Rumex Nervosus Research Articles

Effect of Rumex nervosus leaves as a dietary supplement in broiler diets on the breast quality characteristics of broiler chickens

Effect of <i>Rumex nervosus</i> leaves as a dietary supplement in broiler diets on the breast quality characteristics of broiler chickens

Antibacterial activity and phytochemical screening of traditional medicinal plants most preferred for treating infectious diseases in Habru District, North Wollo Zone, Amhara Region, Ethiopia.

Ethiopia's healthcare system relies on traditional medicinal practices that use medicinal plants to treat human and livestock ailments. However, the lack of empirical validation regarding the efficacy of these treatments against specific infectious diseases necessitates rigorous scientific investigations. The objective of this study was to investigate the antibacterial activity and phytochemical screening on five selected medicinal plant species, namely Solanum somalense Franchet., Verbascum sinaiticum Benth., Rumex nervosus Vahl, Withania somnifera (L.) Dunal and Calpurnia aurea (Ait.) Benth. The plants were first identified jointly with local informants and later considering mainly their high informant consensus and fidelity level values for their efficacy in treating infectious diseases in the area. Ethanol and aqueous extracts were prepared from the plant materials, and their antibacterial activities were evaluated against standard bacterial strains, representing both gram-positive and gram-negative types. To assess the antibacterial activity of the extracts, the minimum inhibitory concentration (MIC) was determined using the broth dilution method. Additionally, phytochemical screening was performed using standard qualitative tests to identify various secondary metabolites. The results indicated antibacterial efficacy in the ethanol extracts of S. somalense, W. somnifera, and C. aurea against particular bacterial strains (S. somalense against S. agalactiae with MIC of 1.5 mg/mL; W. somnifera against S. aureus and E. coli, with MIC of 2 mg/mL; C. aurea against E. coli and K. pneumoniae, with MICs of 3 mg/mL and 3.5 mg/mL, respectively). The results of the phytochemical screening indicated the presence of steroids, alkaloids, flavonoids, saponins, and terpenoids. The selected medicinal plants demonstrated promising antibacterial activity against certain bacterial strains. The current findings support the long-standing claim of the traditional medical system of the study area for their continued use of these plants in their treatment of infectious diseases. Further investigation is required to isolate the responsible active compounds and characterize the constituents and description of their antibacterial effect for possible use in areas where these infectious diseases are major health problems.

Rumex nervosus nanoparticles versus aqueous extract: introduction of novel immunomodulatory anti-inflammatory nano-preparation for management of paw oedema and gastritis in rats

BackgroundRumex nervosus is abundant in East African and Arabian countries, and is used in curing gastrointestinal diseases as well as in wound healing. The current study intends to assess Rumex nervosus aqueous extract's anti-inflammatory benefits compared to its nanoparticles on rat paw edema and its gastric mucosal protection against ulceration.Materials and methodsIn-vitro cytotoxicity effects and antioxidant activity of Rumex nervosus nanoparticles versus aqueous extract were studied, followed by a pilot in vivo pharmacological study to determine the suitable dose of nanoparticles that would be used in the safety and efficacy studies in comparison with the aqueous extract. Its protective effects on arthritis and soft tissue inflammation were studied in rat paw edema and gastric ulcer models. Rumex nervosus extract (250 and 500 mg/kg) and nanoparticles (3.3 and 6.6 mg/kg) were given to four groups of rats orally before induction of paw oedema with subplantar 0.2 ml (1% w/v) formaldehyde or gastritis with oral ethanol 1 ml (70%), besides negative, positive control and reference groups.ResultsPaw volumes and gastric ulcer indices, as well as the anti-inflammatory and antioxidant parameters (kappa β, Paraoxonase1, and Malondialdehyde) that were measured in sera showed a marked reduction in groups treated with high doses of Rumex nervosus extract, and nanoparticles. Histopathologic and histochemical assessment of the stomachs confirmed the other investigations. All results were significant compared to positive control untreated groups.ConclusionsMost studies demonstrated Rumex nervosus’s protective anti-inflammatory benefits with the superiority of large doses of nanoparticles, offering a promising natural solution for low cost against inflammation.

Antioxidant activity of silver nanoparticles synthesized from crud methanolic extract of rumex nervosus

In the present investigation, an attempt was made to prepare nanoparticles by using a medicinal plant Rumex nervosus, because the biological synthesized nanoparticles have been widely used in the field of medicine now. Silver sulfate (Ag2SO4) was used for the synthesis of the silver nanoparticles using the root extract of Rumex nervosus. The synthesized silver nanoparticles from silver sulfate solution through the root extract were characterized using UV-vis spectrophotometry, SEM, and FT-IR methods. The SEM analysis showed the average size of 75 nm with spherical shape and it confirmed the formation of nanoparticles in the sample. Antioxidant activity of the crude extract was evaluated by employing the DPPH radical scavenging assay. The result showed moderate antioxidant activity at various concentration as compared to standard ascorbic acid solution. The synthesized nanoparticles can be used for various applications due to its eco-friendless, nontoxic and compatibility for pharmaceutical and other applications.

Identification and Establishing Floral Calendar in Jimma Zone of Oromia Ethiopia

Bee flora resources are crucial for the development of beekeeping. The study aimed to find and construct a flowering calendar for bee plants in order to produce many batches of honey. Data were collected through bee plant inventory, pollen collection and honey pollen analysis. Based on bee plant inventories, pollen load collection, and honey pollen analysis, 103 plant species were identified as nectar and pollen sources for honeybees. Ageratum conizoides, Guizotia scabra, Cordia africana, Datura arborea, Plantago lanceolata, Rumex nervosus, and Justicia schimperiana were the most common honey-producing plants in the area. The fresh weight of pollen gathered at various months of the year revealed that a substantial amount of pollen was collected in September, October, and November, but the incoming pollen weight decreased in March and July. The area's peak honey flow period began in November and lasted until early December or late February. The modest honey flow also occurred in May and June. Pollen examination of honey samples indicated three monofloral honey types: Guizotia spp., Vernonia, and Croton monofloral honey types, with pollen frequencies ranging between 45% and 100%. The study indicated that the floral calendar of bee forages is completely reliant on the seasonal availability of food sources, and beekeepers should be aware of how to manage honeybee colonies in accordance with the flowering calendar in their area in order to enhance the frequency of honey production.

Meat fatty acids profile including metabolic, qualitative, nutritional indices, and organoleptic evaluation as affected by Rumex nervosus leaves meal fortified broiler diets

The effects of dietary supplementation with Rumex nervosus leaves (RNL) on fatty acid (FAs) indices, organoleptic evaluation, performance index, and feed efficiency were investigated. This study consists of five treatments. Broilers in treatments 1–4 received basal feed plus RNL at doses of 0, 1, 3, and 5 g/kg, while broilers in treatment 5 received basal feed plus the antibiotic Colimex. On day 34, FAs profile and organoleptic assessment were evaluated, and performance index and feed efficiency were determined for the entire experimental period. Results showed that FAs profile of the breast meat was affected by treatments with varying degrees (p˂0.05). The 1 g RNL resulted in the highest eicosapentaenoic acid + docosahexaenoic acid, which resulted in the highest meat lipid quality, the highest elongation index, improved organoleptic characteristics, and tended to improve performance index, feed efficiency, and nutritional index. The 5 g RNL treatment reduced total saturated fatty acids (ƩSFAs), omega-6/omega-3 ratio, especially linoleic acid/α-linolenic acid ratio, alanine aminotransferase, and increased total omega-3 fatty acids, unsaturation index, and Δ9-desaturase. In conclusion, RNL can be introduced to broiler diets, especially at an incorporation rate of 1 g, and it promotes broiler health and performance because it contains phytoconstituents that can alter the content and indices of FAs meat and improve storability and sensory quality.

Ecology and Diversity of Angiosperm Parasites and Their Host Plants along Elevation Gradient in Al-Baha Region, Saudi Arabia

The ecology and diversity of flowering parasitic plants and their hosts are poorly investigated and usually ignored in Saudi Arabian plant communities. Therefore, this work aimed at assessing the ecology and diversity of parasitic plants and their hosts along an elevation gradient in the Al-Baha region (1300–2400 m.a.s.l.). Different quantitative vegetation parameters were applied to analyze the collected data. Eight parasitic plants from six genera and four families were identified along the gradient, with 67% of them being zoochorously dispersed species. They accounted for approximately 23.5% (8 out of 34) of those found throughout Saudi Arabia. Perennials, stem hemiparasites, and biregional taxa accounted for around 62.5% of the total parasites, whereas indigenous species accounted for 75%. The dominant family of parasitic species was Loranthaceae (50%), and Phragmanthera austroarabica A.G.Mill. &amp; J.A.Nyberg was the most important species (IVI = 107.28). Orobanche cernua Loefl. and Loranthella deflersii (Tiegh.) S.Blanco &amp; C.E.Wetzel were restricted to the dry zone (low elevation) only, while the other parasites were distributed across the surveyed region. Twenty-three host plants were identified throughout the study region. About 83% of them were phanerophytes and bioregional plants, with 91% being perennial species. The prevalent host plant family across all sites was Fabaceae, with Nicotina glauca Graham being the most important host species (IVI = 32.44%). P. austroarabica and Plicosepalus curviflorus Tiegh. preferred Vachellias as host plants, while Vachellia flava (Forssk.) Kyal. &amp; Boatwr. was the heavily infected host by P. austroarabica. P. austroarabica had a broad spectrum of host range (13 host plants), while O. cernua had a very narrow host range (only Rumex nervosus Vahl). Individual parasite and host species were markedly more abundant in the wet zone than in the low-altitude dry zone. Further research is needed to fully understand such distinctive groups of plants and their negative and positive ecological consequences on plant biodiversity and natural ecosystems.

Effects of Rumex nervosus leaf powder supplementation on carcasses compositions, small intestine dimensions, breasts color quality, economic feasibility in broiler chickens

Carcass characteristics, product quality, and the conversion of feed into meat with higher performance and feasibility are important determinants of the profitability and sustainability of any poultry enterprise. The objective of this study was to investigate the potential effect of Rumex nervosus leaf powder (RNLP) on carcass composition, small intestine dimensions, breast color quality, and economic feasibility of Ross broiler chickens. A total of 122 broilers were randomly distributed evenly among 4 treatments (0, 1, 3, and 5 g RNLP/ kg diet, n=5 replicates per treatment, with 6 broilers per replicate: 3 ♀ and 3 ♂). Performance and economic data were collected in each replicate during the experiment. One male bird from each replication was examined for other criteria on d 34. Results showed that broilers fed 3 g of RNLP had a higher eviscerated carcass weight and dressing percentage (P < 0.05) than the control. The RNLP-fed broilers had higher weights and thicknesses in the duodenum and jejunum of up to 5 g and in the ileum and overall small intestine of up to 3 g. Broilers fed the lowest levels of RNLP had the highest revenue and net gain and the lowest cost-benefit ratio compared with those fed the highest levels of RNLP. The results indicate that the addition of RNLP has a positive effect on carcass processing, the intestinal measurements, and profitability ratios of the broilers. The 1 g RNLP/kg feed is the recommended dose to increase productivity and economic profitability ratios per kilogram of meat.

Biosynthesis of Silver Nanoparticles and Exploring Their Potential of Reducing the Contamination of the In Vitro Culture Media and Inducing the Callus Growth of Rumex nervosus Explants.

Among biological methods, green synthesis of the nanomaterials using plant extracts was shown to be an environmentally friendly, economical, and simple approach. In the current study, the biogenic synthesis of silver nanoparticles (AgNPs) was achieved using the leaf extract of Hibiscus tiliaceus, in order to prevent the contamination of the tissue culture media and induce callus growth. The nanostructures of the fabricated AgNPs were characterized using UV-visible spectroscopy, Fourier transform infra-red spectra (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM), zeta size, and zeta potential techniques. Our results indicate that The UV-vis spectrum of AgNPs exhibited an absorption band at 415 nm. The FTIR analysis identified the functional groups which could involve in the reduction of silver ions to AgNPs, this was also confirmed by the (hkl) diffraction peaks in the XRD diffractogram. Moreover, the TEM analysis showed a spherical nanoparticle with a size ranging from 21 and 26 nm. Thereafter, the potential antibacterial and antifungal activity of the biogenic AgNPs was evaluated against Bacillus pumilus and Alternaria alternata which were isolated from the in vitro culture media and identified based on 16S rDNA and ITS rDNA sequences, respectively. The results showed that the AgNPs significantly inhibited the growth of Alternaria alternata and Bacillus pumilus at all applied concentrations (5, 10, 20 and 40 mg/L). Compared to the control more fungal radial growth reduction (42.59%,) and bacterial inhibition (98.12%) were registered in the plates containing high doses of AgNPs (40 mg/L). Using Rumex nervosus explants, the biosynthesized AgNPs were tested for their impact to promote callus growth. The obtained results showed a significant effect of AgNPs on callus fresh weight at all applied doses. Moreover, AgNPs treatments showed a polymorphism of 12.5% which was detected by RAPD markers. In summary, the results revealed that AgNPs (40 mg/L) can be effectively added to the in vitro culture media for reducing microbial contamination and improving callus growth while greatly maintaining its genetic stability.

Efficacy and Optimal Feeding Level of Rumex Nervosus Leaves on Blood Biochemistry, Carcass Characteristics, Productivity Indices, and Anticoccidial Indicators of Broiler Chickens Infected or Not Infected with Eimeria Tenella

Efficacy and Optimal Feeding Level of Rumex Nervosus Leaves on Blood Biochemistry, Carcass Characteristics, Productivity Indices, and Anticoccidial Indicators of Broiler Chickens Infected or Not Infected with Eimeria Tenella

In vitro phytochemical analysis and antibacterial and antifungal efficacy assessment of ethanolic and aqueous extracts of Rumex nervosus leaves against selected bacteria and fungi.

Scientists are interested in identifying natural antibiotic substitutes that are effective against drug-resistant pathogenic microbes and spoilage fungi to counter pathogens and reduce the major public health problem of antibiotic residues in animal products. This study aimed to evaluate the antimicrobial activity of Rumex nervosus leaves (RNLs) as a medicinal herb against four bacterial and two fungal strains using absolute ethanol, 50% ethanol, and aqueous extracts. The antimicrobial activities of various RNL extracts against selected microbes were evaluated using the disk diffusion antibiotic susceptibility test, minimum inhibitory concentrations (MICs), minimum bactericidal concentrations (MBCs), minimum fungicidal concentrations, and the poisoned food technique. The absolute ethanol RNL extract showed the best bacteriostatic/bactericidal activity against Salmonella Typhimurium, Escherichia coli, and Staphylococcus aureus (MIC/MBC: 0.20/0.40, 0.20/0.40, and 0.32/0.65 mg/mL, respectively). The diameter of the zone of inhibition was larger (p < 0.05) for the 100% ethanol RNL extract (8.17 mm) against Salmonella Typhimurium, the 50% ethanol-RNL extract (11.5 mm) against E. coli, and the aqueous RNL extract (14.0 mm) against S. aureus than for any other bacterial isolate. The aqueous RNL extract strongly (p < 0.0001) inhibited the mycelial growth of Aspergillus fumigatus (100%) and Aspergillus niger (81.4%) compared with the control. The results of this study suggest that RNL is a promising new natural antimicrobial agent for food preservation. To date, most research on the antimicrobial properties of natural herbs has been conducted in vitro, with few exceptions in vivo and intervention-based research.

Rumex nervosus mediated green synthesis of silver nanoparticles and evaluation of its in vitro antibacterial, and cytotoxic activity

In the present study, we have reported the low-cost, green synthesis of silver nanoparticles using Rumex nervosus (Rn) leaf extract. The biological synthesis of Rn-AgNPs employing plant extracts has become an optimistic substitute for the conventional chemical method of synthesis. Initially, the synthesis of Rn-AgNPs was explained by the optical study of the color change in the mixture. The biosynthesized AgNPs were characterized by UV-visible absorption spectroscopy, FTIR analysis, EDX, zeta potential, and DLS. TEM revealed that most of the Rn-AgNPs are spherical/oblong in shape with the average particle size falling in the range of 20-70 nm. The green synthesized Rn-AgNPs showed potent antibacterial and antibiofilm activity against the gram-negative (Escherichia coli) and gram-positive (Staphylococcus aureus) bacteria. The cytotoxicity assays of as-formed AgNPs exhibited minimal toxicity to human RBCs and PBMCs. Hence, these nanoparticles are considered non-toxic for use as drugs in biomedicine.

Antioxidant Activity and Anti-Nutritional Factors of Selected Wild Edible Plants Collected from Northeastern Ethiopia.

In Ethiopia, wild edible plants (WEPs) offer a natural food supply for humans to alleviate food insecurity and hunger. Despite the extensive usage of WEPs in Ethiopia, there have been few investigations on their nutritional composition. Our study aimed to evaluate the antioxidant activity and anti-nutritional factors of the most commonly consumed WEPs in Northeastern Ethiopia. The antioxidant parameters including total phenol, total flavonoid, 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Ferric Antioxidant Power (FRAP) assay contents and the anti-nutritional parameters including oxalate, phytate and tannin contents of the selected seven WEPs were evaluated using standard food analysis techniques. The total phenol (mg GAE/100 g) and total flavonoid (mg QE/100 g) content of WEPs resulted in ranges of 0.79–17.02 and 2.27–7.12, respectively. The antioxidant activity revealed that leaves of Amaranthus hybridus and Rumex nervosus have the highest DPPH and FRAP value, scavenging 50% of free radicals under 50 µg/mL. Non-food values resulted in the respective ranges of 3.37–11.73 mg/100 g oxalate, 16.31–165 µg/100 g phytate and 1.38–5.49 mg/100 g tannin. The investigation indicates that the antioxidant activity of WEPs under research was higher than common crops, and the non-food values were laid in the safe limit, indicating that these might be used for making more healthy and nutritious foods.

In Vitro Antioxidant and Anti-inflammatory Activities of Twenty-two Ethiopian Medicinal Plants

The current study has been carried out to assess the in vitro antioxidant and anti-inflammatory activities of the 80% ethanolic extracts of 22 traditionally used Ethiopian medicinal plants. Among the tested plants, Dodonaea angustifolia, Rumex nervosus, Carrisa spinarum, Pentas schimperiana, and Clerodendrum myricoides displayed good DPPH radical scavenging potential with IC50 values of 15.0, 26.9, 58.3, 62.5, and 78.8 µg/ml, respectively. Similarly, Dovyalis abyssinica, D. angustifolia, Rumex abyssinicus, and R. nervosus showed strong oxidative burst inhibitory potential with IC50 values of 10.2, 19.4, 24.8, 35.8 μg/ml, respectively. Furthermore, D. angustifolia and R. nervosus showed strong reactive oxygen species inhibitory activities. The results of this study showed the potential use of herbal products as anti-inflammatory and antioxidant agents and provided scientific backing for utilizing these herbal preparations.

Antioxidant and antimicrobial potential of two extracts from Capparis spinosa L. and Rumex nervosus and molecular docking investigation of selected major compounds

The present study examined the phytochemical composition, antioxidant, antimicrobial properties, and molecular docking of different solvents extracts (methanol and water) of two medicinal plants, namely, Capparis spinosa L (CS) and Rumex nervosus (RN). Phytochemical analysis showed that total phenol, flavonoids, alkaloids, and vitamin C were significantly (P ≤ 0.05) higher in the methanolic extract of both plants than in other solvents. However, tannin content was significantly (P ≤ 0.05) high in the water extract for both plants. Chloroform and acetone extracts were significantly lower in phytochemicals than other solvents, therefore excluded in this study. GC–MS analysis showed one dominant compound in CS (isopropyl isothiocyanate) and two in RN (pyrogallol and palmitic acid). The antioxidant methods applied (DPPH, ABTS, β-Carotene/linoleic acid assay, and reducing the power) showed that the methanolic extract of CS exerted higher activity in methanolic extract but lower than that of BHA standard. The methanolic extract of both plants inhibited the bacterial pathogens when a minimum inhibitory concentration (MIC) method was applied, compared to water extract with RN-methanolic extract had a lower inhibition concentration than CS-methanolic extract. The molecular interactions study revealed that the palmitic acid and pyrogallol interacted with the receptors' active site. This work concluded that CS and RN showed a remarkable antioxidant and antibacterial effect with the high antimicrobial activity of RN extract.

Radical scavenging potency, HPLC profiling and phylogenetic analysis of endophytic fungi isolated from selected medicinal plants of Saudi Arabia

BackgroundEndophytic fungi are associated within plant tissues without any harmful effects. They produce a diversity of metabolites and are well known as rich source of bioactive substances. This study aimed to identify fungal isolates from different medicinal plants, evaluate their antioxidant potency and detect components responsible for antioxidant activity. ResultsIn the present investigation, 4 endophytic fungal isolates were obtained from different medicinal plants Rumex nervosus, Pulicaria crispa and Withania somnifera. Those isolates were characterized as Penicilluim commune, Penicilluim glaucoroseum, Aspergillus flavipes and Fusarium chlamydosporum according to morphological and phylogenetic bases. These strains were propagated on humified rice and their crude ethyl acetate extracts were assayed for their total phenols, flavonoids and antioxidant potency against DPPH free radicals. The results showed that the elevated constituent of phenolics was found in P. commune (148.24 mg/g), followed by F. chlamydosporum (124.25 mg/g), while the highest total flavonoids were determined in A. flavipes, followed by F. chlamydosporum, P. glaucoroseum, P. commune, respectively. Moreover, the maximum hydroxyl radical-scavenging assay was IC50 = 2.13 mg/mL exhibited by P. commune, followed by F. chlamydosporum (2.68 mg/mL). Weak antimicrobial and cytotoxic effects were reported by the studied strains against pathogenic isolates and (HepG2) hepatocellular carcinoma cell line. The fungal EtOAc extracts were characterized quantitatively by the incidence of different phenols, flavonoids, and phenolic acids, as detected by HPLC. ConclusionsEndophytic fungi derived from medicinal plants are a promising candidate for the wide industrial production of antioxidant agents with several useful medical and pharmaceutical applications.How to cite: Hassane A. M. A, Taha T. M, Awad M. F, et al. Radical scavenging potency, HPLC profiling and phylogenetic analysis of endophytic fungi isolated from selected medicinal plants of Saudi Arabia. Electron J Biotechnol 2022;58. https://doi.org/10.1016/j.ejbt.2022.05.001

Efficacy of Rumex nervosus leaves or Cinnamomum verum bark as natural growth promoters on the growth performance, immune responsiveness, and serum biochemical profile of broiler chickens

This study was conducted to evaluate the potential growth and health promotion of Rumex nervosus leaves (RNL) and Cinnamomum verum bark (CVB) supplementation as phytogenic growth promoters on broilers. For 34 days, 540 day-old broiler chicks were randomly assigned to nine groups. Birds were fed the basal diet supplemented with 1, 3, or 5 g/kg RNL, 2, 4, or 6 g/kg CVB. In addition, birds were fed the basal diet complemented with antibiotic (AGP), or basal diet without feed additive as controls. All bird vaccinated for IBV, IBDV, and NDV except negative control (NC). The selected indicators were measured and analysed. Broilers given 1 g RNL were numerically heavier at 34 days and gained more to a degree comparable to the AGP group (p = .053). The addition of CVB at 2 g resulted in the best-feed conversion up to 21 d (p = .04). Throughout the study, dietary treatments had no impact on feed intake. Except for ALT, none of the immune-related parameters or serum biochemical profile differed statistically between treatments when compared to vaccinated control group. When comparing the non-vaccinated un-treated group on day 34, vaccination increased significantly antibody titres to NDV and IBDV. In conclusion, dietary RNL and CVB, especially at low doses, attempted to maximise the performance without compromising health in broiler chickens under typical conditions. Further research into the use of natural herbs under challenging conditions is required to maximise performance, maintain animal productivity, and achieve the ultimate goal of reducing antibiotic use in the poultry industry. Highlights Cinnamomum verum bark at a dose of 2 g/kg diet improved feed conversion ratio during the starter period. However, both selected herbs result unsatisfactory improvements in broiler performance at overall period under the ideal hygienic conditions. If broilers are exposed to bacterial challenges, phytogenic feed additives or even antibiotics may manifest their activity in promoting animal health and, as a result, growth performance. Low levels of hepatic enzymes at day 34 of age showed that birds’ health was good. Cinnamon powder improves kidney function at day 21 by lowering serum creatinine levels with increasing dose (p < .05). Rumex nervosus leaves or Cinnamomum verum bark have hypoglycaemia activity on day 31 by lowering serum glucose levels as the dose is reduced.

Rumex nervosus could alleviate streptozotocin-induced diabetic nephropathy in rats by activating Nrf2 signaling.

This study tested the protective effect of Rumex nervous (R. nervosus) methanol extract against streptozotocin (STZ)-mediated type 1 diabetes mellitus (T1DM)-induced nephropathy in rats and examined if this protection involves activating the nuclear factor erythroid 2 related factor-2 (Nrf2). Rats were divided into control, R. nervous (300 mg), STZ (T1DM), STZ + R. nervosus (100, 200, or 300 mg/kg), and STZ + R. nervosus (300 mg/kg) + brusatol (an Nrf2 inhibitor). With no effect on fasting glucose and insulin levels, R. nervosus methanol extract preserved kidney histological structure and alterations kidney function markers (e.g. albumin, creatinine, and urine volume) in the STZ-diabetic rats. R. nervosus also reduced levels of reactive oxygen species (ROS), malondialdehyde (MDA), tumor necrosis factor-α (TNF-α), and interleukine-6 (IL-6), nuclear levels of the nuclear factor kappa beta (NF-κB), and mRNA of caspase-3 and Bax in the kidneys of these diabetic rats. Concomitantly, it stimulated renal mRNA levels of Bcl2 and Nrf2, cytoplasmic and nuclear levels of Nrf2, and levels of glutathione (GSH), catalase (CAT), and superoxide dismutase (SOD). All these effects were dose-dependent, with the maximum effect seen with the 300 mg/kg dose, all prevented by brusatol. Also, these effects occurred without any alteration in the transcription of the Kelch-like ECH-associated protein 1 (keap-1). Similar effects on levels of GSH, SOD, CAT, and NF-κB, as well as expression of Nrf2, were also observed in the kidney of control + R. nervous-treated rats. In conclusion, R. nervosus prevents diabetic nephropathy in rats by upregulating and activating Nrf2.

Evaluation of the anticoccidial effect of traditional medicinal plants, Cinnamomum verum bark and Rumex nervosus leaves in experimentally infected broiler chickens with Eimeria tenella

Rumex nervosus leaves (RNL) and Cinnamomum verum bark (CNB), phytogenic herbs, have received much attention in recent years for their antimicrobial properties; however, there is limited knowledge about their potential anticoccidial functions. The prophylactic effects of RNL and CNB were compared with salinomycin, a standard synthetic anticoccidial agent, in broilers experimentally infected with Eimeria tenella (E. tenella). One-day-old broiler chicks (n = 225) were randomly divided into nine groups. Birds were either fed a basal diet containing 1, 3, or 5 g RNL or 2, 4, or 6 g CNB/kg feed, or treated with salinomycin within the basal diet, or the infected (IUT) or non-infected (UUT) groups were fed a basal diet only. Birds infected (n = 25 bird/group) with 40,000 sporulated E. tenella oocysts/bird at d 21 except UUT. Bodweight gain (BWG) and feed conversion ratio (FCR) were significantly (p < .05) lower in IUT compared to UUT. On day 7 post-infection (DPI), birds treated with RNL, CNB, or salinomycin had fewer lesions in the caeca and a lower oocyst value, and a higher oocyst reduction rate in the faeces than birds in the IUT. Although RNL was not able to reduce weight loss caused by coccidiosis, CNB at 6 g improved FCR and production efficiency index (PEI) at 7 DPI compared to the infected groups. In conclusion, RNL at 5 g and CNB at 6 g have moderate anti-coccidial activity and could be used to treat poultry coccidiosis in the field. However, more research is needed to identify active ingredients that make it effective compared to commercially available drugs. HIGHLIGHTS Phytogenic feed additives prevented weight loss and caecum pathology in broiler chickens at risk of coccidiosis. Rumex nervosus leaves and Cinnamomum verum bark had similar effects to the coccidiostat salinomycin at high doses. Traditional medicinal plants are potential alternatives to pharmaceutical coccidiostats to promote the health and growth of broiler chickens.

Antimicrobial Activities and Molecular Signature of Endophytic Fungi of Wild Plant, Saudi Arabia

Endophytic fungi, which have been reported in numerous plant species, are essential components of the wild plants’ community and contribute significantly to natural ecosystems' diversity. The study aims to evaluate and characterize, at the molecular level, the diversity and antimicrobial activities of endophytic fungi from wild plants in Taif, Saudi Arabia. Fungal endophytes were isolated from leaves and stems of plants, including Juniperus excelsa, Ziziphus spina-christi, Aacacia gerradii, Rumex nervosus, Lavandula dentate, Hypoestes forskaolii, Ficus palmata, Eucalyptus smithii and Conyza bonariensis collected from Taif, Saudi Arabia. About 25 different species belonging to 16 genera were recovered. The ribosomal DNA internal transcribed spacer (ITS) regions of the collected endophytic isolates were sequenced. The most prevalent fungi were identified as Hypocopra, Aspergillus, Coniothyrium, Chaetomium, Bipolaris, Dothideomycetes, Penicillium, Phoma, Mucor, Readeriella, Leptosphaerulina, Neodidymelliopsis, and Thielavia. Seven of the endophytic fungi isolates exhibited vigorous antimicrobial activity to some microbial pathogens tested. Fungal sp. has the highest inhibition against the human pathogenic bacteria Escherichia coli, Candida albicans, Staphylococcus aureus, Enterococeus faecium and Streptococcus agalactiae