Sequence S1 Research Articles

Characteristics and genesis of the Zhongnan Fault Zone in the South China Sea oceanic basin: Insights from an integrated analysis of multibeam bathymetric and 2D multichannel seismic data

The Zhongnan Fault Zone (ZFZ) is a large-scale tectonic belt in the South China Sea (SCS) oceanic basin and plays an important role in the formation and evolution of the basin. Despite numerous studies over the past few decades, debates persist regarding its location, orientation, nature, time of activity, and genesis. In this study, we evaluate our findings on the characteristics and origin of the fault zone through an integrated analysis of multibeam bathymetric and 2D multichannel seismic data. Our results reveal the ZFZ as a fault zone approximately 400 km long and 50–90 km wide, situated between the east subbasin (ESB) and southwest subbasin (SWSB) of the SCS. The fault zone is oriented N8°W, roughly perpendicular to and laterally displacing the relict spreading center and related spreading lineaments. Bounded by discontinuous linear seamounts, the fault zone comprises two V-shaped subparallel pull-apart basins and a separating basement high. Steeply dipping (>60°) normal basement-involved faults bound these pull-apart basins, forming typical negative flower structures. Numerous northeast-oriented en-echelon linear bathymetric highs within the fault zone are identified as secondary antithetic shears (P′ shears). These shears are characterized by their orientation relative to the principal displacement zones defined along the pull-apart basins. The ZFZ exhibits differences from the adjacent subbasins in water depth, basement burial, stratal thickness, and seismic stratigraphic characteristics. Five seismic sequences (S1–S5 upward) in the ZFZ and nearby ESB and SWSB are defined, dating to the Early Miocene synspreading (S1) and the Middle Miocene to Recent postspreading (S2–S5) stages, respectively. The difficulty in correlating seismic facies in sequences S1–S3, compared with the comparable seismic facies in sequences S4–S5 between the ZFZ and adjacent subbasins, suggests a horizontal displacement during the synspreading and early postspreading stages. We propose that the ZFZ functioned as a right-lateral transform fault zone during the synspreading period (the Early Miocene, approximately 24–16 Ma) of the SWSB and transitioned into a left-lateral strike-slip fault zone during the successive early postspreading period (the Middle-Late Miocene, approximately 16–5.3 Ma).

A Class of Bounded Iterative Sequences of Integers

In this note, we show that, for any real number τ∈[12,1), any finite set of positive integers K and any integer s1≥2, the sequence of integers s1,s2,s3,… satisfying si+1−si∈K if si is a prime number, and 2≤si+1≤τsi if si is a composite number, is bounded from above. The bound is given in terms of an explicit constant depending on τ,s1 and the maximal element of K only. In particular, if K is a singleton set and for each composite si the integer si+1 in the interval [2,τsi] is chosen by some prescribed rule, e.g., si+1 is the largest prime divisor of si, then the sequence s1,s2,s3,… is periodic. In general, we show that the sequences satisfying the above conditions are all periodic if and only if either K={1} and τ∈[12,34) or K={2} and τ∈[12,59).

A screen for regeneration-associated silencer regulatory elements in zebrafish

Regeneration involves gene expression changes explained in part by context-dependent recruitment of transcriptional activators to distal enhancers. Silencers that engage repressive transcriptional complexes are less studied than enhancers and more technically challenging to validate, but they potentially have profound biological importance for regeneration. Here, we identified candidate silencers through a screening process that examined the ability of DNA sequences to limit injury-induced gene expression in larval zebrafish after fin amputation. A short sequence (s1) on chromosome 5 near several genes that reduce expression during adult fin regeneration could suppress promoter activity in stable transgenic lines and diminish nearby gene expression in knockin lines. High-resolution analysis of chromatin organization identified physical associations of s1 with gene promoters occurring preferentially during fin regeneration, and genomic deletion of s1 elevated the expression of these genes after fin amputation. Our study provides methods to identify "tissue regeneration silencer elements" (TRSEs) with the potential to reduce unnecessary or deleterious gene expression during regeneration.

Development and validation of an RT-qPCR for detection and quantitation of emerging epizootic hemorrhagic disease virus serotype 8 RNA from field samples

Epizootic hemorrhagic disease virus (EHDV) is a Culicoides-transmitted virus circulating in multiple serotypes. It has become a concern in the European Union as a novel strain of the serotype 8 (EHDV-8) of clear Northern African origin, has been recently discovered in symptomatic cattle in Italy (islands of Sardinia and Sicily), Spain, and Portugal. Current molecular typing methods targeting the S2 nucleotide sequences -coding for the outermost protein of the virion VP2- are not able to detect the novel emerging EHDV-8 strain as they enrolled the S2 sequence of the unique EHDV-8 reference strain isolated in Australia in 1982. Thus, in this study, we developed and validated a novel typing assay for the detection and quantitation of the novel EHDV-8 RNA from field samples, including blood of ruminants and insects. This molecular tool will certainly support EHDV-8 surveillance and control.

Volcanosedimentary fill of the early Cretaceous Yingcheng formation and response to the end of continental rifting in the Songliao Basin: Constraints from well SK-2, northern Xujiaweizi fault depression

Well SK-2 of the International Continental Scientific Drilling Project (ICDP) obtained continuous core samples with a length of 365.32 m in the Early Cretaceous Yingcheng Formation, showing a stratigraphy of interbedded rhyolites and coarse clastic rocks in the Xujiaweizi fault depression. The Yingcheng Formation in well SK-2 is divided into three sequences. Sequences 1 (S1) and 3 (S3) consist of coarse clastic rocks, and four types of conglomerate lithofacies are identified. The conglomerate lithofacies of S1 is characterized by gravity flows. In comparison, the conglomerate lithofacies of S3 is characterized by a progradational braided channel, reflecting a proximal provenance and a shallowing upward setting. Sequence 2 (S2) rocks are rhyolites, and geochemical analysis shows these to have mainly originated from the middle-upper crust. On the basis of volcanic rocks in wells Shengshen 2–1 and Shengshen 203 and seismic profiles crossing these wells, we identify two types of volcanic edifices: stratovolcanoes in the east and shield volcanoes in the west. The Early Cretaceous strike-slip faults, including the Xuzhong and Xudong faults, play an important role in controlling this area. In comparison with extensive, dark, and fine-grained clastic rocks of the Shahezi Formation, the felsic and coarse clastic rocks of the Yingcheng Formation in well SK-2 mark a sedimentary response to strike-slip tectonics and indicate the ending fault movement of the Xujiaweizi fault depression, and perhaps even the end of the Early Cretaceous Songliao Basin rifting stage.

SARS-CoV-2 omicron variants harbor spike protein mutations responsible for their attenuated fusogenic phenotype

Since the emergence of the Omicron variants at the end of 2021, they quickly became the dominant variants globally. The Omicron variants may be more easily transmitted compared to the earlier Wuhan and the other variants. In this study, we aimed to elucidate mechanisms of the altered infectivity associated with the Omicron variants. We systemically evaluated mutations located in the S2 sequence of spike and identified mutations that are responsible for altered viral fusion. We demonstrated that mutations near the S1/S2 cleavage site decrease S1/S2 cleavage, resulting in reduced fusogenicity. Mutations in the HR1 and other S2 sequences also affect cell-cell fusion. Based on nuclear magnetic resonance (NMR) studies and in silico modeling, these mutations affect fusogenicity possibly at multiple steps of the viral fusion. Our findings reveal that the Omicron variants have accumulated mutations that contribute to reduced syncytial formation and hence an attenuated pathogenicity.

On the direct and inverse zero-sum problems over Cn⋊sC2

Let Cn be the cyclic group of order n. In this paper, we provide the exact values of some zero-sum constants over G=Cn⋊sC2 where s≢±1(modn), namely η-constant, Gao constant, and Erdős-Ginzburg-Ziv constant (the latter for all but a “small” family of cases). As a consequence, we prove the Gao's and Zhuang-Gao's Conjectures for groups of this form. We also solve the associated inverse problems by characterizing the structure of the sequences S1,S2,S3 over G of maximal lengths such that S1 has no short product-one subsequences, S2 has no product-one subsequences of length exp⁡(G), and S3 has no product-one subsequences of length |G|.

IS26-Mediated Formation of a Hybrid Plasmid Carrying mcr-1.1.

The objective of this study was to elucidate the characteristics and mechanism of formation of the fusion plasmid pHNSHP24 carrying mcr-1.1. mcr-1.1-bearing Escherichia coli SHP24 and the corresponding transconjugant were subjected to whole-genome sequencing (WGS) combining the Illumina and MinION platforms to obtain the complete sequences of the fusion plasmid and its original plasmids. Complete sequence analysis and S1 nuclease-pulsed field gel electrophoresis (S1-PFGE) results indicated that E. coli SHP24 carried four plasmids: mcr-1.1-harboring phage-like plasmid pHNSHP24-3, F53:A-:B- plasmid pHNSHP24-4, pHNSHP24-1, and pHNSHP24-2. However, the plasmid pHNSHP24 carrying mcr-1.1 presents in the transconjugant differed from the four plasmids in the donor strain SHP24. Further analysis showed that pHNSHP24 may be the fusion product of pHNSHP24-3 and pHNSHP24-4 and is formed through a replicative transposition mechanism mediated by IS26 in E. coli SHP24. This study is the first to report the fusion of an mcr-1.1-harboring phage-like pO111 plasmid and an F53:A-:B- plasmid mediated by IS26. Our findings revealed the role of phage-like and fusion plasmids in the dissemination of mcr-1.1.

Sedimentology and stratigraphy of the Cenomanian Buda Limestone in central Texas, U.S.A.: Implications on regional and global depositional controls

The Cenomanian Buda Limestone is a distinctive rock formation widely distributed in Texas and its neighboring regions. The extensive character and regional significance of this unit make the Buda Limestone a key stratigraphic datum in the Texas Gulf Coast Basin. Detailed sedimentologic and stratigraphic studies of this formation were mostly focused on the west Texas exposures. In central Texas, on the contrary, very few studies have been performed. Thus, the sedimentary environments and depositional dynamics in this region are still poorly understood. Moreover, the regional correlation between the central and west Texas lithostratigraphic members of this formation remains unknown. Here, based on a detailed sedimentologic and stratigraphic study of central Texas sections (Austin-Kingsbury-San Antonio transect), the Buda Limestone is interpreted as a shallow-subtidal, semi-restricted to nearly open marine (lagoonal) shelf deposit. This unit is subdivided into two members (comprising 4th-order sequences S1 and S2) laterally variable in the study area. Vertical and lateral facies changes in the Buda Limestone are interpreted to be a function of the dynamic interaction of an irregular topography with fluctuating sea levels, under possible eustatic control. The informal (central Texas) “lower Buda” member is laterally correlated with the Lechuguilla Member (lower Buda) and Red Light Member (middle Buda) of western Texas. The informal (central Texas) “upper Buda” member, on the other hand, corresponds to the Love Station Member (upper Buda) from west Texas.

Periodic Patchy Spheres Self-Assembled by AmBCAn' Multiblock Terpolymers.

We have designed AmBCAn' multiblock terpolymers and studied their self-assembly using self-consistent field theory, aiming to generate the periodically arranged patchy spheres and thus to clarify the regulation mechanism of the number of patches. A number of two-dimensional phase diagrams are constructed for three typical architectures A2BCA2', A2BCA3', and A3BCA2'. Four kinds of stable patchy spheres with the number of patches as 2 (S2), 4 (S4), 5 (S5), and 6 (S6) are obtained. These phases follow a common transition sequence of S2 → S4 → S5 → S6 along with the increasing of the volume fraction of C-block (fC), which forms the core sphere patched with B-domains. Moreover, the S6 phase exhibits the widest stability window, while S5 has the narrowest one. The increased arms of A'-blocks in A2BCA3' architecture deflect the phase boundaries toward large fC and accordingly expand the regions of these patchy spheres due to the amplified effect of spontaneous curvature. In contrast, the increased arms of A-blocks in A3BCA2' remarkably expands the window of S6 but narrows those of the other patchy spheres, which is mainly caused by increased packing frustration resulting from the reduced extension of the more divided A-blocks. The widest window of the S6 phase reaches ΔfC ∼ 0.13, which is readily accessed by experiment. Our work not only demonstrates a self-assembly strategy to engineer the patchy spheres, but also sheds light on the regulation mechanism of the patchy number.

Immobilization of strontium in geopolymers activated by different concentrations of sodium silicate solutions.

Sodium silicate is always used as an activator for the synthesis of geopolymer. However, the effect of sodium silicate concentration on the geopolymer used as adsorbent was still unclear. Therefore, the immobilization of Sr2+ in geopolymers activated by different concentrations of sodium silicate was studied through kinetic and isotherm modeling and solid characterizations including XRD, FTIR, TG, SEM-EDS, and N2 adsorption-desorption isotherm. The adsorption amount of Sr2+ decreased with the sequence of S1, S2, and S3. According to the kinetic and isotherm modeling results, these sorption processes fitted better with pseudo-second-order, mainly governed by film diffusion. However, the diffusion mode was gradually closed to particle diffusion as for the sequence of S3, S2, and S1. Besides, the Langmuir model can be more befitting to sorption data than the Freundlich model, and the free energies decreased with the order of S1, S2, and S3. In addition, the specific surface areas did not change regularly with S1, S2, and S3. Thus, the distribution of Al tetrahedrons has a decisive role in the sorption process of Sr2+, even though the specific surface area is also a critical factor. More Al tetrahedrons can be formed under the activation of sodium silicate with higher concentration, leading to the low Si/Al molar ratio of the as-synthesized geopolymer.

SARS-CoV-2 spike and its adaptable furin cleavage site

SARS-CoV-2 spike and its adaptable furin cleavage site

CABE-RY: A PAM-flexible dual-mutation base editor for reliable modeling of multi-nucleotide variants

Multi-nucleotide variants (MNVs) represent an important type of genetic variation and have biological and clinical significance. To simulate MNVs, we designed four dual-mutation base editors combining hA3A(Y130F), TadA8e(V106W), and protospacer adjacent motif (PAM)-flexible SpRY and selected cytosine and adenine base editor-SpRY (CABE-RY), which had the best editing performance, for further study. Characterization and comparison showed that CABE-RY had a smaller DNA editing window and lower RNA off-target edits than the corresponding single base editors. Thus, we have established a versatile tool to efficiently simulate MNVs over the genome, which could be very useful for functional studies on MNVs in humans.

Moving Target Localization in Sports Image Sequence Based on Optimized Particle Filter Hybrid Tracking Algorithm

This paper proposes a fusion stadium positioning algorithm, which uses self-optimizing particle filter to integrate the improved athlete dead reckoning and WiFi position fingerprint algorithm for stadium positioning. In order to determine the initial absolute position of the stadium positioning, for athletes entering the stadium from the outside, a seamless switching algorithm outside the stadium is proposed, using the characteristics of high-altitude satellite GPS to find a suitable switching point as the initial absolute position. If in the stadium, WiFi static positioning determines the initial absolute position. Then, aiming at the problem that the poorly diversified particles cannot be better integrated and localized, a self-optimized particle filter algorithm is proposed. After resampling and retaining high-weight particles, the characteristics of low-weight particles are embedded in the copied high-weight particles. This can improve diversity, and we finally carry out fusion positioning. The target tracking algorithm based on Mean Shift has a fixed-scale tracking window, and the tracking effect of variable-size targets is not ideal. In this paper, an affine transformation algorithm is introduced to improve it. First, we iterate the adjacent image frames in reverse Mean Shift to determine the center position of the target and then use the corner matching method to perform template matching on the target to adjust the size of the tracking window. Through simulation verification, it is proved that the optimized particle filter hybrid tracking algorithm can achieve the ideal result when the target size changes. For the image sequence S1, the tracking window of the 20th frame and the 40th frame has a small offset, but the optimal position can be quickly found by Mean Shift iteration. For the image sequence S2, between the 40th frame and the 60th frame, the target occlusion causes the accuracy of the target template to decrease, and the Bhattacharyya coefficient is at a relatively low value. For the image sequence S3, the tracking effect of the optimized particle filter hybrid tracking algorithm meets the requirements.

Sequence stratigraphy, paleogeography, and coal accumulation in a lowland alluvial plain, coastal plain, and shallow-marine setting: Upper Carboniferous–Permian of the Anyang–Hebi coalfield, Henan Province, North China

To study the stratal stacking patterns and controls on coal accumulation in a lowland alluvial plain, coastal plain, and shallow-marine setting, the depositional facies, sequence stratigraphy, and paleogeography of the Carboniferous–Permian strata in the Anyang-Hebi (Anhe) coalfield, Henan Province, northern China were analyzed based on borehole cores. Ten facies were grouped into four facies associations, which represent alluvial plain, delta plain, delta front and prodelta, and offshore marine depositional environments. Ten sequences (S1 to S10, in ascending order) were defined on the basis of vertical facies stacking patterns. Sequences S1–S3, of Moscovian to early Asselian age, are composed mainly of offshore marine carbonate and mudstone, prodelta and delta front mudstone and sandstone, and delta plain sandstone, mudstone and coal. Sequences S4–S9, late Asselian to Wuchiapingian in age, consist of delta plain sandstone, mudstone and coal. Sequence S10, corresponding to the Changhsingian stage, is composed mainly of alluvial plain sandstone and mudstone. From S1 to S10, the Anhe coalfield experienced an environmental evolution from offshore marine, prodelta and delta front through delta plain to alluvial plain environments, an evolution from a low-accommodation to a high-accommodation setting, and a paleoclimatic change from humid to semi-arid conditions. Coal accumulation was controlled by a combination of relative sea-level fluctuation, paleoclimate and paleogeography. The cyclothemic nature of S2, including presence of parasequences, and the significant sea-level fall interpreted at the base of S4 may be related to Gondwanan ice dynamics in the late Carboniferous–early Permian. The thick coals are concentrated in the transgressive systems tracts of S2 and S4. The results of this study provide new insights into the environmental evolution of Carboniferous–Permian lowland alluvial plain, coastal plain and shallow-marine successions of the northern subtropical paleolatitudes and the controls on coal deposits in North China.

Image segmentation encryption algorithm with chaotic sequence generation participated by cipher and multi-feedback loops

The existing chaotic image encryption algorithms have common defects: (i) ciphertext does not participate in the generation processes of chaotic pseudo-random sequences and key sequences; (ii) the entire encryption process does not have a closed-loop structure. In order to solve above problems, in this paper, an image segmentation encryption algorithm based on hyperchaotic system is proposed. We decompose the scrambled sequence into three sequences of different lengths: S1, S2 and S3. Then, the initial values of the chaotic system are updated by the sequences S2 and S3 and using the updated initial value iterates the chaotic system and generates the key sequence K3, and the sequence S1 is encrypted by the sequence K3 to obtain the cipher sequence C1, using the sequences C1 and S3 updates the initial value of the chaotic system, and using the updated initial value iterates the chaotic system and generates the key sequence K4, and using the sequence K4 encrypts the sequence S2 to obtain the cipher sequence C2. Thus, ciphertext participates in the generation processes of chaotic pseudo-random sequences and key sequences, and the entire encryption process has a closed-loop structure. The experimental results show that the encryption algorithm has high security and sensitivity.

Pre-glacial and post-glacial history of the Scandinavian Ice Sheet in NW Russia – Evidence from Lake Ladoga

Lacustrine sedimentary records are valuable archives of regional paleoenvironmental and paleoclimatic changes. High-resolution seismic reflection data collected in 2013, in conjunction with data from sediment core Co1309, provide a detailed reconstruction of the preglacial and postglacial environmental and sedimentological history of Lake Ladoga (NW Russia) during the late Quaternary. The exceptionally good quality of the seismic data reveals an up to 90-m-thick sedimentary infill in undisturbed places, containing four distinct sequences documented in unprecedented detail. The deepest sequence S1 most likely represents the bedrock of the lacustrine basin, which was reshaped during the subsequent glaciations. The overlying sequence S2 contains large-scale mounds of variable dimension, shape and internal architecture, which suggest a complex history of formation and deformation. We interpret these mounds as a moraine-mound complex resulting from thrusting and bulldozing processes of pre-existing, subglacial sediments during the overall retreat of the Scandinavian Ice Sheet following the Last Glacial Maximum. The finely-layered sequence S3 coincides with varved sediments in core Co1309 that mark the Scandinavian Ice Sheet deglaciation in the Ladoga basin. The youngest sequence S4, characterised by a semi-transparent facies in the acoustic data, coincides with lacustrine sediments in core Co1309 that were deposited during the Holocene. Our results also document major thoroughgoing fault systems in the northwestern part of the lake basin, along with unconformities related to lake-level fluctuations, notably in the eastern-, western- and southernmost regions. By coupling hydroacoustic and sedimentological data, we are able to pinpoint lateral variations and modifications of sedimentary inputs through time during the evolution of the Ladoga basin, along with unravelling the paleoenvironmental history of Lake Ladoga since the pre-LGM.

Studies on factors affecting unconfined compressive strength of industrial rubber sludge containing heavy metals treated using ordinary Portland cement via stabilization/solidification technique

High concentration of selected heavy metals within industrial rubber sludge collected from rubber industry wastewater treatment plant has classified the waste as scheduled waste. Special treatment to the waste by using ordinary Portland cement via solidification/stabilization (S/S) technique has been performed in laboratory scale. The objective of this research is to determine related factors that affect unconfined compressive strength (UCS) performance of stabilised/solidified (s/s) cube specimens which contains industrial rubber sludge waste. Other parameters observed include the curing condition (i.e. air and water immersion curing method), waste composition, specimen age and density. The prepared fresh mix were cast in plastic moulds in order to produce 50 mm3 cubical shape specimens and leaved to set approximately 24 to 48 hours. The prepared specimen batches are S1 (90% OPC + 10% waste), S2 (70% OPC + 30% waste), S3 (50% OPC + 50% waste). UCS was performed on respective specimen age of 7 and 28 days. Positive results were obtained as relatively the average compressive strength of 7 day air cured specimens reach 5.25 MPa, 5.28 MPa, and 2.16 MPa for S1, S2 and S3.While, 28 days air cured specimens results are 9.59 MPa, 8.01 MPa, and 1.46 MPa for S1, S2, and S3 respectively. As for water immersion, the compressive strengths are 8.19 MPa, 4.93 MPa, and 1.90 MPa for 7 days, and 7.75 MPa, 10.10 MPa, and 2.11 MPa for 28 days at respective S1, S2 and S3 sequence. As conclusion, the specimens prepared passed the minimum requirement for secured landfill disposal which is at 1 MPa.

Seismic stratigraphic analysis for hydrocarbon exploration in the Beta Field, Coastal Swamp Depobelt, Niger Delta

Hydrocarbon exploration analysis from a seismic stratigraphic approach was carried out within the Beta Field, Coastal Swamp Depobelt, Niger Delta, to identify system tracts and sequence boundaries that could be associated with potential hydrocarbon accumulation. 3D seismic volume and data from four wells were analyzed. Depositional sequences, system tracts, sequence boundaries, and candidate maximum flooding surfaces were picked from logs, while reflection patterns and terminations were interpreted from seismic sections. Log shapes from gamma rays in combination with seismic facies analysis were interpreted in order to delineate lithology, depositional environments and depositional sequences. Seismic attributes were extracted and were draped on gridded surfaces from the interpreted seismic horizons. These were integrated with structure maps to obtain structural and stratigraphic trends, and the possible presence of reservoir sand. Five depositional sequences and nine seismic facies were identified within the field. The depositional sequences were designated Sequences S1 to S5 based on the depth of the occurrences and stacking patterns. This study reveals a progression from fluvial depositional settings to the shelf. The main reservoirs identified are the sand units of the highstand and lowstand within three depositional sequences (S1, S4 and S5) although interbedded sands within the TST of S4 and S5 are also suspected of being potential reservoirs. The channel sand deposits within the study area are suspected to be hydrocarbon bearing as they occurred within the complex fault trapping system popular in the Niger Delta. The application of seismic stratigraphy, as shown in this study, serves to encourage exploration in the Niger Delta where it could be effectively employed for reducing risk in hydrocarbon exploration.

Identification and complete-genome phylogenetic analysis of an epizootic hemorrhagic disease virus serotype 7 strain isolated in China

An epizootic hemorrhagic disease virus (EHDV) strain designated YN09-04 was isolated from sentinel cattle in China. The length of its complete genome was 19,344bp in total, consisting of 10 segments ranging in size from 810bp (S10) to 3942bp (S1). Based on phylogenetic analysis of the S2 sequence, YN09-04 clusters with EHDV serotype 7 (EHDV-7) strains form a distinct, well-supported subgroup, indicating that YN09-04 belongs to EHDV-7. However, the origin of the YN09-04 genome is very complex. The S2 and S6 of YN09-04 cluster with those of Japanese EHDV-7 strains, whereas the S1, S3, S4, S5 and S7 of YN09-04 share high nucleotide sequence identity and a close relationship with those of Japanese Ibaraki viruses, and the S8, S9 and S10 nucleotide sequences of YN09-04 are more similar to those of some Australian EHDV strains than to those of other isolates. These results suggest that the genome of YN09-04 likely originated from a reassortment event between EHDV strains that were similar to the current Japanese and Australian strains and that YN09-04 and some EHDVs from Japan and Australia share the same ancestors. This is the first report of the isolation, identification and complete-genome phylogenetic analysis of an EHDV-7 strain from China.