This study analyzed the tolerability of factors of flight work by Vietnamese pilots. The effects of aerobatic overloads (statoergometry), hypoxia (hypoxic test), and statokinetic loads (Otolith reaction-10 test) were simulated. To assess the functional state, computer stabilography and psychophysiological techniques were used to determine the reaction of choice and the critical frequency of light flashes. Height, bodyweight, blood pressure, heart rate, and blood oxygen saturation were recorded. In this study, 17 Vietnamese (group 1) and 24 Russian (group 2) pilots aged 1922 years were examined. Significant differences in height and bodyweight were found between the groups, which affected the tolerance of static muscle loads. Only 12% of the Vietnamese pilots completed the statoergometric test, and only one of the Russian ones did not reach the fifth stage. The conducted tests did not significantly affect the psychophysiological indicators, although they had multidirectional changes. Thus, when performing a normobaric hypoxic test, the blood pressure, heart rate, and blood oxygen saturation at the 5th minute of the recovery period returned to their original values. A slight increase in the pulse rate did not reach statistical significance. In general, the tolerance for normobaric hypoxia in both groups was good. However, a significantly smaller area of displacement of the pressure center of the statokinesiogram indicated a greater strain of adaptation mechanisms in group 1 than in group 2. The reaction to the statokinetic effect also indicated a satisfactory tolerance of the vestibular test Otolith reaction-10. Moreover, the tension of adaptive mechanisms in group 1, in contrast to group 2, was more pronounced. In addition, changes in the indicators of computer stabilography suggest a greater strain of adaptive mechanisms in Vietnamese pilots than in Russian pilots. The results indicate the need to develop methods of preparation for flights with aerobatic overloads and a system of psychophysiological training of pilots of the Socialist Republic of Vietnam to increase resistance to the influence of professional factors of flight work and the successful operation of Russian aviation equipment.