Sone Research Articles

Incorporation of topotecan into sesame oil - Nanoemulsion potentiates its cytotoxic effect in cancer cells

PurposeTo incorporate the topoisomerase I inhibitor, topotecan (TOPO), into oil-in-water nanoemulsion delivery system based on sesame oil (SO-NE), with the aim to enhance its anti-proliferative effect on MCF-7 breast, HCT116 colon, HeLa cervical, and HepG2 liver cancer cells. MethodsSO-NE was formulated by mixing different volume fractions of SO (3%), Tween 80 (10%), Span 20 (5%), and water (82%) using the high-pressure ultrasonication technique. SO-NE and TOPO-loaded SO-NE (TOPO–SO–NE) were physically characterized by zeta sizer and transmission electron microscope. The in vitro drug release of TOPO–SO–NE relative to the free TOPO solution was assessed by the dialysis bag technique. The cytotoxicity of the TOPO–SO–NE formula was determined in vitro using crystal violet assay. DAPI fluorescent stains were used for cellular morphology and death assessments. Additionally, TOPO cellular uptake and inflammatory effect after TOPO loading into SO-NE formula were determined. ResultsSO-NE formula was found to have negatively charged nanosized particles even after TOPO loading (Z-diameter = 73.66 ± 5.5 nm, Zetapotential = −7.63 ± 0.24 mV). In addition, TOPO–SO–NE formulation exhibited a beneficial TOPO prolonged and sustained release over 24 h. In all tested cell lines, TOPO–SO–NE formula exhibited considerable anti-proliferation effect with decreased IC50 and caused distinct apoptotic changes in cancer cells, which was evidenced by the enhanced cellular uptake of TOPO. The concentrations of interlukin-6 (IL-6), an inflammatory marker, in MCF-7, HCT116 and HepG2 cells were suppressed by TOPO–SO–NE treatment, but they got reversed in HeLa cells. ConclusionIncorporation of TOPO into SO-NE has remarkably improved its cytotoxicity in MCF-7, HCT116, HeLa and HepG2 cancer cells. The mechanisms of cell death were through the induction of apoptosis, enhancement of TOPO cellular accumulation ratios and affecting the inflammatory agent, interlukin-6 (IL-6), concentrations.

Influence of different cross-section on cyclic fatigue resistance of two nickel–titanium rotary instruments with same heat treatment: An in vitro study

Introduction: The aim of this study was to investigate the role of the cross-sectional design surface in improving the cyclic fatigue resistance by comparing two nickel–titanium (NiTi) rotary instruments with the same heat-treatment and grinding procedure. Materials and Methods: A total of 40 new NiTi instruments (20 S-One 25.06 taper and 20 AF Blue S4 25.06 taper) were tested in the present study. Both instruments were rotated at 300 rpm and with a torque setting of 2 Ncm) using an endodontic motor in the same artificial canal (90° angle of curvature and 3 mm radius). A Student's t-test was performed to determinate the differences in terms of time to fracture (TtF) and the length of the fractured portion between the two different instruments. Significance was set at P Results: The differences in terms of TtF for cyclic fatigue resistance between the two files were statistically significant (P 0.05). Scanning electron microscopy images showed patterns of cyclic fatigue striations. Conclusion: The results of this study confirm and enhance the role of cross-section in increasing the cyclic fatigue resistance of NiTi rotary instruments.

Environmental Flow Requirements of River Sone: Impacts of Low Discharge on Fisheries

Environmental flow of the river Sone at Indrapuri barrage was estimated using 36 years discharge data and the Global Environmental Flow Calculator Software. To maintain the river in moderate condition and to keep basic ecosystem functions intact, at least 18.9% of mean annual runoff (MAR) has been estimated, while the actual discharge of the river was merely 5.16% of MAR. The river presently holds 89 fish species, but 20 species reported in an earlier study were not observed, while 14 new fish species were encountered. Sediments, water and macro-benthic biota of the river were also studied to know the effect of low discharge.

The clinical observation of fluticasone salmeterol inhaler combined with salbutamol inhalation of oxygen-driven treatment of chronic obstructive pulmonary disease with acute exacerbation

Objective To observe the clinical efficacy of fluticasone salmeterol inhaler combined with salbutamol inhalation of oxygen-driven treatment of chronic obstructive pulmonary disease with acute exacerbation. Methods 60 patients of chronic obstructive pulmonary disease with acute exacerbation ,randomly divided into observation and control groups of 30 patients each. The control group on the basis of conventional treatment with salmeterol plus flutica sone inhalation powder compound and the oxygen-driven albuterol inhalation aerosol liquid, the other group by conventional therapy based on the use of oxygen-driven albuterol inhalation aerosol solution, then compare the clinical curative effects. Results There were 18 examples excellencely, i0 examples improve, 2 examples ineffective of observation group with total effective rate 93.3% ;9 examples excellencely, 11 examples improve, 10 examples ineffective of control group with total effective rate 66.7%. The contrast of total effective power with the two groups exists the staffstical significance （ X2 = 4.60,P 〈 0.05 ） o In the observation group, FEV1 and FVC of post-treatment, were better than pretherapy and post-treatment of control group （ t = 2. 629,2. 649,2.064,2.051, P 〈 0. 05 ）. Conclusion Salmeterol and fluticasone propionate inhalation powder hybrid atomizing inhalation of salbutamol effective than salbutamol alone. Key words: Pulmonary disease, chronic obstructive ; Administration, inhalation; Salmeterol, fluticasone ; Salbutamol

Study on treatment of dopamine extravasation-induced injury by lidocaine combining dexametha sone

Objective To discuss the treatment of dopamine extravasation-induced injury by lidocaine combining dexamethasone. Methods Animal models of dopamine extravasation-induced injury were made using 24 limbs of 6 white mice. These models were divided into 4 groups and given different treatments. Group A was treated with lidocaine combining dexamethasone, group B with lidocaine, group C with normal saline and group D was set as control. The treatment effect was observed. Results The disappearance time of edema in group A was the shortest among the 4 groups and was significantly shorter than that of group C,P＜0.05.Moreover, the pathological changes degree of local tissues in group A were also obviously lower than those of group B,C and D. Conclusion Measures must be taken timely to cope with dopamine extravasation-induced injury. Treatment with lidocaine combining dexamethasone proved to be a prompt, simple and safe method. Key words: Lidocaine; Dexamethasone; Dopamine; Drug extravasation; Animal

SELECTION PRINCIPLES RELATED TO αi-PROPERTIES

We\, investigate\, selection\, principles\, which\, are\, motivated\, by Arhangel'ski${\rm\check i}$'s $\alpha_i$-properties, $i=1,2,3,4$, and their relations with classical selection principles. It will be shown that they are closely related to the selection principle $\sone$ and often are equivalent to it.

Book Review: Inorganic Thermochromism. By K. Sone and Y. Fukuda

Book Review: Inorganic Thermochromism. By K. Sone and Y. Fukuda

Chaucer's Chimera: His Protosurrealist Portrait of Fame

1360 al on hye, above a dees, Sitte in a see imperiall, That mad was of a rubee all, Which that a carbuncle ys ycalled, Y saugh, perpetually ystalled, 1365 A femynyne creature, That never formed by Nature Nas such another thing yseye. For alther-first, soth for to seve, Me thoughte that she was so lyte 1370 That the lengthe of a cubite Was lengere than she semed be. But thus sone, in a whyle, she Hir tho so wonderliche streighte That with her fet she erthe reighte, 1375 And with hir hed she touched hevene, Ther as shynen sterres sevene. And therto eke, as to my wit, I saugh a gretter wonder yit, Upon her eyen to beholde; 1380 But certeyn y hem never tolde. For as feele eyen hadde she As fetheres upon foules be, Or weren on the bestes foure That Goddis trone gunne honoure, 1385 As John writ in th'Apocalips. Hir heer, that oundy was and crips, As burned gold hyt shoon to see; And, soth to tellen, also she Had also fele upstondyng eres 1390 And tonges, as on bestes heres; And on hir fet woxen saugh y Partriches wynges' redely. But, Lord! the perry arnd the richesse I saugh sittyng on this godesse!