Repetitive head impacts (RHI) are the result of a blow to the head that does not elicit clinical signs or symptoms of a concussion. Recent evidence suggests that RHI from a single season of collegiate football can lead to a reduction in midbrain white matter integrity. The midbrain carries projecting fibers to the trochlear and oculomotor nerves, which if damaged may impair oculomotor control. PURPOSE: The purpose of this study was to evaluate oculomotor function following multiple years of Division I contact sports during a dynamic visual acuity (DVA) task. METHODS: Two NCAA Division I football defensive backs with no diagnosed concussion history (a first-year freshmen [F1; age=18 years], senior [S1; age=21 years]) and a healthy control (CON; age=23 years), all with lower than 20/20 vision, completed a DVA task at pre-season. For the DVA task (optotype spatial range=1.0 to -0.3), participants were asked to complete 60 randomized trials of smooth pursuit (30°/s) and saccades (150°/s). Participants head were stabilized in a chin rest at a distance of 154cm away from the 26° visual field monitor (165Hz, 2560 x 1440 pixel resolution, 300 cd/m2 luminance) while wearing a head-mounted binocular video oculography eye tracker (Eyelink SR research, 500 Hz, Ottawa, CN). Using a 2-up-1-down staircase method, participants tracked a Landolt-C ring that moved across the screen (horizontally left to right) where the size of the gap in the C along with the orientation (left, right, up or down) adjusted based on the correct/incorrect responses during both smooth pursuit and saccadic trials. Smooth pursuit eye movement (SPEM) velocity gain and saccadic peak velocity were calculated using ternary eye movement classification from the transformed spherical coordinates via a custom MATLAB code (MATLAB 2019a, Natick, MA, USA). No statistical analysis were performed given the single-subject design. RESULTS: SPEMs gain is lower for S1 (0.88) when compared to F1 (0.82) and CON (0.92). Similarly, during the saccadic trials, S1 had slower average saccadic peak velocity (S1=279.75°/s; F1 =392.34°/s; CON=491.96°/s). CONCLUSIONS: These results may indicate that engaging in contact collision sport for 2+ years at the Division 1 level may result in less accurate (lower SPEMs gain) and slower saccadic eye movements. Supported by NIH P20GM103650