ABSTRACT A 15th century Tibetan biography offers a rare glimpse into the life of the Tibetan princess Chokyi Dronma (1422–1455), who experienced marriage, childbirth, the death of her daughter and eventually became a Buddhist nun and a famous spiritual master. Recognised as an emanation of the Buddhist deity Dorje Phagmo, she became the founding figure of Tibet’s most famous female reincarnation line. Written close to the events by one of her fellow monks and rich in daily life details, this account offers an ideal opportunity to explore gender and kinship practices among Tibetan rural elites of the time. It also gives a rare insight into the hurdles that women faced when they decided to challenge social and cultural conventions to pursue spiritual aims. An anthropologically informed study of this manuscript offers a unique opportunity to explore kinship terminology and practices as well as particular instances of kinship politics at a time in which marriage alliances were essential for the relationships among Tibet's regional polities. It also offers useful insights into kinship networks that underpinned patronage practices, highlighting the often mis-recognised importance of women (including their role in printing projects). Finally, it enables the exploration of Tibetan spiritual kinship and reincarnation contributing to wider debates in the anthropology of kinship and relatedness. Substances and spiritual principles appear to be symbolic fields connecting past and present – they constitute the backdrop for practical negotiation not only in Chokyi Dronma’s biography but also in many other historical sources and ethnographic cases.