Salt Extraction Method Research Articles

Physico-chemical and biological properties of pumpkin pectin

This study presents the findings of pectin's isolation and purification process derived from the Michurinskaya pumpkin variety. The researchers employed infrared spectroscopy and acid-base titration techniques to determine the presence of free carboxyl groups and the degree of esterification. Additionally, the physicochemical properties of the resulting polysaccharide, including kinematic viscosity and molecular weight, were investigated using viscometry. Furthermore, the fungicidal activity of the resulting polysaccharide was assessed. The acidic polysaccharide, pectin, was extracted from the Michurinskaya pumpkin cultivar using the salt extraction method, yielding 29 %. The overall concentration of OM e- and OAc groups (36.3%) and free carboxyl groups (6.48%) was measured using acid-base titration techniques. Infrared (IR) spectroscopy data validated the obtained results. The spectra revealed signals inside the 1319-1408 cm-1 region, corresponding to OMe groups. Free carboxyl groups were also present in the 1730-1760 cm-1 range. The molecular weight of pectin measured using the viscometry method was 2.5 kDa. The findings of the antifungal activity investigation indicated that the isolated pectin derived from pumpkins demonstrates inhibitory properties against the growth of Penicillium sp.

Compositional, structural, and functional characterization of fucoidan extracted from Sargassum polycystum collected from Saint Martin's Island, Bangladesh

Fucoidan is a sulphated polysaccharide with diversified biological activities commonly found in brown seaweeds. In our present study, fucoidan was extracted from Sargassum polycystum collected from Saint Martin's Island in three different extraction methods. The composition of the fucoidans was determined by measuring the total carbohydrate, sulfate, uronic acid, fucose, and protein contents. Fourier-transform infrared spectroscopy (FT-IR) and Proton nuclear magnetic resonance (1H NMR) were studied to determine the structural groups of extracted fucoidans. Functional activities were analyzed by antioxidant activity and anti-diabetic activity assay. Fucoidan yield was obtained highest in the acid extraction method (0.061 ± 0.007 g/g), followed by the water extraction method (0.058 ± 0.005 g/g) and salt extraction method (0.041 ± 0.005 g/g). Water extracted fucoidan showed the highest carbohydrate content (62.97 %), uronic acid content (6.46 %), and protein content (2.62 %). Sulphate content (28.56 %) was highest in salt extracted fucoidan, whereas fucose content (24.0 %) was highest in water extracted fucoidan. FT-IR and 1H NMR analysis confirm several characteristic picks for the extracted fucoidans. Extracted fucoidans presented comparable antioxidant activity, but salt-extracted fucoidan presented the highest DPPH radical scavenging assay (86.85 ± 0.74 %, IC50 = 0.03 mg/mL), ABTS radical scavenging assay (65.13 ± 0.92 %, IC50 = 1.94 mg/mL), Phosphomolybdenum assay (4.356 ± 0.11) and Nitric Oxide (NO) radical scavenging assay (57.08 ± 0.52 %, IC50 = 3.73 mg/mL) in dose-dependency manner. Further anti-diabetic activity assay resulted in the highest α-amylase inhibition activity (62.82 ± 1.36 %, IC50 = 2.30 mg/mL) and α-glucosidase inhibition activity (55.73 ± 0.84 %, IC50 = 3.71 mg/mL) by salt-extracted fucoidan. These functional potentials of seaweed may add significant value to the development of functional food and pharmaceutical materials.

Validation of vacuum extraction protocol for larval sampling of spotted-wing drosophila in blueberries

For spotted-wing drosophila, Drosophila suzukii Matsumura, the standard larval sampling protocol from the fruits involves immersing the fruits in hot water, salt, or sugar solution followed by enumerating the larvae that exit from the fruit. In this study, using the fruit samples collected from commercial blueberry farms, we validated the efficacy of a novel D. suzukii larval sampling technique, the vacuum extraction protocol. The larval extraction efficacy of the vacuum extraction method was compared with the salt extraction method using paired fruit samples collected from three different fields for four consecutive weeks. Overall, larval counts detected through the vacuum extraction were significantly higher than those detected through salt extraction in two out of three blueberry fields. Additionally, among the five blueberry fruit sample volumes tested (118, 177, 237, 355, and 473 mL), we identified 355 mL (12 fl. oz.) as the optimum fruit sample volume for vacuum extraction that maximized sampling precision for unit sampling cost. Since D. suzukii larvae exited from fruits after vacuum extraction remain alive and active, a delay in separating the larvae from the sample with coffee filter extraction may result in larval movement back into the fruit flesh. However, compared with immediate processing, delaying coffee filter extraction up to 60 minutes after vacuum incubating the fruit samples at -98 kPa for 60 minutes did not significantly reduce the larval count. Overall, the results of validation trials indicate that vacuum extraction is a promising sampling method for growers to detect D. suzukii larval infestation in blueberries.

Integrated DNA barcoding methods to identify species in the processed fish products from Chinese market

DNA-based methods are reliable for a precise identification of species in processed products. In this study, we assessed five typical DNA extraction methods from multiple aspects. Full-length and mini-length DNA barcoding were performed to detect the species substitution and mislabeling of 305 processed fish products from the Chinese market covering six processed fish products. The salt extraction method that exhibited the best overall performance was applied. All samples were successfully extracted; however, only 19.3 % of samples could be amplified using the full-DNA barcode primer set, and 90.2 % of samples could be amplified using the newly designed mini-DNA barcode primer sets (401 and 320 bp). Overall, the molecular identification results revealed that 36.4 % (111/305) of the samples were inconsistent with the labels, with commercial fraud observed in all six types of processed fish products. The survey findings provide technical references for effective fish authentication monitoring, offering insights into the seafood safety in markets.

A comparative analysis of different DNA extraction methods for fish tissues

Nucleic acids extraction is the initial stage of molecular genetic research. The effectiveness of using a certain method of DNA extraction is determined by the amount of extracted DNA, the degree of probe degradation, the time consuming and laboriousness of the procedure. When researching rare, endangered species and populations, special attention is paid to extracting DNA from sources that do not cause destruction or death of individual. The current study was aimed at comparison the effectiveness of the most common methods of genomic DNA isolation from fish tissues for further use in the polymerase chain reaction. Essentially three DNA extraction methods were employed and compared for the quality of isolated DNA ‒ the phenol-chloroform extraction method, the salt extraction method, and the sodium perchlorate extraction method. Samples of fins, liver and caver taken from sterlet individuals fixed in ethanol served as material for research. The study of the effect of DNA probe quality on PCR course was carried out using two types of PCR ‒ site-specific, amplifying the fragment of cytochrome-b gene, and multilocus ‒ ISSR-PCR with a trinucleotide primer (AGC)6T. A comparison of DNA extraction methods confirmed that all of them enable obtaining high-quality DNA from both fresh and archival fish tissue samples for site-specific amplification. The yield of nucleic acids using different fish tissues (fins, liver and caver) is not the same. Therefore, when it is necessary to obtain the maximum amount of DNA, it is advisable to use the salt extraction method. In case of ISSR-PCR, different degrees of DNA purification affected the course of amplification. Additional purification by the method of sorption on Silica allows removing possible PCR inhibitors and obtaining clear spectra of electrophoretic separation of amplification products, regardless of primary DNA quality. Key words: DNA extraction methods, sterlet tissues, site-specific PCR, multilocus PCR.

The Current Situation of Pea Protein and Its Application in the Food Industry.

Pea (Pisum sativum) is an important source of nutritional components and is rich in protein, starch, and fiber. Pea protein is considered a high-quality protein and a functional ingredient in the global industry due to its low allergenicity, high protein content, availability, affordability, and deriving from a sustainable crop. Moreover, pea protein has excellent functional properties such as solubility, water, and oil holding capacity, emulsion ability, gelation, and viscosity. Therefore, these functional properties make pea protein a promising ingredient in the food industry. Furthermore, several extraction techniques are used to obtain pea protein isolate and concentrate, including dry fractionation, wet fractionation, salt extraction, and mild fractionation methods. Dry fractionation is chemical-free, has no loss of native functionality, no water use, and is cost-effective, but the protein purity is comparatively low compared to wet extraction. Pea protein can be used as a food emulsifier, encapsulating material, a biodegradable natural polymer, and also in cereals, bakery, dairy, and meat products. Therefore, in this review, we detail the key properties related to extraction techniques, chemistry, and structure, functional properties, and modification techniques, along with their suitable application and health attributes.

DNA Extracted from Byproducts of Common Carp Testis and Application in Removing Ethidium Bromide from Pollutants

ABSTRACT In the present paper, an optimized method to extract common carp sperm DNA (csDNA) from common carp testis, the mechanism of ethidium bromide (EtBr) interaction with csDNA, as well as the removal of EtBr pollutant by csDNA was studied. The results showed that high salt extraction method was suitable for extracting csDNA. Under the optimum conditions, the yield of csDNA extraction was as high as 11.7%. In addition, the results of UV–vis spectra, fluorescence spectra, and observation with fluorescence microscope indicated that csDNA could be intercalated by EtBr to form a stable complex, which confirmed that csDNA could be applied in removing EtBr pollutant. Based on the csDNA intercalation, EtBr pollutant was removed by csDNA effectively, which was 94.13%. This work not only provides a smart method to extract high purity and quality csDNA from byproducts of common carp testis but also provides an easily available csDNA to remove EtBr from pollutants.

Extraction, nutrition, functionality and commercial applications of canola proteins as an underutilized plant protein source for human nutrition.

Concerns about sustainability and nutrition security have encouraged the food sector to replace animal proteins in food formulations with underutilized plant protein sources and their co-products. In this scenario, canola protein-rich materials produced after oil extraction, including canola cold-pressed cakes and meals, offer an excellent opportunity, considering their nutritional advantages such as a well-balanced amino acid composition and their potential bioactivity. However, radical differences among major proteins (i.e., cruciferin and napin) in terms of the physicochemical properties, and the presence of a wide array of antinutritional factors in canola, impede the production of a highly pure protein extract with a reasonable extraction yield. In this manuscript, principles regarding the extraction methods applicable for the production of canola protein concentrates and isolates are explored in detail. Alkaline and salt extraction methods are presented as the primary isolation methods, which result in cruciferin-rich and napin-rich isolates with different nutritional and functional properties. Since a harsh alkaline condition would result in an inferior functionality in protein isolates, strategies are recommended to reduce the required solvent alkalinity, including using a combination of salt and alkaline and employing membrane technologies, application of proteases and carbohydrases to facilitate the protein solubilization from biomass, and novel green physical methods, such as ultrasound and microwave treatments. In terms of the commercialization progress, several canola protein products have received a GRAS notification so far, which facilitates their incorporation in food formulations, such as bakery, beverages, salad dressings, meat products and meat analogues, and dairies.

Extraction behaviors of aqueous PEG impregnated resin system in terms of impregnation stability and recovery via protein impregnated resin interactions on bovine serum albumin.

BackgroundCurrent advances in biotechnology have been looked at as alternative approaches towards the limited product recovery due to time- and cost-consuming drawbacks on the conventional purification methods. This study aimed to purify bovine serum albumin (BSA) as an exemplary target product using an aqueous impregnated resin system (AIRS). This method implies the concept of hydrophobicity of polymer that impregnated into the resins and driven by electrostatic attractions and hydrophilicity of aqueous salt solution to extract the target product.MethodsThe extraction behaviors of impregnation in terms of stability and adsorption kinetics via protein-aqueous polymer impregnated resin were studied. Impregnation stability was determined by the leaching factor of polyethylene glycol (PEG). The major factors such as PEG molecular weights and concentration, pH of aqueous salt solution, extraction methods (sonication and agitation) and types of adsorbent material and concentration of aqueous salt phase influencing on partitioning of biomolecule were also investigated.ResultsFor impregnation stability, the leaching factor for Amberlite XAD4 did not exceed 1%. The scanning electron microscopy (SEM) image analysis of Amberlite XAD4 attributes the structural changes with impregnation of resins. For adsorption kinetics, Freundlich adsorption isotherm with the highest R2 value (0.95) gives an indication of favorable adsorption process. Performance of AIRS impregnated with 40% (w/w) of PEG 2000 was found better than aqueous-two phase system (ATPS) by yielding the highest recovery of BSA (53.72%). The outcomes of this study propound the scope for the application of AIRS in purification of biomolecules.

Effects of ultrasound-assisted salt (NaCl) extraction method on the structural and functional properties of Oleosin

22Abbreviations. AFM: atomic force microscopy; SH: Sulfhydryl; UV–vis: Ultraviolet–visible; CD: circular dichroism; WAC: water absorption capacity; FAC: fat absorption capacity; EAI: emulsifying activity index.We propose a new ultrasound-assisted salt (NaCl) Oleosin extraction method, where the addition of NaCl induces the dissociation of Oleosin subunits and promotes the unfolding of the protein spatial structure. The yield of Oleosin post extraction and purification and solubility of Oleosin obtained using the proposed method were higher than those of Oleosin extracted using traditional methods, by 17.6% and 122.9%, respectively; reduction in particle size (to 52 nm) was also noted. Hydrogen bond dissociation, increase in surface hydrophobicity, and disulfide bond formation occurred simultaneously. However, the overall structure of Oleosin was not negatively affected. The physical properties of Oleosin, such as water and oil absorption, emulsification, and antioxidant activity, were improved, and the rate of Oleosin digestion decreased during the in vitro simulated digestion process. The proposed method provides a theoretical basis for producing proteins. This method can be utilized for effective extraction of Oleosin to achieve sustained release of the produced proteins.

Kidney bean protein products as potential antioxidative and antihypertensive alternatives for non-pharmacological inhibition of angiotensin-converting enzymes

Kidney bean protein (KBP) albumin (KBPA) and globulin (KBPG) were produced by salt extraction method while the isolate (KBPI) was prepared by alkaline extraction followed by isoelectric precipitation. Furthermore, enzymatic hydrolysates of KBPI were prepared by treatment with alcalase (KBPH-A) and pepsin+pancreatin (KBPH-PP) respectively, while the antioxidant and angiotensin-converting enzyme (ACE) inhibiting activities of KBP products were evaluated after determining their amino acid (AA) composition profile. The result of the AA profile showed that while the hydrophobic amino acid (HAA) of KBPG (41%) was higher than KBPA (39%) and KBPI (36%) respectively, they however had similar (11%) aromatic amino acids (AAA). In addition, enzymatic hydrolysis resulted in increased contents of HAA and AAA of KBPH-A and KBPH-PP (42–45%; 14%), respectively. The KBPG had the highest OH− and ABTS− scavenging activities (40 and 45%) among the non-hydrolyzed fractions, which increased (70 and 75%) after hydrolysis by alcalase and PP. The KBPG had higher bioactivities (antioxidant and ACE inhibition) than the KBPA, which might be due to more hydrophobic character resulting from the higher disulfide bond present in globulin compared to the glycoprotein albumin. The hydrolyzed proteins had significantly higher (p<0.05) DPPH scavenging, metal chelation and ferric reducing activities than the non-hydrolyzed KBP. The ACE-inhibitory ability of KBP products was positively related to their AA profile, suggestive of the role of hydrophobicity, strong disulfide bonds and hydrolysis during inhibition. The increased ACE inhibitions by KBPH-PP (93%) compared to KBPH-A (85%) confirmed the effectiveness of dual endo- and exo-peptidase activities of PP over single endo-peptidase alcalase. This study presents valuable information on the production of potential compounds (protein hydrolysates) from an edible plant protein source for use in the management of cardiovascular diseases.

First molecular record of the alien species Pacific oyster (Crassostrea gigas, Thunberg 1793) in the Marmara Sea, Turkey

The Pacific oyster (Crassostrea gigas) has a very important economic potential for aquaculture, but on the other hand, is among the highly invasive species in the world and within the Mediterranean ecosystem. In the 1960s, C. gigas was brought to Europe for aquaculture in the Mediterranean and Black Sea regions from Japan and Canada. The Turkish waters are the part of the Mediterranean Sea, which is the world’s most invaded sea. The invasion of alien species results from marine transportation and aquaculture activities of non-native species. A heavy maritime traffic is also present in the Marmara Sea, which connects the Black Sea and Mediterranean Sea. The identification of the invasive species and their distributions is very prominent in terms of protecting natural habitat and monitoring the effects of invasive species. In this study, 30 individuals, morphologically identified as C. gigas, were collected from Bandırma bay. The genomic DNAs were extracted from each sample’s muscle tissue using universal salt extraction method. Partial sequences of COI and 16S Mitochondrial DNA loci of the sample DNAs were obtained for species identification. The sequences were searched against the database and results were retrieved from BLAST. All the sequences obtained in this study showed significant similarity with the C. gigas sequences present in the database (E=0). The sample sequences resulted in 9 different haplotypes for the COI locus (hd: 0.5296 and variance: 0.01256±0.112) and 5 different haplotypes for the 16S rDNA locus (hd: 0.2529, Variance: 0.01076±0.104). The results of this study provided the first molecular evidence for the presence of non-native Pacific oyster individuals in the Marmara Sea.

Challenging TaqMan probe-based real-time PCR and loop-mediated isothermal amplification (LAMP): the two sensitive molecular techniques for the detection of toxoplasmosis, a potentially dangerous opportunistic infection in immunocompromised patients.

Due to defects and drawbacks of most conventional diagnostic methods including serology for the diagnosis of toxoplasmosis as a dangerous opportunistic infection in immunocompromised individuals, the accurate, rapid, and sensitive detection of infection in such patients is essential. In this study, the TaqMan probe-based real-time PCR and, a relatively new nucleic acid amplification method, the loop-mediated isothermal amplification (LAMP) technique was compared based on the repetitive elements (RE) sequence to detect Toxoplasma gondii (T. gondii) DNA in blood samples of immunocompromised individuals. During this study, 119 blood samples from immunocompromised cancer patients with renal failure, undergoing dialysis were studied. After DNA extraction from blood samples using the salt extraction method, the molecular techniques of TaqMan probe-based real-time PCR and LAMP were used to investigate the contamination of the samples with T. gondii, based on the 529bp (RE) sequence of T. gondii. The analytical sensitivity of LAMP and real-time PCR was evaluated by duplicating the five-step serial dilutions of T. gondii tachyzoites from 0.25 to 5×105 spiked tachyzoites per milliliter of the Toxoplasma seronegative blood sample. The extracted DNA from other parasites and human chromosomal DNA were used to determine the specificity of the molecular methods. The obtained results were analyzed using Kappa statistical test and SPSS22 software. Out of 119 studied samples, 7 (5.8%) and 5 (4.2%) samples were positive for Toxoplasma by TaqMan probe-based real-time PCR and LAMP, respectively. The limits of detection of TaqMan probe-based real-time PCR and RE-LAMP in negative serum samples were one and five tachyzoites (CT 38), respectively. Both real-time PCR and LAMP methods were 100% specific for Toxoplasma detection. Positive results were obtained only with T. gondii DNA, while other DNA samples were negative. The TaqMan probe-based real-time PCR based on the RE sequence showed higher sensitivity to T. gondii DNA detection in blood samples of cancer patients and serial dilutions of parasitic tachyzoites. The results show that TaqMan probe-based real-time PRC is a sensitive and specific method for the detection of toxoplasmosis in immunocompromised individuals, as well as the LAMP assay, which can be used as a suitable alternative diagnostic method for the detection of toxoplasmosis in such patients, without need the for any expensive equipment.

POPULATION GENETICS OF LONGTAIL TUNA (Thunnus tonggol) (BLEEKER, 1851) FROM EAST MALAYSIA BASED ON MITOCHONDRIAL DNA D-LOOP MARKER

Present study investigates the genetic diversity and genetic distribution of the longtail tuna Thunnus tonggol collected from east Malaysia (Borneo states of Sabah and Sarawak) based on mitochondrial DNA D-loop sequence analysis. 58 fish samples were obtained, specifically from Kota Kinabalu, KK (n = 22), Miri, MR (n=20) and Bintulu, BT (n = 17). DNA template was isolated using the salt extraction method. Final length of 404 base pair (bp) D-loop sequences revealed 52 haplotypes that comprise of 77 variable sites (38 of parsimony informative and 39 singleton). A total of 20 haplotypes were found in KK, 19 haplotypes in MR and 16 haplotypes in BT. Molecular diversity indices revealed high haplotype diversity and low nucleotide diversity in all populations; KK (h = 0.9913 ± 0.0165, π = 0.00239 ± 0.0127), MR (h = 0.9942 ± 0.0193, π = 0.0226 ± 0.0121) and BT (h = 0.9926 ± 0.0230, π = 0.0196 ± 0.0171). Population comparison pairwise FST show that KK and BT were significantly genetically differentiated. The result from this study will be beneficial for fisheries management and also to provide information on the population genetics of T. tonggol in East Malaysian waters.

Evaluating the production efficiency, purity and chemical compounds of the Vicia ervilia protein isolates produced by different methods of extraction

Introduction: Vicia ervilia, known as bitter vetch is an ancient grain legume crop from Poaceae family. Due to its low cost and production capability in Iran and having high protein content, the resulted flour and its protein products can be evaluated in terms of usability in the food industry. This study was aimed to evaluate the production efficiency, purity and chemical compounds of the V. ervilia protein isolates produced by different methods of alkali and acid extraction–sedimentation at isoelectric point, dialysis –salt extraction and miscella sedimentation Methods: In this study, V. ervilia was provided from the Agricultural Jihad Organization of Lorestan province and the protein isolates of V. ervilia were produced using different methods of protein extraction such as Acidic extraction-sedimentation at isoelectric point, Alkaline extraction-sedimentation at the isoelectric point, dialysis-salt extraction and extraction by miscella sedimentation. Results: The results showed that saline extraction methods (salt-dialysis and miscella) were significantly more effective than the isoelectric sedimentation methods (alkaline and acidic) on increasing the efficiency, purity and protein content of isolates and decreasing the impurities and carbohydrates. Conclusion: The results of this research show that the salt extraction methods (salt-dialysis and miscella) are significantly more effective in increasing the efficiency, purity and protein rate of isolates and in decreasing impurities and carbohydrates than the isoelectric sedimentation methods (alkaline and acidic).

Investigating the physicochemical, sensory and microbial properties of plant-based protein products (meat-free burgers) formulated using various Vicia ervilia (L.) Willd. protein isolates

The present study investigated the effect of using various protein isolates of Vicia ervilia (L.) Willd. (Bitter vetch; Family: Fabaceae) in formulating plant burgers on physicochemical, sensory and microbial properties of the resulting plant-based protein products. The results indicated that the plant-based protein products in the formulation of which protein isolates produced based on salt extraction methods were used, had higher rates of protein, fat and oil. On the contrary, they had lower rates of carbohydrate and moisture in contrast to the plant-based protein products formulated using protein isolates produced based on isoelectric precipitation methods. The reason for the high amount of fat and protein in these treatments can be attributed to the isolates’ production conditions, high amount of oil diffused from the product in contrast to low oil retention and absorption capacity and low level of the measured moisture in comparison with the high protein content. In the current century, due to the uneven human population growth as well as the limited growth of animal resources, the debate about protein deficiency is particularly important in developing societies. Efforts for finding abundant and cheap herbal sources of protein have led to various researches regarding the use of some such less-known legumes in developing countries. In this regard, different protein isolates of Vicia ervilia have been used in the formulation of vegetable burgers to produce a new, inexpensive and productive plant protein product in the food industry.

Functional properties of Grass pea protein concentrates prepared using various precipitation methods.

Pulses are an affordable source of proteins, starch, lipids, minerals and high value nutritional sources. This study was conducted to evaluate relationship between protein functional properties and their preparation methods. Therefore, the functional properties of Grass pea protein concentrates (GPPC) prepared using isoelectric precipitation (IE), salt extraction (SE) and ultrafiltration-diafiltration methods (UF/D) were determined. The GPP processed by those three precipitation methods contained all of the amino acids which aspartic acid and glutamic acid were dominate amino acids followed by arginine and leucine. However, methionine and tryptophan were limited amino acids. Water binding capacity was in following order: UF/D-GPPC > SE-GPPC > IE-GPPC. Meanwhile, highest value of oil binding capacity belonged to UF/D-GPPC. GPPC prepared using UF/D method had highest solubility. In term of interfacial tension, it was revealed that the interfacial tension of all isolates did not significantly reduced (P > 0/05). Net negative zeta potential with values was observed which IE-GPPC had highest surface charge followed by UF/D-GPPC and SE-GPPC, respectively. In terms of surface hydrophobicity, it was altered in the following order: IE-GPPC > SE-GPPC > UF/D-GPPC. It was observed that foaming capacity ranged between 85.06 and 89.78% and foaming stability ranged between 77.34 and 84.35%. Emulsifying capacity, emulsifying activity index and emulsifying stability index ranged between 105.06-109.78%, 31.09-36.29m2/g and 12.90-18.86min respectively. Evaluation of least gelling concentration showed that UF/D-GPPC were capable to form firm gel at low concentration (10% W/V). The functional properties of proteins are influenced by their extraction technique and can be achieve maximum functional characteristics by selecting appropriate extraction method. The results indicated the technological potential of GPP for health-promoting food formulations.

Optimization process of moringa oleifera seed extract using artificial neural network (ANN)

The use of chlorine which causes disinfection of by-products is a major concern especially in the developed countries. There is the need to look for a cheap alternative such as the use of plant material as substitute for chemical disinfectant. Moringa oleifera is an extensively documented plant material used for the treatment of drinking water. Its seed extracts contain active agents that having excellent coagulation properties and exerting in-vitro bactericidal activity. However, lack of available literature on the statistical optimization using artificial neural network (ANN) for inactivation kinetics of the seed extract using different disinfection models is the major aspect that need to be explored. This study was conducted to develop operation parameters using ANN for the seed extracts to be used as disinfectant for water treatment. The optimization process based on statistical experimental design using artificial neural network (ANN) in MATLAB 2012A was used to identify and determine the optimum process conditions. The multivariate regression analysis of the disinfection kinetic models was analyzed using SPSS version 20 and the final application of the optimized process conditions with river water was evaluated. The statistical analysis of optimization results using ANN gave a high coefficient of determination (R2) of 0.9992 and 0.9886 for E.coli. The model developed was verified and the optimum process parameters were 124 mg/L dosage, 65 minutes of contact time, 110 rpm mixing rate for E.coli bacterial strain. The order of reaction followed second order and the inactivation kinetics showed that modified Hom model was best fitted the disinfection process with R2 of 0.711. The findings from the application of the seed extract to river water showed that the removal efficiency for the seed extract with over 99.98% reduction of heterotrophic bacteria after the disinfection process. Hence, the findings of this study showed that defatted Moringa oleifera seed extract using the salt extraction method could be used as a disinfectant. This extract was recommended to be used in small communities and in emergency situations.

Genetic variants linked to T2DM risk in Kurdish populations.

Background: The polymorphisms of the C–C chemokine receptor type 5 (CCR5) and the insulin receptor substrate 1 (IRS1) have been studied as candidates for the susceptibility to develop type 2 diabetes mellitus (T2DM). CCR5 is a chemokine receptor, and the polymorphisms in the promoter region of this receptor are being studied as candidates for the susceptibility to develop T2DM. Also, IRS1 is a critical factor in the signaling pathway for insulin, and mutations in this gene have been reported, which contribute to the ability to develop T2DM. The aim of the current study was to determine the relationship between CCR5 (59029A/G) and IRS1 (rs10498210) polymorphisms with T2DM in Sanandajian patients.Methods: Genomic DNA was isolated from 200 healthy individuals and 220 Kurdish T2DM patients by salt extraction method and the polymorphisms were examined by restriction fragment length polymorphism (RFLP) method and then the results were analyzed using Chi-square test.Results: The frequency of AA genotype in 220 Kurdish patients for both genes CCR5 (OR=1.9, P=0.02) and IRS1 (OR [95% CI]=2.62, P=0.02) were significantly more than controls. There was no significant association between AG or GG genotypes in with T2DM.Conclusion: The presence of AA homozygote alleles in both loci of IRS1 (rs10498210) and CCR5 (59029A/G) genes increased the risk of T2DM.

IRS1- rs10498210 G/A and CCR5-59029 A/G polymorphisms in patients with type 2 diabetes in Kurdistan.

BackgroundThe insulin receptor substrate 1 (IRS1) is a critical factor in the signaling pathway for insulin, and mutations in this gene have been reported, which contribute to the ability to develop type 2 diabetes. The polymorphisms in the promoter region of C‐C motif chemokine receptor5 (CCR5) are also being studied as candidates for susceptibility to develop type 2 diabetes. The aim of the current study was to determine the relationship between IRS1 and CCR5 polymorphisms with type 2 diabetes in the Kurdistan population.MethodsGenomic DNA was isolated from the blood by salt extraction method and the polymorphisms were examined using Restriction Fragment Length Polymorphism (RFLP) method.ResultsThe results of current study indicated that the frequency of AA genotype in type 2 diabetic patients in both CCR5 (OR = 2.9, p = 0.04) and IRS1 (OR = 3.3, p = 0.036) were significantly more than controls.ConclusionAccording to the results of this study, the presence of AA genotype in both CCR5 and IRS1 is associated with type 2 diabetes. There was no significant association between AG or GG genotypes with type 2 diabetes.