You have accessJournal of UrologyBladder Cancer: Basic Research (III)1 Apr 20131126 MICRO RNA 100 ROLE IN THE CONTROL OF GENES IN BLADDER CANCER CELL LINES Denis Morais, Sabrina Reis, Nayara Viana, Caio Moura, Nelson Dip, Manuel Florez, Miguel Srougi, and Katia Leite Denis MoraisDenis Morais São Paulo, Brazil More articles by this author , Sabrina ReisSabrina Reis São Paulo, Brazil More articles by this author , Nayara VianaNayara Viana São Paulo, Brazil More articles by this author , Caio MouraCaio Moura São Paulo, Brazil More articles by this author , Nelson DipNelson Dip São Paulo, Brazil More articles by this author , Manuel FlorezManuel Florez São Paulo, Brazil More articles by this author , Miguel SrougiMiguel Srougi São Paulo, Brazil More articles by this author , and Katia LeiteKatia Leite São Paulo, Brazil More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.741AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Introduction: Micro RNA (miRNA) are small non-coding RNA sequences that have large role in the control of gene expression by inhibiting protein translation or promoting the degradation of messenger RNA (mRNA) and is currently involved in various physiological and pathological cellular processes, including cancer where they can act as oncogene (oncomiR) or tumor suppressor gene (tsmiR). Recently, reduction in miR-100 expression has been correlated to low-grade, non-invasive bladder cancer (BCa), as an alternative to FGFR3 mutation and upregulation because FGFR3 is a supposed target of miR-100. Our aim in this study is to analyze the role of miR-100 in BCa cell lines in controlling the expression of some of its supposed target genes that could be important in BCa progression METHODS Methods: BCa cells lines RT4 and T24 were transfected with pre-miR-100 and their respective controls using Lipofectamine 2000. After 24 h of exposure, analysis of the expression level of target genes, THAP2, BAZ2A, mTOR, SMARCA5 and FGFR3 were analyzed by qRT-PCR. To analyze protein expression Western blot was performed after 48 h of RT4 and T24 miR-100 and control transfection. The analysis of protein expression was performed in the Alliance 4.7 equipment and software Alliance 16.06. The data shown related to gene expression are representative of three independent experiments. Statistical significance was calculated by two-tailed Student's t-test and p<0.05 was considered significant. Statistical analyses were performed using PRISM software. RESULTS Results: The expression levels of mRNA and proteins of RT4 and T24 cell lines after transfection with pre-miR100 are exposed in table 1. CONCLUSIONS Conclusion: We can affirm that miR-100 is negatively regulating the expression of mTOR, FGFR3, BAZ2A, SMARCA5 and THAP2 since there was a reduction in mRNA and/or protein expression after cell transfection with the miRNA. miR-100 may play an important role in BCa influencing these genes important in the control of cell proliferation and survival and chromosome instability. Cell line RT4 T24 Gene Gene expression p Reduction in protein expression (%) Gene expression p Reduction in protein expression (%) mTOR 0.467 0.0066 38.7 0.692 0.0234 57.1 FGFR3 0.794 0.2759 40.5 0.720 0.0996 47.0 THAP2 0.685 0.0533 29.8 0.757 0.075 99.7 SMARCA5 0.304 0.0070 49.5 0.608 0.0150 51.7 BAZ2A 0.488 0.0289 30.8 0.668 0.0804 27.9 © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e460 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Denis Morais São Paulo, Brazil More articles by this author Sabrina Reis São Paulo, Brazil More articles by this author Nayara Viana São Paulo, Brazil More articles by this author Caio Moura São Paulo, Brazil More articles by this author Nelson Dip São Paulo, Brazil More articles by this author Manuel Florez São Paulo, Brazil More articles by this author Miguel Srougi São Paulo, Brazil More articles by this author Katia Leite São Paulo, Brazil More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...