Field trial was conducted in 2006, 2007 and 2008 wet seasons, at the Institute for Agricultural Research Farm, Samaru in the Northern Guinea savanna of Nigeria to determine the effects of nitrogen and sulfur fertilizers on nitrogen uptake of quality protein maize (QPM) varieties. Treatments consisted of four (4) rates each of nitrogen (0, 60,120 and 180 kg N/ha) and sulfur fertilizer (0, 5, 10 and 15 kg S/ha) and two QPM varieties (Obatampa and EV – 99), laid out in a split plot design with variety and nitrogen in the main plots and sulfur in the sub plots and replicated three times. The results show that varietal differences influenced soil and flag leaf nitrogen in 2006 and 2008 respectively. Nitrogen application did not affect nitrogen content in the soil at all the three years of the trial. However, Ear, Flag and Grain nitrogen content was affected by nitrogen application. Sulfur application influenced soil, ear leaf, flag leaf and grain nitrogen content in different years of the study.