Robinia (R. pseudoacacia) is distinguished by a high level of ecological plasticity and is able to grow in different agroforestry areas under conditions of chestnut soils in southern Russia. The research was carried out in cluster collection sites of the Federal Research Center of Agroecology of the Russian Academy of Sciences (Lower Volga Station) and on the basis of the Volgograd Forestry State Educational Institution according to generally accepted methods in 2022. The purpose of the research was to analyze the technological features of the dynamics of growth and development of seedlings of R. pseudoacacia, to conduct a bioinformatic analysis of the gene sequence and to assess the prospects of their use for the production of planting material. Physiological indicators for assessing the Robinia generic complex stability have been identified. The economically valuable signs identified were morphological characteristics to the method of the Federal Scientific Center of Agroecology of the Russian Academy of Sciences, features of nitrogen fixation and the pigment complex. All the studied seedlings of R. pseudoacacia were at a sufficient level of nitrogen nutrition. On the territory of the Lower Volga station, plants formed a larger growth and biomass with the largest number of nodules than Robinia seedlings on the territory of the Volgograd Forestry State Budgetary Institution. However, the mass of nodules on the roots decreased with an increase in the humus content of the experimental site in Kamyshin (0.93%), which was explained by favourable weather conditions for the development of this plant during the growing season of seedlings and a sufficient amount of nutrients for the formation of these nodules – nitrogen and its compounds. This plantation planting material Robinia pseudoacacia (L.) had the property of adapting to the type of soil, thereby being a potential breed for growing in protective plantings and landscaping plantings of various functional purposes on chestnut soils of Russian Federation.