Grazed grass is considered the cheapest feed available for dairy cows in temperate regions, and to maximise profits, dairy farmers must utilise this high-quality feed where possible. Recent research has reported that including white clover (Trifolium repens L.) in grass swards can have a positive effect on milk production. The aim of the present study was to quantify the effect of tetraploid and diploid perennial ryegrass (Lolium perenne L.; PRG) swards sown with and without white clover on the milk production of grazing dairy cows. Four grazing treatments were used for the study; tetraploid-only PRG swards, diploid-only PRG swards, tetraploid PRG with white clover swards and diploid PRG with white clover swards. Thirty cows were assigned to each treatment and swards were rotationally grazed at a stocking rate of 2.75 cows/ha and a nitrogen-fertiliser application rate of 250 kg/ha annually. There was no significant effect of ploidy on milk production. Over the present 4-year study, cows grazing the PRG–white clover treatments had greater milk yields (+597 kg/cow.year) and milk-solid yield (+48 kg/cow.year) than cows grazing the PRG-only treatments. This significant increase in milk production suggests that the inclusion of white clover in grazing systems can be effectively used to increase milk production of grazing dairy cows.