Salivary Flow Research Articles

The wettability of complete denture base materials constructed by conventional versus digital techniques: an in-vitro study

BackgroundDecreased salivary flow can make the patients uncomfortable with their complete dentures and affects the retention of the dentures. Milling and 3D printing have become an alternative to conventional denture construction techniques. The goal of this study was to evaluate the effect of conventional and digital techniques of the complete denture construction on the denture surface wettability with distilled water and saliva substitute before and after thermocycling.MethodsA total of 30 specimens were utilized in the present study. Specimens were divided according to the construction techniques into 3 groups (n = 10 each). Group I: Heat-polymerized polymethylmethacrylate (PMMA) group, group II: Milled group, and group III: 3-dimensional (3D)-printed group. All the specimens were subjected to 2000 cycles of thermal aging in a thermocycler. The wettability of all specimens to water and saliva substitute was measured via a contact angle goniometer (Olympus TGHM, Rame-hart Inc, USA) before and after thermocycling. Descriptive statistical analysis, plots, and the Shapiro-Wilk test were used to verify normality for each variable. One-way ANOVA was used to compare the 3 study groups, while paired samples t-test was used to compare the differences within each group (P < .05).ResultsThe smallest contact angle of drop of water to the denture base specimens before and after thermocycling were recorded in the milled group (53.0 ± 4.77 and 50.27 ± 2.30, respectively), followed by the heat polymerized PMMA group (85.65 ± 4.71 and 65.06 ± 2.27, respectively), and the 3D-printed group (91.34 ± 6.74 and 90.86 ± 8.57, respectively). While the smallest contact angle of drop of saliva substitute to denture base specimens was recorded in the milled group (56.82 ± 2.29 and 34.85 ± 7.51, respectively), followed by the 3D-printed group (72.87 ± 4.83 and 58.14 ± 9.58, respectively) and the heat polymerized PMMA group (83.62 ± 4.12 and 67.82 ± 4.93, respectively). There was statistically significant difference between the groups (P < .05). A significant decline in the average contact angle of drop of saliva has been reported in all groups after thermocycling. The contact angle values differed significantly between saliva substitute and distilled water in both 3D-printed and milled groups after thermocycling (P < .001).ConclusionsThe milled denture base material presented the best wettability to water and saliva substitute than the 3D-printed and the heat-polymerized PMMA materials. Saliva substitutes improve the wetting ability of denture base materials manufactured by CAD/CAM compared with water.

Hypophysitis and central nervous system involvement in association with Sjögren’s syndrome along with hypoparathyroidism: a case report

BackgroundPatients with autoimmune diseases can develop multiple autoimmune diseases over a long period of time, and the presence of more than one autoimmune disease in a single patient is defined as polyautoimmunity. Polyautoimmunity may be clinical evidence that autoimmune diseases share similar immunological mechanisms.Case presentationWe report a 30-year-old woman with a unique combination of autoimmune diseases predominantly affecting the central nervous system, with hypoparathyroidism, hypophysitis, medulla involvement, and pons and temporal lobe involvement associated with primary Sjögren's syndrome (pSS), occurring independently over a long period. The patient who had a history of muscle cramps and one seizure incident, presented with vomiting and blurred vision. She was diagnosed with hypophysitis and hypoparathyroidism with calcifications in the basal ganglia and cerebellum. She recovered after four months of corticosteroid treatment for hypophysitis and was started on treatment for hypoparathyroidism. Eight months later, she developed vomiting, hiccups, vertigo, and ataxia with a focal lesion in the medulla. She recovered with immunosuppressive treatment for 2 years. Fifty-eight months after the onset of hypophysitis, she developed diplopia and dry mouth and eyes. MRI showed infiltrative lesions in the left pons and left temporal lobe. Based on positive anti-Sjögren's syndrome-related antigen A antibodies and low unstimulated whole salivary flow rate, pSS was diagnosed. She received corticosteroids and continued mycophenolate mofetil treatment with recovery of neurological symptoms.ConclusionThis case highlights the need for long-term follow-up to detect autoimmune disease processes involving various organs.

Salivary flow rate, subjective oral dryness and dental caries 5 years after haematopoietic cell transplantation

BackgroundThe aim of this study was to describe salivary flow rate, subjective oral dryness and dental caries 5 years post haematopoietic cell transplantation (HCT).MethodsHCT survivors of a previous longitudinal observational cohort study in the Netherlands (the H-OME study) were invited to participate in this additional follow-up after 5 years (the HOME2 study). During the additional follow-up appointment, stimulated (SWS) and unstimulated whole saliva (UWS) was collected, participants rated subjective oral dryness on a 0 – 10 scale, and caries lesions were assessed. Furthermore, dental records, including treatments and radiographs, were requested for the 5 years preceding and the 5 years following transplantation. Paired t-tests were performed to determine changes in UWS and SWS flow rates and subjective oral dryness from pre-HCT, and to compare the number of caries-related dental treatments (restorations, endodontic treatments or extractions) before and after HCT. Hyposalivation of UWS (< 0.2 mL/min) and SWS (< 0.7 mL/min) at 3 and 12 months, was used to explore the predictive potential of hyposalivation on a high dental treatment need (> 3 treatments) over the 5 years post-HCT.ResultsFive years post-HCT, 39 HCT survivors were included. The mean UWS flow rate was 0.36 mL/min (SD 0.26) and the mean SWS flow rate 1.02 (SD 0.57); survivors were diagnosed with a median of 0 dentine lesions (range 0 – 12) and 73% reported a subjective oral dryness score ≥ 1. Survivors underwent a median of 3 (range 0 – 20) dental treatments during the 5 years following transplantation. The mean difference in UWS 5 years post-HCT compared to pre-HCT was 0.03 (95% CI: -0.07 – 10.12), the mean difference for SWS was -0.18 (95% CI: -0.45 – 0.08) and for subjective oral dryness 1.2 (95% CI: 0.2 – 2.1). In the 5 years post-HCT, non-significantly more treatments were performed compared to the 5 years pre-HCT (mean difference: 0.5, 95%CI: -1.2 – 2.2). Seventy eight percent of patients with hyposalivation of SWS at 12 months had a high dental treatment need, compared with 38% with no hyposalivation.ConclusionsFive years post-HCT, mean UWS and SWS flow rates were not significantly different from pre-HCT levels but subjective oral dryness scores were elevated.

Dental caries status and treatment need among pregnant women attending antenatal clinics in Dar-Es-Salaam region, Tanzania

BackgroundLiterature shows that pregnant women are more susceptible to dental caries due to several reasons, including changes in salivary flow, reduced pH in the oral cavity, and sugary dietary cravings. The unmet need for care is of special concern, as the failure to obtain treatment can affect the health status of the mother and her unborn child.ObjectivesTo determine dental caries status and treatment needs among pregnant women attending antenatal clinics in the Dar-es-Salaam region.Materials and methodsA descriptive cross-sectional hospital-based study was conducted among 461 pregnant women aged 14–47 years. Informed consent was obtained from participants during data collection. Dental caries status and treatment need were diagnosed according to the WHO criteria. The Data collected was cleaned and analyzed using SPSS version 23.0 software for generating frequency distribution tables, chi-square tests, and logistic regression analysis. The Confidence Interval was 95% (p < 0.05).ResultsThe overall prevalence of dental caries was 69%, with a mean DMFT score of 2.86 (± 3.39). Untreated dental decay was observed in 60.5% of study participants, which needed more restorative treatment (fillings & RCT) than a tooth extraction. Caries experiences differed significantly among the pregnant women in various gravidity. In logistic regression, dental caries experience was significantly higher among multigravida respondents (p-values = 0.04) (OR: 1.840, CI 1.021–3.319).ConclusionThis study demonstrated a high level of dental caries experience and the presence of treatment needs. Multiple pregnancies were a major factor that contributed to high levels of dental caries.

Impact of salivary flow inhibition on masticatory behaviours in healthy individuals.

It remains unclear how the salivary flow and the fat content of food affect bolus formation during mastication. We aimed to clarify: (1) how hyposalivation affects jaw-closing and hyoid-elevating muscle activities in bolus formation, and (2) if the effect of hyposalivation on muscle activity depends on the fat content of food. Eighteen healthy male volunteers were instructed to freely ingest four test foods: Plain, Fat without seasoning, Fat with seasoning, and Soft rice crackers. Masseter and suprahyoid electromyographic activities were recorded before and 30 min after the administration of atropine sulfate, a muscarinic receptor antagonist that induces hyposalivation. Hyposalivation extended the masticatory duration significantly in all the test foods except Fat with seasoning. Masticatory cycle time was significantly longer with vs without hyposalivation for the Soft (p = .011). Suprahyoid activity/cycle was significantly greater with vs without hyposalivation (p = .013). Masticatory cycle time was significantly longer at the late stage with vs without hyposalivation for the Soft (p < .001). Suprahyoid activity/cycle was significantly greater at the middle (p = .045) and late stages (p = .002) with vs without hyposalivation for the Soft and greater at the late stage with vs without hyposalivation for the Plain (p = .043). Changes in masticatory cycle time and suprahyoid activity/cycle for these foods had significantly positive relationship (p < .001). Hyposalivation-induced changes in masticatory behaviours resulted from the middle and late stage suprahyoid activity. Fat content and seasoning compensate for salivary flow inhibition.

Caries Risk Assessment in Adults with Highlights to Nutrigenomics and Nutrigenetics

Abstract Caries risk assessment and caries management by risk assessment cannot be related only to children and their parents' answers. Camra and Cariogram have been working for decades in few countries. Prediction and preventive measures are especially important for young adults (16-25 years old), young professionals (25-35 years old), before pregnancy and during menopause. Aim To develop a card for an easy caries risk assessment with chair-aside questions, clinical examination and salivary tests. To draw up a protocol for patient treatment and follow-up related to the caries risk. Methodology It is based on the data of 1,167 participants in 6 different clinical studies conducted within various research trials, granted programmes and projects during the 30-year period from 1988 to 2018. Prediction of risk can be useful if 4 groups, i.e. low risk, moderate risk, high risk and very high risk groups are evaluated. Results Packages of measures were targeted for each group. Among the important factors were the type, habits and frequency of nutrition, detected with 6 targeted questions, lifestyle hygiene with 5 questions, dental status with 6 parameters (DMFT scores, gingival status, size of teeth, etc.) and oral hygiene. Biochemical patterns of saliva, i.e. salivary flow rate and pH, with and without stimulation were included. Low risk and Moderate risk groups of different ages do not need further tests. High risk and Very high risk groups can be additionally tested with chair-aside tests for Streptococcus mutans and Lactobacilli counts and oral sucrose clearance for preventive evaluation of dental caries management. The card validation and tests last no longer than 40 minutes, 1 page for a single patient. Conclusions Caries risk in adults can be measured, assessed and predicted in relation to nutrigenomics. Treatment behaviour can be managed and targeted for each patient, according to the group for long, lifetime saving of natural teeth in the mouth. In the last 100 years, concentrated and refined foods have decreased all sizes of the upper first and second incisors as a natural physiological response and a change has been observed in the anatomy of canines, making them stronger via enlargement of their MD sizes. From this current academic year, "Caries risk" is included into our new lecture programme.

An Overview of Prosthetic Implant Attachments in EdentulousPatients

Edentulism is an unresolved health care issue of sustained significance and prevalence in the aged population. Adaptation to wearing complete dentures is a complex process and must be considered from both somatic and psychological standpoints. The difficulties of wearing dentures have been attributed to deficiencies of the denture-bearing tissues, reduced salivary flow,vulnerable tissue and severe ridge resorption. The prosthetic management of the edentulous patient has always been a major challenge. Complete maxillary and mandibular dentures have been the traditional treatment option. However, most of the patients report problems adapting to the mandibular denture due to lack of comfort, retention and stability. It is well known that implantretained/supported overdentures provide improved retention, support, stability, function, and comfort for patients

The effect of using the CAMBRA mobile application on dental caries management in children and adolescents

Objectives: This study aimed to evaluate the effectiveness of the CAMBRA-students application by assessing the changes in dental caries risk factors and management effectiveness in elementary and middle school students. Methods: This study was conducted between July 2023 and May 2024 and included 113 participants (55 experimental and 58 control participants). Participants used the CAMBRA-students application to enter the protective and risk factors. Clinicians recorded the disease indicators and classified the participants into risk groups. Only the experimental group received the tailored dental caries management program, with feedback provided via the applicationat each intervention stage. Results: The pre- and postintervention assessments revealed an increase in the high-risk group and a decrease in the extremehigh-risk group. Conversely, the control group exhibited a decrease in the high-risk group and an increase in the extreme high-risk group. The DMFT index and rateand salivary flow rate increased in both groups. The Simple Hygiene Score and ΔR decreased and increased in the experimental and control groups, respectively, with no statistically significant differences. Conclusions: The CAMBRA-students application effectively prevented dental caries in children and adolescents. The future development of personalized oral care programs tailored to different life stages is recommended.

Efficacy of BAFF inhibition and B-cell depletion in non-obese diabetic mice as a spontaneous model for Sjögren’s disease

IntroductionThe therapeutic interest of targeting B-cell activating factor (BAFF) in Sjögren’s disease (SjD) can be suspected from the results of two phase II clinical trials but has not been evaluated...

Contribution of kelp dashi liquid to sustainable maintenance of taste sensation and promotion of healthy eating in older adults throughout the umami-taste salivary reflex.

Taste decline, including taste loss in older adults, leads to malnutrition and frailty. In a super-aging society, improving taste decline and maintaining taste sensation are crucial for the wellbeing of older adults. Hyposalivation frequently affects older individuals and is the leading cause of taste decline in older adults. Treating taste decline, including taste loss, in older adults presents challenges due to the limited sustainable methods for increasing saliva production, except for drug therapy, which may lead to adverse effects. Umami-taste stimulation results in a prolonged increase in both the whole salivary flow rate (WF), more than 90% of which is secreted from the major salivary glands, and the minor salivary gland flow rate (MF) in healthy volunteers through the umami-taste salivary reflex. We hypothesized that umami-rich kelp dashi liquid (KDL), commonly used in Japanese cuisine, may alleviate taste decline and sustain normal taste sensation in older adults with hyposalivation. This study investigated whether KDL stimulation could improve taste decline. A non-randomized controlled trial was conducted at the dental department of a university hospital, involving those who presented with dry mouth between May 2017 and December 2021. Before and after repeated KDL stimulation, characteristics like changes in WF and MF, the recognition thresholds (RTs) for five basic tastes, and subjective eating and swallowing difficulties were assessed. Statistical comparisons were performed between the values measured before and after KDL stimulation. A total of 35 older patients were included. Patients with reduced MF and with or without reduced WF exhibited umami-taste loss. Repeated stimulation with KDL increased MF and WF and improved taste loss, including umami, decreased RTs, and normalized each taste. Furthermore, subjective taste impairment, subjective eating and swallowing difficulties, and burning sensations in the oral mucosa were alleviated. These findings indicate that KDL stimulation improved umami-taste loss and normalized each taste sensation, further alleviating eating difficulties via the umami-taste salivary reflex. Importantly, umami-taste loss was also observed in patients with normal WF but decreased MF, who are typically not diagnosed with hyposalivation. Therefore, KDL has the potential to sustain taste sensations and promote healthy eating habits in older individuals.

Comparative Evaluation of Hearing and Salivary Flow Rate in Smokers and Non-smokers: A Cross-Sectional Study.

Background Smoking is a major global health issue that is linked to varioushealth conditions, including hearing loss and reduced salivary flow. This study aims to explore the relationship between smoking, hearing loss, and salivary flow rate. Methods This cross-sectional study was conducted over two months at a tertiary healthcare institute in Central India, involving 100 participants (50 smokers and 50 non-smokers) aged 18-55 years. Hearing status was assessed using audiometry, and the salivary flow rate was measured. Statistical analyses were performed using SPSS version 21 (IBM Corp., Armonk, NY, US). Results Smokers had a significantly higher prevalence of hearing loss (40%) compared to non-smokers (10%). The salivary flow rate was significantly lower in smokers (mean 0.5540 ml/min) than in non-smokers (mean 0.9240 ml/min). However, no significant correlation was found between the duration and frequency of smoking with hearing loss or salivary flow rate. Conclusion Smoking significantly impacts both hearing and salivary flow rate, hence smokers show a higher risk of hearing loss and reduced salivary flow rate. Early hearing screenings and preventive measures are recommended for smokers. Further research with larger sample sizes is needed to explore the long-term effects of smoking on these health parameters.

Assessment of Chronic Malnutrition and Its Correlation With Oral Health Status in Children Aged Three to Six Years in Jabalpur District, India: An Epidemiological Study.

This study aims to examine the correlation of chronic malnutrition with the oral health status of children aged three to six years. A total of 400 children were selected and divided into four groups based on z-scores. For evaluation of oral health status, teeth were examined for dental caries using the decayed, missing, and filled permanent teeth (DMFT) scores. Salivary samples were collected to measure salivary flow rate (SFR), pH, and salivary buffering capacity (SBC). Teeth were also visualized to detect the presence or absence of enamel hypoplasia. The data obtained was statistically analyzed (p<0.05). A total of 400 children participated and were also categorized into mild, moderate, and severe obesity according to the z-scores. The mean DMFT scores among adequately nourished children were 2.4086; with severely malnourished and severely obese children, the mean values were found to be 1.0652 and 1.4286, respectively. The mean SFR among children with adequate nutrition was 1.0366, and the mean flow rate among children with severe malnutrition and obesity was 0.5348 and 0.4036, respectively. The mean salivary pH among children with adequate nutrition was 7.1295, and the mean values for participants with severe malnutrition and severe obesity were found to be 6.4772 and 7.6521, respectively. The mean SBC among children with adequate nutrition was found to be 4.4861; for severely malnourished children and those with severe obesity, the values were 3.2472 and 2.8332, respectively. There was an absence of enamel hypoplasia in children with adequate nutrition, whereas a total of five participants with severe malnutrition and three children with severe obesity were found to have hypoplastic lesions, respectively. Malnutrition exerts a negative impact on the overall oral health of children. It is critical to diagnose the effects of malnutrition on children's oral environments in order to provide appropriate treatment and enhance their quality of life.

Effect of adiposity status on salivary Streptococcus mutans and Streptococcus sobrinus.

To investigate the effect of adiposity status, salivary physicochemical parameters, and inflammatory biomarkers on the salivary abundance of the two main cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus among Hong Kong adolescents. This study included 180 adolescents aged 12-15 years from Hong Kong local secondary schools. Anthropometric measurements and oral health examinations were performed. Saliva samples were collected to measure salivary physicochemical parameters, protein biomarker levels, and salivary abundance of Streptococcus mutans, Streptococcus sobrinus, and total bacteria. Adolescents with general overweight/obesity or central obesity had significantly higher salivary Streptococcus mutans abundance and total bacterial load, lower stimulated salivary flow rate, and lower secretory phospholipase A2 group IIA levels than the lean controls. Adolescents with general overweight/obesity (OR = 3.33; 95% CI: 1.28-8.65) and those with central obesity (OR = 2.47; 95% CI: 1.02-6.00) had a significantly higher chance of having high salivary abundance of Streptococcus mutans than the lean controls after adjusting for confounders, with a similar detection rate of salivary Streptococcus sobrinus. General overweight or obesity and central obesity were associated with a high salivary abundance of Streptococcus mutans but had no significant effect on salivary Streptococcus sobrinus.

Untargeted stimulated and unstimulated salivary metabolomics and saliva flow rate in children.

The present study aimed to determine the salivary flow and metabolomic profile of stimulated and unstimulated saliva in children. Children who attended the Pediatric Dentistry Clinic of the State University of Rio de Janeiro -UERJ between 3 and 12 years of age were selected. Unstimulated and stimulated whole saliva, using mechanical stimulus, were collected. The samples were centrifuged at 12,000 g, 4oC for 1h. The 1H- NMR spectra were acquired in 500MHz equipment. The data were extracted into 0.03 ppm buckets in AMIX, and multivariate analysis (PLS-DA and O-PLS-DA) was performed in Metaboanalyst 2.0. For other analyses, such as salivary flow, the data was tabulated in the SPSS 20.0 statistical package, analyzed descriptively, and after applying the Wilcoxon test. The interval of confidence was set at 95%. The mean age was 7.5 (± 1.94), and 47.0% (n = 31) were female, 63.6% (n = 42). The median flow rate for stimulated saliva was 0.74 (IC 0.10-2.40) and was statistically higher (p < 0.001; Wilcoxon test) than unstimulated was 0.39 (IC 0.00-1.80). Children older than seven years old also presented a higher difference between unstimulated and stimulated saliva (p = 0.003; Mann-Whitney test). The PLS-DA and O-PLS-DA demonstrated a different profile in stimulated and unstimulated saliva. Acetate, glucose, propionate, and lysine were higher in the unstimulated whole saliva than in stimulated saliva. Isoleucine, N-acetyl sugar, hydroxybutyrate, glutamate, leucine, propionate, butyrate, valine, isoleucine, succinate, saturated fatty acid, and histidine were found in greater amounts in the saliva of patients with stimulated saliva. The stimulated saliva presented a higher flow rate, and older children exhibited a higher flow rate resulting from it's the stimulus. The mechanical stimulus increased the levels of the major metabolites.

Oral health-related quality of life in Fanconi anemia: a cross-sectional study.

To evaluate the oral health-related quality of life (OHRQoL) of individuals diagnosed with Fanconi anemia (FA). A cross-sectional study was conducted with FA patients from two Brazilian referral centers. Participants underwent a complete dental, periodontal, and oral mucosa examination, as well as assessment of resting salivary flow. The short version of the Oral Health Impact Profile (OHIP-14) questionnaire was administered. Descriptive and bivariate analyses were performed, followed by multivariate analysis to examine the impact of independent variables on OHRQoL. The study included 20 (57.1%) males and 15 (42.9%) females, with a mean age of 18.9years. Oral leukoplakia (OL) was found in 18 individuals. The overall OHIP-14 score was 9.9 ± 10.5. Individuals aged ≥ 16years had higher OHIP-14 scores, indicating worse OHRQoL for physical pain (p = 0.007), psychological discomfort (p = 0.001), physical disability (p = 0.03), psychological disability (p = 0.001), handicap (p = 0.004), and overall score (p = 0.007). Females reported more negative OHRQoL than males for physical pain (p = 0.02), psychological discomfort (p = 0.03), psychological disability (p = 0.009), and overall score (p = 0.02). Individuals with OL had an overall OHIP-14 score 1.83 times higher than those without OL (95% CI: 1.02-3.28; p = 0.04). Lower salivary flow correlated with higher overall OHIP-14 scores (95% CI: 0.14-0.84; p = 0.01). This study represents the first attempt to evaluate OHRQoL in individuals with FA. The presence of OL and reduced salivary flow were identified as predictors of a negative impact on OHRQoL. It is imperative to integrate patients' quality of life in the clinical treatment protocols for the FA population.

Exploring the impact of bubble type on sensory stimulation in drinks

This study explores the impact of various types of carbonation on sensory stimulation in the mouth, salivary secretion and the neurotransmitter substance P (SP), as well as body responses such as heart rate (HR) and Galvanic Skin Response (GSR). Three types of carbonation (one made using a soda machine, another carbonated with a gasifier, and the last commercial sparkling water) were used to produce different bubbles resulting in distinct sensory characteristics assessed by a trained panel. The impact of carbonation was measured by recording changes in salivary flow rate, SP levels, salivary secretory immunoglobulin A (SIgA), HR, and GSR in fifteen healthy participants. The results showed that the bubble type only affected the sensory perception of carbonation. Regardless of bubble type, carbonation increased salivary flow rate and SP values, SigA and HR. These characteristics are being sought to improve treatments for dysphagia or dry mouth. Therefore, these findings highlight the potential therapeutic application of carbonation in these situations.

Remineralization of post-orthodontic white spot lesions with a fluoride varnish and a self-assembling P 11 - 4 peptides: a prospective in-vivo-study.

The objective was to evaluate the remineralization effects of fluoride varnish (Clinpro White varnish), self-assembling peptide (Curodont™ Repair) and their combined use on WSL after orthodontic treatment. Thirty-two subjects, aged of 10-18 (mean age 13.91 ± 2.92) with 107 post-orthodontic WSL were included in the study. Subjects were divided into four groups as control, tricalcium phosphate (TCP) containing fluoride varnish (Clinpro White varnish) group, self-assembling P11-4 peptides (Curodont™ Repair) group and combined application of the two products. At the beginning, each subjects' caries risk profile was assessed by evaluating diet cariogenicity, plaque index, gingival bleeding index and stimulated salivary flow rate. Before the application of the remineralization agents, WSL baseline demineralization values were determined with QLF Inspektor™ Pro, laser fluorescence using DIAGNOdent and color values were measured by Vita EasyShade. Remineralization data were obtained by measuring ΔF, ΔQ, and lesion area with QLF. The aesthetic improvement after the remineralization process was evaluated with a spectrophotometer at six weeks, three and six months. No statistically significant differences were found between the groups in terms of criteria determining patients' caries risk profiles, DIAGNOdent data, and plaque index scores (p > 0.05). Intra-group evaluation following remineralization revealed statistically significant increases in ΔF and ΔQ with a decrease in lesion area for the fluoride varnish group at six months, for the peptide group at three months, and for the combined application group at three and six months (p < 0.05). In inter-group comparisons, ΔF and ΔQ values were found to be statistically significant only in the fluoride group at six months compared to the other groups (p < 0.05). While the L* value decreased significantly in all groups at six months, a statistically significant difference in ΔE* values was observed only in the control group between three and six months. Fluoride varnish with TCP showed highest remineralization at 6 months, and the remineralization was positively affected in the short term (three months) after the use of self-assembling P11-4 peptides and their combined application. Remineralization obtained after single application of agents tested in six months in-vivo showed parallel results. In an attempt to trigger subsurface remineralization, the combined use of fluoride with self-assembling peptides as biomimetic remineralization agent needs further evaluation.

Salivary Profile Analysis Based on Oral Cancer Risk Habits: An Observational Cross-Sectional Study.

In Indonesia, cultural practices such as betel quid chewing, smoking, and alcohol consumption are prevalent. These practices are known risk factors for oral cancer and may influence the salivary profile, which is essential for maintaining oral health. To compare the salivary profiles of individuals with and without risk factors for oral cancer. The study included 49 individuals identified as having risk factors for oral cancer. Unstimulated saliva samples were collected. Various parameters were measured, including salivary pH, flow rate (FR), thickness, color, turbidity, and the levels of IL-1β and IL-8. Data were analyzed using Chi-square and t-tests. A significant difference was found in salivary IL-1β levels between the two groups (p = 0.009), with higher levels observed in individuals with oral cancer risk factors. Notably, the salivary IL-1β concentrations showed significant differences between the smoking group (p = 0.021; OR = 2.94) and the alcohol-drinking group (p = 0.007; OR = 4.96) compared to the control group. However, no significant differences were observed between the groups in terms of salivary viscosity, color, turbidity, flow rate, acidity, or IL-8 levels (p > 0.05). Individuals with risk factors for oral cancer exhibit distinct salivary IL-1β profiles compared to those without such risk factors, particularly those who practice alcohol drinking.

Clinical factors associated with salivary flow rate in adults with acromegaly

Objective. To investigate the stimulated salivary flow (SSF) and unstimulated salivary flow (USF) in adults with acromegaly and to identify possible clinical factors associated with salivary flow. Study design. A case-control study was conducted with a group composed of adults diagnosed with acromegaly (n=29, mean age =50.2 years) and a control group (n=29, mean age =54.3 years). Variables for socio-demographic characterisation, lifestyle habits, and diabetes diagnosis, body mass index (BMI), cervical circumference (CC) and abdominal circumference (AC) were collected. USF and SSF variables were analysed as outcomes. Unpaired t-test, Pearson's correlation, and multivariate regression models were used for statistical analysis. Results. Both groups were 44.8% male and 55.2% female. Diabetes was present in 55.2% of the acromegaly group and in 51.7% of the controls (P = 0.792). The acromegaly group had a higher USF than the control group (0.50 ml/min versus 0.22 ml/min). SSF showed a direct correlation with CC (r = 0.470, P=0.010). Acromegaly was associated with higher USF (standardised coefficient = 0.780, P=0.039), and age was inversely related to USF (standardised coefficient = -0.333, P=0.013). Conclusions. The findings suggest that adults with acromegaly have an increased USF and that being older is associated with a decrease in USF.

To assess the effect of selected locally available beverages on salivary pH, flow rate, and oral clearance rate among adults

Aim: The aim of this study is to assess the effect of selected locally available beverages on salivary pH, flow rate, and oral clearance rate among adults. Materials and Methods: A quantitative diagnostic method for dental caries would improve oral health, which directly affects the quality of life. The pH scale measures how acidic or basic a solution is, with 7 being neutral. When the pH of plaque drops below normal, or &lt;5.5, the acidity starts to dissolve minerals and destroy the tooth’s enamel. This clinical trial will be conducted on 40 subjects. Test beverages will be Red Bull, mixed fruit juice, tea, and sweetened milk. Unstimulated salivary samples will be collected for each study subject at least 1 h after their breakfast. After the collection of baseline salivary samples, the subjects will be given one beverage to drink, and then stimulated saliva samples will be collected at fixed time intervals. A digital pH meter will be used to measure the pH at every step. Statistical Analysis: This will be carried out using SPSS version 17, one-way ANOVA, and post hoc Tukey’s test will be applied in the statistical tests. Results: The maximum drop in pH was seen in the case of carbonated beverage (Red Bull), the oral clearance for all the liquid food items was achieved at 14 minutes. Conclusions: Liquids had a considerable cariogenic and erosive potential despite their quick clearance from the mouth cavity. it is always recommended to limit beverage consumption to maintain good dental health.