The green microalga Monoraphidium sp. has potential for biodiesel production since it grows fast and can accumulate high levels of lipids. However, there is a lack of information on the potential use in human nutrition. In this work Monoraphidium sp. was characterized in terms of macronutrients with a special focus on the fatty acid profile of the lipid fraction and on the amino acid profile of the proteins. Furthermore, aiming at nutrient valorization, various methods for lipid extraction that could be used in the production of high quality and safe foods were investigated. To this end, the focus was on cell disruption methods in order to optimize oil recovery. The Monoraphidium sp. biomass had a high protein concentration (44.5 %) and a relatively low lipid concentration (12.5 %) but was rich in ω-3 fatty acids demonstrating its high nutritive value. Regarding cell disruption, ultrasonication and high-speed homogenization were insufficient to disrupt cells under the conditions examined. On the other hand, the effectiveness of the ball milling was regulated via processing time and water-to-biomass ratio and complete cell disruption could be achieved by this method. For lipid extraction, solvent extraction using a mixture of food-grade hexane and ethanol resulted in an oil recovery of 70.4 %, whereas using ethanol alone as an environmentally friendly solvent resulted in an oil recovery equal to 54 %. Supercritical CO2 extraction resulted in a lower oil recovery (25 %), whereas ethanol addition, as a cosolvent to CO2, significantly increased the oil recovery (60 %).