Rumen Of Goats Research Articles

Rumen Cannulation of the Red Sokoto (Maradi) Goats for Nutritional Studies in Nigeria

TWO techniques for cannulating the rumen of the Red Sokoto goat of Nigeria are described. Stump type cannulae made of rigidblack., vulcathene1 material with screwable corks were found to be more suitable than the flexible cannulae made of plastisol2. They appeared to be well tolerated and remained in situ inspite of the animals’ nervous temperament and somewhat flabby abdominal wall. Experiences with some forty five of such cannulations performed between October 1970 and January 1975 are reported. These showed that although the double abdominal wall incision approach offered a better technique for cannulae implantation into the rumen of goats, strict routine post-operative attention was essential to avoid a range of post cannulation problems, such as pericannulae necrosis and infection, excessive leakage of the rumen liquor and partial or complete rejection of feed by the cannulated animals consequent upon such complications.

Functional screening of a Caatinga goat (Capra hircus) rumen metagenomic library reveals a novel GH3 β-xylosidase.

Functional screening of metagenomic libraries is an effective approach for identification of novel enzymes. A Caatinga biome goat rumen metagenomic library was screened using esculin as a substrate, and a gene from an unknown bacterium encoding a novel GH3 enzyme, BGL11, was identified. None of the BGL11 closely related genes have been previously characterized. Recombinant BGL11 was obtained and kinetically characterized. Substrate specificity of the purified protein was assessed using seven synthetic aryl substrates. Activity towards nitrophenyl-β-D-glucopyranoside (pNPG), 4-nitrophenyl-β-D-xylopyranoside (pNPX) and 4-nitrophenyl-β-D-cellobioside (pNPC) suggested that BGL11 is a multifunctional enzyme with β-glucosidase, β-xylosidase, and cellobiohydrolase activities. However, further testing with five natural substrates revealed that, although BGL11 has multiple substrate specificity, it is most active towards xylobiose. Thus, in its native goat rumen environment, BGL11 most likely functions as an extracellular β-xylosidase acting on hemicellulose. Biochemical characterization of BGL11 showed an optimal pH of 5.6, and an optimal temperature of 50°C. Enzyme stability, an important parameter for industrial application, was also investigated. At 40°C purified BGL11 remained active for more than 15 hours without reduction in activity, and at 50°C, after 7 hours of incubation, BGL11 remained 60% active. The enzyme kinetic parameters of Km and Vmax using xylobiose were determined to be 3.88 mM and 38.53 μmol.min-1.mg-1, respectively, and the Kcat was 57.79 s-1. In contrast to BLG11, most β-xylosidases kinetically studied belong to the GH43 family and have been characterized only using synthetic substrates. In industry, β-xylosidases can be used for plant biomass deconstruction, and the released sugars can be fermented into valuable bio-products, ranging from the biofuel ethanol to the sugar substitute xylitol.

Evaluación de fibra detergente neutra indigestible con incubación en diferentes especies

Objetivo do presente estudo foi avaliar a concentração da fibra em detergente neutro indigestível (FDNi) após a incubação ruminal em bovinos, caprinos e ovinos, e comparar os diferentes resultados com os métodos de estimativa e relacionar os dados com as análises químicas. Quatros forragens (silagem de milho, cana-de-açúcar, Brachiaria decumbens cv. Marandu e Panicum maximum cv. Tanzânia) dois concentrados (milho em grão e farelo de soja), um subproduto (casquinha de soja) e três amostras de fezes (bovino, caprino e ovino) foram incubadas em novilhos Nelore, cabras Saanen e ovinos Santa Inês. Os animais foram previamente adaptados a dieta experimental por 8 dias e incubação ocorreu por 240 horas. A concentração de FDNi foi mais alta nas amostras incubadas no rúmen de caprinos comparada com amostras incubadas no rúmen de bovinos. A incubação nos ovinos aumentou a concentração de FDNi nas forragens e tendeu a aumentar no concentrado e subproduto em relação a incubação no rúmen de bovinos. A concentração de FDNi foi similar para incubação em ovinos e caprinos. Em adição, as fezes bovinas tiveram alta concentração de FDNi, e as fezes de caprinos tiveram menor concentração de FDNi em relação às fezes de ovinos. O modelo CNCPS subestimou a concentração de FDNi nas amostras. Estimativas da FDN não degradável por Conrad et al. (1984) subestimou a concentração de FDNi na cana-de-açúcar e superestimou a concentração de FDNi no P.maximume concentrados. Em geral, a concentração de lignina foi ocomponente das amostrasmais relacionado com a concentração de FDNi. FDA foi a melhor para forragens (R2 = 0.668) e FDN foi melhor para concentrados (R2 = 0.454). Em conclusão, os processos digestivos das diferentes espécies afetam a concentração de FDNi do alimento. Erros nas estimativas dos modelos foram consideráveis e as características dos alimentos afetam a composição química e concentração de FDNi.

Rumen characteristics and blood parameters of West African dwarf goats fed vetiver grass (Chrysopogon zizanoides. L. Roberty) ensiled with cassava peels at different ratio

Vetiver is a perennial grass of tropical origin, commonly used as edge row plants for the control of erosion. Being leafy and with a high tillering ability, it has not been properly integrated as forage for ruminants. Thus, an experiment was carried out to determine the effect of ensiling a four week re-growth of vetiver grass with cassava peels on rumen and blood metabolites of West African dwarf goats. Sixteen goats were used for the study. A completely randomized design was used with four animals per treatment. Vetiver grass was ensiled with cassava peels at ratio (A) 80:20, (B) 70:30, (C) 60:40 and (D) 50:50 were provided ad-libtum at 5% body weight. Rumen and blood samples were collected at the end of the experiment which lasted for 90 days. Results of the study showed rumen ammonia nitrogen (NH3-N) concentration of 7.25-7.93mg/100mL, and pH of 6.75-6.81. Variations among the treatments were within the acceptable range for rumen microbial activity. There was a uniformly low plasma urea (4.36-5.16mm/l). Glucose (85.36-89.04g/dL), total protein (6.42- 7.50g/dL), cholesterol (60.95-66.19g/dL), haemoglobin (10.9-12.7g/100ml), packed cell volume (30.0-31.0%) and white blood cell (10.25-10.67×103/ul) were within the range reported for healthy goats. Ensiled vetiver grass with cassava peels had no detrimental effects on the rumen and blood parameters of the experimental animals.

Effects of fermented Yupingfeng on intramuscular fatty acids and ruminal microbiota in Qingyuan black goats.

Our previous work has demonstrated that Yupingfeng, a Chinese herb medicine, considered as prebiotic showed beneficial properties in poultry health and disease prevention and regulated intestinal microbiota. The effects of Yupingfeng on fatty acids related to meat flavor and ruminal microbiota are not yet known in Qingyuan black goat. In this study, we supplemented fermented (FYP) and unfermented (UYP) Yupingfeng in different combinations to 90 goats. Compared with the normal control group, FYP and UYP significantly increased the concentration of palmitic acid, octadecanoic acid, and arachidonate acid (related to meat flavor) in the longissimus dorsi muscle (p<.05). In addition, the significant upregulation of peroxisome proliferator-activated receptor gamma (PPARγ) and fatty acid translocase (CD36) was observed in the FYP and UYP groups (p<.05). In addition, Firmicutes and Bacteroidetes were the most abundance in goat rumen. At the genus level, FYP and UYP significantly increased Ruminococcus related to fiber degradation, and Alistipes related to short-chain fatty acids production. In summary, Yupingfeng could improve fatty acids of goat meat, which is probably triggered by the increase of PPARγ and CD36, and microbial activity. Besides, FYP showed more beneficial effects than UYP, with increased flavor fatty acids and beneficial microbes.

Comparison of microbial diversity in rumen and small intestine of Xinong Saanen dairy goats using 16S rRNA gene high-throughput sequencing

Context Gastrointestinal microorganisms play an important role in ruminant digestion and metabolism, immune regulation and disease prevention and control. Different parts of the digestive tract have different functions and microbial community structures. Aims This study aims to explore the microbial diversity in the rumen and the small intestine of Xinong Saanen dairy goats. Methods Rumen fluid and jejunum fluid from three Xinong Saanen dairy bucks with the average slaughter weight of 33.93 ± 0.68 kg were collected and analysed for microbial diversity, by using 16S rRNA gene high-throughput sequencing. Key results In total, 1118 operational taxonomic units (OTUs) were identified, with 1020 OTUs and 649 OTUs being clustered to rumen and jejunum samples respectively. Alpha-diversity indices were significantly (P &lt; 0.05) different between rumen and jejunum, as indicated by the fact that the rumen microbial community diversity, richness and uniformity/evenness were higher than those of jejunum. At the phylum level, the dominant phyla in the rumen were Bacteroidetes (66.7%) and Firmicutes (25.1%), accounting for 91.8% of the rumen microorganisms. The dominant phylum in the jejunum was Firmicutes, accounting for 73.0% of the jejunum microorganisms. At the genus level, the dominant bacteria in the rumen were Prevotella_1, norank_f_Bacteroidales_BS11_gut_group, Rikenellaceae_RC9_gut_group, Christensenellaceae_R-7_group and Family_XIII_AD3011_group, whereas the dominant bacteria in the jejunum were Omboutsia, Aeriscardovia, Intestinibacter, unclassified_f_Peptostreptococcaceae and unclassified_f_Bifidobacteriaceae. Clusters of Orthologous Groups (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) results showed that the major functions of microorganisms in the rumen and jejunum were carbohydrate metabolism, amino acid metabolism, nucleotide metabolism, membrane transport and translation. Interestingly, fructose and mannose metabolism and peptidoglycan biosynthesis were abundant in the rumen, while homologous recombination and nucleotide excision repair were abundant in the jejunum. Conclusions Our study clarified the differences in microbial diversity and community structure between the rumen and the jejunum in Xinong Saanen dairy goats. Prevotella was the most predominant genus in the rumen, compared with Romboutsia, Bifidobacterium as well as Peptostreptococcaceae genera, which were the predominant genera in the jejunum. Implications In combination with the functional prediction of microorganisms and the metabolic characteristics of different parts of the digestive tract in ruminants, our findings provided information for further exploring the relationship among genes, species and functions of microorganisms and their hosts’ nutritional and physiological functions.

A novel identified Pseudomonas aeruginosa, which exhibited nitrate- and nitrite-dependent methane oxidation abilities, could alleviate the disadvantages caused by nitrate supplementation in rumen fluid fermentation.

After the occurrence of nitrate-dependent anaerobic methane oxidation (AMO) in rumen fluid culture was proved, the organisms that perform the denitrifying anaerobic methane oxidizing (DAMO) process in the rumen of dairy goat were investigated by establishing two enrichment culture systems, which were supplied with methane as the sole carbon source and NaNO3 or NaNO2 as the electron acceptor. Several Operational Taxonomic Units (OTU) belonging to Proteobacteria became dominant in the two enrichment systems. The identified Pseudomonas aeruginosa, which was isolated from the NaNO2 enrichment system, could individually perform a whole denitrifying anaerobic methane oxidizing process. Further invitro rumen fermentation showed that supplementation with the isolated P.aeruginosa could reduce methane emissions, alleviate the nitrite accumulation and prevent the decrease in propionic acid product caused by nitrate supplementation.

Intake and rumen parameters in goats fed avocado (Persea americana Mill.) pulp and oil

The objective of this study was to examine the effect of supplementing goat diets with avocado pulp and oil, with respective ether extract (EE) contents of 14 and 6% (dry matter [DM] basis), on the intakes of DM and nutrients and on the rumen fermentation parameters of pH, ammonia (N-NH3), and volatile fatty acids (VFA). Non-pregnant, non-lactating, rumen-cannulated Saanen goats with an average weight of 66.6±4.6 kg were distributed into two 3 × 3 Latin squares. Concentrate DM intake (g/day) was higher in the animals that consumed the diet with pulp than in the goats fed the control and oil treatments. However, this higher concentrate intake was not sufficient to influence total diet DM intake or the intakes of crude protein, EE, mineral matter, neutral detergent fiber, acid detergent fiber, and total digestible nutrients (TDN). All evaluated rumen parameters were influenced by collection time, except N-NH3, which was influenced by the diets, with lower concentrations obtained with the pulp diet in relation to the control and oil treatments, yet within the limits considered normal to maximize DM intake and digestion. The pulp diet provided a lower butyric acid content than control at the collection times of two and eight hours after the meal, which was attributed to the proportional reduction of roughage in relation to concentrate. Diets with 6% EE do not influence intake or rumen parameters in non-pregnant, non-lactating goats. However, diets with 14% EE (DM basis) induce a reduction in the proportional intake of roughage in relation to concentrate and in the butyric acid and N-NH3 contents in the goat rumen.

Goats fed with non-protein nitrogen: ruminal bacterial community and ruminal fermentation, intake, digestibility and nitrogen balance

AbstractThe current study assessed ruminal fermentation parameters and bacterial community, nutrient intake, nutrient digestibility and nitrogen balance of goats fed diets containing buffel grass hay and ruminal ammonia nitrogen (N-NH3). Five rumen-cannulated mixed-breed castrated adult goats (45 ± 2.3 kg) were used in a 5 × 5 Latin square design represented by five N-NH3 levels (3.43, 9.95, 17.2, 23.0 and 33.7 mg/dl). Control animals were fed hay exclusively. Other treatments were represented by ruminal infusion composed of a mixture containing urea, ammonium sulphate and casein. The increasing N-NH3 concentrations did not affect rumen fluid pH, which averaged 6.43. Rumen ammoniacal nitrogen increased linearly in response to N-NH3. Volatile fatty acids were not affected by increasing N-NH3 concentrations. A higher abundance of Ruminococcaceae (Ruminococcus 1, Ruminococcaceae UCG-014 and Ruminococcaceae NK4A214 group) was observed in the rumen of goats infused with higher concentrations of N-NH3 (17.2 and 33.7 mg/dl N-NH3). There was a quadratic effect (P &lt; 0.050) of N-NH3 levels on neutral detergent fibre intake with maximum values estimated at 13.7 mg/dl N-NH3. Nutrient intake, nitrogen excretion and nitrogen balance presented a positive linear effect (P &lt; 0.050). In conclusion, 3.43 mg/dl of N-NH3 is the minimum level to maintain microbial activity, whereas the recommended level to optimize the microbial community is 14.5 mg/dl of N-NH3 in the rumen of goats fed buffel grass.

Comparison of MicroRNA Transcriptomes Reveals the Association between MiR-148a-3p Expression and Rumen Development in Goats.

Simple SummaryIn ruminants, the rumen epithelium plays an important role in nutrient absorption, metabolism and transport. MicroRNAs (miRNAs) have been reported to regulate the proliferation of diverse epithelial cells. In this study, we profiled the miRNA transcriptomes of goat rumens at four development stages and screened for candidate miRNAs related to rumen development. MiR-148a-3p was found to be highly expressed in the rumen tissues and induced the proliferation of GES-1 cells by targeting QKI. Our findings provide some insights into the functional roles of miRNAs in rumen growth and functional development in ruminants.The rumen is an important digestive organ of ruminants. From the fetal to adult stage, the morphology, structure and function of the rumen change significantly. However, the knowledge of the intrinsic genetic regulation of these changes is still limited. We previously reported a genome-wide expression profile of miRNAs in pre-natal goat rumens. In this study, we combined and analyzed the transcriptomes of rumen miRNAs during pre-natal (E60 and E135) and post-natal (D30 and D150) stages. A total of 66 differentially expressed miRNAs (DEMs) were identified in the rumen tissues from D30 and D150 goats. Of these, 17 DEMs were consistently highly expressed in the rumens at the pre-weaning stages (E60, E135 and D30), while down-regulated at D150. Noteworthy, annotation analysis revealed that the target genes regulated by the DEMs were mainly enriched in MAPK signaling pathway, Jak-STAT signaling pathway and Ras signaling pathway. Interestingly, the expression of miR-148a-3p was significantly high in the embryonic stage and down-regulated at D150. The potential binding sites of miR-148a-3p in the 3′-UTR of QKI were predicted by the TargetScan and verified by the dual luciferase report assay. The co-localization of miR-148a-3p and QKI through in situ hybridization was observed in the rumen tissues but not in the intestinal tracts. Moreover, the expression of miR-148a-3p in the epithelium was significantly higher than that in the other layers of the rumen, suggesting that miR-148a-3p is involved in the development of the rumen epithelial cells by targeting QKI. Subsequently, miR-148a-3p inhibitor was found to induce the proliferation of GES-1 cells. Taken together, our study identified DEMs involved in the development of the rumen and provides insights into the regulation mechanism of rumen development in goats.

Nutritional Value of Dried Rumen Digesta from Cattle, Sheep and Goat: A case of Bolgatanga Abattoir, Ghana

Purpose: The work evaluated the nutritive value of dried rumen digesta (DRD) from cattle (CRD), sheep (SRD) and goat (GRD) in Bolgatanga Abattoir, Ghana.Methodology: Rumen digesta (RD) was collected from twenty-seven (27)) animals, nine each from cattle, sheep and goat at the Bolgatanga Municipal Abattoir and sundried for 4 days. Proximate analysis, cell wall component and metabolizable energy were estimated after which the data was subjected to analysis of variance for statistical difference at 0.05% level.Findings: Result obtained showed significant differences (P&lt; 0.05) in proximate compositions. Goat Rumen Digesta (GRD) had highest organic matter (90.81%), Crude protein (12.17%) and metabolizable energy (ME) (374.24 Kca/100g) compared to the other experimental digesta from cattle. The Neutral detergent fibre (NDF), acid detergent fibre (ADF) and cellulose were not significantly different across the rumen digesta obtained from the species of animals.Unique contribution to theory, practice and policy: The values for organic matter and crude compositions obtained from the Sun-dried rumen digesta from cattle, sheep and goat indicated the ability of rumen digesta to provide the nutrients particularly protein required by animals for normal physiological activities. Farmers can process the digesta and include it in livestock feeds.

Effects of Bacillus amyloliquefaciens and Bacillus pumilus on Rumen and Intestine Morphology and Microbiota in Weanling Jintang Black Goat.

Simple SummarySelecting Bacillus amyloliquefaciens and Bacillus pumilus in the present work, antibiotic substitutes were found for weaned black goat health and growth. This study indicated that Bacillus amyloliquefaciens fsznc-06 and Bacillus pumilus fsznc-09 improved the development of rumen and small intestine in weanling Jintang black goats. They also regulated microbial communities by increasing the richness of beneficial bacteria, but decreasing that of pathogenic bacteria in rumen and caecum for nutrient metabolism and promoting healthy conditions.The importance of Bacillus as feed additives in animals’ production is well recognized. Bacillus amyloliquefaciens and Bacillus pumilus are involved in promoting animal growth performance and immunological indicators. However, their precise roles in the modulation of microbiota and immune response in goat rumen and intestines have not been investigated. The aim of the current work was to evaluate the impacts of Bacillus amyloliquefaciens fsznc-06 and Bacillus pumilus fsznc-09 in the development of rumen and small intestinal and microbial communities in rumen and caecum of weanling Jintang black goats. Morphological alterations of rumen and small intestine (duodenum, jejunum, and ileum) were evaluated by histochemical staining, and ruminal contents and cecal feces were analyzed by 16S rRNA sequencing in an Illumina NovaSeq platform. Morphological analysis showed that feeding weanling goats with Bacillus amyloliquefaciens fsznc-06 or Bacillus pumilus fsznc-09 enhanced ruminal papilla and small intestinal villus growth. In addition, 16S rRNA sequencing analysis indicated that microbial richness and diversity (Shannon, Simpson, Chao1, and ACE) and the relative richness of multiple or potential beneficial bacteria were higher in weaned black goats fed on Bacillus amyloliquefaciens fsznc-06 or Bacillus pumilus fsznc-09, but that of multiple or potentially pathogenic bacteria were lower, as compared with the control group. Tax4Fun analysis predicting the functional profiling of microbial communities showed that microbial communities in rumen or caecum were highly influential on metabolism and organism systems after feeding weanling goats with Bacillus amyloliquefaciens fsznc-06 or Bacillus pumilus fsznc-09. It was suggested that Bacillus amyloliquefaciens fsznc-06 and Bacillus pumilus fsznc-09 might be an auspicious antibiotic alternative to improve black goat growth and health by changing rumen and gut microbiota positively.

Effects of Thymol Supplementation on Goat Rumen Fermentation and Rumen Microbiota In Vitro.

This study was performed to explore the predominant responses of rumen microbiota with thymol supplementation as well as effective dose of thymol on rumen fermentation. Thymol at different concentrations, i.e., 0, 100 mg/L, 200 mg/L, and 400 mg/L (four groups × five replications) was applied for 24 h of fermentation in a rumen fluid incubation system. Illumina MiSeq sequencing was applied to investigate the ruminal microbes in addition to the examination of rumen fermentation. Thymol doses reached 200 mg/L and significantly decreased (p < 0.05) total gas production (TGP) and methane production; the production of total volatile fatty acids (VFA), propionate, and ammonia nitrogen, and the digestibility of dry matter and organic matter were apparently decreased (p < 0.05) when the thymol dose reached 400 mg/L. A thymol dose of 200 mg/L significantly affected (p < 0.05) the relative abundance of 14 genera of bacteria, three species of archaea, and two genera of protozoa. Network analysis showed that bacteria, archaea, and protozoa significantly correlated with methane production and VFA production. This study indicates an optimal dose of thymol at 200 mg/L to facilitate rumen fermentation, the critical roles of bacteria in rumen fermentation, and their interactions with the archaea and protozoa.

A Genomic Perspective on the Potential of Wild-Type Rumen Bacterium Enterobacter sp. LU1 as an Industrial Platform for Bio-Based Succinate Production.

Enterobacter sp. LU1, a wild-type bacterium originating from goat rumen, proved to be a potential succinic acid producer in previous studies. Here, the first complete genome of this strain was obtained and analyzed from a biotechnological perspective. A hybrid sequencing approach combining short (Illumina MiSeq) and long (ONT MinION) reads allowed us to obtain a single continuous chromosome 4,636,526 bp in size, with an average 55.6% GC content that lacked plasmids. A total of 4425 genes, including 4283 protein-coding genes, 25 ribosomal RNA (rRNA)-, 84 transfer RNA (tRNA)-, and 5 non-coding RNA (ncRNA)-encoding genes and 49 pseudogenes, were predicted. It has been shown that genes involved in transport and metabolism of carbohydrates and amino acids and the transcription process constitute the major group of genes, according to the Clusters of Orthologous Groups of proteins (COGs) database. The genetic ability of the LU1 strain to metabolize a wide range of industrially relevant carbon sources has been confirmed. The genome exploration indicated that Enterobacter sp. LU1 possesses all genes that encode the enzymes involved in the glycerol metabolism pathway. It has also been shown that succinate can be produced as an end product of fermentation via the reductive branch of the tricarboxylic acid cycle (TCA) and the glyoxylate pathway. The transport system involved in succinate excretion into the growth medium and the genes involved in the response to osmotic and oxidative stress have also been recognized. Furthermore, three intact prophage regions ~70.3 kb, ~20.9 kb, and ~49.8 kb in length, 45 genomic islands (GIs), and two clustered regularly interspaced short palindromic repeats (CRISPR) were recognized in the genome. Sequencing and genome analysis of Enterobacter sp. LU1 confirms many earlier results based on physiological experiments and provides insight into their genetic background. All of these findings illustrate that the LU1 strain has great potential to be an efficient platform for bio-based succinate production.

English

The study investigated the use of dried goat rumen contents (DGRC) on growth performance of broiler chickens. Rumen contents were obtained from goats immediately after slaughter during the wet season, sundried, milled and incorporated in experimental diets at levels of 0, 5 and 10%. The 0% DGRC diet was the control. The experimental diets were formulated on iso-caloric and iso-nitrogenous principles in line with the nutritional requirements for growing broiler birds. Experimental birds were first fed on a common starter broiler diet comprising of 21% CP and 3100 Kcal/kg feed from 0 to 21 days of age; thereafter the birds (21-42 days) were allotted to the experimental treatments in a completely randomized design (CRD) with three replications. A cage with 10 birds was the experimental unit. Experimental diets were offered in the morning and evening, water was provided ad lib. Feed offered and leftovers were weighed daily, and body weight changes were recorded on a weekly basis. The results showed that birds on the 5% diet had significantly (Linear, Quadratic P&lt;0.05) higher final body weights (FBWs), average daily gain (ADG) and better feed conversion ratio (FCR) compared to those o on diets with 0 and 10% DGRC. Apparent and ileal digestibility of nutrients was improved with incorporation of dried goat rumen contents in the diets. Sensory analysis showed that meat from birds on 5% DGRC diet had (P&lt;0.05) more oil content and softer meat across diets. It is concluded that, use of dried goat rumen contents (DGRC) in broiler diets improves growth performance and organoleptic qualities of broiler chicken meat. Key words: Digestibility, growth performance, rumen contents, sensory attributes. &nbsp

Selection of purification condition for recombinant endoglucanase originated from goat rumen bacteria in &lt;i&gt; Escherichia coli &lt;/i&gt;

Cellulase is an important enzyme that plays a role in cleaving β-1,4 glucoside on cellulose to release glucose, which is of economic value and can be applied in many different fields. The 1545 bp endoglucanase gene mined from goat rumen's bacterial metagenomic data was expressed in Escherichia coli Rosetta2. In this study, the recombinant endoglucanase was purified by his-tag affinity chromatography with differrent processes, such as using phosphate buffer with or without sodium cloride, pretreatment of samples with ammonium sulphate before supplying into affinity column, using various concentration of imidazole for washing... Finally the endoglucanse was sucessfully purified by his-tag affinity column using sodium chloride-free phosphate buffer of which 150 mM and 400 mM imidazole were used for washing and enzyme elution, respectively. The resulting enzyme showed its high purity of 99%. CMC plate assay confirmed that although less active than commercial cellulase (Sigma), the recombinant cellulase hydrolyzed CMC to form a clear zone (halo) around the well. The purified enzyme is capable of using as material for further analysis.

Diversity and Composition of Rumen Bacteria, Fungi, and Protozoa in Goats and Sheep Living in the Same High-Altitude Pasture.

Simple SummaryTibetan goats and sheep graze together but have different growth performances, immune responses, and feeding preferences in the Tibetan pasture. Rumen microbiota composed of bacteria, fungi, and protozoa are necessary for a healthy ruminant. Therefore, in this study, we comprehensively describe composition and diversity of bacteria, fungi, and protozoa in the high- altitude rumen. Compared with sheep, the bacteria that degrade crude protein and produce volatile fatty acids (VFA) were increased in the rumen of goats (Saccharofermentans and Lachnospiraceae_XPB1014) (p < 0.05). In addition, when compared with goats, the fungi and protozoa that degrade fiber were increased in rumen of sheep (Neocallimastigaceae and Metadinium) (p < 0.05). Furthermore, VFA were significantly increased in the rumen of goats compared with sheep (p < 0.05). The VFA level was consistent with differences in the microbiota composition in the rumen between goats and sheep. Under mixed grazing conditions, goats tend to select a high-crude protein diet that is good for growth, whereas sheep tend to select a high-lignin diet that is difficult to digest. Therefore, the different microbiota in the rumen of goats and sheep may be explained by dietary preference.Environmental adaptation of ruminants was highly related to microbiota in the rumen. To investigate the diversity and composition of bacteria, fungi, and protozoa in the rumen of high-altitude animals, amplicon gene sequencing was performed using rumen fluid samples derived from both Tibetan goats and sheep at the same pasture in a highland (altitude > 4800 m). Between these two species, the ruminal bacteria and fungi were significantly different at multiple taxonomic levels. The alpha diversity of bacteria was significantly high in goats (p < 0.05). One hundred and sixty-four and 29 Operational Taxonomy Units (OTUs) with significant differences were detected in bacteria and fungi, respectively. The abundance of bacteria, fungi, and protozoa in the rumen was characterized at multiple taxonomic levels, and we determined that Firmicutes, Bacteroidetes, Neocallimastigomycota, and Ciliophora were the most abundant bacteria, fungi, and protozoa. The family Neocallimastigaceae and the genus Metadinium had cellulose degradation capacity in the rumen with high abundance, thereby, suggesting that fungi and protozoa played an essential role in rumen fermentation. In addition, by comparing microbiota in the rumen of goats and sheep it was found, that the fiber-degrading fungi genus (Cyllamyces) was increased in the rumen of sheep (p < 0.05) whereas VFA-producing bacteria (Saccharofermentans and Lachnospiraceae_XPB1014) were increased in the rumen of goats (p < 0.05). Interestingly, in the rumen, no differences in protozoa were observed between goats and sheep (p > 0.05). Furthermore, when compared to sheep, level of acetic acid, propionic acid, and total volatile fatty acid (TVFA) were significantly increased in the rumen of goats (p < 0.05). Taken together, these results suggested microbiota in the rumen drive goats to better adapt to high-altitude grazing conditions.

Reducing protein content in the diet of growing goats: implications for nitrogen balance, intestinal nutrient digestion and absorption, and rumen microbiota

Reducing dietary CP content is an effective approach to reduce animal nitrogen excretion and save protein feed resources. However, it is not clear how reducing dietary CP content affects the nutrient digestion and absorption in the gut of ruminants, therefore it is difficult to accurately determine how much reduction in dietary CP content is appropriate. This study was conducted to investigate the effects of reduced dietary CP content on N balance, intestinal nutrient digestion and absorption, and rumen microbiota in growing goats. To determine N balance, 18 growing wether goats (25.0 ± 0.5 kg) were randomly assigned to one of three diets: 13.0% (control), 11.5% and 10.0% CP. Another 18 growing wether goats (25.0 ± 0.5 kg) were surgically fitted with ruminal, proximate duodenal, and terminal ileal fistulae and were randomly assigned to one of the three diets to investigate intestinal amino acid (AA) absorption and rumen microbiota. The results showed that fecal and urinary N excretion of goats fed diets containing 11.5% and 10.0% CP were lower than those of goats fed the control diet (P < 0.05). When compared with goats fed the control diet, N retention was decreased and apparent N digestibility in the entire gastrointestinal tract was increased in goats fed the 10% CP diet (P < 0.05). When compared with goats fed the control diet, the duodenal flow of lysine, tryptophan and phenylalanine was decreased in goats fed the 11.5% CP diet (P < 0.05) and that of lysine, methionine, tryptophan, phenylalanine, leucine, glutamic acid, tyrosine, essential AAs (EAAs) and total AAs (TAAs) was decreased in goats fed the 10.0% CP diet (P < 0.05). When compared with goats fed the control diet, the apparent absorption of TAAs in the small intestine was increased in goats fed the 11.5% CP diet (P < 0.05) and that of isoleucine, serine, cysteine, EAAs, non-essential AAs, and TAAs in the small intestine was increased in goats fed the 10.0% CP diet (P < 0.05). When compared with goats fed the control diet, the relative richness of Bacteroidetes and Fibrobacteres was increased and that of Proteobacteria and Synergistetes was decreased in the rumen of goats fed a diet with 10.0% CP. In conclusion, reducing dietary CP content reduced N excretion and increased nutrient utilization by improving rumen fermentation, enhancing nutrient digestion and absorption, and altering rumen microbiota in growing goats.

Effects of Piper sarmentosum extract supplementation on growth performances and rumen fermentation and microflora characteristics in goats.

This study was carried out to investigate the effect of diet Piper sarmentosum extract (PSE) on the growth performance, antioxidant properties, rumen fermentation and microflora in goats. Forty Hainan black goats with similar body weight were divided into four groups with supplementation of PSE in the concentrate at 0, 300, 600 and 1,200mg/kg, respectively, and fed for 56days. Results showed that average daily gain (ADG) was higher and feed intake/body gain (F/G) was lower in goats fed with PSE at 300mg/kg (p<.05). The activities of glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC) in the serum of goats differed among treatments and were greatest linearly when PSE was added at 1,200mg/kg (p<.05). The level ofmalondialdehyde (MDA) in the serum of goats differed among treatments and was lowest linearly when PSE was added at 1,200mg/kg (p<.05). The level of protozoal protein in the rumen of goats differed among treatments and was lowest linearly when PSE was added at 1,200mg/kg (p<.05). The concentrations of ruminal acetic acid and valeric acid and the ratio of acetate to propionate were reduced with PSE supplementation (p<.05). Protozoa, fungi, Ruminococcus flavefaciens and Fibrobacter succinogenes contents differed among treatments and were lowest linearly when PSE was added at 1,200mg/kg (p<.05). Thus, supplementation of PSE at 300-1,200mg/kg to goat concentrate is recommended for improving antioxidative ability and rumen efficiency and reducing protozoal content of goat.

Prevalence of Rumen and Reticulum Trematodes in The Goat Slaughtered at Pegirian Surabaya Slaughter House using Digestive Surgery Method

The purpose of this study was to determine the kind of trematodes which infected the rumen and reticulum of goat slaughtered at RPH Pegirian Surabaya using digestive surgery method, and determine the prevalence result. 36 samples of rumen and reticulum were collected from RPH Pegirian from September - November 2017 then using digestive surgery to obtain fresh adult worms, afterwards using the Semichen-Acetic Carmine staining method to makes the object easy to identifying. The results showed that species was found on goat was Paramphistomum cervi and Cotylophoron cotylophorum, in the Paramphistomum cervi are several characters wich is oral sucker, genital pore, uterus, testis, ventral sucker and in Cotylophoron cotylophorum contain oral sucker, branched oesophagus, uterus, vitelin glands, testes , posterior sucker. 3 rumen positive trematodosis with prevalence of 8.33% Chi-square statistic test (p&gt;0.05) showed no effect between goat species and Trematodosis prevalence, 20 kacang goat and 16 peranakan etawa goat found 2 positive kacang goat (10%) and 1 peranakan etawa goat positive (6.25%).