Oilseed Research Articles

Physicochemical Properties of Different Melon Seed Oils Dried at Different Temperatures

In this study, the effect of the drying process on the quality of oils obtained from different melon seeds was investigated. The seeds of two different melon varieties (Kırkağaç, Hasanbey) were separated and dried in the oven at different temperatures (30, 50 and 70 °C). Moisture, 1000 grain weight, width, length, thickness, oil yield, free fatty acidity, peroxide value, color values (L*, a*, b*), and total phenolic substance amounts and fatty acid composition were examined. The average of initial moisture values was 6.42% and 5.48%. The drying process was carried out until the moisture rate of melon seeds reached 1%. The average of most values of the cores varied from 4.55-5.16 mm, length 12.82-13.18 mm, thickness 1.21-1.38 mm and 1000 grain weights varied from 52.40-45.70 g. The amount of total phenolic substances of Kırkağaç melon seed oil was found in the range of 584-691 mg GAE /kg, and Hasanbey melon seed oil in the range 780-840 mgGAE / kg. The amount of total phenolic substance decreased with the applied heat treatment. The peroxide values of the oil obtained from Kırkağaç and Hasanbey beans in terms of meqO2 / kg varied between 6.9-8.4 and 7.2-9.1, respectively. The highest peroxide value was observed in oil obtained from beans dried at 70°C in both varieties. It has been observed that oleic and palmitic acids are also followed by linoleic acid compared to fatty acid composition compared to other fatty acids. According to the results obtained, it was determined that the most effective drying temperature was 50°C in terms of efficiency and quality.

Preparation and Application of Black Cumin Seed Oil Emulsion with Enhanced Stability for Antimicrobial Treatment of Cellulosic Fabric

Preparation and Application of Black Cumin Seed Oil Emulsion with Enhanced Stability for Antimicrobial Treatment of Cellulosic Fabric

Rapid single flax (Linum usitatissimum) seed phenotyping of oil and other quality traits using single kernel near infrared spectroscopy

AbstractThe growing interest in the rapid measurement of seed ingredients using single‐kernel NIR (SKNIR) spectroscopy as a nondestructive measurement technique allows fast analysis of sample seed variance that can have effects on breeding and end‐use processing. Flax (Linum usitatissimum), an oilseed crop grown in the Northwest United States and worldwide, is highly beneficial for human health, food, and fiber. Its health benefits include its high protein and omega‐3 fatty acids content. Therefore, seed composition profiles are an important aspect of breeding. The goals of this research were the development of single seed NIR calibration models for protein, oil, and weight of intact flax seeds. In this study, SKNIR spectroscopy was used on a diverse set of flax accessions comprising of 306 samples to create prediction models on a custom built SKNIR instrument. Spectra data and reference protein, oil, and weight were used to build partial least squares (PLS) models. Calibration models provided reasonable prediction of these traits and could be used for screening purposes. PLS statistics were oil (R2 = 0.82, SEP = 1.72), weight (R2 = 0.74, SEP = 0.71), and protein (R2 = 0.62, SEP = 0.96) for validation data sets comprising of one‐third of the total samples. In conclusion, prediction models showed that SKNIR spectroscopy could be a very beneficial nondestructive technique to determine oil and weight as well as rapid screening of protein in single flax seeds while not requiring extensive preparation as compared to traditional techniques.

Histological Study on the Possible Protective Effect of Pumpkin Seed Oil on Cyclophosphamide Induced Hemorrhagic Cystitis in Adult Male Albino Rat

Histological Study on the Possible Protective Effect of Pumpkin Seed Oil on Cyclophosphamide Induced Hemorrhagic Cystitis in Adult Male Albino Rat

Cold Hydraulic Extraction Optimization and Characterization of Balanites aegyptiaca and Ceiba pentandra Seed Oils

Non-edible vegetable oils have a huge potential for many industrial applications. Most of them are expressed by mechanical pressing, but data on the different operating conditions to achieve optimal extraction yields and high-quality oil are scarce. Hence, the objective of this study was to optimize key hydraulic extraction parameters at ambient temperature (25 °C) of <em>Balanites aegyptiaca</em> and <em>Ceiba pentandra</em> oils by increasing the oil recovery. Optimal holding time, applied pressure, compression speed, and press cage charge were determined for whole and crushed oleaginous material. The results indicate that the <em>B.aegyptiaca </em>kernels and <em>C.pentandra</em> seeds contained 2.7 and 5.7% moisture, as well as 46.3 and 26.2% fat, respectively. Under the optimal operating conditions, the oil recovery (wt.%. d.b.) from whole (crushed) material was 73.4 (69.8) <em>B.aegyptiaca </em>kernels and 38.2 (41.2) <em>C.pentandra</em> seeds.<strong> </strong>The extracted oils have a high content of unsaturated fatty acids including of about 74 wt.% for <em>B. aegyptiaca</em> and 69 wt.% for <em>C.pentandra</em>. They were particularly rich in linoleic ω-6 acid, 45.32 and 38.77 wt.%, respectively. The chemical characteristics of cold-extracted <em>B. aegyptiaca</em> and <em>C.pentandra</em> oils were 10 and 20 ppm phosphorus, 2.8 and 3.5 ppm iron, 0.0 ppm copper, 0.06 and 0.1% water-volatile matter, respectively. These valuable material properties make the <em>B.aegyptiaca</em> and <em>C.pentandra</em> oils an ecological and renewable resource for multipurpose applications.

Development and Characterization of Ethylcellulose Oleogels Based on Pumpkin Seed Oil and Rapeseed Oil.

In contrast to rapeseed oil, pumpkin seed oil has yet to be well investigated in terms of oleogelation, and, to the best of our knowledge, no study related to the use of ethylcellulose (EC) in the structuring of this oil has been identified in the current scientific literature. Therefore, the present study evaluated several oleogels formulated with EC as the oleogelator in different concentrations of 7% (OG7) and 9% (OG9), based on cold-pressed pumpkin seed oil (PO) and refined rapeseed oil (RO), as well as on mixtures of the two oils in different combinations: PO:RO (3:1) (PRO) and PO:RO (1:1) (RPO). Physicochemical properties such as visual appearance, gel formation time (GFT), oil-binding capacity (OBC), oxidative and thermal stability, and textural characteristics were analyzed. Analysis of variance (ANOVA) and Tukey's honestly significant difference (HSD) were used in the statistical analysis of the data, with a significance level of p < 0.05. EC proved to be an effective structuring agent of the mentioned edible oils; the type of oils and the concentration of oleogelator significantly influenced the characteristics of the obtained oleogels. The 9% EC oleogels exhibited a more rigid structure, with a higher OBC and a reduced GFT. Pumpkin seed oil led to more stable oleogels, while the mixture of pumpkin seed oil with rapeseed oil caused a significant reduction in their mechanical properties and decreased the OBC. After 14 days of storage, all oleogels demonstrated proper oxidative stability within the bounds set by international regulations for edible fats, regardless of the kind of oil and EC concentration. All of the oleogels showed a higher oxidative stability than the oils utilized in their formulation; however, those prepared with cold-pressed pumpkin seed oil indicated a lower level of lipid oxidation among all oleogels. The P-OG9 and PR-OG9 oleogels, which mainly included PO and contained 9% EC, demonstrated the optimum levels of quality in texture, GFT, OBC, and oxidative stability.

Effects of temperature and pressure on volatile compounds of black cumin seeds (Nigella sativa L.) oil extracted by supercritical carbon dioxide

AbstractThis study investigated the effects of temperature and pressure on the yield and volatile compound contents of black cumin seed oil (BCO) extracted from black cumin seeds using supercritical carbon dioxide (SCCO2). The solubility of BCO in SCCO2 increased 2.5‐fold as the pressure increased by 10 MPa. Major volatile compounds in BCO were identified using a static headspace gas chromatography–mass spectrometry (SH–GC–MS). Evidence indicated the main volatile components in SCCO2‐extracted BCO to be hexanal, p‐cymene, and thymoquinone. BCO extracted from China‐cultivated black cumin seed contained similar amounts of p‐cymene and thymoquinone. This black cumin seed is classified as the cymene/thymoquinone chemotype and is also found in Indian, Iranian, and Algerian black cumin seeds. Thymoquinone contents in BCO were more sensitive to the extraction temperature than hexanal and p‐cymene contents. The maximum oil yield of 36.28 ± 1.38 wt%, with approximately 2.0 mg of thymoquinone per milliliter of oil, was obtained at extraction temperatures of 50°C and pressures of 30 MPa. A SH–GC–MS could detect small molecules in black cumin seed oil such as hexanal. The results of this work reveal that temperature has more impact on the selectivity of volatile compounds than pressure in supercritical carbon dioxide extraction.

The role of pan‐roasting on changes in bioactive compounds, phenolic and fatty acid composition of chia (Salvia hispanica L.) seeds

SummaryIn this study, the effect of roasting on the oil quantities, bioactive properties, colour, fatty acids and polyphenols of seeds roasted in pan at roasting times was investigated. Total phenolic and flavonoid quantities of roasted seeds are assayed among 186.59 (control) and 429.29 mgGAE/100 g (20 min) to 810.00 (10 min) and 903.33 mg/100 g (5 min), respectively. The heat treatment increased the oil and total phenol quantities of the chia seeds compared to the control, depending on the increase in roasting time. In addition, the antioxidant effect of the seeds in the 5‐min roasting process was the same as in the control, but it showed differences with other roasting times. As the roasting time increased, a significant increase was observed in the L* values of the seeds and this reduced the brightness of the seeds. The 3,4‐dihydroxybenzoic acid and catechin quantities of the chia seeds roasted for 15 and 20 min were higher than the 3,4‐dihydroxybenzoic acid and catechin amounts of control and chia seeds roasted for 5 and 10 min. Linolenic acid was found to be the highest in chia oils, and its amount changed between 63.66 (20 min) and 66.88% (control).

Consumption of a Byproduct of Chia Seed Oil Extraction by Cold Pressing Ameliorates Cardiovascular Risks Factors in an Experimental Model of Metabolically Unhealthy Normal Weight.

The byproduct of Salvia hispanica (chia) seed oil extraction by cold pressing, also known as expeller, possesses a high nutritional value. It is rich in proteins, fibers, minerals, and has a residual oil content of 7-11%, which is rich in omega 3 linolenic acid (ALA). However, this byproduct has been historically undervalued. Thus, the aim of current work was to study the effects of consuming of a rich in chia expeller diet on a rabbit model of metabolically unhealthy normal weight to validate their use as a functional food. Rabbits were fed different diets for a period of 6 weeks: a standard diet (CD), a high-fat diet (HFD), a rich in expeller CD (Exp-CD) and a rich in expeller HFD (Exp-HFD). The Exp-HFD attenuated the rise in basal glucose, TyG index, triglycerides, cholesterol and non-HDL cholesterol induced by the HFD. Both rich in expeller diets reduced mean arterial blood pressure (MAP) and increase liver and fat ALA levels compared to their respective controls. Furthermore, the angiotensin converting enzyme (ACE) activity was lower in the lungs of animals fed on rich in expeller diets compared to their respective controls. In vitro studies showed that ALA inhibited ACE activity. The evaluation of vascular reactivity revealed that rich in expeller diets improved angiotensin II affinity and reduced contractile response to noradrenaline. In conclusion, the consumption of rich in expeller diets showed beneficial effects in preventing cardiovascular risk factors such as insulin resistance, dyslipidemia and MAP. Therefore, its use as functional ingredient holds significant promise.

Formulation and Characterization of Ethosomes for Transdermal Delivery of Prinsepia Utilis Rogle Seed Oil with Ameliorative Effects against UVB-Induced Skin Damage.

Prinsepia utilis seed oil (PUSO) is a natural medication obtained from Prinsepia utilis Rogle seed, which has been used for the treatment of skin diseases. The study aims to prepare ethosomes with high drug loading as a water-soluble transdermal vehicle to enhance the transdermal delivery of PUSO. PUSO-loaded ethosomes (PEs) were prepared using a cold method, and optimized by an orthogonal experimental design with entrapment efficiency (EE) as the dependent variable. The PEs prepared with the optimized formulation showed good stability, with a spherical shape under transmission electron microscopy (TEM), average particle size of 39.12 ± 0.85 nm, PDI of 0.270 ± 0.01, zeta potential of -11.3 ± 0.24 mV, and EE of 95.93 ± 0.43%. PEs significantly increased the skin deposition of PUSO compared to the PUSO suspension (P < 0.001). Moreover, the optimum formula showed significant ameliorative effects on ultraviolet B (UVB) irradiation-associated macroscopic and histopathological changes in mice skin. Therefore, PEs represent a promising therapeutic approach for the treatment of UVB-induced skin inflammation, with the potential for industrialization.

Updated Review on: Cucurbita Maxima (Pumpkin Seed) oil for acne relief patches

Acne being one of the dermatologic problems that affect people at different ages has multiple origins. The purpose of this review is to address whether or not pumpkin seed oil functions as an effective therapy for acne and could be used alongside acne patches. In the introduction, different types of acne are explained with some explanation of how such is developed. This is a crucial introduction to a detailed analysis of how Pumpkin seed oil may be useful in managing acne. The review focuses the diverse types of acne, ranging from non-inflammatory comedowns to inflammatory papules and nodules, highlighting the need for targeted and effective treatment strategies. Pumpkin seed oil, consist of essential fatty acids, antioxidants, and anti-inflammatory properties, emerges as a promising candidate for acne treatment. Review explore about acne patches as a novel and targeted delivery system form pumpkin seed oil. Different types of acne patches are discussed, each designed to address specific aspects of acne pathogenesis.

Thiourea improves yield and quality traits of Brassica napus L. by upregulating the antioxidant defense system under high temperature stress

High temperature stress influences plant growth, seed yield, and fatty acid contents by causing oxidative damage. This study investigated the potential of thiourea (TU) to mitigate oxidative stress and restoring seed oil content and quality in canola. The study thoroughly examined three main factors: (i) growth conditions—control and high temperature stress (35 °C); (ii) TU supplementation (1000 mg/L)—including variations like having no TU, water application at the seedling stage, TU application at seedling stage (BBCH Scale-39), water spray at anthesis stage, and TU application at anthesis stage (BBCH Scale-60); (iii) and two canola genotypes, 45S42 and Hiola-401, were studied separately. High temperature stress reduced growth and tissue water content, as plant height and relative water contents were decreased by 26 and 36% in 45S42 and 27 and 42% Hiola-401, respectively, resulting in a substantial decrease in seed yield per plant by 36 and 38% in 45S42 and Hiola-401. Seed oil content and quality parameters were also negatively affected by high temperature stress as seed oil content was reduced by 32 and 35% in 45S42 and Hiola-401. High-temperature stress increased the plant stress indicators like malondialdehyde, H2O2 content, and electrolyte leakage; these indicators were increased in both canola genotypes as compared to control. Interestingly, TU supplementation restored plant performance, enhancing height, relative water content, foliar chlorophyll (SPAD value), and seed yield per plant by 21, 15, 30, and 28% in 45S42; 19, 13, 26, and 21% in Hiola-401, respectively, under high temperature stress as compared to control. In addition, seed quality, seed oil content, linoleic acid, and linolenic acid were improved by 16, 14, and 22% in 45S42, and 16, 11, and 23% in Hiola-401, as compared to control. The most significant improvements in canola seed yield per plant were observed when TU was applied at the anthesis stage. Additionally, the research highlighted that canola genotype 45S42 responded better to TU applications and exhibited greater resilience against high temperature stress compared to genotype Hiola-401. This interesting study revealed that TU supplementation, particularly at the anthesis stage, improved high temperature stress tolerance, seed oil content, and fatty acid profile in two canola genotypes.

Assessment of Physicochemical Properties of Laundry Soaps Prepared From Mango and Avocado Seed Oils and Their Blends: Giving Attention to Fruit Left Overs

Assessment of Physicochemical Properties of Laundry Soaps Prepared From Mango and Avocado Seed Oils and Their Blends: Giving Attention to Fruit Left Overs

Combining Ability, Heritability, and Heterosis for Seed Weight and Oil Content Traits of Castor Bean (Ricinus communis L.)

Hybridization is an important evolutionary force, and heterosis describes the phenomenon where hybrids exhibit superior traits compared to their parents. This study aimed to evaluate the one-hundred-seed weight and fatty acid content in F1 generations, investigating the effects of different parental crosses using a 9 × 3 incomplete diallel design (NCII). One of the challenges faced in this study was the complexity of accurately determining the influence of both genetic and environmental factors on trait inheritance. A total of 36 F1 crosses were analyzed for general combining ability (GCA), specific combining ability (SCA), and heritability. The results showed that the level of each index in F1 is closely related to its parents. Significant differences in GCA and SCA were observed among parental traits in most crosses. The ratio of GCA to SCA ranged from 0 to 3, indicating the pivotal role of SCA over GCA in castor breeding efforts. High narrow-sense heritability was recorded in palmitic acid (30.98%), oleic acid (28.68%), and arachidonic acid (21.34%), suggesting that these traits are predominantly under the control of additive gene action, and hence these characters can be improved by selection. Additionally, heterosis exhibited diverse patterns across traits. Based on the evaluated combining ability, heritability, and heterosis, the inbred lines CSR181 and 20111149 were recommended for castor crossbreeding due to their potential to yield progeny with optimal oil-related traits. This research contributes valuable knowledge to the field of castor breeding, providing a foundation for developing superior castor cultivars.

Effects of environmental factors and storage periods on sesame seed quality and longevity

AbstractSesame is one of the world’s oldest oil seed crops grown mainly for its seeds. Lengthened storage time, inappropriate staking, back warded threshing method and poor storage facilities are major causes for postharvest and quality losses of sesame seed. Therefore, the objective was to review the effect of storage time and storage conditions on sesame seed quality and longevity. Seed quality and longevity are mostly governed by moisture content, temperature, humidity, storage period, pre-storage conditions, and pest infestations. Moisture content is a pre-requisite for long-term seed storage, and is the most important factor affecting seed longevity. Sesame seeds remain consistent and viable up to 12 months under appropriate storage facilities and conditions, otherwise it get lessened its viability. The crop should be harvested at the appropriate time and seeds should be stored at 6% or lower moisture content. In the tropics where temperature is as high as 33 °C and relative humidity of about 80%, seeds deteriorate rapidly. Varietal mixtures and harvest conditions affect longevity of seeds in storage. Pests such as, Mites, Indian mealy moth, Weevils, Flour beetles, are serious problems in stored sesame seeds. Under low seed moisture content and temperature, insects may not be a problem. Oil seeds require high-quality constructions to prevent leakages and to allow easy access to the bin for sampling and monitoring. Therefore, sesame seeds should be stored in well-constructed room/ware house to maintain seed viability and longevity.

Sustainable and Green Production of Biodiesel from Calotropis procera Seed Oil Using CuO Nanocatalyst

Sustainable and Green Production of Biodiesel from Calotropis procera Seed Oil Using CuO Nanocatalyst

Processing and Analysis of Sinapis Alba (Yellow Mustard) Seeds Oil Obtained by Cold Pressed Method

Yellow mustard oil is made from the seeds of the yellow mustard plant, scientifically known as Sinapis Alba. The seeds are cold-pressed to extract the oil, ensuring that the natural flavors and nutrients are preserved. This traditional method of extraction, known as cold pressed method, allows the oil to retain its natural properties and distinct flavor.

Composition of Amesiodendron chinense (Merr.) Hu Seed Oil and Assessment of Its Nrf2/ARE Induction Activity in AREc32 Cells

Article Composition of Amesiodendron chinense (Merr.) Hu Seed Oil and Assessment of Its Nrf2/ARE Induction Activity in AREc32 Cells Chuanchom Khuniad 1,2,*, Lutfun Nahar 3,*, Jason W. Birkett 4, Kenneth J. Ritchie 1 and Satyajit D. Sarker 1,* 1 Centre for Natural Products Discovery (CNPD), School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool L3 3AF, UK 2 Department of Thai Traditional Medicine, Faculty of Health and Sports Science, Thaksin University, Phatthalung 93210, Thailand 3 Laboratory of Growth Regulators, Palacký University and Institute of Experimental Botany, The Czech Academy of Sciences, Šlechtitelů 27, 78371 Olomouc, Czech Republic 4 Forensic Science Research Group, School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool L3 3AF, UK * Correspondence: C.Khuniad@2020.ljmu.ac.uk (C.K.); nahar@ueb.cas.cz (L.N.) and S.Sarker@ljmu.ac.uk (S.D.S.) Received: 7 April 2024; Revised: 26 April 2024; Accepted: 6 May 2024; Published: 14 May 2024 Abstract: Background: Amesiodendron chinense (Merr.) Hu (family: Sapindaceae) is a Thai medicinal plant. The seed oil of this species has been used by folk healers and local people in southern Thailand for the treatment of wounds, skin disorders and common hair problems. This study aimed at the GC-MS-based determination of the chemical composition of the seed oil of this plant, and evaluation of its Nrf2/ARE induction activity in AREc32 cells (modified human breast cancer cell line MCF-7) using the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] and luciferase reporter gene assays. Results: GC-MS analysis identified 9-(E)-octadecenoic acid (84.82%) as the main component of this seed oil. TLC-based qualitative DPPH (2,2-diphenyl-1-picrylhydrazyl) assay revealed the DPPH radical-scavenging activity of the seed oil and its chromatographic fractions. A low-level DPPH-scavenging activity was observed in the quantitative assay, but no IC50 value could be determined even with the highest tested concentration (10 mg/mL). Neither the oil nor its chromatographic fractions showed any significant Nrf2/ARE induction in AREc32 cells. The seed oil was noncytotoxic against the AREc32 cells. Conclusions: A. chinense seed oil and its fractions had a low level of free-radical scavenging property but no significant Nrf2/ARE induction activity in AREc32 cells. However, as the oil did not show any cytotoxicity at test concentrations in the MTT assay, this oil might potentially be safe to use in cosmetic formulations or as a vehicle for the dermal delivery of drug molecules.

Comparative transcriptomic analysis provides insights into the genetic networks regulating oil differential production in oil crops

BackgroundPlants differ more than threefold in seed oil contents (SOCs). Soybean (Glycine max), cotton (Gossypium hirsutum), rapeseed (Brassica napus), and sesame (Sesamum indicum) are four important oil crops with markedly different SOCs and fatty acid compositions.ResultsCompared to grain crops like maize and rice, expanded acyl-lipid metabolism genes and relatively higher expression levels of genes involved in seed oil synthesis (SOS) in the oil crops contributed to the oil accumulation in seeds. Here, we conducted comparative transcriptomics on oil crops with two different SOC materials. In common, DIHYDROLIPOAMIDE DEHYDROGENASE, STEAROYL-ACYL CARRIER PROTEIN DESATURASE, PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASE, and oil-body protein genes were both differentially expressed between the high- and low-oil materials of each crop. By comparing functional components of SOS networks, we found that the strong correlations between genes in “glycolysis/gluconeogenesis” and “fatty acid synthesis” were conserved in both grain and oil crops, with PYRUVATE KINASE being the common factor affecting starch and lipid accumulation. Network alignment also found a conserved clique among oil crops affecting seed oil accumulation, which has been validated in Arabidopsis. Differently, secondary and protein metabolism affected oil synthesis to different degrees in different crops, and high SOC was due to less competition of the same precursors. The comparison of Arabidopsis mutants and wild type showed that CINNAMYL ALCOHOL DEHYDROGENASE 9, the conserved regulator we identified, was a factor resulting in different relative contents of lignins to oil in seeds. The interconnection of lipids and proteins was common but in different ways among crops, which partly led to differential oil production.ConclusionsThis study goes beyond the observations made in studies of individual species to provide new insights into which genes and networks may be fundamental to seed oil accumulation from a multispecies perspective.

Antioxidant and Hepatoprotective Effect of Rosa davurica Pall Seed Oil on CCl4-Induced Acute Liver Injury in Mice.

The liver, being the most metabolically active organ, is highly vulnerable to damage caused by oxidative stress. Rosa davurica Pall. seed oil (RDPO), a novel vegetable oil, and its bioactive components have been extensively researched in the field of antioxidants. In this research, the antioxidant properties and hepatoprotection by RDPO were evaluated. A series of antioxidant evaluation systems and a CCl4-induced acute liver injury model in mice were used to investigate the antioxidant activity and hepatoprotective efficacy of RDPO. The results showed that the extraction rate of RDPO was 11.12% using the optimal extraction process. Three major unsaturated fatty acids of the oil were α-linolenic acid (11.89 ± 0.017%), linoleic acid (18.52 ± 0.072%), and oleic acid (11.54 ± 0.425%). Furthermore, its antioxidant small-molecule compounds were β-sitosterol (1.429 ± 0.002 μg/g), α-tocopherol (1.273 ± 0.079 μg/g), β-carotene (0.012 ± 0.001 μg/g), lycopene (0.108 ± 0.002 μg/g), squalene (178.950 ± 0.794 μg/g), total polyphenols (1.114 ± 0.032 μg GAE/mg), and total flavonoids (0.504 ± 0.009 mg RU/g), respectively. In vitro, RDPO significantly inhibited the production of ABTS+•, DPPH•, O2•-, and hydroxyl radicals, as well as Fe3+. In vivo, RDPO significantly reversed the activity of total superoxide-dismutase, catalase, L-glutathione, and the level of malondialdehyde (MDA) in liver tissue. It also obviously inhibited the activity of aspartate transaminase (AST) and the level of MDA in the serum. Therefore, RDPO has demonstrated excellent antioxidant activity and a potential liver protective effect. This effect may be ascribed to its capacity for decreasing AST activity, inhibiting lipid peroxidation, and boosting endogenous antioxidant enzyme activity. Therefore, RDPO has significant application value in the biopharmaceutical industry and as a dietary supplement.