Salt Lake In China Research Articles

Lentibacillus lacisalsi sp. nov., a moderately halophilic bacterium isolated from a saline lake in China

An aerobic, spore-forming, moderately halophilic bacterium, strain BH260(T), was isolated from a salt lake in China. Cells of this strain were found to be motile rods with flagella. The organism grew optimally at 30-32 degrees C and pH 8.0 in the presence of 12-15% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate formed a clade with the type strains of the genus Lentibacillus. The levels of 16S rRNA gene sequence similarity for strain BH260(T) with respect to Lentibacillus salsus KCTC 3911(T), Lentibacillus juripiscarius JCM 12147(T) and Lentibacillus salicampi KCCM 41560(T) were 96.0, 95.5 and 95.3%, respectively. Chemotaxonomic data (a DNA G+C content of 44 mol%; MK-7 as the major isoprenoid quinone; A1gamma-meso-diaminopimelic acid as the cell-wall type; phosphatidylglycerol and diphosphatidylglycerol as the major polar lipids; anteiso-C(15:0), anteiso-C(17:0) and iso-C(16:0) as the major fatty acids) also supported assignment of the isolate to the genus Lentibacillus. On the basis of physiological and molecular properties, the isolate represents a novel species within the genus Lentibacillus, for which the name Lentibacillus lacisalsi sp. nov. is proposed. The type strain is BH260(T) (=KCTC 3915(T)=DSM 16462(T)).

Halobiforma lacisalsi sp. nov., isolated from a salt lake in China

A Gram-negative, motile, neutrophilic and extremely halophilic strain, AJ5(T), was isolated from a salt lake in Xinjiang, China, and subjected to polyphasic taxonomic study. The major polar lipids of the isolate were C(20)C(20) and C(20)C(25) derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and glycolipid. The DNA G+C content was 64.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain AJ5(T) clustered with members of the genus Halobiforma, exhibiting high sequence similarity to the 16S rRNA gene sequences of Halobiforma nitratireducens (96.3%) and Halobiforma haloterrestris (99.0%). Comparative analysis of phenotypic characteristics and DNA-DNA hybridization between strain AJ5(T) and Halobiforma species supported the conclusion that AJ5(T) represents a novel species within this genus, for which the name Halobiforma lacisalsi sp. nov. is proposed. The type strain is AJ5(T) (=CGMCC 1.3738(T)=JCM 12983(T)).

Natrinema versiforme sp. nov., an extremely halophilic archaeon from Aibi salt lake, Xinjiang, China.

A novel extremely halophilic archaeon, strain XF10T, was isolated from a salt lake in China. This organism was neutrophilic, non-motile and pleomorphic, and was rod, coccus or irregularly shaped. It required at least 1.5 M NaCl for growth and grew in a wide range of MgCl2 concentrations (0.005-0.5 M). Lipid extract of whole cells contained two glycolipids with the same chromatographic properties as two unidentified glycolipids found in the two described Natrinema species, Natrinema pellirubrum and Natrinema pallidum. Phylogenetic analysis based on 16S rDNA sequence comparison revealed that strain XF10T clustered with the two described Natrinema species and several other strains (strains T5.7, GSL-11 and Haloterrigena turkmenica JCM 9743) with more than 98.1% sequence similarities, suggesting that strain XF1OT belongs to the genus Natrinema. Comparative analysis of phenotypic properties and DNA-DNA hybridization between strain XF10T and the Natrinema species supported the conclusion that strain XF10T is a novel species within the genus Natrinema. The name Natrinema versiforme sp. nov. is proposed for this strain. The type strain is XF10T (=JCM 10478T=AS 1.2365T=ANMR 0149T).

A preliminary investigation on the determination of lanthanoids, thorium and uranium in brine and deposit of a salt lake in China

A brine sample and two deposit samples of a salt lake in China were analyzed for their contents of lanthanoids (Ln's), thorium and uranium by neutron activation analysis. Five Ln's were determined at sub ppb levels. Th and U contents were at about the same levels as those of Ln's. The lanthanoid abundance patterns (Ln pattern) of the three samples are similar to each other, each having a negative slope in the light Ln region. There seems no substantial difference in distribution between the solution (brine) and solid (deposit) phases among Ln's.

Visiting Report to Salt Lake in China

Visiting Report to Salt Lake in China