Background: Several infectious diseases have been found to be associated with transfusion of blood and blood components. Studies conducted in many African countries indicate a high prevalence of blood-borne pathogens such as Syphills infections among healthy blood donors.
 Objectives: This study conducted to determine the prevalence of syphills among blood donors and associated risk factors in Port Sudan city, 2016.
 Materials and methods: Across sectional laboratory-based study conducted in Port Sudan city. A Blood donor in central blood bank in Port Sudan during the period between August-October-2016. The data was collected through a structured questionnaire. Data was analyzed using SPSS version 15.0.
 Results: The prevalence of syphilis among blood donors were 13.24%. The risk factors associated with prevalence syphills were significantly the age group 40-49 years, hard workers, primary schools donors and blood donors who has two sex partnerships, p<0.05.
 Conclusion: The overall prevalence of syphills among blood donors in Portsudan city is 13.2% which is high in comparison to prevalence of noticed studies was done in different areas in the country. The risk factors associated with prevalence syphills were significantly the age group 40-49 years, hard workers, primary schools donors and blood donors who have two sex partnerships. Early and accurate detection of cases and conduction of effective treatment as to stop the progression of disease which can be infective to others. In addition to Health education on blood donations and safe sex behaviors targeting sexually active population, particularly between 20-50years old.