Background: The sacroiliac (SI) joint has been found to be a source of discomfort for 25 to 35% of people sufferingfrom persistent low back pain. Though the Gluteus Maximus and Hamstring muscles play a vital role in sit-tostandactivity in normal people, its role in individuals with sacroiliac joint dysfunction is yet to be studied.Purpose: To assess the association between Gluteus Maximus activity and Hamstring muscle length and determinethe efficacy of Gluteus Maximus activation exercise and Hamstring muscle eccentric training on improving sit-tostandactivity in people with SI Joint dysfunction.Materials and Methods: In the first phase of the study, Hamstring muscle length, Gluteus maximus muscleactivity, Pain during sit-to-stand activity was recorded. All thirty participants in the second phase of the studyunderwent six weeks of Hamstring muscle eccentric exercise and Gluteus Maximus muscle activation exercise.Results: A Pearson correlation test shows a positive correlation (r = 0.208) but the weaker relationship between thevariables. Paired t test analysis shows a significant improvement after the treatment in terms of Gluteus Maximusmuscle activity, Hamstring muscle length and pain reduction with a P value < 0.001.Conclusion: This study concluded that Gluteus Maximus activation exercise and Hamstring muscle eccentrictraining will significantly improve sit to stand activity and reduce pain.