The use of recycled rubber in various civil engineering applications has seen a considerable rise in recent years. The rubber-soil mixture can exhibit a higher energy absorption capacity than soil alone, which would reduce the energy applied to structures, the stresses, and shocks they undergo. Base isolation is the process of isolating the base of a structure to limit the effects of earthquakes, so that the seismic forces applied to the base of the bedrock cannot move with the same intensity towards the super structure.In this work, the beneficial effects of rubber mixtures (RSM) in mitigating earthquakes when these mixtures are inserted as layers in the soil profile are highlighted; the approach is based on a method of using worn tires for seismic protection applications for infrastructures subjected to seismic loads. It involves mixing particles from used tires with soil materials and placing the mixtures under the foundation of the building as geotechnical insulation systems for vibration absorption.The foundation of a four-storey building is implanted in a soil profile in which a layer of rubber-sand mixture (RSM) has been inserted; to perform the role of seismic isolation when subjected to seismic excitation.The investigation is focused on the variation in the thickness of the layer on the one hand (between 1m and 4m), and on the other hand on the position of this layer in the soil mass (top, middle and bottom of the profile).The peaks of horizontal accelerations recorded at the base and top of the structure show that the sand-rubber mixture has adequate and promising potential for their application as seismic isolation materials for low-rise buildings. Moreover, the thickness of the layer and its location are two very determining parameters in the improvement of the performances of these RSM mixtures, especially in terms of reduction of the acceleration peak recorded.