Saline-alkali Soil Research Articles

Effects of synergistic phosphate fertilizer on photosynthetic characteristics and senescence of wheat flag leaf in saline-alkali soil

Effects of synergistic phosphate fertilizer on photosynthetic characteristics and senescence of wheat flag leaf in saline-alkali soil

A Review on the Improvement of Saline-alkali Soil by Organic Fertilizer

In recent years, soil salinization has become a serious problem in the global environment. The application of organic fertilizer to improve saline-alkali soil has been widely concerned because of its advantages of improving soil fertility, improving soil structure and improving soil salt ion composition. This paper reviews the mechanism of saline-alkali soil improvement, and expounds the improvement prospect of organic fertilizer on saline-alkali soil from the influence of organic fertilizer on soil physical and chemical properties, water and salt transport law, crop yield and quality, in order to provide theoretical guidance for saline-alkali soil restoration.

Enhanced Soil Carbon Stability through Alterations in Components of Particulate and Mineral-Associated Organic Matter in Reclaimed Saline–Alkali Drainage Ditches

Soil carbon content and stability are primarily influenced by the stabilization of particulate organic matter (POM) and mineral-associated organic matter (MAOM). Despite extensive research on the stabilization processes of POM and MAOM carbon components under various land-use types, the investigation into stabilization processes of soil carbon remains limited in saline–alkali soils. Therefore, we collected soil samples from different positions of saline–alkali drainage ditches at four reclamation times (the first, seventh, fifteenth, and thirtieth year) to determine their carbon content and physicochemical properties. Moreover, POM and MAOM fractions were separated from soil samples, and Fourier transform infrared spectra (FTIR) were used to investigate changes in their chemical composition. The results showed that with increasing reclamation time, the soil total carbon and soil organic carbon (SOC) contents significantly increased from 14 to 15 and 2.9 to 5.5 g kg−1, respectively. In contrast, soil inorganic carbon content significantly decreased from 11 to 9.6 g kg−1. Notably, the changes in soil carbon components following the increasing reclamation time were primarily observed in the furrow sole at a depth of 20–40 cm. While the SOC content of the POM fraction (SOCPOM) decreased significantly, the SOC content of the MAOM fraction (SOCMAOM) increased significantly. These alterations were largely dominated by drainage processes after reclamation instead of a possible conversion from SOCPOM to SOCMAOM. FTIR results revealed that MAOM was greatly influenced by the reclamation time more than POM was, but the change in both POM and MAOM contributed to an increase in soil carbon stability. Our findings will deepen the comprehension of soil carbon stabilization processes in saline–alkali drainage ditches after reclamation and offer a research framework to investigate the stability processes of soil carbon components via alterations in POM and MAOM fractions.

Phosphorus fractions affect fungal compositions and functions under land use conversions in saline-alkali soil in northeastern China

BackgroundLittle is known about the link between the fungal community and phosphorus fractions when land use is converted from cropland to grassland in saline-alkali soil in northeastern China. Therefore, in this study, the diversity, composition, and function of fungi, as well as phosphorus fractions including Olsen-P and inorganic phosphorus (Pi), were investigated under land use conversions from maize cropland (MC) to alfalfa (Medicago sativa L.) (AG), Leymus chinensis (LG), and natural restored grasslands (RG).ResultsThe results showed that the Pi fractions of Ca8-P, Fe-P, Ca2-P, and Ca10-P were closely related with Olsen-P. Significantly increased Olsen-P content was found in 0–10 cm soil layer in the AG treatment, relative to LG and RG treatments (P < 0.05). The occluded P content in 0–10 cm and the Al-P content in 10–20 cm in the RG treatment were the highest. The RG treatment increased the Shannon index of fungi, as well as the abundances of phyla Mortierellomycota and phyla Glomeromycota. Higher abundance of genus Mortierella and lower abundance of genus Cladosporium were observed at RG treatment. Moreover, the RG treatment greatly reduced the abundance of plant pathogens and enhanced the abundances of mycorrhizal and ectomycorrhizal. The Olsen-P was positively correlated with the abundance of plant pathogen (P < 0.01), and the Olsen-P, Ca2-P, and Fe–P were negatively correlated with both the abundances of mycorrhizal and ectomycorrhizal (P < 0.05).ConclusionLand use conversion from maize cropland to natural restored grassland could reduce plant pathogens and enhance useful fungi by decreasing the availability of phosphorus.Graphical

Effect of Lithium Slag Application on Saline–Alkali Soil Amelioration and Vegetable Growth

Increased attention has been attracted to saline–alkali soil amelioration due to the growing serious salinization of soils in the world. Lithium slag (LS) is an acid by-product of lithium production with potential properties to ameliorate alkalinity in saline–alkali soils. In this study, LS was reused as a saline–alkali soil amendment and potted plant experiments in a greenhouse were performed to evaluate the effect of LS application on the soil amelioration and the growth of vegetables (roquette and radish) in the saline–alkali soil during the 5-week growth period. LS was added at the amount of 0.5%, 1.0%, 2.0%, 5.0%, 8.0% and 10.0% (w/w) levels. Results showed that saline–alkali soil pH dropped obviously with the increase in LS application. Accordingly, the germination, survival and growth of roquette and radish were significantly improved by LS addition, especially at the optimum amount of 0.5% and 1.0% (w/w) in the saline–alkali soil. In contrast to the untreated saline–alkali soil, LS addition at 0.5% and 1.0% (w/w) levels increased the roquette’s height by 49.7% and 36.1% and increased the radish’s height by 54.6% and 53.7%, respectively. However, the soil electrical conductivity (EC) and soluble salt content increased with the addition of LS, and the salt stress induced by excessive LS (over 5.0% level) could inhibit the growth of plants. This study proposes a new way for the effective application of LS in the amelioration of saline–alkali soil in order to realize environment and resource sustainability.

Enhancing the growth performance of Sesbania cannabina using Ensifer alkalisoli and biochar under salt stress

Sesbania cannabina is a leguminous salt-tolerant plant that has been effectively used in saline-alkaline land restoration, and forms symbiotic interactions with various rhizobia to form nodules. Ensifer alkalisoli YIC4027, a rhizobium, was screened from S. cannabina root nodules and has significant host specificity. However, the mechanism underlying the symbiotic salt tolerance of S. cannabina -YIC4027, and strategies to enhance this tolerance remain poorly understood. In this study, the mechanism underlying the effect of YIC4027 on the salt tolerance of S. cannabina and the effect of straw biochar on the symbiotic nodulation of S. cannabina-YIC4027 under salt stress were analyzed using a vermiculite pot test. The results indicated that inoculation with YIC4027 markedly increased the biomass, chlorophyll content, photosynthetic rate, superoxide dismutase (SOD) activity and catalase (CAT) activity of S. cannabina under salt stress, while there was no obvious change in glutathione (GSH) or proline (PRO) content. The nitrogen supply and salt concentration are important regulators of YIC4027 nodulation. Salt stress reduced the nodulation efficiency of YIC4027 by 66.67%, and straw biochar application resulted in a 5-fold increase in nodulation efficiency of YIC4027. The present results further suggest that the combination of YIC4027 and straw biochar is an effective biological method for enhancing the effectiveness of S. cannabina in saline-alkali soil improvement.

Peroxidase like Zn doped Prussian blue facilitates salinity tolerance in winter wheat through seed dressing

Salt stress severely limits the growth and yield of wheat in saline-alkali soil. While nanozymes have shown promise in mitigating abiotic stress by scavenging reactive oxygen species (ROS) in plants, their application in alleviating salt stress for wheat is still limited. This study synthesized a highly active nanozyme catalyst known as ZnPB (Zn-modified Prussian blue) to improve the yield and quality of wheat in saline soil. According to the Michaelis-Menten equation, ZnPB demonstrates exceptional peroxidase-like enzymatic activity, thereby mitigating oxidative damage caused by salt stress. Additionally, studies have shown that the ZnPB nanozyme is capable of regulating intracellular Na+ efflux and K+ retention in wheat, resulting in a decrease in proline and soluble protein levels while maintaining the integrity of macromolecules within the cell. Consequently, field experiments demonstrated that the ZnPB nanozyme increased winter wheat yield by 12.15 %, while also significantly enhancing its nutritional quality. This research offers a promising approach to improving the salinity tolerance of wheat, while also providing insights into its practical application.

Core bacterial communities dominated Purus frumentum biomass under different green manure returning amounts in saline-alkali soil

Core bacterial communities dominated Purus frumentum biomass under different green manure returning amounts in saline-alkali soil

Short-term high-temperature pretreated compost increases its application value by altering key bacteria phenotypes

Short-term high-temperature pretreatment can effectively shorten the maturity period of organic waste composting and improve the fertilizer efficiency and humification degree of products. To investigate the effect and mechanism of the end products on the saline–alkali soil improvement and plant growth, the short-term high-temperature pretreatment composting (SHC) and traditional composting (STC) were separately blended with saline–alkali soil in a ratio of 0–40 % to establish a soil–fertilizer blended matrix for cultivating Lolium perenne L. The pot experiments combined with principal component analysis showed Lolium perenne L. planted in 20 % SHC-blended saline–alkali soil had the best growth effect, and its biomass, chlorophyll content, and plant height were 109–113 % higher than STC. The soil physicochemical property analysis showed that SHC and STC increased the soil nutrient content, humification degree, and enzyme activity at any blending ratio. The microbial analysis showed that 20 % SHC in the saline–alkali soil stimulated the growth of functional microorganisms and the addition of SHC promoted the sulfur cycle, nitrogen fixation, and carbon metabolism in the soil–plant system. The correlation analysis showed that pH; nutrient contents; and urease, catalase, sucrase, and phosphatase activities in the saline–alkali soil were significantly correlated with plant growth indexes (p < 0.05). Georgenia and norank_f__Fodinicurvataceae had a stronger correlation with four types of enzyme activities (p < 0.01). SHC improved the saline–alkali soil and promoted plant growth by adjusting soil pH, increasing soil nutrients, and influencing soil enzyme activity and dominant flora. This study provides a theoretical basis for applying SHC products in soil improvement.

Phycoremediation Potential of Salt-Tolerant Microalgal Species: Motion, Metabolic Characteristics, and Their Application for Saline-Alkali Soil Improvement in Eco-Farms.

Microalgae have great potential for remediating salt-affected soil. In this study, the microalgae species Coelastrella sp. SDEC-28, Dunaliella salina SDEC-36, and Spirulina subsalsa FACHB-351 were investigated for their potential to rehabilitate salt-affected soils. Nylon screens with optimal aperture sizes and layer numbers were identified to efficiently intercept and harvest biomass, suggesting a correlation between underflow capability and the tough cell walls, strong motility, and intertwining characteristics of the algae. Our investigations proved the feasibility of incorporating monosodium glutamate residue (MSGR) into soil extracts at dilution ratios of 1/200, 1/2000, and 1/500 to serve as the optimal medium for the three microalgae species, respectively. After one growth period of these three species, the electrical conductivities of the media decreased by 0.21, 1.18, and 1.78 mS/cm, respectively, and the pH remained stable at 7.7, 8.6, and 8.4. The hypotheses that microalgae can remediate soil and return profits have been verified through theoretical calculations, demonstrating the potential of employing specific microalgal strains to enhance soil conditions in eco-farms, thereby broadening the range of crops that can be cultivated, including those that are intolerant to saline-alkali environments.

Gel-Embedded Biochar and Hydroxyapatite Composite for the Improvement of Saline-Alkali Soil and Plant Growth Promotion.

Soil amendments play a crucial role in modern agriculture, as they effectively enhance the planting environment. This study innovatively proposes the use of gel as a crosslinking agent to embed biochar and hydroxyapatite (HAP), thereby preparing a novel soil amendment. Furthermore, this study investigates the soil improvement effects of this amendment as well as its influence on plant growth. This study employed a hydrothermal method to combine corn stalk (CB) or sludge (SB) biochar with HAP at different ratios (0-20%). Subsequently, sodium alginate gel (SA) was utilized to encapsulate the biochar and minerals, successfully forming a ternary composite gel material (corn stalk biochar/sludge biochar-sodium alginate gel-hydroxyapatite: CB/SB-SA-HAP). Finally, the practical effectiveness of this amendment was verified through potted soil experiments. The results indicate that the CB/SB-SA-HAP composite materials exhibited a micrometre-scale spherical structure with well-developed micropores and possess the functional groups of CB/SB, SA, and HAP, along with unique mineral properties. Through pot experiments, it was verified that the composite material effectively enhances multiple soil properties. After 21 days of cultivation, the soil pH values stabilized within the neutral range (pH = 7 ± 0.3) across all treatment groups. Except for the CB0 (CB:HAP = 1:0) and CB2.0 (CB:HAP = 1:2) treatments, the remaining treatments significantly reduced the soil EC values by 3.27% to 47.92%. All treatments significantly increased the contents of alkali-hydrolysable nitrogen (AHN) (34.89~57.91%), available phosphorus (AP) (35.93~56.55%), and available potassium (AK) (36.41~56.80%) in the soil. In comparison, although the SB treatment was more effective in regulating the pH and electrical conductivity (EC) of saline-alkali soil than the CB treatment, it was less effective in promoting plant growth in the short term. Through correlation analysis and redundancy analysis, a significant positive correlation was found between soil pH and ryegrass germination rate and plant height, particularly with the most pronounced impact on soil pH observed in the CB1.0 and SB0 (SB:HAP = 1:0) treatments. This study underscores the potential of CB/SB-SA-HAP composite materials in soil improvement and plant growth promotion, providing valuable insights for soil remediation, enhancement, and plant cultivation advancements in the agricultural sector.

Optimizing Biochar Application Rates to Improve Soil Properties and Crop Growth in Saline–Alkali Soil

There is great demand for the amelioration of saline–alkali soils, which requires efficient and economical amendments. Biochar addition could alleviate the adverse impacts of saline–alkali stress in crops. However, their efficiency and optimal amounts in saline–alkali soil restoration remain contradictory and inconclusive. The objective of this study was to investigate the effects of biochar application on the properties of saline–alkali soil and crop growth, as well as to determine the optimal application rate of biochar. We conducted pot experiments with biochar (B) application rates, including 0 (CK), 1% (B-1%), 2.5% (B-2.5%), 5% (B-5%), and 10% (B-10%), studying the impact of biochar on soil water content (SWC), soil salinity, soil electrical conductivity (EC), soil ion content, soil nutrients, soil enzyme activity, and crop growth. A four-parameter Gaussian function was established for the curves depicting the relationship between soil salinity characteristics and the biochar application rates to determine the most optimal application rate. The results indicated that: (1) Compared to the CK, all biochar treatments improved soil water-holding capacity and reduced soil Na+ content and sodium adsorption ratio (SAR). (2) B-1%, B-2.5%, and B-5% treatments reduced soil content, EC, Cl−, and SO42− content over CK, while the results were reversed for the B-10% treatment. (3) Compared to the CK, all biochar treatments significantly increased soil fertility, enhanced soil enzyme activity (alkaline phosphatase, catalase, and urease activity), and significantly promoted the growth of maize. (4) The results of the Gaussian model suggested that a biochar application rate of 3.16% is the optimal rate for alleviating soil salinity in saline–alkali soils. This research demonstrated the potential of biochar to improve soil properties and promote crop growth and provided useful information on biochar application rates for ameliorating saline–alkali soils.

Green manure removal with reduced nitrogen improves saline-alkali soil organic carbon storage in a wheat-green manure cropping system

The incorporation of green manure into cropping systems is a potential strategy for sequestering soil carbon (C), especially in saline-alkali soil. Yet, there are still unknown about the substitution impacts of green manure on nitrogen (N) fertilizer in wheat-green manure multiple cropping system. Herein, a five-year field experiment was performed to determine the impact of three levels of N fertilizer inputs [i.e., N fertilizer reduced by 0 % (100N), 10 % (90 N), and 20 % (80 N)] with aboveground biomass of green manure removal (0GM) and return (100GM) on soil organic carbon (SOC) storage and its primary determinants. The results demonstrated that no significant interaction on SOC storage was detected between green manure and N fertilizer management. 80 N enhanced SOC storage in bulk soil by 7.4 and 13.2 % in 0–20 cm soil depth relative to 100 N and 90 N (p < 0.05). Regardless of N fertilizer levels, compared with 100GM, 0GM increased SOC storage in bulk soil by 14.2–34.6 % in 0–40 cm soil depth (p < 0.05). This was explained by an increase in soil macro-aggregates (>2 and 0.25–2 mm) proportion contributing to SOC physical protection. Meanwhile, the improvement of SOC storage under 0GM was due to the decrease of soil C- and N-acquisition enzyme activities, and microbial resource limitation. Alternatively, the variation partitioning analyses (VPA) results further suggested that C- and N-acquisition enzyme activities, as well as microbial resource limitation were the most important factors for SOC storage. The findings highlighted those biological factors played a dominant role in SOC accumulation compared to physical factors. The aboveground biomass of green manure removal with N fertilizer reduced by 20 % is a viable option to enhance SOC storage in a wheat-green manure multiple cropping system.

Insights into “wheat aroma”: Analysis of volatile components in wheat grains cultivated in saline–alkali soil

The wheat grains that are cultivated in saline–alkali soil exhibit a richer “wheat aroma” compared to their counterparts. This study characterized the composition and content of volatiles in five wheat kernel varieties, harvested from two fields with varying pH levels and total salt content in the soil. The wheat grown in soil with high pH and total salt content had significantly lower levels (p < 0.05) of ethyl 3-methylbutanoate and 1-octen-3-one and significantly higher levels (p < 0.05) of 1-butanol and 1-octen-3-ol. Among all factors, plant site contributed the highest F-value contribution rate (more than 77 %) for these four volatile compounds. Six e-nose sensors responsive to these four compounds exhibited consistent trends. Therefore, the lower of ethyl 3-methylbutanoate and 1-octen-3-one, the higher of 1-butanol and 1-octen-3-ol in wheat, grown on saline–alkali soil, served as characteristic markers for “wheat aroma”.

Exploring Candidate Genes and Regulatory Mechanisms for Salt–Alkali Tolerance in Cucumber

Soil salinization is one of the main problems faced by modern agricultural production, especially cucumber production. This study screened the salt–alkali-tolerant cultivar ‘D1909’ and the salt–alkali-sensitive cultivar ‘D1604’ from 32 different cucumber ecological types on the basis of morphological indicators and salt–alkali indices combined with relevant physiological and biochemical indices. By performing a transcriptome metabolome analysis, the key gene CsSRG1, which is responsive to salt–alkali stress in cucumber, was screened, and its function was verified. The role of CsSRG1 in reducing salt–alkali stress in cucumber was clarified, and the mechanism of salt–alkali tolerance in cucumber was preliminarily explored. This study provides germplasm resources for cucumber salt–alkali tolerance breeding and a theoretical basis for the effective use of saline alkali soil to achieve high quality and high yield in other crops.

Clonal integration reduced sexual reproduction of Leymus chinensis clones in heterogeneous environments regardless of stress/disturbance intensities

Clonal integration can benefit the performance and fitness of clonal plants in heterogeneous environments. Although trade-offs of sexual and asexual reproduction are key life-history strategies to assess the plant fitness and adaptive abilities, the effects of clonal integration on these is less studied. In this study, a pot experiment was conducted to test how reproductive strategies were affected by clonal integration of clonal plant species Leymus chinensis growing in heterogeneous saline-alkali soils. The results showed that clonal integration had no significant effect on total biomass of whole clone of L. chinensis. The total biomass of younger ramets was higher (+32.8%, P < 0.001) under integration condition, while at the cost of the older ramets (-21.7%, P < 0.001). Moreover, the ratio of ear biomass to clonal growth organs (CGO) biomass was slightly reduced by 31.7% (P = 0.09). For younger ramets, clonal integration enhances biomass allocation towards CGO, while reducing allocation towards ear growth, and the ratio of ear/CGO biomass was significantly reduced by 39.1% (P < 0.01). Results indicated that clonal integration reduced the ratio of ear to clonal growth organ biomass of whole clone, and more biomass was allocated to younger ramets for spatial expansion. Clonal integration influenced reproductive strategies of L. chinensis, while its impact is not depended on nutrient levels or defoliation interference. The study emphasized the significance of clonal integration in promoting the growth of offspring ramets and, consequently, enhancing their abilities to expand rapidly. It could enhance the spatial expansion of L. chinensis on a site scale and provide high potential for restoration of patchy degraded grassland where it is dominant.

Alleviation of cotton growth suppression caused by salinity through biochar is strongly linked to the microbial metabolic potential in saline-alkali soil

Biochar is a typical soil organic amendment; however, there is limited understanding of its impact on the metabolic characteristics of microorganisms in saline-alkaline soil microenvironment, as well as the advantages and disadvantages of plant-microorganism interactions. To elucidate the mechanisms underlying the impact of saline-alkali stress on cotton, a 6-month pot experiment was conducted, involving the sowing of cotton seedlings in saline-alkali soil. Three different biochar application levels were established: 0 % (C0), 1 % (C1), and 2 % (C2). Results indicated that biochar addition improved the biomass of cotton plants, especially under C2 treatment; the dry weight of cotton bolls were 8.15 times that of C0. Biochar application led to a rise in the accumulation of photosynthetic pigments by 8.30–51.89 % and carbohydrates by 7.4–10.7 times, respectively. Moreover, peroxidase (POD) activity, the content of glutathione (GSH), and ascorbic acid (ASA) were elevated by 23.97 %, 118.39 %, and 48.30 % under C2 treatment, respectively. Biochar caused a reduction in Na+ uptake by 8.21–39.47 %, relative electrical conductivity (REC) of plants, and improved K+/Na+ and Ca2+/Na+ ratio indicating that biochar alleviated salinity-caused growth reduction. Additionally, the application of biochar enhanced the absorption intensity of polysaccharide fingerprints in cotton leaves and roots. Two-factor co-occurrence analysis indicated that the key differential metabolites connected to several metabolic pathways were L-phenylalanine, piperidine, L-tryptophan, and allysine. Interestingly, biochar altered the metabolic characteristics of saline-alkali soil, especially related to the biosynthesis and metabolism of amino acids and purine metabolism. In conclusion, this study demonstrates that biochar may be advantageous in saline soil microenvironment; it has a favorable impact on how plants and soil microbial metabolism interact.

Straw interlayer improves sunflower root growth: Evidence from moisture and salt migration and the microbial community in saline-alkali soil

A straw interlayer added to soil can effectively reduce soil salinity effects on plant growth, however, the effects of soil moisture, salt and microbial community composition on plant growth under a straw interlayer are unclear. A rhizobox study was conducted to investigate the role of straw interlayer thickness on soil moisture, salt migration, microbial community composition, as well as root growth in sunflower. The study included four treatments: Control (no straw interlayer); S3 (straw interlayer of 3.0 cm); S5 (straw interlayer of 5.0 cm); S7 (straw interlayer of 7.0 cm). Straw interlayer treatments increased soil moisture by 8.2–11.0% after irrigation and decreased soil salt content after the bud stage in 0–40 cm soil. Total root length, total root surface area, average root diameter, total root volume and the number of root tips of sunflower plants were higher under straw interlayer treatments than in the control, and were the highest under the S5 treatment. This stimulated root growth was ascribed to the higher abundance of Chloroflexi and Verrucomicrobia bacteria in soil with a straw interlayer, which was increased by 55.7 and 54.7%, respectively, in the S5 treatment. Addition of a straw interlayer of 5 cm thickness is a practical and environmentally feasible approach for improving sunflower root growth in saline-alkali soil.

The effect of modifier and a water-soluble fertilizer on two forages grown in saline-alkaline soil.

Saline-alkali soil significantly impairs crop growth. This research employs the impacts of the modifier and water-soluble fertilizer, as well as their interaction, on the root systems of alfalfa and leymus chinensis in saline-alkali soil. The results exhibit that the hydrochar source modifier effectively enhances the root growth of both forage species. There are certain improvements in the root growth indicators of both crops at a dosage of 20 g/kg. Root enzyme activity and rhizosphere soil enzyme activity are enhanced in alfalfa, showing significant improvements in the first planting compared to the second planting. The application of water-soluble fertilizers also promotes root growth and root dehydrogenase activity. The root dehydrogenase activity of alfalfa and leymus chinensis are enhanced 62.18% and 10.15% in first planting than that of blank, respectively. Additionally, the two-factor variance analysis revealed a correlation between rhizosphere soil enzyme activity and changes in root traits. Higher rhizosphere soil enzyme activity is observed in conjunction with better root growth. The combined application of a modifier and water-soluble fertilizer has demonstrated a significant interaction effect on various aspects of the first planting of alfalfa and leymus chinensis. Moreover, the combined application of the modifier and water-soluble fertilizer has yielded superior results when compared to the individual application of either the modifier or the water-soluble fertilizer alone. This combined approach has proven effective in improving saline-alkali soil conditions and promoting crop growth in such challenging environments.

Vegetative cell wall protein OsGP1 regulates cell wall mediated soda saline-alkali stress in rice.

Plant growth and development are inhibited by the high levels of ions and pH due to soda saline-alkali soil, and the cell wall serves as a crucial barrier against external stresses in plant cells. Proteins in the cell wall play important roles in plant cell growth, morphogenesis, pathogen infection and environmental response. In the current study, the full-length coding sequence of the vegetative cell wall protein gene OsGP1 was characterized from Lj11 (Oryza sativa longjing11), it contained 660 bp nucleotides encoding 219 amino acids. Protein-protein interaction network analysis revealed possible interaction between CESA1, TUBB8, and OsJ_01535 proteins, which are related to plant growth and cell wall synthesis. OsGP1 was found to be localized in the cell membrane and cell wall. Furthermore, overexpression of OsGP1 leads to increase in plant height and fresh weight, showing enhanced resistance to saline-alkali stress. The ROS (reactive oxygen species) scavengers were regulated by OsGP1 protein, peroxidase and superoxide dismutase activities were significantly higher, while malondialdehyde was lower in the overexpression line under stress. These results suggest that OsGP1 improves saline-alkali stress tolerance of rice possibly through cell wall-mediated intracellular environmental homeostasis.