Safe Status Research Articles

In-package fumigation of blueberries using ethyl formate: Effects on spotted-wing drosophila (Drosophila suzukii Matsumura) mortality and fruit quality

Ethyl formate (EF) is a food-flavoring agent with a generally regarded as safe (GRAS) status. It is a naturally-occurring insecticidal volatile compound promising as an alternative to synthetic insect pest fumigants that are toxic and/or undesirable to the environment. In this study, an EF precursor (EFP) was investigated as an activated EF release system for in-package fumigation of spotted-wing drosophila (SWD) in blueberries. EF concentration, exposure time, and fruit loading factor had significant (p < 0.05) effects on the mortality of SWD adults. At 22 ± 1 °C, complete control (100 % mortality) of SWD adults was observed after exposing the infested fruits to a package headspace containing 6 mg/L of EF for 4 h at 3 and 6 % (w/v) fruit loading factors, while 4 mg/L EF dosage for 2 h resulted in a similar 100 % mortality at 30 % (w/v) fruit load factor. SWD eggs, larvae, and pupae in blueberries were more tolerant toward EF vapor than the adults, in which 16 mg/L of EF for 24 h was effective for complete control of these stages of the insect. There were no significant (p > 0.05) differences observed in blueberries quality parameters after the EF fumigation treatments. Results from this study suggested that in-package EF fumigation via the EFP system has a potential as a postharvest treatment for SWD control in blueberries. This active packaging approach can be beneficial for streamlining the existing fumigation approach, thereby increasing the available shelf-life of fresh produce.

Antagonistic, Anti-oxidant, Anti-inflammatory and Anti-diabetic Probiotic Potential of Lactobacillus agilis Isolated From the Rhizosphere of the Medicinal Plants.

Potential probiotic bacteria can be used as a biotherapeutic agent and a sustainable alternative to antibiotics, as an anti-oxidative, anti-inflammatory, and anti-diabetic agent without causing any serious side effects. Mostly human-friendly Lactic acid bacteria (LAB) have been isolated from the animal-human origin to be used as biotherapeutic agents or to produce useful metabolites (nutraceutical). However, less information is known about the role of medicinal plants associated LAB as biotherapeutic agents. The isolation of 115 human-friendly Lactobacillus strains was done from the rhizosphere of the medicinal plants Ocimum tenuiflorum, Azadirachta indica, Ficus carica. The obtained bacteria were then tested for their safe status before being using it for a beneficial purpose. Out of 115 strains, 29 (25%) were negative for blood hemolytic activities. Among these 29 isolates, three isolates did not show a breakdown of gelatin and were recognized as safe. Antibiotic resistance assay showed resistance of two of them against antibiotics discs of Streptomycin (10µg), Ciprofloxacin (20µg), Vancomycin (30µg), Metronidazole (10µg), Ampicillin (5µg), Chloramphenicol (30µg), Kanamycin (30µg), Erythromycin (15µg), Penicillin (10µg) and Tetracycline (30µg). The bacterial isolate (T-2) was found safe that was identified as Lactobacillus agilis by sequence analysis of 16s rRNA gene and processed in vitro as an anti-bacterial, anti-oxidant, anti-diabetic, and anti-inflammatory agent. Free radical scavenging activities and inhibition of α-amylase activities for Lactobacillus agilis were found relative to standard drug values as 68% and 73% and 51.3% and 65.3%, respectively. The in-vitro anti-inflammatory assay showed 61.6% (Lactobacillus agilis) while showed 69% (aspirin) activity for denaturation albumin protein. The results suggested that Lactobacillus agilis can be used as a potential probiotic strain as well as can be used to produce nutraceuticals.

“Digital Gold” and geopolitics

There is a growing empirical literature on Bitcoin and gold safe haven properties with respect to financial risks and macroeconomic news but very scarce literature regarding geopolitical risks. This paper provides a fresh insight into the Bitcoin safe haven status, in comparison to gold. We, first, propose a geopolitical risk composite indicator based on various sources of geopolitical risks. A Principal Component Analysis is conducted to group the information on these indicators. Second, a dynamic Markov-switching copula model (which accommodates a dynamic link between the developed geopolitical risk index and Bitcoin and gold price dynamics within low and high risk regimes) is used. We show that both Bitcoin and gold respond positively to the composite geopolitical risk indicator when risk is high. This underscores that both Bitcoin and gold have the ability to act as safe havens for assets whose valuations plummet during times of violent geopolitical conflicts. But such properties seem to be conditional upon different categories of geopolitical risks.

In situ DESI-MSI lipidomic profiles of mucosal margin of oral squamous cell carcinoma.

BackgroundAlthough there is consensus that the optimal safe margin is ≥ 5mm, obtaining clear margins (≥5 mm) intraoperatively seems to be the major challenge. We applied a molecular diagnostic method at the lipidomic level to determine the safe surgical resection margin of OSCC by desorption electrospray ionisation mass spectrometry imaging (DESI-MSI).MethodsBy overlaying mass spectrometry images with hematoxylin-eosin staining (H&E) from 18 recruited OSCC participants, the mass spectra of all pixels across the diagnosed tumour and continuous mucosal margin regions were extracted to serve as the training and validation datasets. A Lasso regression model was used to evaluate the test performance.FindingsBy leave-one-out validation, the Lasso model achieved 88.6% accuracy in distinguishing between tumour and normal regions. To determine the safe surgical resection distance and margin status of OSCC, a set of 14 lipid ions that gradually decreased from tumour to normal tissue was assigned higher weight coefficients in the Lasso model. The safe surgical resection distance of OSCC was measured using the developed 14 lipid ion molecular diagnostic model for clinical reference. The overall accuracy of predicting tumours, positive margins, and negative margins was 92.6%.InterpretationThe spatial segmentation results based on our diagnostic model not only clearly delineated the tumour and normal tissue, but also distinguished the different status of surgical margins. Meanwhile, the safe surgical resection margin of OSCC on frozen sections can also be accurately measured using the developed diagnostic model.FundingThis study was supported by Nanjing Municipal Key Medical Laboratory Constructional Project Funding (since 2016) and the Centre of Nanjing Clinical Medicine Tumour (since 2014).

Safety Evaluation of Bifidobacterium lactis BL-99 and Lacticaseibacillus paracasei K56 and ET-22 in vitro and in vivo.

Probiotics have been reported to play a major role in maintaining the balance of microbiota in host. Consumption of food with probiotics has increased with consumer concerns regarding healthy diets and wellness. Correspondingly, safety evaluation of probiotics for human consumption has become increasingly important in food industry. Herein, we aimed to test the safety of Bifidobacterium lactis BL-99 and Lacticaseibacillus paracasei K56 and ET-22 strains in vitro and in vivo. In results, these strains were found to be negative for mucin degradation and platelet aggregation test. Additionally, the three strains were susceptible to eight antibiotics. In accordance with bacterial reversion mutation (Ames) assay, the tested strains had no genetic mutagenicity. Finally, it was confirmed that there were no dose-dependent mortality and toxicity throughout multidose oral toxicity tests in rats. Our findings demonstrated that B. lactis BL-99 and L. paracasei K56 and ET-22 can achieve the generally recognized as safe (GRAS) status as probiotics in the future.

Production of proteins and commodity chemicals using engineered Bacillus subtilis platform strain.

Currently, increasing demand of biochemicals produced from renewable resources has motivated researchers to seek microbial production strategies instead of traditional chemical methods. As a microbial platform, Bacillus subtilis possesses many advantages including the generally recognized safe status, clear metabolic networks, short growth cycle, mature genetic editing methods and efficient protein secretion systems. Engineered B. subtilis strains are being increasingly used in laboratory research and in industry for the production of valuable proteins and other chemicals. In this review, we first describe the recent advances of bioinformatics strategies during the research and applications of B. subtilis. Secondly, the applications of B. subtilis in enzymes and recombinant proteins production are summarized. Further, the recent progress in employing metabolic engineering and synthetic biology strategies in B. subtilis platform strain to produce commodity chemicals is systematically introduced and compared. Finally, the major limitations for the further development of B. subtilis platform strain and possible future directions for its research are also discussed.

FEMA GRAS assessment of natural flavor complexes: Origanum oil, thyme oil and related phenol derivative-containing flavoring ingredients

In 2015, the Expert Panel of the Flavor and Extract Manufacturers Association (FEMA) initiated a re-evaluation of the safety of over 250 natural flavor complexes (NFCs) used as flavor ingredients, mostly consisting of a variety of essential oils and botanical extracts. This publication, seventh in the series, re-evaluates NFCs with constituent profiles dominated by phenolic derivatives including carvacrol, thymol and related compounds using a constituent-based procedure first published in 2005 and updated in 2018. The procedure is based on the chemical characterization of each NFC as intended for commerce and the estimated intake of the constituent congeneric groups. The procedure applies the threshold of toxicological concern (TTC) concept and evaluates relevant data on absorption, metabolism, genotoxic potential and toxicology of the constituent congeneric groups and the NFC under evaluation. Herein, the FEMA Expert Panel affirmed the generally recognized as safe (GRAS) status of seven phenolic derivative-based NFCs, Origanum Oil (Extractive) (FEMA 2828), Savory Summer Oil (FEMA 3013), Savory Summer Oleoresin (FEMA 3014), Savory Winter Oil (FEMA 3016), Savory Winter Oleoresin (FEMA 3017), Thyme Oil (FEMA 3064) and Thyme White Oil (FEMA 3065) under their conditions of intended use as flavor ingredients.

Prediction of the thermal safety of nitrocellulose package in large scale

In order to assess the risk of nitrocellulose (NC) package atuo-ignition in warehouse, the three kinetic parameters of thermal decomposition of NC were calculated based on DSC curves using commercial software AKTS-kinetic module. On this basis, effects of ambient temperature (Ta), initial temperature of the package (T0) and cylinder diameter of package on time to ignition (τ) were studied using AKTS-thermal safety module. Results showed that as Ta and/or T0 increases the τ decreases exponentially, but the τ at different package dimensions is related to the Ta. More importantly, for a certain Ta, the τ doesn’t always change monotonously with the dimension, there is a dangerous dimension which are easier to ignite. It indicates that big risks exist when trying to predict the safe status of a certain NC package by extrapolation.

The Safe Operation Status and Countermeasures of Transmission and Distribution Lines in Grid Planning and Design are Studied

The Safe Operation Status and Countermeasures of Transmission and Distribution Lines in Grid Planning and Design are Studied

Extraction, Functional Properties and Food Applications of Rapeseed Meal Protein Isolates

As a by-product of rapeseed oil extraction, rapeseed meal is rich in protein and balanced in amino acid composition, which is a high-quality plant protein resource, however, it is commonly used for animal feed resulted in a lower economic benefit. Rapeseed protein extracted from rapeseed meal has been documented in excellent functional properties including emulsifying properties, gelling ability and so on. Its biological activities such as antihypertensive activity and antioxidant properties are also reported. At present, rapeseed protein has generally recognized as safe status and approved for food use in the European Union and FDA. In the current article, the different methods to extract rapeseed protein from rapeseed meal, the functional properties and the application statue in food industry are reviewed to provide theoretical basis and technical support for the further development and rational application of rapeseed meal protein.

The occurrence, growth, and biocontrol of Listeria monocytogenes in fresh and surface-ripened soft and semisoft cheeses.

Listeria monocytogenes continues to pose a food safety risk in ready-to-eat foods, including fresh and soft/semisoft cheeses. Despite L. monocytogenes being detected regularly along the cheese production continuum, variations in cheese style and intrinsic/extrinsic factors throughout the production process (e.g., pH, water activity, and temperature) affect the potential for L. monocytogenes survival and growth. As novel preservation strategies against the growth of L. monocytogenes in susceptible cheeses, researchers have investigated the use of various biocontrol strategies, including bacteriocins and bacteriocin-producing cultures, bacteriophages, and competition with native microbiota. Bacteriocins produced by lactic acid bacteria (LAB) are of particular interest to the dairy industry since they are often effective against Gram-positive organisms such as L. monocytogenes, and because many LAB are granted Generally Regarded as Safe (GRAS) status by global food safety authorities. Similarly, bacteriophages are also considered a safe form of biocontrol since they have high specificity for their target bacterium. Both bacteriocins and bacteriophages have shown success in reducing L. monocytogenes populations in cheeses in the short term, but regrowth of surviving cells can commonly occur in the finished cheeses. Competition with native microbiota, not mediated by bacteriocin production, has also shown potential to inhibit the growth of L. monocytogenes in cheeses, but the mechanisms are still unclear. Here, we have reviewed the current knowledge on the growth of L. monocytogenes in fresh and surface-ripened soft and semisoft cheeses, as well as the various methods used for biocontrol of this common foodborne pathogen.

Essential Oils and Their Application in Food Safety

Food industries are facing a great challenge due to contamination of food products with different microbes such as bacteria, fungi, viruses, parasites, etc. These microbes deteriorate food items by producing different toxins during pre- and postharvest processing. Mycotoxins are one of the most potent and well-studied toxic food contaminants of fungal origin, causing a severe health hazard to humans. The application of synthetic chemicals as food preservatives poses a real scourge in the present scenario due to their bio-incompatibility, non-biodegradability, and environmental non-sustainability. Therefore, plant-based antimicrobials, including essential oils, have developed cumulative interest as a potential alternative to synthetic preservatives because of their ecofriendly nature and generally recognized as safe status. However, the practical utilization of essential oils as an efficient antimicrobial in the food industry is challenging due to their volatile nature, less solubility, and high instability. The recent application of different delivery strategies viz. nanoencapsulation, active packaging, and polymer-based coating effectively addressed these challenges and improved the bioefficacy and controlled release of essential oils. This article provides an overview of essential oils for the preservation of stored foods against bacteria, fungi, and mycotoxins, along with the specialized mechanism of action and technological advancement by using different delivery systems for their effective application in food and agricultural industries smart green preservative.

Phase Behavior of Non‐Ionic Surfactant‐Medium Chain Triglyceride‐Water Microemulsion Systems

AbstractMicroemulsion systems have garnered tremendous interest in the pharmaceutical sector for a variety of drug delivery applications. Non‐ionic surfactants are often the preferred surfactant class given their uncharged nature, enhanced oral safety profile, and generally regarded as safe status as compared to other surfactant classes (Myers, Surfactant science and technology, 2005, p. 29), (Malmsten, Handbook of microemulsion science and technology, 1999, p. 755), (Grove &amp; Mullertz, Chapter 5‐liquid self‐microemulsifying drug delivery systems, 2007), (Liu et al., Water‐insoluble drug formulation, 2008), (Hauss, Advanced Drug Delivery Reviews, 2007, 59, pp. 667–676), (Balazs, Solubility, delivery and ADME problems of drugs and drug‐candidates, 2011, p. 68). In this work, the phase behavior and microemulsion formation potential of four commonly used non‐ionic surfactants, PEG‐40 hydrogenated castor oil, Poloxamer 188, Polysorbate 80, and d‐α‐tocopherol polyethylene glycol succinate were studied via ternary phase diagram (TPD) mapping using a medium chain triglyceride, Miglyol 812. Results indicated notable differences in phase behavior despite similarities in hydrophilic–lipophilic balance value (13–15). All surfactants produced Winsor Type I, oil‐in‐water microemulsions at water concentrations above 40% wt/wt. Winsor Type II water‐in‐oil microemulsions were difficult to obtain even at high oil concentrations of ≥70% wt/wt. Winsor III microemulsions, though rare, were generally obtained in the middle regions of the TPD between 10% and 30% wt/wt water while Winsor IV microemulsions dominated at high surfactant concentrations of ≥45% wt/wt. Opaque emulsion areas were particularly notable in wax state surfactants. Polysorbate 80 and PEG‐40 hydrogenated castor oil demonstrated a high degree of synergism as well as the largest oil‐in‐water (o/w) and water‐in‐oil (w/o) microemulsion formation potential rendering them suitable for a number of enteral and parenteral applications.

278 Effect of Feeding CARS on Digestibility in Finishing Cattle Diets

Abstract An experiment was conducted to evaluate the digestibility of a novel liquid feed, Condensed Algal Residue Solubles (Veramaris, Blair, NE) in finishing cattle diets. Mass production of algae to harvest omega-3 fatty acids results in byproduct production of CARS (25.4% DM, 19.3% CP, 8.3% Fat, 9.96% Na on DM basis), made up of the de-oiled algae cells and residual fermentation substrates. The CARS product has generally recognized as safe (GRAS) status. Six ruminally and duodenally cannulated crossbred steers were assigned randomly to 1 of 3 treatments over 3 collection periods, for a 3 x 3 replicated Latin Square design. Treatments differed by increasing inclusion of CARS (0, 2.5, and 5% of diet DM) fed with CARS replacing steam flaked corn (72, 69.5, and 67% of diet DM as CARS inclusion increased). All diets contained 15% dry distillers grains, 8% alfalfa haylage, and 5% supplement. Cattle were dosed with 10 g of titanium dioxide per day. Duodenal and fecal samples were collected four times per day across four days and composited by period for each animal. Fecal samples were analyzed for titanium dioxide concentration to determine fecal output and diet digestibility. Data were analyzed with CARS inclusion and period as fixed effects and animal as a random effect. Orthogonal contrasts were used to test linear and quadratic effects of CARS inclusion. There were no significant differences for DM intake and OM intake between the different CARS inclusions (P ≥ 0.17), averaging 7.76 kg DM/d and 6.94 kg OM/d. Total tract DM digestibility was not affected by treatment (P ≥ 0.71) and averaged 73.0%. Total tract OM digestibility was also not different between treatments (P ≥ 0.93) and averaged 71.1%. Replacing steam flaked corn with CARS up to 5% of diet DM in finishing cattle diets did not affect diet digestibility.

Process parameters optimization for amylase production through rice bran bioconversion by Aspergillus niger

Enzymes are produced by a wide range of living things including bacteria, fungi, plants and animals. Fungal enzymes are preferred over other microbial sources owing to their widely accepted Generally Regarded As Safe (GRAS) status. Amylase is one of the most widely used enzymes in the biotechnology industry with a global market of over $4 bn as at 2015. However, the synthetic media used for the production of this enzyme is becoming costlier and has become a subject of concern for many food-processing industries. Hence, manufacturers are now in search for alternative approaches to cut down the cost of production. In addition, the Raw Materials Research and Development Council, Nigeria (rmrdc.gov.ng) indicated that the country spends about $200 bn annually on importation of industrial enzymes. Therefore, this study was designed with the aim of utilizing agro-industrial waste (rice bran) as the sole carbon source for the production of amylase in a Solid-State Fermentation (SSF) culture by optimizing the major process parameters. A 19-run experiment generated by Design Expert™ software was carried out to identify the optima for pH, temperature and substrate concentration that would yield the highest amount of amylase. The results of the current study showed that significant interaction effect exists between temperature and pH. However, no interaction was observed with substrate concentration and pH depicting the sensitivity of the enzyme to varying pH conditions. Similarly, a statistically significant 2FI model (p < 0.0022) that could be used to predict the yield of amylase was identified. Conclusively, the findings of this study suggests that the feasibility of using rice bran as an alternative carbon source in the production of amylase calls for its wider utilization in the food processing industries, thus, alleviating the cost concern.

Moral courage, moral sensitivity and safe nursing care in nurses caring of patients with COVID-19.

AimEvaluation the moral courage, moral sensitivity and safe nursing care in nurses caring of infected patients by the COVID‐19.DesignThis study employed cross‐sectional research.Methods520 nurses caring for COVID‐19 patients in 5 hospitals were selected via convenience sampling. They completed questionnaires online. Data were analysed by SPSS software version 22.ResultsFindings showed that moral courage has a strong and direct correlation with moral sensitivity (p < .001, r = 0–.70) and safe nursing care (p < .001, r = 0–.74). Variables of moral sensitivity, safe nursing care, work experience, age and employment status can predict 64.76% of the variance in moral courage in these nurses. Nursing care of patients with COVID‐19 have reported high moral courage in recent months, and in spite of the numerous tensions and stresses in terms of caring these patients during this relative long period, they are still diligent in providing safe and high sensitive care to these patients.

Identification of Traffic and Roadway Variables Affecting Safe Motorcycling Along Urban Roads

&#x0D; &#x0D; &#x0D; In Malaysia, motorcycles represented 45% of all registered vehicles. Alarmingly, motorcyclists comprise 65% of total road deaths. One major reason being that these vulnerable road users get entangled with other mixed vehicles. It is appreciated that roadway design guidelines are based on the characteristics of drivers/automobiles and not riders/motorcycles. Thus, motorcyclists are expected to occupy the dynamically changing space that is available along the roadway. An effective engineering measure to tackle motorcycle safety problems in mixed traffic conditions would be to segregate the motorcycles exclusively. However, this measure is not appropriate for urban roads. One practical approach to address motorcycling safety along urban roads is to identify the traffic and road environment variables that affects safe motorcycling. This list of variables can be used to check the safe motorcycling status along segments of urban roads. The variables relating to bicyclist's perception on the roadway segments were adopted for the variables affecting motorcycling safety along urban roads. To corroborate the adopted variables factors with the actual perception of motorcyclist, the questionnaires related to the variables affecting safe motorcycling were responded by 137 motorcyclists. The variables were identified to be pavement condition, on-street parking, traffic volume, divided/undivided roadway, left-side kerb clearance, lane width, and travelling speed. To further understand these seven variables from the aspect of motorcyclist perceptions, 14 short clips were presented to 483 motorcyclists who rated each clip based on their perception of safe motorcycling. Results found that the odds of feeling unsafe riding on bad pavement is 61.5 times greater than the odds of riding on good pavement. The odds of unsafe motorcycling along roads with on-street parking is 43.2 times higher than without on- street parking. Overall, it infers that the maintenance of road pavement conditions must be of high priority to the local authorities followed by issues of on-street parking along urban links.&#x0D; &#x0D; &#x0D;

Multiple Amino Acids Inhibit Postharvest Senescence of Broccoli

The function of free amino acids in protein synthesis, as a source of energy and unique roles in catabolism have been well studied in plant development but their function in postharvest fruit and vegetables has received little attention. This study evaluated 11 amino acids—arginine, alanine, aspartic acid, glutamic acid, glycine, ornithine, phenylalanine, serine, tyrosine, tryptophan and valine—on the development of senescence of broccoli. Broccoli florets were dipped in 5 mM solution of amino acids, then stored at 10 °C in air containing 0.1 µL L−1 ethylene. Senescence was assessed by green life, ethylene production, respiration rate and ion leakage. Green life was increased by all the amino acids except valine. Similarly, ethylene production and ion leakage were decreased by all the amino acids except valine, while respiration rate was reduced by all amino acids. It is speculated that the early reduction in ethylene production could be the mechanism by which the amino acids delayed senescence. The beneficial effect of naturally occurring amino acids in inhibiting senescence has potential commercial relevance, as the amino acids have Generally Recognised As Safe (GRAS) status which should assist gain regulatory approval, and gain acceptance by consumers wary of synthetic chemicals on foods.

POTENTIAL ANIMAL FEED RESOURCES BASED ON FOOD CROP WASTE SUPPORTS DEVELOPMENT OF BEEF CATTLE IN WEST SUMATERA, INDONESIA

The increase in the need for animal-based food consumption creates opportunities in the development of cattle farming in the future. But the problems in the field are a decrease in livestock productivity such as a decrease in livestock population and a decrease in livestock body weight, this is due to the lack of availability of sustainable animal feed. The solution offered is to use food crop waste as an alternative feed for livestock, so it is necessary to analyze the carrying capacity of food crop waste in an area. The purpose of this research is to analyze the potential resources of beef cattle feed based on food plant waste in West Sumatra Province and to analyze the nutritional content of food plant waste. The research was conducted in West Sumatra Province in January 2021. The method used was literature study and secondary data analysis. The data required consists of: (1) food crop production data, (2) beef cattle population data in West Sumatra Province. Data were analyzed by analyzing the carrying capacity of food plant waste. The results showed the availability of feed in West Sumatra Province 728,195.32 tonnes BKC / year, while the current total feed requirement is only 410,159.32 tonnes BKC / year. This shows the large potential for feed that has not been utilized, and this means that there is a large opportunity for Prov. West Sumatra in the future development of ruminants. The carrying capacity index of food plant waste (IDD) is 2.37, which means that it has a safe status where food plant waste is able to help support the needs of animal feed in West Sumatra Province. To increase the nutritional value of food plant waste, it can be overcome by giving a touch of waste processing technology innovations such as fermentation, silage, or ammonia. The results of this study are important as recommendations for the government regarding the sources of information on areas that have the potential to develop livestock business, which can be seen from the status of the area’s carrying capacity index or the potential availability of abundant animal feed.

Effects of hot water and calcium lactate treatments on fresh-cut quality of papaya

Purpose: Papaya is one of the most common fruits which has been produced in southern part of Iran for fresh consumption. Demand for fresh-cut products has increased due to the changes in consumer attitudes. However, fresh-cut papaya is prone to softening during storage. This study carried out to determine effects of calcium lactate and hot water treatments (HWT) for maintaining fresh-cut papaya quality. Research method: After preparing fresh cut cubes of papayas, the pieces were dipped in calcium lactate (CaL) or combined with HWT. Physico-chemical parameters including calcium concentration (Ca), firmness and soluble solids concentration, catalase and peroxidaseactivities, pectin methyl esterase (PME) and polygalacturonase activities (PG),total phenolic content and radical scavenging activities, and microbial growth were measured during 9 days of storage at 5 ◦C. Findings: At the end of storage, CaL treatment combined with HWT increased calcium (Ca) concentration, catalase activity, total phenolic content and radical scavenging activities (DPPH) of papaya slices, but decreased peroxidase, PG and PME activities compared to the control. In addition, these treatments reduced microbial growth compared to the control. Limitations: No limitations were encountered. Originality/Value: Both HWT with CaL showed potential for increasing markeabitity on fresh cut papaya with their generally recognized as safe status for consumers.