Ruminant Production Research Articles

Effects of bacteriocin-producing Lactiplantibacillus plantarum on bacterial community and fermentation profile of whole-plant corn silage and its in vitro ruminal fermentation, microbiota, and CH4 emissions

BackgroundSilage is widely used to formulate dairy cattle rations, and the utilization of antibiotics and methane emissions are 2 major problems for a sustainable and environmentally beneficial ruminant production systems. Bacteriocin has received considerable attention because of its potential as an alternative to antibiotics in animal husbandry. However, the impact of bacteriocin-producing lactic acid bacteria on the microbiological conversion process of whole-plant corn silage and rumen fermentation remains limited. The purpose of this study was to assess the effect of 2 class IIa bacteriocin-producing strains Lactiplantibacillus plantarum ATCC14917 and CICC24194 on bacterial community composition and ensiling profiles of whole-plant corn silage and its in vitro rumen fermentation, microbiota, and CH4 emissions.ResultsBoth bacteriocin-producing strains increased the lactic acid concentration in silage fermented for 7 d, whereas the lowest lactic acid was observed in the ATCC14917 inoculated silage fermented for 90 d (P < 0.05). The highest DM content was observed in the CICC24194 treatment (P < 0.05), and the silages treated with both strains had the lowest DM loss (P < 0.05). Bacteriocin-producing strains promoted the growth of Levilactobacillus brevis on d 60 of ensiling. In addition, treatment with bacteriocin-producing strains increased the in vitro DM digestibility (P < 0.05) and decreased the CH4 production (P < 0.05). The results of random forest and clustering analyses at the genus level showed that ATCC14917 increased the relative abundance of the influential variable Bacillus compared to that in the control group, whereas CICC24194 decreased the relative abundance of the influential variable Ruminococcaceae UCG-005. The CICC24194 treatment had the lowest total bacterial, fungal, protozoan, and methanogen populations (P < 0.05).ConclusionsBoth class IIa bacteriocin-producing L. plantarum strains improved the fermentation quality of whole-plant corn silage by regulating the bacterial community composition during ensiling, with CICC24194 being the most effective. Both bacteriocin-producing strains mitigated CH4 production and improved digestibility by modulating the interactions among rumen bacteria, protozoa, methanogens, and the composition of fibrolytic bacteria.

The role of monoammonium glycyrrhizinate as a methane inhibitor to limit the rumen methane emissions of Karakul sheep

Methane (CH4) from ruminant production systems produces greenhouse gases that contribute to global warming. Our goal was to determine whether monoammonium glycyrrhizinate could inhibit CH4 emissions over the long term without affecting animal performance and immune indices in Karakul sheep. This study aimed to assess the effects of medium−term (60 days) addition of monoammonium glycyrrhizinate on growth performance, apparent digestibility, CH4 emissions, methanogens, fibre-degrading bacteria and blood characteristics in Karakul sheep. Twelve male Karakul sheep (40.1 ± 3.59 kg) with fistula were randomly divided into two groups (n = 6): the Control group received a basal diet + the same volume of distilled water (30 ml) and the Treatment group received a basal diet + 8.75 g/kg monoammonium glycyrrhizinate injected via fistula. The adaptation stage was 15 days, and the measurement stage was 60 days. The sampling during the measurement stage was divided into two stages, stage I (1 ∼ 30 d) and stage II (31 ∼ 60 d). The results showed that monoammonium glycyrrhizinate significantly reduced the relative abundance of Bacteroides caccae, daily CH4 emission and protozoa population, significantly increased the relative abundance of Lachnospiraceae bacterium AD3010, Lachnospiraceae bacterium FE2018, Lachnospiraceae bacterium NK3A20, Lachnospiraceae bacterium NK4A179 and Lachnospiraceae bacterium V9D3004 in stage I (P < 0.05); significantly increased the relative abundance of Lachnospiraceae bacterium AD3010, but significantly decreased the relative abundance of Lachnospiraceae bacterium NK4A179 and Lachnospiraceae bacterium C6A11 in stage II (P < 0.05). Therefore, monoammonium glycyrrhizinate could be used as a CH4 inhibitor to limit the rumen CH4 emissions of Karakul sheep in short−term period (30 days) without affecting the growth performance, fibre digestibility and blood parameters.

Differential Age-Based Response Induced by a Commercial Probiotic Supplementation in Pastured Goats.

The potential benefit of probiotics in small ruminant production systems has largely been unexplored. We evaluated the effect of a goat commercial probiotic on health and performance indicators in pastured goats from birth until 10months. We randomly allocated 26 newborn nursing goat kids to two groups: a control group that received saline and a treatment group that received a commercial probiotic paste orally. We evaluated select observable health indicators (inappetence, diarrhea, coughing), weight, immunity (IgA, IgG, and innate immune response), total protein, hematocrit (HCT), total lactic acid bacteria (LAB), total coliforms, and prevalence of Escherichia coli (E. coli) primary virulence genes (stx1, stx2, and eae) during the experimental period. The results revealed no significant differences in the health indicators, LAB count, and total E. coli count. Prevalence of stx1 at 1week of age and both stx1 and stx2 genes 4months post-weaning was significantly (P < 0.05) higher in probiotic-supplemented goats. Probiotic supplementation significantly (P < 0.05) increased the total protein and IgA 1month post-supplementation during the pre-weaning period and innate immune markers 2days post-weaning. The HCT in probiotic-supplemented goats was significantly (P < 0.05) higher at 1 and 2months post-weaning. The growth rate was not affected by probiotic supplementation in pre- and peri-weaned goats but was significantly (P < 0.05) lowered in goats older than 4months in the supplemented group. In this pastured goat production study, there were mixed responses to a commercial probiotic in healthy goats based on age. The study suggests that early daily probiotic supplementation in pre-weaned pastured goats may have immune stimulation benefits, but in older healthy animals, post-weaning net benefits are unclear and further research is recommended.

Temperament and production in ruminants: the microbiome as one of the factors that affect temperament

The temperament of ruminants has been studied for several decades and has similarities with temperament in humans. Temperament refers to the response of an animal to a situation that the animal perceives as challenging. Temperament can affect most biological functions, primarily the stress axis but also other functions that can impact production traits and the health and welfare of the animal. An essential part of the expression of temperament is the way that an individual perceives a stressor. Studies using molecular tools have started to identify the central pathways that are involved in the expression of temperament. Recent studies have led to the proposal that the rumen microbiome could play a role in temperament, similar to the way that the gut microbiota can influence the brain in monogastric species. The nature of the interactions between the gut microbiome and the temperament of ruminants needs to be explored. In the future, manipulation of the rumen microbiota could modify the temperament of production animals and benefit their health and welfare.

Qualitative Production of Mixture Silage within a Sustainable Concept

Climate change and seasonality in forage production have caused alterations in animal feed. Thus, this study evaluated the composition of silages from soybean (Glycine max (L.) Merrill) mixed with tropical grasses. The experimental design was randomized blocks with four replications. Treatments were silage from soybeans, silage from soybeans with Aruana Guinea grass (Megathyrsus maximus cv. Aruana), and silage from soybeans with Congo grass (Urochloa ruziziensis cv. Comum). Silos were stored for 60 days in the laboratory at room temperature. The silage from soybeans with Aruana Guinea grass showed the highest contents of dry matter, crude fiber, neutral detergent fiber, insoluble nitrogen in neutral detergent fiber, and insoluble nitrogen in acid detergent fiber but the lowest levels of crude protein and ether extract. The highest content of mineral material and hemicellulose was observed in the silage from soybeans with Congo grass. The silages from soybeans and soybeans with Congo grass showed no significant differences for acid detergent fiber and lignin. In conclusion, the use of tropical grasses as a component to improve the quality of silage from soybeans is an alternative for forage conservation in ruminant production systems, especially at the dry season.

An exploration of biodiversity limits to grazing ruminant milk and meat production

The production and consumption of animal-source foods must be transformed to mitigate negative environmental outcomes, including greenhouse gas emissions and land-use change. However, livestock are also key for food production and for livelihoods in some settings, and they can help preserve biodiversity and certain ecosystems. Previous studies have not yet fully explored sustainability limits to the use of grazing lands for food production in the context of biodiversity. Here we explore ‘biodiversity limits’ to grassland ruminant production by estimating the meat and milk production from domestic ruminants limited to grazing areas and stocking densities where livestock can contribute to the preservation or restoration of biodiversity. With biodiversity-friendly grazing intensities at 0–20% biomass removal depending on aridity, this take on biodiversity limits corresponds to 9–13% and 26–40% of the current grassland-based milk and meat production, respectively. This equals only 2.2 kg of milk and 0.8 kg of meat per capita per year, globally, but altered management and moving from meat-specialized to meat-and-dairy systems could increase the potential production while still remaining within this approach to biodiversity limits.

Perspectives on progression of transboundary disease, one health and ecosystem health management in the Greater Mekong Subregion and beyond

Livestock production in the Greater Mekong Subregion (GMS) reflects the inefficient smallholder ‘keeper’ system that has been slow to adapt to the rapidly expanding demand for animal protein-sourced foods in the region as urban economies have flourished and food preferences altered. The prolonged surge in demand, with only modest increases in local production, has increased the movement of animals and products into and from the GMS, accompanied by surging risks of transboundary animal disease (TAD) incursions, including the one health (OH) threats of zoonoses and antimicrobial resistance. As a consequence, the region has been subjected to epidemics of highly pathogenic avian influenza (HPAI), new strains of foot-and-mouth disease (FMD) virus, with recent incursions of African swine fever (ASF), lumpy skin disease (LSD), porcine reproductive and respiratory syndrome (PRRS) and risks of peste petits ruminants (OPR) occurring in Southeast Asia (SEA) and beyond. These incidents reflect inadequate biosecurity, a sustainability issue that was clearly of relevance in the covid-19 pandemic, reflecting ecosystem health (EH) deficits, including land-use issues and unregulated trading in wildlife-sourced and poorly processed foods through the predominant ‘wet markets’. These challenges are increasingly confounded by slow adaption to the impacts of the climate crisis, including flooding, drought, crop failures and hypothermia episodes. The increase in animal and product movements enhances disease transmission risk, yet coincides with emerging concerns of greenhouse-gas emissions (GHGe) from livestock production, especially from large ruminants, as the world attempts to find pathways in managing the climate crisis. Despite the prolonged collaborative efforts of the SEA China FMD program from 1997 to 2023, a recent review confirmed persistent deficits in biosecurity, vaccine resourcing, disease surveillance, engagement of farmers, and national emergency disease-response capacities. A major project is about to fund major improvements in livestock value chains in Cambodia, including more effective biosecurity, surveillance and emergency disease-response capacities for TADs, antimicrobial resistance (AMR) and zoonoses. Similarly, a private-sector investment in Laos has developed a more climate-resilient livestock-feeding system that decreases GHGe impacts from ruminant production. These developments are likely to extend beyond both countries and be potentially transformational for the livelihoods of many of the poorest citizens in the region.

Molecular prevalence and distribution of tick-borne bacterial and protozoan pathogens of sheep and goats in Africa: A systematic review and meta-analysis

Tick-borne pathogens (TBPs) are a major impediment to the health, welfare, and production of small ruminants across the world, including Africa. Comprehensive information about the epidemiology of TBPs infecting sheep and goats across Africa is lacking. Therefore, this study was undertaken to determine the prevalence through a meta-analysis of selected TBPs in blood DNA from domestic sheep and goats in Africa obtained using molecular-based methods. The literature review was done according to Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines using five English electronic databases (PubMed, Science Direct, Springer Link, Web of Science, and African Journals Online (AJOL). The search was performed with no restriction in time through to 18th January 2023. Of the 63 full-text articles subjected to eligibility, only 30 articles met the eligibility criteria and were included in the review. The overall pooled prevalence of selected TBPs varied considerably between host species (sheep vs. goats), with Anaplasma ovis (44.50 vs. 48.40%), Ehrlichia ruminantium (5.50 vs. 2.00%), Coxiella burnetii (4.40 vs. 1.70%), Borrelia theileri in sheep (5.20%), Babesia ovis (1.70% vs. 1.90%), Theileria ovis (40.50% vs 10.00%), T. separata (1.00% vs 1.00%) and T. lestoquardi in sheep (8.40%). However, the prevalence of the selected TBPs was generally higher in sheep compared to goats. Several genetic loci were targeted in the characterization of tick-borne pathogens, such as 16S rDNA, groEL, and msp4 for Anaplasma ovis, pCS20 for Ehrlichia ruminantium, Insertion Sequence (IS1111) for Coxiella burnetii, flaB (flagellin) and 16S rRNA for Borrelia theileri, 5.8S rRNA and 18S rRNA for Babesia/Theileria, as well as the utilization of numerous PCR variants including conventional polymerase chain reaction (PCR), nested-PCR, qPCR, Loop-mediated isothermal amplification (LAMP), and reverse line blotting (RLB). In conclusion, A. ovis was the most widely distributed and prevalent TBP affecting small ruminants within the continent. Hence, this warrants adequate attention towards early diagnosis and treatment of infected animals as well as the control of the tick vectors involved in their transmission.

NUTRITIONAL VALUE AND IN VITRO DRY MATTER DEGRADABILITY IN MEXICAN SUNFLOWER: Tithonia diversifolia Helms (Gray)

&lt;p&gt;&lt;strong&gt;Background&lt;/strong&gt;: &lt;em&gt;Tithonia diversifolia&lt;/em&gt; Helms (Gray), is a robust shrubby forage plant with high integral biomass yield (stem and leaves); susceptible to heliophany and conditioning of bromatological characteristics, nutritional value, and modification of the rumen environment is due to genetic variability between genotypes. &lt;strong&gt;Objective:&lt;/strong&gt; To evaluate the macromolar quality of the biomass of introductions &lt;em&gt;T. diversifolia&lt;/em&gt; Helms (Gray) established in two locations, and the nutritional value, gas production and &lt;em&gt;in vitro&lt;/em&gt; rumen environment promoted by the whole plant biomass established in Ecuador. &lt;strong&gt;Methodology:&lt;/strong&gt; The chemical composition of the full plant (PITD) was considered based on Dry Matter (DM), Organic Matter (OM), Mineral composition (MC), Crude Protein (CP), neutral Detergent Fiber (NDF), Acid Detergent Fiber (ADF). I&lt;em&gt;n vitro&lt;/em&gt; gas production was evaluated according to Ankom Technology, considering Tiffon 85 there (&lt;em&gt;Cynodon dactylon&lt;/em&gt;) at control, quantified or ammoniacal nitrogen (N-NH&lt;sub&gt;3&lt;/sub&gt;), short-chain fatty acids (SCFA) and pH. The experiment was developed in a completely randomized design in factorial arrangement with three repetitions for the variable chemical composition, and the &lt;em&gt;in vitro&lt;/em&gt; digestibility of the date processed through a random complete block design with six repetitions, in the ruminal ambient and production SCFA was analyzed through a completely randomized block design with three repetitions. &lt;strong&gt;Results:&lt;/strong&gt; There were variable responses in the DM content for the effect of interaction P &amp;lt;0.01, and higher mineral content in El Carmen, Ecuador for introduction 13.5, with respect to the rest of the cultivars in both locations. NDF was significantly expressed (p&amp;lt;0.001) in variety 1.2 that grew in Candelaria, Ecuador. For the rest of the variables, no differences were recorded (P&amp;gt;0.05). The gas production of the introductions, except for 1.2, was similar to that produced by the control (Tifton 85), however, in the degradability of the OM, net gas production (Net GP) of DM and OM, stood out. introduction 1.2 compared to the control (P&amp;lt;0.01) and the rest of the introductions. The net GP of NDF exceeded 1.2 (p&amp;lt;0.02) than the control, also quantitatively to produce Acetic and Propionic Fatty Acid. &lt;strong&gt;Implications:&lt;/strong&gt; The introductions of &lt;em&gt;T. diversifolia&lt;/em&gt; Helms Gray based on the results obtained preserve macromolar characteristics, gas production and SCVFA production (short chain volatile fatty acids) in contrasting ecosystems such as Valle del Cauca, Colombia and Manabi in Ecuador. &lt;strong&gt;Conclusions:&lt;/strong&gt; The introductions of &lt;em&gt;T. diversifolia&lt;/em&gt; Helms Gray, maintain a good protein composition, produce low methane contents, have high performance in the fermentation of organic matter and the cell wall, which suggests its future use as a complement to the ration in conditions in conditions Edaphoclimatic of the province of Manabi.&lt;/p&gt;

Deciphering functional groups of rumen microbiome and their underlying potentially causal relationships in shaping host traits.

Over the years, microbiome research has achieved tremendous advancements driven by culture-independent meta-omics approaches. Despite extensive research, our understanding of the functional roles and causal effects of the microbiome on phenotypes remains limited. In this study, we focused on the rumen metaproteome, combining it with metatranscriptome and metabolome data to accurately identify the active functional distributions of rumen microorganisms and specific functional groups that influence feed efficiency. By integrating host genetics data, we established the potentially causal relationships between microbes-proteins/metabolites-phenotype, and identified specific patterns in which functional groups of rumen microorganisms influence host feed efficiency. We found a causal link between Selenomonas bovis and rumen carbohydrate metabolism, potentially mediated by bacterial chemotaxis and a two-component regulatory system, impacting feed utilization efficiency of dairy cows. Our study on the nutrient utilization functional groups in the rumen of high-feed-efficiency dairy cows, along with the identification of key microbiota functional proteins and their potentially causal relationships, will help move from correlation to causation in rumen microbiome research. This will ultimately enable precise regulation of the rumen microbiota for optimized ruminant production.

Automatic Monitoring Methods for Greenhouse and Hazardous Gases Emitted from Ruminant Production Systems: A Review.

The research on automatic monitoring methods for greenhouse gases and hazardous gas emissions is currently a focal point in the fields of environmental science and climatology. Until 2023, the amount of greenhouse gases emitted by the livestock sector accounts for about 11-17% of total global emissions, with enteric fermentation in ruminants being the main source of the gases. With the escalating problem of global climate change, accurate and effective monitoring of gas emissions has become a top priority. Presently, the determination of gas emission indices relies on specialized instrumentation such as breathing chambers, greenfeed systems, methane laser detectors, etc., each characterized by distinct principles, applicability, and accuracy levels. This paper first explains the mechanisms and effects of gas production by ruminant production systems, focusing on the monitoring methods, principles, advantages, and disadvantages of monitoring gas concentrations, and a summary of existing methods reveals their shortcomings, such as limited applicability, low accuracy, and high cost. In response to the current challenges in the field of equipment for monitoring greenhouse and hazardous gas emissions from ruminant production systems, this paper outlines future perspectives with the aim of developing more efficient, user-friendly, and cost-effective monitoring instruments.

Assessing the combination efficiency of some unconventional feed resources with concentrates and Chloris gayana grass in mitigating ruminal methane production in vitro.

In a preliminary in vitro study, leaves of Acacia nilotica, Prosopis juliflora, Cajanus cajan, Leucaena leucocephala and seed kernel of Mangifera indica were identified as potential candidates in mitigating ruminal methane (CH4) production. The objective of the current study was to investigate the combination efficiency of these unconventional feeds with concentrate mix (CM) or Chloris gayana grass in CH4 reduction. Two feed combinations in different proportions were incubated in vitro with buffered rumen fluid at Hohenheim Gas test. In combination 1, C. gayana and CM were included as basal substrates, while in combination 2, A. nilotica, P. juliflora, C. cajan, L. leucocephala or M. indica seed kernel were included as CH4 reducing supplements at different proportions. The CH4 reducing potentials of feed combinations were presented as the ratio of CH4 to net gas production and expressed as percentage (pCH4). The pCH4 for CM and C. gayana was 16.7% and 16.9%, respectively, while it ranged from 3.18% in A. nilotica to 13.1% in C. cajan. The pCH4 was reduced (p < 0.05) from 14.6% to 9.39% when A. nilotica was combined with CM. In combination of L. leucocephala or C. cajan with CM, the pCH4 (p < 0.05) was reduced from 16.5% and 16.6% with the lowest proportion to 15.1% and 15.2% with the highest inclusion rate respectively. The combination of C. gayana with L. leucocephala or C. cajan reduced (p < 0.05) the pCH4 from 16.3% and 16.4% to 15.1% and 14.9% respectively. The pCH4 was reduced (p < 0.05) from 13.4% to 7.60% when A. nilotica was combined with C. gayana. Estimated digestible organic matter (dOM) and metabolizable energy (ME) increased (p < 0.05) with increasing proportions of M. indica seed kernel with CM or C. gayana. In conclusion, the combination of the basal substrates with unconventional supplements resulted in CH4 reduction without affecting the dOM and ME at lower inclusion rates. Animal-based experiments await to validate in vitro findings.

Seroprevalence and contributing factors of transboundary animal diseases in sheep and goats: a study in Peninsular Malaysia.

Diseases caused by small ruminant lentiviruses, Mycobacterium avium ssp. paratuberculosis (MAP), Schmallenberg virus, and peste des petits ruminants virus (PPR) is globally recognised as serious threats to the ruminant industry due to their potential to spread rapidly across boundaries. Despite their global distribution and negative impacts on ruminant production, there is a gap in knowledge of the current trends in their epidemiology among sheep and goat populations in Peninsular Malaysia. This study was therefore designed to fill the gap of knowledge concerning the seroprevalence and contributing factors of CAEV, paratuberculosis, SBV, and PPRV among small ruminants from selected flocks in Selangor, Negeri Sembilan, and Pahang states in Peninsular Malaysia. A cross-sectional study design was used to collect animal data and blood samples for serological assays simultaneously. The ID Screen (ID.VET, France) indirect ELISA screening tests were used to detect serum antibodies directed against CAEV/MVV (VISNAS Ver 0922), paratuberculosis (PARAS Ver 0516), SBV (SBVC Ver 1114) and PPRV (PPRC Ver 0821). There was 45.4% (95% CI = 40.74-50.74), 6.8% (95% CI = 4.66-9.69), 27.8% (95% CI = 23.35-32.77), and 2.6% (95% CI = 1.11-0.51) true seroprevalence for CAEV, paratuberculosis, SBV, and PPR, respectively. Geographical location and species were the risk factors for CAEV and paratuberculosis, while the management system and age of small ruminants were the risk factors for SBV. The present study is the first to document a large-scale seroprevalence of MAP and PPR infection among sheep and goat flocks in Peninsular Malaysia. The presence of PPRV and MAP antibodies among small ruminant flocks is signalling current or previous exposure to the pathogens or cross reactions with similar antigens. This finding further suggests the potential for future outbreaks of these devastating diseases among sheep and goats in Malaysia. The high seroprevalence of CAEV and SBV among small ruminants indicates high levels of exposure to the viruses in the environment, which is a potential threat to production.

Socio-Economic Structure, Production Practices, Observations and Suggestions in Nomadic Sheep Production in Ordu province located in Black-Sea Region of Türkiye

Products obtained from farm animals meet the need for animal protein, which has an important place in human nutrition. In recent years, the demand for the products of small ruminants has been increasing. Products obtained from small ruminants are important in meeting the nutritional needs of the world population. Especially in light of the effect of global warming, small ruminants production has become even more important. Especially considering the production conditions of small ruminants, the nutritional needs of animals are met by grazing in the existing highlands. This study was conducted in Ordu province, located in the Black Sea Region of Türkiye. The Black Sea region, with its coasts and springs, is a region where breeders engage in nomadic animal husbandry. In the study, it was determined that 86.6% of the nomadic sheep breeders are producing Karayaka breed sheep while 13.4% are breeding Karayaka crossbreds. In the study, 47.9% of the breeders who participated in the survey live in the village and 45.1% live in the district. Percent 94.4 of the breeders were male and 5.6% were female. It was determined that 32.4% of the breeders were between the ages of 41-50 and 40.8% were primary school graduates. The rate of those who contribute to their livelihood through husbandry is 57.2%. The rate of those who want to continue small ruminant farming in the future is 82.9%. Additionally, it was determined that the majority of breeders attach importance to record keeping and animal health protection practices. In the enterprises where the study was conducted, difficulties were encountered in supplying feed and finding shepherds, and high feed prices and labor costs were stated as the most common problems. In nomadic sheep breeding, grazing and climate parameters in pastures and plateaus have a significant impact on the nutrition of the animals. Therefore, facilitating the transportation of sheep to pastures and plateaus and the sustainability of these areas will positively affect nomadic sheep breeding.

Animal Behaviour Packs a Punch: From Parasitism to Production, Pollution and Prevention in Grazing Livestock.

Behaviour is often the fundamental driver of disease transmission, where behaviours of individuals can be seen to scale up to epidemiological patterns seen at the population level. Here we focus on animal behaviour, and its role in parasite transmission to track its knock-on consequences for parasitism, production and pollution. Livestock face a nutrition versus parasitism trade-off in grazing environments where faeces creates both a nutritional benefit, fertilizing the surrounding sward, but also a parasite risk from infective nematode larvae contaminating the sward. The grazing decisions of ruminants depend on the perceived costs and benefits of the trade-off, which depend on the variations in both environmental (e.g., amounts of faeces) and animal factors (e.g., physiological state). Such grazing decisions determine the intake of both nutrients and parasites, affecting livestock growth rates and production efficiency. This impacts on the greenhouse gas costs of ruminant livestock production via two main mechanisms: (1) slower growth results in longer durations on-farm and (2) parasitised animals produce more methane per unit food intake. However, the sensitivity of behaviour to host parasite state offers opportunities for early detection of parasitism and control. Remote monitoring technology such as accelerometers can detect parasite-induced sickness behaviours soon after exposure, before impacts on growth, and thus may be used for targeting individuals for early treatment. We conclude that livestock host x parasite interactions are at the centre of the global challenges of food security and climate change, and that understanding livestock behaviour can contribute to solving both.

Protein supplementation during mid-gestation affects maternal voluntary feed intake, performance, digestibility,and uterine blood flow of beef cows.

This study aimed to assess the impact of protein supplementation and its interaction with calf sex (CS) on the performance, metabolismand physiology of pregnant beef cows. Fifty-two multiparous Zebu beef cows carrying female (n = 22) and male (n = 30) fetuses were used. Cows were individually housed from day 100 to 200 of gestation and randomly assigned to restricted (RES, n = 26) or supplemented (SUP, n = 26) groups. The RES cows were ad libitum fed a basal diet (corn silage + sugarcane bagasse + mineral mixture), achieving 5.5% crude protein (CP), while SUP cows received the same basal diet plus a protein supplement (40% CP, at 3.5 g/kg of body weight). All cows were fed the same diet during late gestation. Differences were declared at p < 0.05. No significant interaction between maternal nutrition and calf sex was found for maternal outcomes (p ≥ 0.34). The SUP treatment increased the total dry matter (DM) intake (p ≤ 0.01) by 32% and 19% at mid- and late-gestationrespectively. The total tract digestibility of all diet components was improved by SUP treatment at day 200 of gestation (p ≤ 0.02), as well as the ruminal microbial CP production (p ≤ 0.01). The SUP treatment increased (p ≤ 0.03) the cows'body score condition, ribeye area, the average daily gain (ADG) of pregnant components (PREG; i.e., weight accretion of cows caused by pregnancy)and the ADG of maternal tissues (i.e., weight accretion discounting the gain related to gestation) in the mid-gestation. The SUP cows exhibited a lower maternal ADG (p < 0.01) compared to RES cows in late pregnancy. There was a 24% additional gain (p < 0.01) in the PREG components for SUP cows during late gestation, which in turn improved the calf birthweight (p = 0.05). The uterine arterial resistance and pulsatility indexes (p ≤ 0.01) at mid-gestation were greater for RES cows. In conclusion, protein supplementation during mid-gestation is an effective practice for improving maternal performance, growth of the gravid uterusand the offspring's birth weight.

Effect of Treating Date Peels with Different Levels of Yeast (Saccharomyces cerevisiae) on Some Fermentations of Rumen Fluid in Vitro

Introduction: The high cost and limited availability of traditional feeds at certain times of the year is one of the biggest constraints to animal production. This study aimed to demonstrate the effect of using dried date peels, and baker's yeast (Saccharomyces cerevisiae, S.s) in different incubation periods on rumen fermentations. Three levels of dried date peels (15%, 30%, and 60%), with different baker's yeast (Saccharomyces cerevisiae, S.C.) are used in this study in vitro condition. Results: The different treatments used in this study had a significant increase in ruminant gas production. Conclusion: Baker's yeast (Saccharomyces cerevisiae, S.C.) and date peels do not affect reducing rumen gas, indeed, these treatments increase the gas produced by the feed in vitro conditions.

A Meta-Analysis of the Effects of Dietary Betaine on Milk Production, Growth Performance, and Carcass Traits of Ruminants.

Betaine improves growth performance and health in monogastric animals under both thermoneutral and heat stress conditions, but results in ruminants have been more equivocal. This meta-analysis investigated the effects of betaine supplementation on productive performance, milk production and composition, and carcass traits of ruminants due to betaine supplementation. A comprehensive search for published studies investigating the effect of betaine was performed using Google Scholar, ScienceDirect, PubMed, and Scopus databases. Effect size analysis, random effects models, I2 statistics, and meta-regression analysis were utilized to assess differences in production parameters. Dietary betaine supplementation increased milk yield (+1.0 kg/d (weighted mean differences presented in this abstract), p < 0.001), dry matter intake (+0.15 kg/d, p < 0.001), and milk lactose (+0.05%, p = 0.010) in dairy cows housed under thermoneutral conditions. In the few studies conducted on small ruminants, there was an increase in milk yield in response to dietary betaine (0.45 kg/d, p = 0.040). Under heat stress conditions or grazing pasture during summer, dietary betaine increased milk yield (+1.0 kg/d, p < 0.001) and dry matter intake (+0.21 kg/d, p = 0.020). Dietary betaine increased final liveweight (+2.33 kg, p = 0.050) and back fat thickness (+0.74 cm, p < 0.001) in beef cattle. Dietary betaine increased final liveweight (0.14 kg, p = 0.010), daily gain (+0.019 kg/d, p < 0.001), and carcass weight (+0.80 kg, p < 0.001) but not backfat in small ruminants. These meta-analyses showed that dietary betaine increases liveweight in small ruminants and beef cattle and increases feed intake and milk yield in dairy cattle.

Characteristics of rumen microbiota and Prevotella isolates found in high propionate and low methane-producing dairy cows.

Ruminal methane production is the main sink for metabolic hydrogen generated during rumen fermentation, and is a major contributor to greenhouse gas (GHG) emission. Individual ruminants exhibit varying methane production efficiency; therefore, understanding the microbial characteristics of low-methane-emitting animals could offer opportunities for mitigating enteric methane. Here, we investigated the association between rumen fermentation and rumen microbiota, focusing on methane production, and elucidated the physiological characteristics of bacteria found in low methane-producing cows. Thirteen Holstein cows in the late lactation stage were fed a corn silage-based total mixed ration (TMR), and feed digestion, milk production, rumen fermentation products, methane production, and rumen microbial composition were examined. Cows were classified into two ruminal fermentation groups using Principal component analysis: low and high methane-producing cows (36.9 vs. 43.2 L/DMI digested) with different ruminal short chain fatty acid ratio [(C2+C4)/C3] (3.54 vs. 5.03) and dry matter (DM) digestibility (67.7% vs. 65.3%). However, there were no significant differences in dry matter intake (DMI) and milk production between both groups. Additionally, there were differences in the abundance of OTUs assigned to uncultured Prevotella sp., Succinivibrio, and other 12 bacterial phylotypes between both groups. Specifically, a previously uncultured novel Prevotella sp. with lactate-producing phenotype was detected, with higher abundance in low methane-producing cows. These findings provide evidence that Prevotella may be associated with low methane and high propionate production. However, further research is required to improve the understanding of microbial relationships and metabolic processes involved in the mitigation of enteric methane.

The potential of bioactive forage legumes for ruminant production in temperate and tropical areas: A One health approach

The potential of bioactive forage legumes for ruminant production in temperate and tropical areas: A One health approach