The major purpose of this research was to introgress Saltol QTL into the genetic background of BRRI dhan49 through marker-assisted backcrossing. FL478 was used as a donor parent of Saltol QTL. Marker assisted backcrossing strategies were applied to develop BRRI dhan49-Saltol lines. A primer polymorphism survey was carried out between the two parental genotypes viz. BRRI dhan49 and FL478. A total of 363 SSR and InDel primers were surveyed and a total of 96 markers (27%) were found polymorphic. A cross was made between BRRI dhan49 and FL478 to produce F1 seeds and F1 was confirmed by RM493. Foreground selection was carried out using RM493 in all generations. Fifty six SSR markers were used for background selection. The Graphical Genotype (GGT) software was used to estimate the percentage recovery rate of the recurrent parent genome and to find out the genome ratio of the parents in the selected progenies of the backcross populations. From BC1F1 generation five best plants were selected based on background recovery of recurrent parent and backcrossed with recurrent parent. From BC2F1 generation, six plants were selected based on the highest background recovery of 80.7% to 89.5%. BC2F2 seeds were produced by selfing of selected BC2F1 individuals. The Saltol introgressed lines performed better in salt stress condition in hydroponic screening. The developed BRRI dhan49-Saltol lines would be useful for developing salt tolerant rice varieties or for using in other breeding programs.