Safety Applications Research Articles

Improvement of butt-fusion welding procedure and performance evaluation method for thick-walled bimodal polyethylene pipes

High-density polyethylene (HDPE) pipes have been continuously improved by the progress made by the resin industry and pipe manufacturers. Together with the advanced technology of welding equipment in terms of structure reliability and automations, HDPE pipes have been gradually applied to higher pressure level, bigger diameter, and thicker walls in operations which have become the preferred piping system for nuclear safety application in transporting cooling seawater. There are substantial differences in parameter settings when applying heat and force during different welding procedures, as well as the complicated evaluation methods for mechanical properties, which causes communication barriers between engineers and researchers. Thus, it is necessary to comprehensively study the welding procedures and evaluation methods to standardize the evaluation protocols in the industry. In this paper, based on the assumptions of temperature effect and molecular chain diffusion dynamics at the welding interface, the optimization of the welding procedure is proposed. Some well-established evaluation methods have been used for performance assessment and their feasibilities are discussed. The idea of creating crack along the weld interface is adopted as new method to evaluate the material ability of fracture resistance. Specimens with unilateral notch and weld interface crack are proposed and subjected for quasi-static axial tensile test. The fracture stress and fracture energy at the welding interface are used to differentiate the quality of the joint from different welding procedures. The fracture morphologies of the weld interface are analyzed. A complete picture is drawn for the mechanical fracture resistance of the weld interface. The method used in this paper can be corelated to the pipeline design conditions, therefore could be applied to the real-time engineering applications. Moreover, the evaluation method proposed in this paper is simple, reliable, and easy to implement.

Ultrasensitive and Highly Selective Detection of Staphylococcus aureus at the Single-Cell Level Using Bacteria-Imprinted Polymer and Vancomycin-Conjugated MnO2 Nanozyme.

Pathogenic bacterial infections, even at extremely low concentrations, pose significant threats to human health. However, the challenge persists in achieving high-sensitivity bacterial detection, particularly in complex samples. Herein, we present a novel sandwich-type electrochemical sensor utilizing bacteria-imprinted polymer (BIP) coupled with vancomycin-conjugated MnO2 nanozyme (Van@BSA-MnO2) for the ultrasensitive detection of pathogenic bacteria, exemplified by Staphylococcus aureus (S. aureus). The BIP, in situ prepared on the electrode surface, acts as a highly specific capture probe by replicating the surface features of S. aureus. Vancomycin (Van), known for its affinity to bacterial cell walls, is conjugated with a Bovine serum albumin (BSA)-templated MnO2 nanozyme through EDC/NHS chemistry. The resulting Van@BSA-MnO2 complex, serving as a detection probe, provides an efficient catalytic platform for signal amplification. Upon binding with the captured S. aureus, the Van@BSA-MnO2 complex catalyzes a substrate reaction, generating a current signal proportional to the target bacterial concentration. The sensor displays remarkable sensitivity, capable of detecting a single bacterial cell in a phosphate buffer solution. Even in complex milk matrices, it maintains outstanding performance, identifying S. aureus at concentrations as low as 10 CFU mL-1 without requiring intricate sample pretreatment. Moreover, the sensor demonstrates excellent selectivity, particularly in distinguishing target S. aureus from interfering bacteria of the same genus at concentrations 100-fold higher. This innovative method, employing entirely synthetic materials, provides a versatile and low-cost detection platform for Gram-positive bacteria. In comparison to existing nanozyme-based bacterial sensors with biological recognition materials, our assay offers distinct advantages, including enhanced sensitivity, ease of preparation, and cost-effectiveness, thereby holding significant promise for applications in food safety and environmental monitoring.

A novel electrochemical sensor for the detection of metronidazole residues in food samples

The widespread use and misuse of antibiotics in pharmaceuticals and animal farming has resulted in their accumulation in food sources and the environment, posing significant threats to human health, the environment, and the global economy. In this study, we have developed a hypersensitive, and ultra-selective electrochemical sensor, the first of its kind, by integrating a thermally annealed gold-silver alloy nanoporous matrix (TA-Au-Ag-ANpM) with reduced graphene oxide (r-GO) and poly(glycine) at the surface of a glassy carbon electrode (GCE). This sensor aims to detect life-threatening metronidazole (MTZ) residues in food samples. TA-Au-Ag-ANpM/r-GO/poly(glycine)/GCE was thoroughly characterized using a range of analytical techniques, including UV–Vis, FT-IR, XRD, SEM, and EDX. Furthermore, its electrochemical properties were investigated by cyclic voltammetry (CV), square wave voltammetry (SWV), and electrochemical impedance spectroscopy (EIS). The sensor exhibited outstanding performance, with a broad linear range of 2.0 pM–410 μM. The limits of detection (LOD) and quantification (LOQ) were determined to be 0.0312 pM and 0.104 pM, respectively. The TA-Au-Ag-ANpM/r-GO/poly(glycine)/GCE exhibited exceptional reproducibility, repeatability, stability, and resistance to interferences. Moreover, the sensor demonstrated outstanding performance in detecting MTZ residues in milk powder, pork, and chicken meat samples, achieving very good recoveries (96.9%–101.4%) with a relative standard deviation (RSD) below 5%. This performance highlights the potential for practical applications in food safety and quality monitoring. Therefore, the developed sensor contributes to the advancement of electrochemical sensing technology and its application in ensuring food safety and integrity by combating antibiotic residues.

Liquid-Phase Exfoliated Graphene and Polytetrafluoroethylene for Highly Durable and Reusable Chemical Leak Detection Sensors

Graphene-based chemical sensors hold promise across diverse applications owing to their exceptional sensitivity and selectivity. However, achieving their long-term durability and reusability while preserving high sensitivity remains a significant challenge, particularly in harsh environments where exposure to strong chemicals is inevitable. This paper presents a novel approach to address this challenge by synergistically integrating liquid-phase exfoliated graphene (LPEG) with polytetrafluoroethylene (PTFE) within a single sensing strip. Through a comprehensive experimental investigation, we demonstrate the fabrication of highly durable and reusable chemical leak detection sensors by combining LPEG and PTFE. Furthermore, we explore the sensing mechanism, highlighting the roles of LPEG and PTFE in enhancing sensitivity and selectivity, along with durability and reusability. Performance evaluation reveals the sensors’ robustness against mechanical and chemical degradation, coupled with excellent recyclability. This innovative approach holds promise for applications in environmental monitoring, industrial safety, and healthcare, thus advancing the field of graphene-based chemical leak detection sensors.

Synthesis and antitumor activity of dolutegravir derivatives bearing 1,2,3-triazole moieties

Modification of marketed drugs is an important way to develop drugs because its safety and clinical applicability. Oxygen-nitrogen heterocycles are a class of important active substances discovered in the process of new drug development. Dolutegravir, an HIV drug with a nitrogen-oxygen heterocycle structure, has the potential ability to inhibit cell survival. In order to find and explore novel anti-tumor drugs, new dolutegravir derivatives bearing different 1,2,3-triazole moieties were prepared via click reactions. In vitro biological experiments performed in several lung cancer cell lines suggested that these novel compounds displayed potent anti-tumor ability. Especially, the compound 9e with a substituent of 2-methyl-3-nitrophenyl and the compound 9p with a substituent of 3-trifluoromethylphenyl were effective against PC-9 cell line with IC50 values of 3.83 and 3.17 µM, respectively. Moreover, compounds 9e and 9p were effective against H460 and A549 cells. Further studies suggested that compounds 9e and 9p could induce cancer cell apoptosis in PC-9 and H460, inhibit cancer cell proliferation, change the cell cycle, and increase the level of reactive oxygen species (ROS) which further induce tumor cell apoptosis. In addition, compounds 9e and 9p increased LC3 protein expression which was the key regulator in autophagy signaling pathway in PC-9 cells. Compound 9e also showed low toxicity against normal cells, and could be regarded as an interesting lead compound for further structure optimization.

An enhanced method for evaluating the effectiveness of protective devices for road safety application

This paper presents an enhanced probabilistic approach to estimate the real-world safety performance of new device concepts for road safety applications from the perspective of Powered Two-Wheeler (PTW) riders who suffer multiple injuries in different body regions. The proposed method estimates the overall effectiveness of safety devices for PTW riders by correlating computer simulations with various levels of actual injuries collected worldwide from accident databases. The study further develops the methodology initially presented by Johnny Korner in 1989 by introducing a new indicator, Global Potential Damage (GPD), that overcomes the limitations of the original method, encompassing six biomechanical injury indices estimated in five body regions. A Weibull regression model was fit to the field data using the Maximum Likelihood Method with boundaries at the 90% confidence level for the construction of novel injury risk curves for PTW riders. The modified methodology was applied for the holistic evaluation of the effectiveness of a new safety system, the Belted Safety Jacket (BSJ), in head-on collisions across multiple injury indices, body regions, vehicle types, and speed pairs without sub-optimizing it at specific crash severities. A virtual multi-body environment was employed to reproduce a selected set of crashes. The BSJ is a device concept comprising a vest with safety belts to restrict the rider’s movements relative to the PTW during crashes. The BSJ exhibited 59% effectiveness, with an undoubted benefit to the head, neck, chest, and lower extremities. The results show that the proposed methodology enables an overall assessment of the injuries, thus improving the protection of PTW users. The novel indicator supports a robust evaluation of safety systems, specifically relevant in the context of PTW accidents.

Ammonia-trapping multilayer polymer urease film amplifier coated surface plasmon resonance sensor for ultra-sensitive urea detection

In this paper, we have developed a new urea signal amplification technology based on a multilayer urease polymer film self-assembly structure for the first time, enabling the realization of ultra-accurate urea optical detection. We demonstrate the effectiveness of this technique based on surface plasmon resonance (SPR) platform. When the urea signal amplifier is combined with SPR sensors, multilayer urease polymer film contacts with urea molecules, the urea molecules are oxidated into NH3 and CO2, causing the pH increase, which weakens the electrostatic interaction between different polymer layers and induces the RI reduction of the multilayer urease film, thereby causing the shift of the SPR spectrum. Experiment results confirm that urea sensitivity has been improved 12-fold, and the limit of detection (LOD) has been optimized to less than 10−3 mmol/L. Furthermore, the anti-interference has been improved 22.8-fold and is kept even the interference signal strength increases by 100 times. Moreover, multilayered urease polymer leads to the increase of urease on the sensor surface, thus expanding the detection range of urea by 3.5 times. This provides a new way to solve the contradiction between high sensitivity and large measurement range of optical fiber biosensor. Importantly, the concept of using enzyme-loaded polymer films for signal amplification can be adapted for the detection of various other biomolecules by replacing the enzyme with a specific recognition element for the target molecule. This opens doors for developing highly sensitive and specific biosensors for diverse applications in healthcare, environmental monitoring, and food safety.

Fuzzy-AHP based optimal RSU deployment (Fuzzy-AHP-ORD) approach using road and traffic analysis in VANET

Vehicular Ad Hoc Network (VANET) is a proven technology in Intelligent Transportation Systems (ITS) that provides various safety and non-safety applications. The Roadside Unit (RSU) is one of the essential components of the VANET, which allows vehicles to disseminate and exchange safety and infotainment information in a broader range. RSUs are also valuable for offloading the internet data traffic of vehicular users from cellular networks. However, due to high deployment and maintenance costs and security overhead, it is essential to optimally deploy these RSUs and place them in prominent places to increase the overall network coverage and efficiency. The existing deployment strategies either analyzed the road network on one or a few topological parameters or vehicular traffic parameters and considered equal or random weights for each parameter. None of them deal with the uncertainty of parameter weightage. Thus, this paper proposes a Fuzzy-Analytic Hierarchy Process-based optimal RSU deployment (Fuzzy-AHP-ORD) approach to finding the highly influential intersection nodes for RSU deployment. The Fuzzy-AHP-ORD considers different static and dynamic parameters concerning road topology and the vehicle’s traffic behavior. The weights of the parameter are calculated through Fuzzy-AHP, then the various intersection nodes of the road networks are ranked using the TOPSIS (Technique for Order of Preference by Similarity to Ideal Solution) based MADM (Multiple Attribute Decision Making) method. The simulation results reveal the effectiveness of the proposed method in comparison to other benchmark methods in terms of coverage ratio, connection time, packet delivery ratio, and delay.

Cardiovascular adverse events associated with antibody-drug conjugates (ADCs): a pharmacovigilance study based on the FAERS database.

As a novel drug formulation, antibody drug conjugates (ADCs) are widely used in various types of cancer. However, clinically, there is a lack of attention to the CVD produced by them, as well as a lack of research on the real-world situation. Using the Food and Drug Administration Adverse Event Reporting System (FAERS) database, to ensure its clinical safety application, we analyzed post-marketing data on antitumor ADCs to identify risk factors and drugs associated with the risk of cardiovascular events. We used OpenVigil 2.1 to conduct a database query for adverse events (AEs) reported to the FAERS database between the time the drug was launched and the second quarter of 2023. Cardiovascular adverse events (AEs) were grouped into fourteen narrow categories using the Standardized Medical Dictionary for Regulatory Activities (MedDRA) Queries (SMQs), and the reporting odds ratio (ROR) and the proportional reporting ratio (PRR) for reporting the association between different drugs and cardiovascular disease (CVD) risk were calculated. In the FAERS database, 1863 AEs associated with CVD we studied were identified in patients receiving ADC therapy. Most reports came from people aged ≥65, but a significant number of cases were found to be unknown. The number of patients with antibody-drug conjugates (ADCs)-related CVD cases aged <18years, 18-64years, and≥ 65years was 52 (2.79%), 586 (31.45%), and 613 (32.90%), respectively. The proportion of female patients (834, 44.77%) was higher than that of male patients (752, 40.37%). Death (770 reports), disability (9 reports), Hospitalization initial or prolonged (407 reports), and life-threatening reactions (187 reports). Of the 770 deaths reported, 103 (31.7%) were associated with brentuximab vedotin, 10 (24.4%) with sacituzumab govitecan, 22 (19.3%) with enfortumab vedotin, and 35 (34.7%) with trastuzumab emtansine.49 (41.2%) cases were associated with polatuzumab vedotin, 62 (29%) with trastuzumab deruxtecan, 423 (54.3%) with gemtuzumab ozogamicin, and 66 (38.8%) with inotuzumab ozogamicin. In a disproportionate number of SMQS, cardiac failure (n = 277) and embolic and thrombotic events, venous (n = 446) were the most frequently reported CVD-related AEs in ADCs. By mining the FAERS database, we provided relevant information on the association between ADC use and cardiovascular-associated AEs. ADCs were associated with increased cardiovascular toxicity, deserving distinct monitoring and appropriate management. Further research is needed to confirm these findings and assess causality.

A novel method for cycle slip detection and restoration of BDS carrier phase

The chief purpose of this present study is to develop a new approach to cycle slip detection and restoration of the BeiDou Navigation Satellite System (BDS) carrier phase. Based on the RAIM algorithm in the application of aviation safety, we extend the application to ensure the precision of the carrier phase. To stand-alone receivers of a low-cost single frequency, carrier phase data is made different between adjacent epochs. Then least-square residuals were to design a RAIM scheme with differential carrier phase data to find out the epoch with cycle slip, and finally repair cycle slip by Chebyshev polynomial. Results indicate that the approach can detect cycle slip effectively and both big cycle slip and small cycle slip could be repaired.

Impact of gamma rays on DNA quality and genetic traits in human blood samples

This research investigates the effects of gamma rays on DNA quality and genetic traits in eight participants from Saudi Arabia (4 males and 4 females). The study aims to understand changes in genetic fingerprint sites resulting from varying levels of radiation exposure. It addresses three key research questions related to DNA quantity, obtaining complete genetic fingerprint sites, and the reliability of genetic trait results from cancer patients undergoing radiation therapy. The study's significance lies in its potential applications in forensics, medical diagnostics, and public safety, providing insights into identifying victims and perpetrators in radiation-related incidents and enhancing treatment planning for cancer patients exposed to radiation therapies. The research contributes to a broader understanding of radiation's impact on genetic material, promotes scientific knowledge, and safeguards public health. This research investigated the impact of gamma radiation on DNA quality and genetic traits in human blood samples from Saudi Arabian volunteers. Exposure to gamma radiation at doses similar to therapeutic levels used in cancer treatment was found to cause DNA fragmentation, resulting in decreased concentrations of detectable genetic loci. The study revealed that higher radiation doses had a more pronounced effect on DNA content in blood cells.

Microfluidic paper analytic device (μPAD) technology for food safety applications.

Foodborne pathogens, food adulterants, allergens, and toxic chemicals in food can cause major health hazards to humans and animals. Stringent quality control measures at all stages of food processing are required to ensure food safety. There is, therefore, a global need for affordable, reliable, and rapid tests that can be conducted at different process steps and processing sites, spanning the range from the sourcing of food to the end-product acquired by the consumer. Current laboratory-based food quality control tests are well established, but many are not suitable for rapid on-site investigations and are costly. Microfluidic paper analytical devices (μPADs) are a fast-growing field in medical diagnostics that can fill these gaps. In this review, we describe the latest developments in the applications of microfluidic paper analytic device (μPAD) technology in the food safety sector. State-of-the-art μPAD designs and fabrication methods, microfluidic assay principles, and various types of μPAD devices with food-specific applications are discussed. We have identified the prominent research and development trends and future directions for maximizing the value of microfluidic technology in the food sector and have highlighted key areas for improvement. We conclude that the μPAD technology is promising in food safety applications by using novel materials and improved methods to enhance the sensitivity and specificity of the assays, with low cost.

Development and application of Tractebel's multi-physics simulation capacity for advanced reactor safety evaluation

This paper provides a comprehensive overview of Tractebel’s current capabilities in transient simulation of Pressurized Water Reactors (PWRs) using multi-physics approaches. It highlights the use of advanced, industry-grade simulation tools and methodologies to tackle practical engineering and licensing studies for PWRs. The paper first introduces the dedicated coupling environment and its recent advancements, which have culminated in the CERES Multiphysics platform. It then delves into three key safety application areas where Tractebel’s multi-physics simulation capabilities are applied, with a focus on system transient analysis:•Small Modular Reactor (SMR) R&D Activities: Tractebel’s contribution to the Horizon 2020 McSAFER project is discussed.•Plant Safety Analyses: The paper discusses studies conducted within the framework of the WENRA RL DEC-A safety analyses.•Plant Operational Support: The paper details how multi-physics simulations are used to justify plant restarts after outages as a preventive measure against potential technical specification violations.The paper also outlines future developments aimed at enhancing and applying these multi-physics simulation capabilities to support new plant designs, including SMRs.

The Design of a Speech Recognition-Based Finger Training Rehabilitation Device: Mechanical and System Aspects

Aiming to address the extended diagnosis and recovery period along with low efficiency in traditional stroke patient rehabilitation, this study introduces a speech recognition-based finger rehabilitation training control system. This system enables patients to perform finger exercises while providing feedback on finger angle, speed, and position. Furthermore, it offers rehabilitation physicians valuable data for evaluation and reference in finger rehabilitation. The system is divided into hardware circuitry, a lower computer control system, and a voice recognition human-computer interaction system, all working in conjunction with the finger movement perception system. By applying the Hidden Markov Model (HMM) algorithm to the voice interaction system for pattern matching, the finger rehabilitation system undergoes simulation testing. The results demonstrate that the proposed rehabilitation training system based on speech recognition meets design requirements, ensuring safety, reliability, and substantial application potential in future finger rehabilitation training.

Potential powered EC-SERS for sensitive detection of acetamiprid

Surface-enhanced Raman spectroscopy (SERS) is a powerful tool for rapid and sensitive detection of pesticide residues in food, and the applied potential regulated SERS technique can be endowed with a higher sensitivity. In this work, we developed an electrochemical-surface enhanced Raman spectroscopy (EC-SERS) sensor for the rapid detection of acetamiprid (AAP) in vegetables. AuNPs/ITO were prepared via in-situ electrodeposition of gold nanoparticles (AuNPs) on an indium tin oxide (ITO) both as a SERS substrate and working electrode. By adding AAP into an in-situ Raman electrolytic cell with an applied electrochemical potential (+0.3 V), the SERS intensity was enhanced by 4-fold and the characteristic signal was linearly related to the concentration of AAP. Under the optimal conditions, the EC-SERS method exhibited a linear detection range from 0.05 to 50 μM, with a limit of detection (LOD, 3S/M) of 0.02 μM. Simultaneously, based on density functional theory (DFT), the adsorption characteristics of AAP on AuNPs/ITO were studied in the presence of electric field. The method was finally confirmed by HPLC-MS for the detection of real samples, revealing its potential application in food safety.

Consumer meat safety knowledge and hygiene practices in a veterinary disease-controlled area of South Africa

Consumers’ practices and knowledge play a significant role in ensuring meat safety and hygiene application. This is important in an environment where the slaughter and release of such meat is restricted and not always controlled and monitored. This could have been contributed by the lack of slaughter facilities; the controlled movement of live animal restrictions applicable in a typical veterinary diseases-controlled area. The aim of this investigation was to identify meat hygiene practices and general meat safety knowledge applied by consumers of central Bushbuckridge, South Africa. Structured interviews (n=81) were conducted with consumers observed purchasing meat from local meat supply centres. Data analysis was achieved by SAS Statistical software (Version 26) SAS and graphs were drawn using MS Excel 2020. The majority (84.2%, σ=29.4) of the consumers indicated that meat supplied in the region were from registered facilities. The general belief amongst the consumers was that routine hygiene inspection was conducted by authorities at all meat distributing or butchery facilities (74.1%, σ=39.7). None of the facilities or/and butcheries in the study area was registered and therefore, no formal regulated practices could have been conducted. Slaughter and distribution of meat within the area were seen as a good business by 78.6%, σ=18.9 of the consumers, and as a result, the consumers did not have a problem with widespread slaughter and release of this meat to the public outside of those facilities. The extent of hygiene application practices at household level varied. These included the assurance that perishable products were properly handled after purchase (84.1%, σ=8.3), and the handling or transportation of sourced meat did not comply with cold chain management prescripts (39.9%, σ=44.9). The likelihood of consumers feeling the same about meat hygiene concerns and practices is undoubtedly low as represented by a high standard deviation (σ) of the responses. These results show that consumers were relying on authorities for meat safety assurances. This ought to caution authorities on consumer practices and needed interventions such as animal movement policies enforcement. The adoption of such policies by consumers lies with the development and rollout of consumer awareness and meat safety assurances training programs. Key words: abattoir, consumer practices, illegal slaughter, meat safety, meat hygiene

Exploring the efficacy of Congo Red dye as a corrosion inhibitor for aluminum in HCl solution: An interdisciplinary study with RSM modeling and theoretical simulations

This study investigates Congo Red Textile Dye (CRD) as a corrosion inhibitor for aluminum in a 1.0 mol/L hydrochloric acid solution. Utilizing a comprehensive suite of analytical techniques, including gravimetric analysis, scanning electron microscopy (SEM), ultraviolet–visible spectrophotometry (UV–Vis), Electrochemical Impedance Spectroscopy (EIS), and Potentiodynamic Polarization curves (PDP), we assessed CRD’s adsorption mechanism and its corrosion inhibition effectiveness. Significant corrosion mitigation was observed, with the efficiency of inhibition EI (%) increasing alongside CRD concentration, achieving a maximum efficiency of 87.5 %. This enhancement in EI (%) decreases with rising temperatures, assessed across a range from 298 to 323 K. Response surface methodology (RSM) and central composite design (CCD) were employed for accurate corrosion rates (Vcorr) and EI (%) prediction. Additionally, theoretical analysis was performed using global and local reactivity descriptors along with molecular dynamics (MD) simulations. Theoretical results confirmed the widespread presence of nucleophilic and electrophilic sites, along with a strong affinity for the Al surface, suggesting CRD’s potential for enhancing corrosion resistance. This study proposes a potential pathway for the valorization of Congo Red dye, utilizing concentrations within non-toxic thresholds, thereby aligning with environmental safety and industrial application considerations.

Biochemical and molecular-level effects of co-exposure to chlorpyrifos and lambda-cyhalothrin on the earthworm (Eisenia fetida)

Farmland soil organisms frequently encounter pesticide mixtures presented in their living environment. However, the underlying toxic mechanisms employed by soil animals to cope with such combined pollution have yet to be explored. This investigation aimed to reveal the changes in cellular and mRNA levels under chlorpyrifos (CPF) and lambda-cyhalothrin (LCT) co-exposures in earthworms (Eisenia fetida). Results exhibited that the combination of CPF and LCT triggered an acute synergistic influence on the animals. Most exposures resulted in significant alterations in the activities of total superoxide dismutase (T-SOD), copper/zinc superoxide dismutase (Cu/Zn-SOD), caspase 3, and carboxylesterase (CarE) compared to the basal level. Moreover, when exposed to chemical mixtures, the transcription levels of four genes [heat shock protein 70 (hsp70), gst, sod, and calreticulin (crt)] also displayed more pronounced changes compared with their individual exposures. These changes in determined parameters indicated the occurrence of oxidative stress, cell death, detoxification dysfunction, and endoplasmic reticulum damage after co-exposure to CPF and LCT in E. fetida. The comprehensive examination of mixture toxicities of CPF and LCT at different endpoints would help to understand the overall toxicity they cause to soil invertebrates. The augmented deleterious effect of these pesticides in a mixture suggested that mixture toxicity assessment was necessary for the safety evaluation and application of pesticide mixtures.

Benefits of l-theanine supplementation and its possible role in clinical use – review

Introduction: Green tea has been a meaningful element of various cultures among the world for ages and its benefits for human health are widely known. Discovery of its ingredients facilitated the profound research of their positive role and mechanisms of action. One of them – l-theanine - seems to have a multidirectional influence on human health. Methods and materials: A review of chosen literature from PubMed database, GoogleScholar database between the years 2000-2023 was conducted using the following keywords: “theanine”, “theanine cognition”, “theanine brain activity”, “theanine neural activity”, “theanine neuroprotection”, “theanine stress”, “theanine cancer”, “theanine immunity”, “theanine safety”, “theanine caffeine”, “theanine metabolism” Aim of study: Review of current knowledge on l-theanine, its benefits for health, mechanisms of action, possible applications in clinical use and safety. Results: L-theanine has a profitable influence on cognitive performance, stress management, neuroprotection, immunity and exhibits anti-cancer properties while having virtually no detrimental side effects. Conclusions: Further research on application of l-theanine in clinical use is necessary as studies carried out on humans included only a limited number of participants.

Benefits of l-theanine supplementation and its possible role in clinical use – review

Introduction: Green tea has been a meaningful element of various cultures among the world for ages and its benefits for human health are widely known. Discovery of its ingredients facilitated the profound research of their positive role and mechanisms of action. One of them – l-theanine - seems to have a multidirectional influence on human health. Methods and materials: A review of chosen literature from PubMed database, GoogleScholar database between the years 2000-2023 was conducted using the following keywords: “theanine”, “theanine cognition”, “theanine brain activity”, “theanine neural activity”, “theanine neuroprotection”, “theanine stress”, “theanine cancer”, “theanine immunity”, “theanine safety”, “theanine caffeine”, “theanine metabolism” Aim of study: Review of current knowledge on l-theanine, its benefits for health, mechanisms of action, possible applications in clinical use and safety. Results: L-theanine has a profitable influence on cognitive performance, stress management, neuroprotection, immunity and exhibits anti-cancer properties while having virtually no detrimental side effects. Conclusions: Further research on application of l-theanine in clinical use is necessary as studies carried out on humans included only a limited number of participants.