Affected by the extreme environment of high temperature and high pressure downhole, the mechanical behavior of the sealer rubber sleeve undergoes significant changes during service, leading to a sharp decline in the mechanical properties of the rubber material within the high-temperature environment. To address the issue of seal failure in the sealer rubber barrel under high-temperature loads, experimental investigations were conducted to analyze the compression properties of rubber materials at various temperatures. The constitutive parameters of the rubber were then fitted using the Reduce-Polynomial model. Subsequently, a finite element model was employed to study the sealing performance and mechanical response of the three-compartment rubber-tube structure sealer at different temperatures. The findings demonstrate that temperature fluctuations have a substantial impact on the sealing performance and bearing strength of the sealer. Consequently, this research holds crucial guiding significance for the structural design of the sealer rubber cylinder under high-temperature conditions.