Rotavirus In Children Research Articles

Diarrhoea in north Karnataka: Rotavirus versus non-rotavirus

Background: As children with diarrhoea are often prescribed antibiotics, it was decided to study the causative organisms. Rotavirus is a major cause of diarrhoea and diarrhoea related mortality in most parts of India. Rotavirus vaccine is available but expensive. According to the World Health Organisation (WHO) the vaccine should be used in areas where diarrhoea related under five mortality is around 10%. The situation in north Karnataka is not known. Objectives : To find proportions of rotavirus and non-rotavirus diarrhoea in children of north Karnataka and study the key clinical features of rotavirus diarrhoea. Study design and settings : Prospective observational study conducted in patients from the paediatric ward and outpatient department in a tertiary care hospital. Method: Children 1 to 36 months old, presenting with acute diarrhoea from July 2014 to May 2016 and fulfilling selection criteria were included. Demographic and clinical details were noted. Stools were collected within 24 hours and subjected to routine and microscopic examination, test for presence of reducing substance, bacterial culture and Rapid ELISA test for rotavirus using antigen detection micro-well ELISA kit by Premere Rotaclone. Results: A total of 168 children was recruited . Children positive for rotavirus ELISA were labelled as having rotavirus diarrhoea and comprised 52 (31%) of total, including 17 with mixed infection and 15% were bacterial culture positive. Most common age group affected was 7 to 18 months (79%).Male female ratio was same in rotavirus and non-rotavirus diarrhoea. The clinical features defining rotavirus diarrhoea were fever, vomiting, watery stools, respiratory symptoms and perianal excoriations. Moderate and severe dehydration were common. There was no mortality. Conclusions : Around one third (31%) of diarrhoea cases were positive for rotavirus and 15% were of only bacterial aetiology. Key clinical features in rotavirus infection were fever, vomiting, watery stools, respiratory symptoms and perianal excoriations. Sri Lanka Journal of Child Health , 2018; 47 : 204-209

Rotavirus in diarrhoeic children under-five attending a tertiary care hospital in Mumbai, India

Background: Diarrhoeal diseases are a leading cause of morbidity and mortality among children requiring hospitalization in developing countries. Rotavirus is the most common cause of acute diarrhoea among under-five children. Etiological diagnosis of diarrhoea would enable appropriate management of patients while limiting the spread of drug resistant pathogens. This study was undertaken to determine presence of rotavirus and other diarrhoeal pathogens in under-five diarrhoeic children attending a tertiary care hospital, and the related clinical presentations.Methods: 120 stool samples of under-five children with acute diarrhoea, attending the OPD and indoor services of a tertiary care hospital, were studied over one year. Rotavirus antigen was detected using enzyme linked immunosorbent assay. Bacterial and parasitic pathogens were detected using standard microbiological techniques.Results: Out of 120 stool samples tested, 36 were positive for Rota virus antigen. Bacterial isolates included E. coli (25), Vibrio cholerae (12) and Aeromonas species (1). Parasites were observed in nine samples and multiple pathogens in nine.Conclusions: Rotavirus continues to be a major cause of childhood diarrhoea. As antibiotics have no role in the management of viral and parasitic diarrhoeas, etiological diagnosis is imperative for proper management of diarrhoea and prevention of indiscriminate use of antibiotics.

Prevalence of rotavirus and rapid changes in circulating rotavirus strains among children with acute diarrhea in China, 2009–2015

Rotavirus is a leading cause of morbidity and mortality in young children worldwide. In China, the universal immunization of children with the rotavirus vaccine has not been introduced, and the two globally distributed vaccines (RotaTeq and Rotarix) are not licensed in the country. We aim to determine the prevalence and strain diversity of rotavirus in children with diarrhea aged≤five years across China. Sentinel-based surveillance of acute diarrhea was conducted at 213 participating hospitals in China from January 1, 2009, through December 31, 2015. Group A rotavirus (RVA) was tested by using enzyme-linked immunosorbent assays, and G- and P-genotype of RVA were tested by RT-PCR methods. Of 33,616 children with diarrhea, 10,089 (30%) were positive for RVA; RVA-associated diarrhea was identified in 2247 (39.5%, n = 2247/5685) inpatients and 7842 (28.1%, n = 7842/27931) outpatients. Children living in low-middle-income regions suffered from the highest burden of rotavirus, with 40.7% of diarrhea cases attributed to rotavirus infection, followed by 31.3% in upper-middle-income and 11.2% in high-income regions. The majority of children (88.9%, n = 8976/10089) who tested positive for RVA were children aged≤2 years. The seasonal peak of RVA was in the winter. Among all 2533 RVA strains genotyped, five strain combinations, G9P[8], G3P[8], G1P[8], G2P[4] and G3P[4], contributed to 71.3% (1807/2533) of the RVA-associated diarrhea cases. The predominant strain of RVA has rapidly evolved from G3P[8] and G1P[8] to G9P[8] in the recent years, with the proportion of G9P[8] having increased remarkably from 3.4% in 2009 to 60.9% in 2015. The burden of diarrhea attributed to rotavirus is high in China, highlighting the potential value of vaccination. The rapid shift of RVA strains highlights the importance of conducting rotavirus surveillance to ensure that currently marketed vaccines provide protective efficacy against the circulating strains.

Therapeutic effect of phenyl vinyl sulfone and nitazoxanide on experimentally infected mice with cryptosporidiosis

Objective Evaluation of the therapeutic effect of phenyl vinyl sulfone (PVS) as cysteine protease inhibitors, nitazoxanide (NTZ), and combined therapy on Cryptosporidium parvum infection regarding the parasitological and histopathological parameters. Background Cryptosporidium spp. had been identified as the second most important diarrheal pathogen after rotavirus in young children. The immune status of the host plays a critical role. There is no reliable treatment for cryptosporidiosis, as the only approved drug NTZ provides no benefit for immunocompromised patients. Materials and methods A total of 180 female laboratory-bred Swiss albino mice were divided into two major divisions, immunocompetent and immunosuppressed, with the following groups for each one, respectively: negative control (I and VI), infected control (II and VII), infected treated with PVS (III and VIII), infected treated with NTZ (IV and IX), and infected treated combined (V and X). Stool examination for oocyst shedding was done at different days postinfection, and mice were killed at 18 and 30 days postinfection (groups A and B, respectively). Histopathological assessment of the ileum was done, and the endogenous developmental stages of the parasite were counted. Results Combined therapy in groups V and X resulted in the highest reduction in Cryptosporidium spp. oocysts shedding (P = 0.044 and Conclusion Combined therapy is more effective than either NTZ or PVS used alone. NTZ is still a better treatment option than PVS.

Detection and Genotyping of Viral Gastroenteritis in Hospitalized Children Below Five Years Old in Cairo, Egypt

: This work aimed to study the detection and genotyping of rotavirus, enteric adenovirus, and astrovirus in Egyptian hospitalized children below five years old, associated with non-bacterial diarrhea. In total, 119 fecal samples were obtained from the target population, admitted to Abu El-reesh hospital in Cairo, Egypt. Based on the findings, the detection rate of at least one viral infection was 36.7% in children below five years old, whereas, the overall detection rate of rotavirus, adenovirus, and coinfection was 31%, 6.7%, and 0.8%, respectively. No astrovirus infection was observed. Spring was the peak season for rotavirus and enteric adenovirus. The findings showed that higher rates of rotavirus (78%) and enteric adenovirus (100%) were identified in children less than two years of age. The dominant genotypes were G1P [8] (29.7%), G3P [8] (27%), and G1P [4] (18.9%) for rotaviruses and genotype 41 for enteric adenoviruses. Uncommon genotypes G1P [6] and G9P [8] were also detected in five (13.5%) and four (10.8%) samples, respectively. The present findings indicated that the high incidence of rotavirus and adenovirus in children below two years old. Thus, highlighting the necessity of vaccine development to reduce the incidence of acute viral gastroenteritis in Egypt.

Diarrhea among hospitalized children under five: A call for inclusion of rotavirus vaccine to the national immunization program in Indonesia

ContextRotavirus diarrhea is a common disease worldwide which mostly affects children under five years old. Rotavirus infection causes severe diarrhea and leads to substantial health care costs. In Indonesia the rotavirus vaccine has been available since 2011, however it has not been included into the National Immunization Program. This study aims to describe the proportion of rotavirus in children under 5 in Indonesia, the clinical characteristics of rotavirus infections, and the rotavirus strains circulating in the country during 2010–2015. MethodsChildren under five years of age with acute watery diarrhea were prospectively identified and enrolled through the active diarrhea surveillance system in 5 sites in four provinces in Indonesia during 2010–2015. The rotavirus specimens were tested using Enzyme Immunoassay. Bivariate logistic regression tests were performed to compare rotavirus positive and negative results with respect to the collected demographic and clinical variables. ResultsFrom January 2010 to December 2015, the average annual rotavirus prevalence among children hospitalized with acute watery diarrhea in four provinces in Indonesia was 47.5%. Rotavirus diarrhea occurred mostly in children under 2 years of age. Of all age groups, children aged 6–11 and 12–23 months had the highest prevalence of rotavirus diarrhea in all years (54.2% and 50.6%, respectively). This study found that the most prevalent of G and P genotypes were G1P8 in 2010 (63.2%), 2011 (64.1%) and 2012 (74.6%) and G3P8 in 2013 (49.7%), 2014 (82.5%) and 2015 (84.4%) ConclusionsThis study demonstrates that rotavirus is a major cause of diarrhea in hospitalized children in Indonesia. These findings highlight the need for inclusion of the rotavirus vaccine to the National Immunization Program in Indonesia.

Rotavirus and norovirus in children with severe diarrhea in Burkina Faso before rotavirus vaccine introduction.

Burkina Faso introduced rotavirus vaccine (RotaTeq) to the national immunization program in November 2013. This study describes the detection rates, clinical profiles, and molecular epidemiology of rotavirus and norovirus (NoV) infections among children <5 years hospitalized (n = 154) because of acute diarrhea in Ouagadougou, Burkina Faso, from December 2012 to November 2013, just before the start of vaccination. Overall, 44% and 23% of fecal samples were positive for rotavirus and NoV, respectively, most of them detected during the cold dry season (December-March). The predominant G/P combinations were G12P[8] (47%) and G6P[6] (30%). G2P[4] (n = 3), G12P[6] (n = 3), and G6P[8] (n = 1) werealso detected. Nearly all (94%) successfully genotyped NoV strains belonged to genotype GII.4. The predominance of rotavirus and NoV was noteworthy in the age group ≤6 months, with 67% rotavirus and 22% NoV, respectively. Vomiting was significantly more common among rotavirus-infected children. To conclude, this study shows high detection rates of both rotavirus and NoV in children with severe diarrhea in Burkina Faso just before the introduction of rotavirus group A vaccination. The results can be used for estimating the impact of rotavirus group A vaccination, which started in the end of 2013. Furthermore, this study shows that the G6P[6] rotavirus strains emerging in Burkina Faso in 2010 is now established as a regionally important genotype.

Rotavirus diarrhea in hospitalized children under 5 years of age in Vientiane, Lao PDR, 2009–2015

BackgroundDiarrhea is a leading cause of death in children <5 years worldwide, causing an estimated 215,000 deaths in 2013. This evaluation tracks the epidemiologic patterns and most common rotavirus genotypes among hospitalized children in this age group with acute gastroenteritis in Lao PDR. MethodsChildren <5 years in a central tertiary hospital in the capital city were prospectively enrolled into the surveillance platform during January 2009—December 2015. We collected information regarding clinical characteristics of enrolled children. Stool samples were obtained within 24 h of hospital admission and tested for rotavirus using rotavirus antigen detection enzyme immunoassay. Samples were sent to the regional reference laboratories in Australia and South Korea for genotyping. Bivariate analyses compared demographic and clinical characteristics between rotavirus positive and negative children using Chi-square statistical testing. Seasonality of rotavirus and annual genotype distribution are also described. ResultsWe enrolled 1853 children <5 years with acute gastroenteritis during the surveillance period and collected 1772 fecal specimens, 982 (55%) of which tested positive for rotavirus. A higher proportion of rotavirus acute gastroenteritis was observed among children 12–23 months of age as compared to rotavirus negative children in the same age group, 41% vs 36%. Eighty-six percent of rotavirus positive children experienced vomiting, as compared to 65% of rotavirus negative children. Eighty-five percent (n = 830/982) of rotavirus positive specimens occurred during the dry season (January-April). The most common genotypes identified were G1, G2, G3 and P8 and P4. The most prevalent combined genotype differed annually during the surveillance period. ConclusionSurveillance continues to be important in documenting the burden of rotavirus in children <5 years in Lao PDR as well as providing a baseline for determining the impact of rotavirus vaccine once it is introduced into Lao PDR’s national immunization schedule.

Genotypic characterization of rotavirus in children under 5 years circulating in C\xf4te D\u2019Ivoire from 2010 to 2013

BackgroundRotavirus infection is the most common cause of severe gastroenteritis in children under five years of age in both developed and developing countries. The World Health Organisation (WHO) recommends the surveillance of rotavirus strains prior to vaccine introduction in all applicable countries. The objective of this study was to describe the epidemiological characteristics as well as to determine the circulating genotypes of rotaviruses in Côte d’Ivoire prior to vaccine introduction.MethodsThe study included children under five years of age who met the inclusion criteria after informed consent had been sort from their parents or guardians. Rotavirus VP6 antigens were detected for each stool sample using Enzyme Immunoassay (EIA). Genotyping of positive EIA samples was performed by reverse-transcriptase-PCR (RT-PCR) assays.ResultsA total of 684 children were recruited. Children aged between 6 and 11 months were the most represented with 34%. Rotavirus VP6 antigens were found in 27.1% (186/684) of samples tested. Commonly detected G genotypes included G12 (46.6% (82/176) and G1 (13.1% (23/176) whilst P[8] (49.8% (91/183) was the most predominant P genotype. Rotavirus G12P[8] was the most predominant strain circulating in Côte d’Ivoire within the period of study and constituted 26.6% of all strains detected.ConclusionThe monitoring of circulating strains will help guide decision-makers in the choice of vaccine. Genotypic variability of circulating rotavirus strains over the years implies there is a need for continuous rotavirus strain surveillance even after vaccine introduction.

Increasing predominance of G8P[8] species A rotaviruses in children admitted to hospital with acute gastroenteritis in Thailand, 2010-2013.

Rotavirus A is a well-known etiological cause of acute gastroenteritis in infants and young children worldwide. In this study, we investigated the prevalence and distribution of RVA genotypes circulating in children with acute gastroenteritis in Thailand from 2010 to 2013. A total of 1,032 fecal specimens were collected from children with an age range from neonatal to 15 years of age and tested for RVA by RT-PCR. Of these, 184 (17.8%) were positive for RVA. The highest detection rate of RVA was found in children aged between 12 and 24 months. The G1P[8] genotype was identified as the most dominant genotype (57.6%), followed by G2P[4] (12.5%), G8P[8] (10.4%), G9P[8] (7.1%), G3P[8] (4.9%), G1P[4] (2.2%), G2P[8] (1.7%), and mixed-infections of G1 and G3 in combination with P[8] (0.5%). In addition, the uncommon human rotavirus strains G4P[6] (1.1%), G9P[19] (0.5%), G12P[4] (0.5%), and G12P[6] (0.5%) were also detected in this study. Interestingly, the unusual G8P[8] strains were detected at a relatively high frequency, and phylogenetic analysis revealed that these G8 strains were genetically closely related to bovine and bovine-like human G8 rotavirus strains reported previously from Thailand, Japan, Vietnam, India and Taiwan. These G8P[8] strains displayed the DS-1-like genotype constellation of G8-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2 (in the order VP7-VP4-VP6-VP1-VP2-VP3-NSP1-NSP2-NSP3-NSP4-NSP5/6, respectively). Overall, the data indicated a high degree of diversity of RVA genotypes, with the emergence of several uncommon RVA strains in children with acute gastroenteritis in Thailand.

Species C Rotaviruses in Children with Diarrhea in India, 2010-2013: A Potentially Neglected Cause of Acute Gastroenteritis.

All over the world, children and adults are severely affected by acute gastroenteritis, caused by one of the emerging enteric pathogens, rotavirus C (RVC). At present, no extensive surveillance program is running for RVC in India, and its prevalence is largely unknown except cases of local outbreaks. Here, we intended to detect the presence of RVC in diarrheic children visiting or admitted to hospitals in Haldwani (state of Uttarakhand, India), a city located in the foothills of the Himalayas. During 2010–2013, we screened 119 samples for RVC by an RVC VP6 gene-specific RT-PCR. Of these, 38 (31.93%) were found positive, which is higher than the incidence rates reported so far from India. The phylogenetic analysis of the derived nucleotide sequences from one of the human RVC (HuRVC) isolates, designated as HuRVC/H28/2013/India, showed that the study isolate belongs to genotype I2, P2 and E2 for RVC structural genes 6 and 4 (VP6, and VP4) and non-structural gene 4 (NSP4), respectively. Furthermore, the VP6 gene of HuRVC/H28/2013/India shows the highest similarity to a recently-reported human-like porcine RVC (PoRVC/ASM140/2013/India, KT932963) from India suggesting zoonotic transmission. We also report a full-length NSP4 gene sequence of human RVC from India. Under the One-health platforms there is a need to launch combined human and animal RVC surveillance programs for a better understanding of the epidemiology of RVC infections and for implementing control strategies.

Severity and treatment level of acute gastroenteritis with rotavirus in children under 5 years in INDONESIA

Severity and treatment level of acute gastroenteritis with rotavirus in children under 5 years in INDONESIA

Prevalence of Rotavirus and Adenovirus in children with acute gastroenteritis: a tertiary care hospital data

Introduction: The aim of this study was to determine the prevalence of rotavirus and adenovirus in patients who applied with the complaint of acute gastroenteritis to the emergency department of our tertiary health care provider hospitals. Methods: 1712 patients with the complaint of acute gastroenteritis were included in the study and the study was done retrospectively from patient records.Results: Stool (faeces) samples have been sent from 1712 patients who applied to emergency services with the complaint of acute gastroenteritis for 3 years. Of these, 17.5% (n=301) rotavirus antigen and 3.5% (n=61) adenovirus antigen were detected as positive. It was observed that 56% (n=203) of the positive cases were male and 44% (n=159) were female. The rotavirus antigen was statistically significantly higher in the 0-4 age group than the other age groups. Conclusion: Acute gastroenteritis is an important health problem in children. Especially, the frequency of rotavirus was observed to be higher in children under 4 years of age and in this age group patients the use of rotavirus rapid diagnostic tests rather than viral culture methods may be helpful to early diagnosis thus to reduce unnecessary antibiotic use.

Sentinel surveillance for rotavirus in children

BackgroundRotavirus is the leading cause of severe acute gastroenteritis (AGE) in children <5 years of age in Myanmar. The purpose of this analysis is to report from the sentinel surveillance system for rotavirus gastroenteritis (RVGE), which collects information on the epidemiology and circulating genotypes to assess the disease burden and support vaccine introduction in Myanmar. MethodsProspective, active surveillance for RVGE-associated hospitalizations was conducted during 2009 –2014 at Yangon Children’s Hospital. Stool samples collected from children <5 years of age admitted for AGE were screened for rotavirus antigen by ELISA (ProSpecT™ Rotavirus, OXOID-UK). G and P genotyping was performed by reverse transcription polymerase chain reaction. ResultsOverall, 1860/3724 (49.9%) of stool samples tested positive for rotavirus, ranging from 42-56% of hospitalized AGE cases each year. RVGE was predominant in the 6–11 months age group 889/1860 (47.8%) as compared with 12–23 months 633/1860 (34.0%), 0–5 months 226/1860 (12.2 %) and 24–59 months 112/1860 (6.0%). RVGE occurred in a seasonal cycle with peak occurrence in the cold and dry months (November to February), accounting for 65.3% (1151/1763) among enrolled AGE cases. Vomiting (84.1% Vs 67.9%; P < .01), fever (84.5% Vs 75.6%; P < .01) and dehydration (78% Vs 69%; P < .01) were more frequently observed in RVGE than non-RVGE.Genotyping revealed that G1P[8] was predominant from January to June 2009, G12P[8] was predominant throughout 2009–2012 which was replaced in 2012–2013 by G2P[4] and changed again to G1P[8] in 2013–2014 and G9P[8] in late 2014. ConclusionsRotavirus is accounting for approximately half of AGE-associated hospitalizations among children <5 years of age in Myanmar. There is immense diversity of rotavirus strains similar to that reported previously for other countries in the region. Information gained from this surveillance system highlights consideration of rotavirus vaccine introduction into this target population.

Molecular Epidemiology of Rotavirus Strains in Under Five Children.

To determine the prevalence and genotype distribution of rotaviruses in children presenting to authors' hospital in Middle Black Sea region of Turkey. The results may supply important information about vaccine studies in Turkey. Rotavirus antigen was detected by latex agglutination test and rotavirus RNA was detected by RT-PCR test. On the other hand, rotavirus positive samples were genotyped by semi-nested multiplex polymerase chain reaction. The highest rate of rotavirus positivity (46.9%) was observed among children in the 13 to 24 mo age group. All the positive-strains were in G1-4, G8-9, P [4], P [8], and P [9] genotypes. The most common G and P combination in present study was G9P[8] (n=24, 28.9%). The present results indicated that the most prevalent genotypes were G1, G9, P8, G9P[8] and G1P[8] in authors' region. Rotavirus vaccines used in this region must include mainly these genotypes.

Emergence of G9P[8] rotaviruses in children with acute gastroenteritis in Thailand, 2015‐2016

Human group A rotavirus is a major contagious virus causing gastroenteritis in children. Molecular epidemiological study of group A rotavirus infections in hospitalized children was performed by multiplex RT-PCR during 2015-2016 in Chiang Rai, Thailand. G- and P-genotypes of positive rotavirus samples were further analyzed by one-step and two-step multiplex RT-PCR methods. Among 270 fecal specimens tested, rotavirus was the most prevalent (33.7%), followed by norovirus GII (4.1%), adenovirus (3%), and astrovirus (1.5%). Infection was common in patients aged 12-23 months (45.1%) and occurred mostly in children under 3 years of age (85.7%). The highest peak was in a hot month, March (64.8%). G9P[8] emerged as the most predominant (79.1%), followed by G3P[8] (13.2%), G1P[8] (3.3%), and mixed G-types (4.4%). Interestingly, Chiang Rai G9 strains were clustered within a distinct lineage VII including G9 recently reported since 2010-2015. G9-VII also contained four to five unique amino acid substitutions in the VP7 proteins compared with those of the G9 candidate vaccine strain RVA/Human-tc/IND/116E/1985/G9P[11] and the prototype RVA/Human-wt/USA/WI61/1983/G9P[8], defining the G9-VII as a novel variant. G3 strains were closely related to the "new G3P[8] reassortant variant" with an equine-like VP7 gene that emerged in several countries. This study contributes to the understanding of the genetic diversity, providing scientific support for future vaccine strategies to reduce the morbidity and mortality.

The Global Burden of Rotavirus Diarrheal Diseases: Results from the Global Burden of Diseases Study 2016

BackgroundMore than 1,300,000 deaths were attributable to diarrhea in 2015, with more than 400,000 of these deaths from children under 5 years of age. The Global Burden of Disease Study 2016 (GBD2016), an ongoing effort to measure global epidemiological trends, estimates diarrhea disease burden and the burden attributable to rotavirus and other enteric pathogens.MethodsDiarrhea deaths are estimated using a suite of prediction models for all ages, both sexes, and for all countries and some subnational geographic areas from 1980 to 2016 using an ensemble modeling tool called CODEm. To estimate the burden of rotavirus, we calculated a population attributable fraction using a counter-factual approach by modeling the proportion of diarrheal cases that are positive for rotavirus and applying odds ratios describing the odds of diarrhea given rotavirus detection.ResultsIn 2016, rotavirus was the leading cause of diarrhea mortality in children under 5 years old, responsible for 29.5% of diarrhea deaths in this age group (149,200 deaths, 95% Uncertainty Interval (UI): 119,200–189,400), and responsible for 15.3% of diarrhea deaths among all ages (202,300 deaths, 95% UI: 165,800–246,400). The population attributable fraction of diarrhea mortality due to rotavirus is generally stable across geographic regions. The global attributable fraction of rotavirus decreased by 24.2% (95% UI: 16.7–31.6%) between 2005 and 2016ConclusionThe global deaths attributable to rotavirus in children under 5 is substantial and the burden in older children and adults may be unrecognized. GBD 2016 estimates describe epidemiological trends for rotavirus diarrhea and will inform evidence-based Public Health policy, to reduce the global burden of rotavirus. Our findings call for acceleration of delivery existing rotavirus vaccines and development of more affordable options for Low and Middle Income countries.Disclosures All authors: No reported disclosures.

Impact of rotavirus vaccination on rotavirus and all-cause gastroenteritis in peri-urban Kenyan children.

A monovalent rotavirus vaccine (RV1) was introduced into the National Immunization Program in Kenya in July 2014. We examined the impact of the vaccine on hospitalization for all-cause acute gastroenteritis (AGE) and rotavirus-specific AGE and strain distribution at a large referral hospital which serves a predominantly peri-urban population in Central Kenya. Data on rotavirus AGE and strain distribution were derived from ongoing hospital-based AGE surveillance. Hospital administrative data were used to compare trends in all-cause AGE. Pre-vaccine (July 2009-June 2014) and post-vaccine (July 2014-June 2016) periods were compared for changes in hospitalization for all-cause AGE and rotavirus AGE and strain distribution. Following the vaccine introduction, the proportion of children aged <5years hospitalized for rotavirus declined by 30% (95% CI: 19-45%) in the first year and 64% (95% CI: 49-77%) in the second year. Reductions in rotavirus positivity were most pronounced among the vaccine-eligible group (<12months) in the first year post-vaccination at 42% (95% CI: 28-56%). Greater reductions of 67% (95% CI: 51-79%) were seen in the second year in the 12-23months age group. Similarly, hospitalizations for all-cause AGE among children <5years of age decreased by 31% (95% CI: 24-40%) in the first year and 58% (95% CI: 49-67%) in the second year of vaccine introduction. Seasonal peaks of rotavirus and all-cause AGE were reduced substantially. There was an increased detection of G2P[4], G3P[6] and G3P[8], which coincided temporally with the timing of the vaccine introduction. Thus, introducing the rotavirus vaccine into the routine immunization program in Kenya has resulted in a notable decline in rotavirus and all-cause AGE hospitalizations in Central Kenya. This provides early evidence for public health policy makers in Kenya to support the sustained use of the rotavirus vaccine in routine immunizations.

Çorum’da Akut Gastroenteritli Çocuklarda Rotavirüs ve Adenovirüs Saptanma Sıklığı

Çorum’da Akut Gastroenteritli Çocuklarda Rotavirüs ve Adenovirüs Saptanma Sıklığı

Study of Incidence of Rota Virus in Children under 5 Years of Age with Acute Diarrhoea with Special Reference to Characterization of Rota Virus

Study of Incidence of Rota Virus in Children under 5 Years of Age with Acute Diarrhoea with Special Reference to Characterization of Rota Virus