seeds was also investigated. Seed samples were validated and quantified for BBTV presence using molecular tools, and then grown for transmission assay. Typical symptoms of BBTV in bananas, including dark green streak on the midrib and petiole and rosetting were observed on inoculated Musa coccinea (banana blossom), M. velutina (velutina), M laterita. (bronze banana) and Canna indica (Bandera Espanola). PCR assays confirmed BBTV infection in these symptomatic test plants, as well as in Curcuma longa (turmeric) which exhibited large chlorotic blotches on the leaf. BBTV was detected from both seeds and germinated seedlings of artificially inoculated and field-collected C. indica samples. This study identified M. laterita as a new host of BBTV. The susceptibility to BBTV of M. coccinea, M. velutina, C. indica, and C. longa was also confirmed. The study also provided the first evidence of seed transmission of BBTV. C indica is an ornamental plant popularly used for landscaping in the Philippines and seeds were shown to be an efficient mode of transmission of the virus with rates up to 34%. The discovery of natural infection in ornamental plants and seeds poses a risk to the banana industry and responsible propagation and appropriate quarantine protocols must be implemented.

Phenological and Fungal Interactions of Malesherbia auristipulata Ricardi (Passifloraceae) in the Atacama Desert: Adaptations and Conservation in an Extreme Ecosystem.

Malesherbia auristipulata is an endemic plant species of the Atacama Desert, with unique morphological and physiological adaptations. This research was conducted at Cuesta El Águila, Arica and Parinacota Region, Chile. Adult and juvenile plants were monitored, recording their growth, flowering, and fruiting phases. Additionally, plant community species were identified. For the study of endophytic mycoflora, samples of seeds, roots, stems, and leaves were collected, disinfected, and cultivated in specific media. The isolated fungi were analyzed morphologically and molecularly, determining their distribution in different plant organs. The diversity of endophytic fungi associated with M. auristipulata and the associated fungal community was determined. The presence of endophytic fungi varied depending on the organ studied, suggesting dynamic interactions in the structure of its fungal community. Among the identified endophytic fungi, Alternaria sorghi, A. alstroemeriae, and Fusarium nurragi stand out for their presence in the root and stem of the plant. Of particular interest is the presence of F. circinatum in the leaves. This study provides valuable information for the conservation of M. auristipulata and other organisms in the Atacama Desert, highlighting the importance of ecological interactions in the resilience of plants to extreme environmental conditions.

Detection of damage caused by Nezara viridula on soybean using novel imaging approaches based on computed tomography and image color analysis.

Soybean (Glycine max L.) is an important leguminous plant, in which pests trigger significant damage every year. Important members of this community are insects with piercing-sucking mouthpart, especially the southern green stinkbug, Nezara viridula L.. This insect with its extraoral digestion causes visible alterations (morphological and color changes) in the seeds. We aimed to obtain precise information about the extent and nature of damage in soybeans caused by N. viridula using nondestructive imaging methods. Two infestation conditions were applied: one with controlled numbers of pests (six insects/15 pods) and another with naturally occurring pests (samples collected from the apical part of the plant and samples from whole plants). An intact control group was also included, resulting in four treatment groups. Seed samples were analyzed by computed tomography (CT) and image color analysis under laboratory conditions. According to our CT findings, the damage caused by N. viridula changed the radiodensity, volume, and shape (Solidity) of the soybean seeds during the pod-filling and maturing period. Radiodensity was significantly reduced in all three damaged categories compared to the intact sample; the mean radiodensity reduction range was 49-412 HU. The seed volume also decreased significantly (25%-80% decrease), with a threefold reduction for samples exposed to regulated damage compared to natural ones. The samples exposed to natural damage showed significant but minor reduction in solidity, while samples exposed to regulated damage showed a prominent decrease (~12%). Image color analysis showed that the damaged samples were well distinguishable, and the differences were statistically verifiable. The achieved data derived from our external and internal imaging approaches contribute to a better understanding of the internal chemical processes, and CT analysis helps to understand the alteration trends of the hidden structure of seeds caused by a pest. Our results can contribute to the development of a practically applicable system based on image analysis, which can identify lots damaged by insects.

Toxicological evaluation of anti-cough herbal tea made from aqueous extracts of G. Kola, C. Citratus and B. pinnatum

Background: Herbal formulations currently contribute significantly to medical remedies with varying effects on health indices. Garcinia kola seeds, Cymbapogon citratus, and Bryophyllum pinnantum leaves are among the widely used in herbal formulations. Objectives: This study evaluated the toxicological effects of the ternary combination of these plant materials on some renal, hepatic, hematological and histological parameters of streptococci-infected white albino rats. Materials and Methods: Samples of Garcinia kola seeds, Cymbapogon citratus leaves, and Bryophyllum pinnantum leaves were sourced locally and authenticated by a taxonomist. Aqueous extract of the ternary combination comprising of 40% G. kola, 30% C. citratus, and 30% B. pinnantum was prepared and administered on albino rats. Thirty five white albino rats grouped into 7 groups comprising of normal control, treatment control, disease control, and test group were used for the evaluation after a 14 days treatment on infected and uninfected rats. The rats were sacrificed, and samples collected. Some renal, hepatic, histological, and hematological indices were analyzed. Results: Comparison of all hematological parameters (except for platelet count) between uninfected-untreated and unifected-treated with extract showed no significant (p>0.05) difference. Platelet count was significantly (p<0.05) raised in all groups treated with the extract. Comparison control and the extract-treated group showed no significant (p>0.05) difference in renal parameters indicating no adverse effects on renal function. However, comparison of parameters between control and the infected-treated group showed significantly elevated creatinine which alone may not be sufficient to support renal damage. Albumin, and total bilirubin were significantly (p<0.05) lower in the extract-treated group compared to the control group. In contrast, total protein and globulin were significantly (p<0.05) higher in the controls to the infected groups. ALT and AST were significantly (p<0.05) lower in the extract treated groups compared to the control group. Although comparison of ALP activity between uninfected-untreated and infected-treated group showed significant (p<0.05) elevation this alone may not be sufficient to support liver damage. The hematoxyline and eosine stained sections of the kidney and the liver tissues of the tea fed groups show normal kidney and liver histology. There was no significant change in the liver and kidney histology of the test and control groups. The extract did not cause adverse impact on these organs. Conclusion: Ternary combination of 40% G. kola, 30% C. citratus, and 30% B. pinnantum can effectively be used in treatment of cough without significant adverse effects on renal, hepatic, hematological and histological parameters. The ternary combination rather demonstrated significant hepato-protective feature.

Evaluating the Impact of UVC Exposure on Forage Maize Seeds under Hydroponic Condition

Background: UV radiation, with shorter wavelengths than visible light, plays a pivotal role in stimulating growth processes and boosting the synthesis of beneficial compounds in plants. Moreover, it triggers morphological changes and genetic responses, shaping plants’ physical traits and adaptability. Nevertheless, a nuanced understanding is necessary as excessive UV exposure can also cause harm, underscoring the complexity of the relationship between UV radiation and plants. The study aimed to evaluate the effect of ultraviolet C (UVC) light exposure on the germination and growth of forage maize seeds under controlled conditions in an indoor crop. One sample of seeds was exposed to UVC light, while the other was not. Methods: The experiment was carried out for 14 days, with the plants subjected to mixed blue-red light, fertigation and kept at a maximum external temperature of 30°C and maximum internal temperature of 25.1°C. UVC light is applied for 10 minutes to the seeds before germination. Result: The results showed that the plants exposed to UVC light had an average growth increase of 3cm compared to those that did not receive the treatment. However, the protein content of both sets of seedlings was similar. The study suggests that further research is necessary to determine the optimal duration and distance for UVC exposure to minimize any potential negative effects on the plants. It also highlights the need to investigate the effect of UVC radiation on the nutritional content of hydroponic crops to ensure that the increased growth does not come at the expense of nutritional quality. The experiment was carried out in Medellin, Colombia, with a maximum solar radiation of 1000 W/m2 and an altitude of 1500 meters. Overall, the study provides evidence that UVC radiation can enhance the growth of hydroponic maize seedlings, but more research is needed to fully understand its effects. At the end, there is an improvement in the growth of forage corn in seeds that have been subjected to UVC light compared to those that have not. It is worth highlighting the need for more research on changes in nutritional components in general.

Electrochemical sensor for quick and binder-free detection of vitamin B7 using shock waves treated hydroxyapatite/graphene oxide nanocomposite

We report the fabrication of a novel electrochemical sensor for rapid and accurate determination of Vitamin B7 (biotin) using shockwaves treated hydroxyapatite/graphene oxide (SW-HAP/GO) composite modified glassy carbon electrode. Square wave voltammetry measurements at SW-HAP/GO modified GCE exhibits a strong peak at 1.45 V corresponding to the oxidation potential of biotin in phosphate buffer saline at pH 7.0. The results revealed a systematic increase in oxidation peak current with the increase in biotin concentration over a wide range of 16.7–611 µM and the lowest detection limit of 0.215 µM has been deduced. The practicability of the developed sensor has been demonstrated by determining biotin in vitamin B7 tablets, farm fresh groundnuts, dried groundnuts, almonds, pumpkin seeds, and egg yolk. The nuts and seeds samples (10 g each) were soaked in demineralised water for about 10 hours at room temperature and the extracts were used directly for SWV measurements. In all the cases, the SWV revealed a broad peak at about 1.4 V indicating the presence of biotin and from the calibration curves, the actual amount of B7 dissolved in water have been estimated as 1.46, 1.18, 0.43, 4.2, and 5.1 mcg in 10 g of farm fresh groundnuts, dried groundnuts, almonds, pumpkin seeds and egg yolk. Among the investigated samples, pumpkin seeds contain higher amount of biotin and it remained intact even after boiling in water. The fabricated SW-HAP/GO sensor offers potential for quick and precise determination of biotin in food and pharmaceutical industries without requiring streptavidin or antibody.

Combined vigour tests to estimate relative seedling emergence in the field and germination after storage in sweet corn seed lots

This study was carried out to test whether the radicle emergence (RE), cold (CT) and accelerated ageing (AAT) tests and their combinations of CT+RE and AAT+RE can be used to rank sweet corn seed lots according to field seedling emergence and germination after storage. Seed samples for eight hybrid seed lots were stored hermetically over 12 months at 20°C with 9.9-10.2% seed moisture. RE was counted between 32 and 72 hours, AAT was carried out at 41 ± 1°C and 100% RH for 72 hours, and CT at 5°C over two days. RE count was performed after AAT (AAT+RE) and CT (CT+RE). Seedling emergence was tested in two field sowings, in April and May 2022. The vigour test results were correlated with both seedling emergence percentages in the field and germination after storage. Both normal germination after CT (r = 0.73−0.94, P < 0.05−0.001) and AAT+RE72h (r = 0.71−0.84, P < 0.05−0.01) were significantly related to both parameters. The RE test was significantly related to seedling emergence, but not to any post-storage data. The normal seed germination percentage was found to be significant in only one case (sowing 2). Seed vigour in sweet corn seeds and its importance in relation to seedling emergence and seed storage are discussed.

Evaluation of Physiological and biochemical traits of selected soybean line in Makurdi

Twelve genotypes of soybean were selected for physiological and seed biochemical evaluations at Joseph Sarwuan Tarka University, Makurdi. These seeds were collected from NCRI Badegi; Niger State- Nigeria. Ten of these genotypes S1075-6-93, S1179-6-58, S1239-6-135, S1240-6-288, S1252-6-277, S1252-6-156, S1268-6-15, S1273-6-65, S1365-6L-20 and SC SENTINEL is newly introduced to Makurdi while SC SIGNA and SLO1 used as checks have been released. The seed crop field was laid out in a Randomized Complete Block Design (RCBD) with three replications in 4-rows of 5m long. Seed samples were collected and subjected to seed quality tests at the Department of Plant Breeding and Seed Science Laboratory of the University. Data were collected on germination count which was expressed as germination percentage and its (germination rate and germination rate index) were consequentially obtained. These seeds were also subjected to proximate analysis and percentage protein, fat, ash, moisture content and crude fibre were determined using soxhlet method. The result showed that significant differences existed for germination rate index (GRI) and germination index (GI) while other seedling traits were not different in magnitude from each other. Protein was highest in three varieties (S1075-6-93, S1239-6-135 and S1252-6-156) with 40.25%, respectively and significantly different from others and the checks. SC SENTINEL had the least protein content (37.19) and highest oil content (20.82). Meanwhile, genotype S1239-6-135 had the least oil content (18.83). Compositionally, an inverse relationship existed between protein oil in genotypes studied. Biochemical composition has more effect on seed quality of these soybean genotypes than physiological traits. Genotypes (S1075-6-93, S1239-6-135 and S1252-6-156) had higher protein content than the checks. Also, Sc SENTINEL, S1365-6L-20, S1273-6-65, S1268-6-15 and S1240-6-288 had higher oil content than the checks. Soybean oil producers could use SC SENTINEL for their productions and (S1075-6-93, S1239-6-135 and S1252-6-156) could be used for protein-based products.

Physiochemical and Microbial Quality of Melon and Bush Mango Seeds and Milled Products in Local Markets ln Rivers State,Nigeria.

Risk of food borne infection contamination of food, in Africa is increased due to environmental, agronomic and socio-economic factors. This study assesses the microbial quality of melon and bush mango displayed in the local markets, aiming to provide valuable insights into the safety and quality of these widely consumed food products. Standard procedure for microbiological and proximate analysis were employed. The heterotrophic bacteria count ranged from 8.5 x 10⁵ cfu/g to 1.35 x10⁶ cfu/g. The total staphylococcus count ranged from 1.2 x 10³ cfu/g to 4.35 x 10³ cfu/g. The coliform count ranged from 2.8 x 10⁴ to 7 x 10⁵, Total Samonella count ranged from 4 x 10² cfu/g to 1.5 x 10³ cfu/g and Fungi count 6.5 x 10² cfu/g to 2.5 x 10³ cfu/g. Morphological and Biochemical characterization of heterotrophic bacteria isolated from both melon and bush mango identified nine genera, including Staphylococcus (14.6%) Bacillus (20%), Corynebacterium (5.6%) Enterobacter (4.4%), Micrococcus ( 3.3%) Pseudomonas (12.2%), Escherichia coli (13.3%), Salmonella (11.1%) and Proteus (11.1%). Fungal species like Penicillium spp., Fusarium spp., Aspergillus flavus, Candida spp., Mucor spp., Saccharomyces sp., and Aspergillus niger were isolated from both seed and milled seeds samples of melon and bush mango. The frequency of occurrence revealed variations among bacterial isolates in melon and bush mango seeds. The most frequent fungal species included Candida spp. (9.4%) Aspergillus niger (20%) Mucor (9.4%), Fusarium (11.4%), Aspergillus flavus (9.4%), Penicillium (13.2%), Rhizopus spp (3.7%) and Saccharomyces (15%). Proximate and heavy metal analysis indicated variations in the composition of Carbohydrate (7.11%), Lipid (15.03%), Ash (1.89%), Moisture (7.31%), Protein (23.86%), Crude Fiber (12.66%), Lead (0.213 mg/kg), Copper (2.11mg/kg), calcium (0.49mg/kg) and Cadmium (0.0169mg/kg) in melon and bush mango, Carbohydrate (15.03%), Lipid (58.24%), Ash (1.74%), Moisture (10.48%), Protein (11.95%), Crude Fiber (2.64%), Lead (1.7903mg/kg), Copper (2.11mg/kg), calcium (0.148mg/kg) and Cadmium (0.000385mg/kg). The presence of heterotrophic bacteria in such ranges suggests potential differences in hygiene practices during cultivation, processing, and storage. Therefore; Implement stringent quality control measures during the production, processing, and storage of both melon and bush mango seed.

Return of the Phrag-i: evaluating sexual reproduction mechanisms amenable to dieback recovery and potential invasiveness across Phragmites australis haplotypes

Phragmites australis is one of the most invasive wetland plants on the planet with both native and invasive haplotypes occurring in the United States. Three Phragmites haplotypes (Delta-, EU- and Gulf-types) co-occur in marshes of the Mississippi River Delta (MRD), where a recent dieback of Phragmites has prompted investigations about the potential for recolonization by seed. In other areas of the US, the invasive EU-type has been shown to spread by seed, yet little is known about reproduction modes of the Delta- and Gulf-types. We conducted a survey at 35 sites along the Mississippi River Delta region in southeast Louisiana to examine the potential for sexual reproduction across haplotypes as well as the potential for hybridization. Seed and pollen samples were collected from Phragmites populations to examine flowering phenology and determine pollen viability of the three lineages. We also conducted a seedbank assay in stands of three haplotypes to test the potential for recruitment by seed. Despite the observed potential for sexual reproduction in Delta- and EU- types, no Phragmites seedlings germinated from the seedbank. EU was the only haplotype to exhibit germination from seeds collected from seed heads. Both spatial separation and temporal isolation in flowering times indicate that hybridization between Phragmites haplotypes in the lower MRD is unlikely. High pollen production, increased pollen production following dieback, and viable seeds in the EU-type suggest that this invasive haplotype has a greater potential to invade new areas and adapt to stressors through sexual reproduction compared to than Delta-or Gulf haplotypes.

Headspace analysis of volatiles from commercial seeds of Trigonella foenum-graecum L. obtained from four Middle Eastern countries

ABSTRACT Trigonella foenum-graecum L. commonly known as fenugreek, is an important economic and medicinal plant cultivated in various regions of the world. Fenugreek produces several important active chemical constituents that determine its pharmacological properties. The aim of this research was to analyze the volatile constituents spontaneously emitted by eight samples of fenugreek seeds purchased from different Middle Eastern drugstores, including Saudi Arabia, Yemen, Egypt, and Iran. A total of 70 constituents belonging to different chemical classes, including monoterpene hydrocarbons, oxygenated monoterpenes, sesquiterpene hydrocarbons, phenylpropanoids, apocarotenes, nitrogen/sulfur derivatives, and non-terpene derivatives, were characterized in the volatile emissions of the eight samples, some components with significant olfactory properties were detected such as γ-nonalactone, cumin aldehyde, (E)-cinnamaldehyde, p-anisaldehyde, and eugenol. PCA showed two macro-clusters; the first comprised two sub-groups, while the second included only Yemeni samples. The findings of this study demonstrate that the significant variation for volatile profiles in fenugreek samples which are prone to variations in the geographical origin of these samples.

Morphometric Analysis on Sacha Inchi Plants (Plukenetia volubilis L.) using Organic Fertilizer Treatment of Chicken Manure

Sacha Inchi is a plant that has economic potential and is worthy of development. This research was conducted to understand the relationship between variables in the treatment of chicken manure liquid organic fertilizer. The research method used in this research was an experimental method on 200 samples of Sacha Inchi plant seeds. The analytical design used in this research is linear regression analysis. Based on the results of linear regression on chicken fertilizer treatment, it is known that the weight variable has an effect on the cocoa bean surface area variable by 51% (moderate), bean weight has an effect on thickness by 53% (moderate), the effect of thickness on bean surface area is 6.5% ( weak) and the volume influenced by seed weight is 81% (strong). Thus, increasing the weight of the seeds will increase the volume of the seeds.

Exploring the circular prospects of cashew nutshells and avocado seeds as potential adsorbents for onboard natural gas storage

Natural gas (NG), known for its eco-friendly energy attributes, faces challenges such as low volumetric density, leakage susceptibility, and explosion risks during storage and transport. To narrow this gap, this study reports on the potential of bio-based adsorbents from cashew nut shells (CNS) and avocado seeds (AVS) as an alternative approach for the onboard storage of NG. CNS and AVS were characterized to determine moisture content, volatile matter content, ash content, and fixed carbon content. Additionally, the study determined the optimal loading of activating agents to produce activated carbons (ACs). ACs were produced through a dual process involving simultaneous chemical activation and carbonization. In the chemical activation phase, a sample of either cashew nut shells (CNS) or avocado seeds (AVS) was impregnated with phosphoric acid at various ratios. Subsequently, the properties of the ACs, including surface area, pore volume, and X-ray diffraction (XRD), were evaluated. The NG adsorption capacity of the ACs was assessed, and Freundlich and Langmuir isotherm adsorption equations were derived to elucidate the NG adsorption behavior on the ACs. The results revealed that the proximate analysis of CNS and AVS showed low moisture content (6.48% and 8.91%), high volatile matter (80.21% and 73.09%), low ash content (1.48% and 4.71%), and high fixed carbon (13.65% and 15.59%), indicating suitability for AC formulations. During synthesis, adding phosphoric acid to AVS or CNS in a 1:1 ratio resulted in significantly larger surface areas and pore volumes in the produced ACs. X-ray diffraction analysis of the ACs produced from CNS or AVS with phosphoric acid added at a 1:1 ratio indicated a broad diffraction background and a predominantly amorphous structure. The performance of the ACs in the adsorption of NG demonstrated that ACs produced from 1:1 mixtures of CNS with phosphoric acid and AVS with phosphoric acid exhibited higher adsorption capacities, 0.038 g/g and 0.031 g/g, respectively, at a low pressure of 5 bar, showcasing a novelty of this study. Furthermore, the Freundlich equation test yielded a constant R² exceeding 0.9, with positive adsorption intensity and adsorption capacity values, indicating a favorable fit for the model. In contrast, the Langmuir equation test resulted in a constant R² exceeding 0.9, but the adsorption capacity exhibited negative values, leading to inconclusive explanations. These findings affirm that bio-wastes can be an effective source of adsorbents for onboard natural gas storage , suggesting a means to reduce the take-dispose model and promote a circular economy in the energy sector.

Effect of Desiccants and Packaging Materials on Seed Storability in Soybean [Glycine max (L.) Merrill

Background: Seeds are characterized by rapid deterioration during storage under hot and humid coastal conditions. Physiological changes that occur in seeds during storage are manifested as reduction in seedling vigour indices and percentage germination. This study aimed in prolonging the longevity of soybean seed through packaging materials with or without desiccants. Methods: Each 10 kilograms of soybean seeds treated with Vitavax 200 (2 g/kg of seed) were stored with or without desiccants viz., Calcium Chloride @ 10 g/kg of seeds, Silica gel @ 10 g/kg of seeds and ‘Zeolites’ drying beads @ 35 g/kg of seeds, packed in two different containers viz., Cloth bag and Super grain bag and stored under ambient conditions of coastal environment of Karaikal district, Puducherry (UT). Seed samples were drawn at three months intervals and tested for seed vigour and viability up to nine months. Result: The results revealed that that vitavax treated soybean seeds stored in Super grain bags with desiccants viz., CaCl2 or drying beads exhibited the highest seed germination, seedling length, seedling dry weight, vigour index-I and II than cloth bags after nine months of storage. The loss in seed vigour during storage was only 12 per cent in Super grain bags as compared to 83 per cent in cloth bags indicated that Super grain bag has the potential to effectively contain the influence of environment on seeds stored in it. Hence, it is suggested that the vigour and viability of soybean seeds could be very well prolonged by storing fungicide treated seeds in Super grain bag instead of cloth bags under coastal environment.

Seed Science and Technology. Volume 52 Issue 1 (2024)

As the International Seed Testing Association (ISTA) celebrates its 100-year anniversary in 2024, we can also commend its specific role in supporting research on seeds. Indeed, one of ISTA's objectives is "To actively promote research and dissemination of knowledge in seed science and technology, for the sampling, testing, storing, processing and distribution of seeds". One of the main ways in which scientific knowledge of seeds is disseminated by ISTA is through its scientific journal,Seed Science and Technology, which itself celebrated its 50thvolume in 2022 with a special issue of review articles and editorial (Jones, 2022). The review articles covered some of the history of research on particular aspects of seed testing, and gave up-to-date summaries of our current understanding of topics such as vigour, seed storage, tetrazolium testing, etc. Collectively, they cite many original research papers published inSeed Science and Technology. Since 2003,Seed Science and Technologyhas been available electronically at https://www.ingentaconnect.com/content/ista/sst, and articles published since 2018 are open-access, meaning that they are available for anyone to read for free. The 1973 to 2002 back issues of SST are available from ISTA at publication. sales@ista.ch. The articles in this latest issue of SST will similarly each advance our understanding of seed processes and responses, continuing to contribute to the body of knowledge on seeds. The authors of the articles in this issue cover different regions of the world, emphasising the global nature of seed science and the ISTA community.

Detection the quality of pumpkin seeds based on terahertz coupled with convolutional neural network

AbstractPumpkin seeds are nutritious and have some medicinal value. However, the mold and sprouting are produced during the storage of pumpkin seeds. Food safety and quality problems may be caused if they are not removed in time for processing. The traditional testing methods are cumbersome to operate, complex, and destructive in sample preparation. Therefore, terahertz time‐domain spectroscopy (THz‐TDS) technology was proposed to achieve the detection of the internal quality of pumpkin seeds. Firstly, samples of pumpkin seeds of different qualities were crafted, and they were moldy for 3 days, moldy for 6 days, sprouted and moldy, sprouted and normal pumpkin seeds, respectively. Then, the pumpkin seeds of different qualities were dried, ground, and pressed, and their spectral data were collected. The terahertz spectra of the five types of samples were significantly different. The support vector machine (SVM), random forest (RF), and convolutional neural network (CNN) qualitative discriminant models were established with the raw absorbance spectral data, the preprocessed absorbance spectral data, and the preprocessed and band‐screened absorbance spectral data, respectively, where the CNN model based on the raw spectral data has the highest classification accuracy of 96%. The CNN models do not require advance spectral data processing, simplifying the spectral analysis process. And it achieves best classification results in the accuracy of detection compared to traditional chemometric models. The CNN combined with THz‐TDS method has great potential for application in the detection of agricultural products. It provides a new detection method for the field of quality detection of agricultural products.