Variations in humidity can significantly disrupt the proper functioning of precision instruments and meters across various industrial, medical, and scientific applications, thereby necessitating real-time humidity monitoring. Here, a high sensitivity fiber-optic relative humidity (RH) sensor based on Mach-Zender interferometer (MZI) is proposed and demonstrated. The humidity sensor consists of single-mode fiber (SMF), peanut structure (PS), and multi-mode fiber (MMF). A multi-mode interference (MMI) enhanced fiber-optic MZI was constructed using a SMF-PS-SMF-MMF-SMF-PS-SMF cascade structure to detect small changes in the refractive index (RI) and expansion force of gelatin film caused by humidity variation. PS, as a beam splitter and combiner of MZI, provides the possibility for simple, economical, and efficient manufacturing of the fiber-based part of humidity sensors. The addition of MMF will excite higher-order core and cladding modes, laying the foundation for achieving high-sensitivity fiber-optic humidity sensors. The fiber-optic RH sensor prototype demonstrate a high sensitivity of about 0.633 nm/% RH in the RH range of 50 % RH to 80 % RH, and a low temperature cross-sensitivity of 0.165 % RH/°C. This sensor has the advantages of safe and easy manufacturing process, low manufacturing cost, and high sensitivity, providing continuous high accuracy humidity measurement results for humidity sensing in different fields.