Russet Burbank Potatoes Research Articles

Effect of rate and time of fertilization on nitrogen and yield of Russet Burbank potatoes under center pivot irrigation

Applying less N on Russet Burbank potatoes (Solanum tuberosum L.) at planting time may reduce the potential loss of N from sandy soil by wind erosion and leaching early in the season. The objective of this study was to evaluate the effect of rate and time of N fertilization on potato production. Potatoes were grown in outdoor pot cultures with N rates of 75,150 and 300 ppm and in field plots with N rates from 112 to 448 kg N/ha applied in single and split applications. The results from pot cultures harvested after one month’s growth showed that yield of tops increased and tuber yield decreased as applied N at planting increased from 75 ppm to 150 or 300 ppm. Field results showed that the yield of potatoes was as high or higher when N applications were split between planting and when plants were 15 to 20 cm tall (early tuberization) as with the same amount of N applied at planting. Generally 112 kg N/ha at planting time was sufficient when additional N was applied after emergence. Split application of N resulted in more second growth on tubers than when all of the N was applied at planting.

Isolation and characterization from potato tubers of two polypeptide inhibitors of serine proteinases

Two polypeptides, isolated to electrophoretic homogeneity from Russet Burbank potato tubers, are powerful inhibitors of pancreatic serine proteinases. One of the inhibitors, called polypeptide trypsin inhibitor, PTI, has a molecular weight of 5100, and inhibits bovine trypsin. The inhibitor is devoid of methionine, histidine, and tryptophan and contains eight half-cystine residues as four disulfide bridges. The second inhibitor, polypeptide chymotrypsin inhibitor II, PCI-II, has a molecular weight of 5700 and powerfully inhibits chymotrypsin. This inhibitor is also devoid of methionine and tryptophan but it contains only six of half-cystines as three disulflde bonds. Both polypeptides strongly inhibit pancreatic elastase. In immunological double diffusion assays, polypeptide trypsin inhibitor and polypeptide chymotrypsin inhibitor II exhibit a high degree of immunological identity (a) with each other, (b) with a polypeptide chymotrypsin inhibitor (PCI-I, M r 5400) previously isolated from potato tubers, and (c) with inhibitor II, a larger (monomer M r ~ 12,000) inhibitor of both trypsin and chymotrypsin which has also been previously isolated from potato tubers. The four polypeptide proteinase inhibitors now isolated from Russet Burbank potato tubers cumulatively inhibit all five major intestinal digestive endo- and exoproteinases of animals. The inhibitors are thought to be antinutrients that are present as part of the natural chemical defense mechanisms of potato tubers against attacking pests.

Soil Treatments of Experimental Insecticides for Control of Aphids and Colorado Potato Beetles on Potatoes, 1981

Abstract The soil type for this experiment was a Ritzville silt loam, Mesic Calciorthidic HaploxerolIs, with a pH of 6.2 and organic matter of 1.6%, at Yakima, Washington. The soil was prepared for planting the week of Apr 13-17. Eptam herbicide was applied over the entire experimental area. The plots were treated and planted with Russet Burbank potatoes on Apr 23. The the preplant broadcast (PPBC) treatments the insecticide granules were applied with a cyclone seeder applicator and then the granules were worked into the top 6 inches of soil with a rototiller. For the planting time furrow (PTF) treatments the insecticide granules were dribbled through a hand-held, funnel-type applicator into the open furrow over the potato seed pieces and then the seed and granules were covered with soil. Each treatment was replicated 4 times in a randomized complete block design. Each plot was 2 rows (34 inches) wide and 10 ft long (1.72 m wide and 5.78 m long).

Production of Botulinum Toxin in Inoculated Pack Studies of Foil-Wrapped Baked Potatoes

Idaho Russet Burbank potatoes were surface or stab inoculated with 10 to 105 spores of Clostridium botulinum type A strain, overwrapped in aluminum foil, baked at 204 C for 50 min or 96 C for 3 h and then held at 22 or 30 C. The shortest incubations resulting in the first botulinogenic potatoes were inversely related to spore doses and ranged from 3 to 7 days; potatoes inoculated with 10 spores were toxic after 5 to 7 days. Total toxin in individual potatoes incubated 3 to 5 days were 5 × 103 to 5 × 105 mouse mean lethal doses. Toxin was not found at distances greater than 1.6 cm from the spore inoculation site. Results indicate that left-over, foil-wrapped, baked potatoes are a perishable food that must be refrigerated.

Double-dip caustic peeling of potatoes I: Laboratory-scale development

A modified caustic peeling process for potatoes was developed and evaluated. The process, called the double-dip process, comprises two separate caustic applications with an intervening holding period. Results on Russet Burbank potatoes showed that tissue removal was enhanced for the double-dip process when compared to the standard single dip process and that the double-dip process can effect reduction in caustic usage without requiring additional heat input.

Yield and quality of Russet Burbank potato as influenced by interactions ofRhizoctonia, maleic hydrazide, and PCNB

Soil treatment with pentachloronitrobenzene (PCNB) at 22.4 kg/ha a.i. reduced symptoms of theRhizoctonia disease (Rhizoctonia solani Kuhn) on potato (Solarium tuberosum L.) and resulted in significant increases in the numbers of stems and stolons. With disease control there was no effect on either total or U.S. #1 yield, but decreases of tuber size were evident and effects were observed on grade. Yield of malformed tubers was reduced by 35%, but this benefit was counteracted by a 41% increase of undersized tubers. Among U. S. 1’s, yield of non-size A’s (113 to 170 g) were increased by 22%, while tubers in optimum size ranges for french fries and bakers (> 284 g) were decreased by 24%. No effect was observed on carton sized tubers (170 to 369 g). Foliar sprays of maleic hydrazide (MH) applied on either of several dates (23 July, 2 Aug., 13 Aug.) at 3.4 kg/ha a.i. reduced total yield by 4.9 to 5.7%, but yields of U.S. #1 potatoes or sizes > 170 g were unaffected. Treatments with MH on either 23 July or 2 Aug. reduced yield of undersized potatoes by 23%. MH treatments did not interact with PCNB treatment to change either total yield or size, but adverse effects on U.S. #1 yields were indicated. MH treatments applied on either 2 Aug. or 13 Aug. reduced U.S. #1 yields among PCNB treated plots by 7 to 10%. MH reduced length/width ratios and, depending on date of application, increased specific gravity. PCNB did not influence tuber shape or specific gravity, but a significant reduction of standard deviations among length/width ratios indicated greater uniformity of shape with treatment.

Specificity of the Carboxypeptidase Inhibitor from Potatoes

Carboxypeptidases from animal, plant, fungal, and bacterial sources were tested for their ability to bind to the carboxypeptidase inhibitor from Russet Burbank potatoes. Enzymes which participate in the degradation of dietary protein were partially purified from animal species as diverse as the cow and the limpet, and all were potently affected by the inhibitor. However, several zymogens of the enzymes in this group were tested and shown not to bind immobilized inhibitor. With the exception of an enzyme from mast cells and a novel carboxypeptidase A-like enzyme from bovine placenta, all animal carboxypeptidases which were not of digestive tract origin were not affected by the inhibitor. The inhibitor had no effect on the enzymic activities of all plant and most microbial carboxypeptidases. However, a strong association between the inhibitor and Streptomyces griseus carboxypeptidase has been noted previously and a low affinity (K(i) about 10 micromolar) for a carboxypeptidase G(1) from an acinetobacterium was found in this study.

Stability of Proteinase Inhibitors in Potato Tubers During Cooking

ABSTRACTThree proteinase inhibitors, Inhibitor I (a chymotrypsin inhibitor), Inhibitor II (a chymotrypsin and trypsin inhibitor), and Carboxypeptidase Inhibitor were quantified immunologically in extracts of various tissues of Russet Burbank potato tubers and were monitored over various intervals during boiling (30 min), oven baking (80 min, 191°C) and microwave baking (7 min) to assess the effectiveness of cooking in denaturing the potentially toxic proteins. In cooked tubers, Inhibitor II was completely inactivated during all cooking treatments and Inhibitor I was partially inactivated, depending upon the cooking method. The Carboxypeptidase Inhibitor was extraordinarily stable during all of the cooking conditions. Overall, microwave baking was most effective for cumulatively inactivating Inhibitor I and Inhibitor II. The unusual stability of CPI to all types of cooking raises questions concerning the effect of this inhibitor on the total digestibility of cooked potato proteins.

Ultrastructure of potato leaf phloem infected with potato leafroll virus

Electron microscopy of Russet Burbank potato ( Solanum tuberosum L.) leaf midveins infected with the potato leafroll virus (PLRV) revealed the pathological effects induced by the virus in the phloem. Virus particles were observed in mature sieve elements, companion cells, and plasmodesmata connecting the two kinds of cells. An early indication of virally induced cellular disturbance in companion cells was dilation of mitochondrial cristae followed closely by the appearance of vesicles in the parietal cytoplasm. One type of vesicle contained fibrils suggestive of nucleic acids and the second type carried an electron opaque material. Both types of vesicles were surrounded by two membranes. Rough endoplasmic reticulum (ER) formed the outer membrane. Both vesicle types fused with the nuclear envelope and were taken up into the nucleus. PLRV particles appeared mainly in the cytoplasm, where they were either scattered or localized along the membranes of mitochondria, chloroplasts, and vacuoles. In some cells, groups of particles were enclosed in the vacuole. The relation of virus particles to the nucleus remains problematic: there was no evidence that uptake of vesicles into the nucleus resulted in the formation of particles in this organelle. Cells in which particles formed became degenerated. Mitochondria swelled and cristae separated from the envelope and clumped. Most organelles including the nucleus, ribosomes, rough ER, and chloroplasts broke down leaving a fibrillar material interspersed with virus particles.

Multiplication ofPseudomonas solanacearum in resistant potato plants and the establishment of latent infections

When 1-mo-old plants of a wilt-resistant clone ofSolanum phureja (1386.15) were stem-inoculated with three strains ofPseudomonas solanacearum (K60, S123, and S206), the bacteria multiplied rapidly at the point of inoculation and then moved in the vascular system to other parts of the stem. Resistant plants showed a remarkable ability to support relatively high populations of the bacterium in the absence of disease symptoms. Although multiplication in this resistant clone was substantially less than in susceptible Russet Burbank potato plants, large numbers of bacteria (up to 624 × 104 cells of K60 per 5-cm stem segment) reached the base of the stem of plants maintained at high temperature (28°C) for 20 days after stem inoculation. From the base of the stem, the bacteria moved rapidly into the roots and tubers. Strains ofP. solanacearum differed in their ability to cause latent tuber infection in different resistant potato clones. When 11S.phureja ×S. tuberosum hybrids were stem-inoculated, maintained at 28°C for 3 wk and then grown to maturity at 20°C., most of the clones yielded tubers infected by one or more strains. The race 1 strain (K60) was the most infectious; 53.8% of all tubers harvested from all plants inoculated with this isolate carried latent infections. Because one clone (BR 53.1) never yielded infected tubers, there appear to be genetic factors which may be useful in breeding programs aimed at eliminating latent tuber infection.

CHEMISTRY OF BAKED POTATO FLOUR: FURTHER IDENTIFICATION OF HETEROCYCLIC COMPOUNDS IN THE VOLATILE FLAVOR OF BAKED POTATO

ABSTRACTThe gas chromatographic fractions of a baked potato flavor which was isolated from 540 lb of Idaho Russet Burbank potatoes were analyzed by infrared and mass spectrometry. A large number of heterocyclic compounds were identified. This paper reports the identification of 9 furans, 2 oxazoles, 3 thiophenes, 2 pyrroles, 2 pyridines, 1 dioxolane, and 1 trioxane. It was noted that heterocyclic compounds with formyl or acetyl substituents had aromas with nutty characteristics.

Influence of time of application of maleic hydrazide on the yield and quality of Russet Burbank potatoes

A three-year study in central Wisconsin with timed foliar applications of maleic hydrazide at 3 lb/A (3.36 kg/ha) resulted in an improvement in quality and a favorable alteration in the shape of tubers of the Russet Burbank potato variety. Application in early and mid-July resulted in an increase in yield of U.S. 1 tubers and reduced yield of malformed tubers. Over 40 percent fewer misshapen tubers were produced when applications of maleic hydrazide were made during July when compared with tubers from untreated plants. Applications made in midor late July resulted in tuber development with a more favorable length to diameter ratio of U. S. 1 tubers, again compared with tubers from untreated plants. Maleic hydrazide applied in early or mid-June resulted in severely reduced yield and increased incidence of misshapen tubers. When applied in July or early August, no effect on tuber specific gravity was found. Maleic hydrazide functioned as an effective sprout inhibitor on tubers from all treatment dates except when applied in early June.

Foliar Sprays of Synthetic Pyrethroids and Numbered Chemicals for Control of Colorado Potato Beetle, Test 10, 1978

Abstract 'Russet Burbank potatoes were planted on Mar 30 in Rirzville silt loam soil (Mesic Calciorthidic Haploxerolls) at Yakima, WA. A high infestation of the second generation of Colorado potato beetle (CPB) adult and larvae were defoliating the plants by Aug 7, the time of insecticide application. The insecticides were applied as foliar sprays in 40 gal/acre with a 2-row portable boom CO2 hand sprayer. The plots were 2-rows wide (5.6 ft) and 25-ft long and each treatment was replicated 4 times in a randomized complete block design. The number of live adults and larvae per rr/ of potato foliage per plot was counted on Aug 8 and 11 (1 and 4 days after treatment). Also, on Aug 8 the potato foliage between the 2 rows of each plot was separated and the number of dead and live adults and larvae per m* of soil surface plot was counted. Since both dead and live adults and larvae were on the soil and only live adults and larvae were on the plants, the percent mortalities were determined as follows: 1) percent mortality for adults or larvae on the soil based on number dead out of total number dead and live on soil; 2) percent mortality of adults or larvae based on number dead on soil out of total number on plants and soil; 3) percent mortality of CPB population based on number dead adults and larvae on soil out of total numbers of dead adults and larvae on plants and soil. The number of insects and the calculated percent mortality for each plot were subjected to analysis of variance using DMRT.

Influences of the Rhizoctonia disease on production of the Russet Burbank potato

Pentachloronitrobenzene (PCNB) treatment (20 lb/A – 22.4 kg/ha) showed significant reduction ofRhizoctonia (Rhizoctonia solani Kuhn) symptoms on potato. The PCNB treatment increased the number of non-girdled stolons as compared to untreated plots by 50, 34, and 30% at respective seedpiece spacings of 6, 9, and 12 in (15, 23, and 30 cm). Numbers of non-girdled stolons in PCNB-treated plots at 9- and 12-in spacing did not differ significantly from those in untreated plots at 6-in spacing.

Utilization by Chicks of Half-Cystine from Native and Denatured Proteinase Inhibitor Protein from Potatoes

SummaryIn a proteinase inhibitor protein fraction obtained from the soluble proteins of Russet Burbank potatoes, over 78% of the total half-cystine rich proteins are inhibitors of the pancreatic proteases, trypsin, chymotrypsin, and carboxypeptidase A. This protein fraction was utilized to supplement a half-cystine limited chick diet to determine the availability of half-cystine residues in native and autoclaved (denatured) inhibitor proteins. Chicks fed diets supplemented with native inhibitor protein showed a severe growth depression. Weight gain of chicks fed autoclaved inhibitor protein were equivalent to chicks whose diets were supplemented with half-cystine. The experiments indicated that high protein potato selections based on high inhibitor contents should provide nutritionally available half-cystine, provided the potatoes are cooked before consumption.

Reaction to phytotoxins in a potato population derived from mesophyll protoplasts

Alternaria solani, the causal agent of early blight disease in potato, produces two host-specific, lipidlike toxins in culture. Both compounds are required in the leaf bioassay for the elicitation of typical early blight symptoms, but the compounds are individually inactive. The procedures for the preparation of both compounds are outlined. These compounds can be used effectively to select for toxin-insensitive and sensitive clones of a Russet Burbank potato cultivar that have been regenerated from single mesophyll protoplasts. Furthermore, both sensitivity and insensitivity to the toxins in these clones is well correlated with susceptibility and resistance to A. solani. Potato clones that have been produced by somatic cell regeneration techniques maintain their reaction type to these fungal toxins for at least two generations of vegetative propagation. The genetic basis for this variation among these potato clones remains to be explained.

Lipid composition of potatoes as affected by storage and potassium fertilization

The effects of storage treatments and potassium fertilization on the phospholipid, triglyceride and free fatty acid content of Russet Burbank potatoes were determined. Thin-layer chromatography of the lipids indicated at least 18 types of lipids were present. Eight were phospholipids which were the predominant lipid class in the potatoes. Phosphatidylcholine, phosphatidylinositol, phosphatidylethanolamine and phosphatidic acid comprise the major phospholipids. A large increase in phosphatidic acid and a decrease in phosphatidylcholine were observed in potatoes stored at 5.6 C (42 F). Potatoes fertilized with 240 lbs of potassium (calculated as K2O) had a higher content of triglycerides compared to potatoes with no fertilization. Potatoes stored at 5.6 C (42 F) exhibited a decrease in triglyceride and phospholipid content but the free fatty acids were increased. The various lipid contents of reconditioned potatoes were similar to those of freshly harvested potatoes.

Factors influencing reconditioning of Russet Burbank potatoes

Studies were made to determine factors which influence reconditioning of Russet Burbank tubers. Tubers from plants grown under low fertility were more difficult to recondition than tubers from plants adequately fertilized. Low specific gravity potatoes were also more difficult to recondition than high specific gravity potatoes even though the initial sugar content was approximately equal. The stem portion of Russet Burbank tubers lost considerably less sugars than the bud portion during the reconditioning process. It appears that factors which are responsible for high sugar development are also responsible for difficulty in sugar loss. Results also indicate greater difficulty in reconditioning early in the storage period with the loss of sugars becoming easier as the storage season progressed into spring.

Influence of low fertility and vine killing on sugar development in apical and basal portions of Russet Burbank potatoes

Russet Burbank tubers from plants which died prematurely (low fertility plots) accumulated significantly greater amounts of reducing sugars in storage than tubers from plants which were adequately fertilized. Also, a significantly greater difference in relative amounts of reducing sugars between the apical and basal portions was obtained from tubers of the low fertilizer regime. The basal portion had a three times greater amount of sugars than the apical portion. This is of particular interest to processors of French fries who try to obtain uniformity of color in processed fries. The difference in total reducing sugar accumulation in storage between tubers of high and low fertilizer regimes was greater at the lower storage temperatures. Tubers allowed to mature in the ground (second harvest date) accumulated less sugars in storage than tubers dug shortly after vine kill.

Respiration rates and sugar accumulation in normal and moisture stressed Russet Burbank potatoes

Studies were made on Russet Burbank tubers grown under normal and moisture stressed conditions. Stressing the tubers caused increased reducing sugar accumulation in the basal end during storage. Respiration rates of apical and basal portions of stressed and normal tubers were followed during the growing season and in storage. Although its sugar content was greater, the basal end of stressed tubers respired at a significally slower rate than apical ends from the same tubers. Normal basal and apical portions showed no statistical differences in respiration although the apical end respired at a slightly faster rate than the basal end. A positive linear correlation between sugar concentration and respiration rate of the apical portion was obtained. Differences were found in the abilities of apical and basal ends to alter respiration rates in response to changes in sugar concentration. The resprationof the apical end was the more sensitive to increased sugar concentration. The basal end of stressed tubers lost some of its ability to respond, probably because of increased physiological aging caused by the stress conditions.