Native vegetation restoration, such as pines, is an effective measure for mitigating soil erosion. However, the impact of pine needle litter on runoff, soil erosion, and hydrological responses on karst hillslopes remains unclear. This study investigated these factors during the rainy season from June to August 2021 under six distinct treatments (flat, gentle, and steep slopes with and without pine needle coverings). The results showed that pine needle litter significantly increased the soil water content, particularly on steeper slopes, by improving the water-holding capacity. It also substantially reduced runoff (by 23.8%) and soil erosion (by 92.5%). The critical rainfall threshold for generating runoff did not occur in the pine needle covering treatment, whereas it was 19.3 ± 8.8 mm in the bare soil treatment under wet conditions. These findings highlight the efficacy of pine needle cover in mitigating runoff and soil erosion in karst areas, suggesting it as a sustainable alternative against erosion, and underscoring the importance of pine plantations in degraded soil restoration efforts.