ABSTRACT X-ray imaging plays a crucial role in medical diagnosis, particularly for patients presenting with severe symptoms who require radiographic examination for further evaluation. While uncommon, there are instances where nurses may be exposed to radiation, such as when performing certain procedures on patients. The study employs an explanatory sequential mixed-method research aims to analyze the level of awareness of nurses on radiation exposure, identify predictive factors, and develop guidelines for promoting radiation protection awareness among professional nurses in Royong Hospital. Quantitative research involved a simple random sampling of 120 professionals who completed a 23-item questionnaire, with results analyzed using percentage, mean, standard deviation, and multiple regression analysis. Qualitative research included a purposive selection of 10 professional nurses for in-depth interviews, with data analyzed thematically. Results showed a high level of awareness, with predictive factors including gender (95.8% female, P < 0.05), age (highest awareness in 31–40 years range, 42.50%, P < 0.01), work experience (highest awareness in 11–15 years range, 27.50%, P < 0.01), and training on radiation protection (27.50%, P < 0.01). Guidelines for promoting awareness through radiation protection training and online hospital resources. It concludes that prioritizing radiation safety education is key to enhancing awareness among professional nurses in hospitals.