Implementing the sentinel surveillance program of rotavirus infection in infants beginning with the year 2008 in the Republic of Moldova has proven the high rate of this infection (40%), an argument for recommending the children’s immunization against rotavirus within the National Immunization Program. Acute gastroenteritis is one of the most prominent actual health problems in infants, rotaviral infection being a frequent cause of dehydration in infants and small children. Aim. The assessment of clinical-evolutive and epidemiological features, with treatment optimization of rotavirus family infection in nonvaccinated and vaccinated children. Materials and method. There were included in the trial infants with acute diarrhea, from the sentinel surveillance (2012-2016) of the Pediatrics Department from the IMSP Clinical Hospital for Children No. 1. There were examined 193 patients with acute gastroenteritis, included by a standard case definition. The biologic material was examined with last-generation tests for rotavirus infection, using ELISA and genotype highlighting by PCR genic amplification. Results. The rotavirus infection was confirmed in 193 infants, from whom 121 were not vaccinated against rotavirus and 72 were immunized. In all patients, the disease began with toxic syndrome and dehydration in 100% of nonvaccinated cases, in comparison with vaccinated children – 82%. Conclusions. This article reflects the evolution of genotype characteristics of rotavirus family and of the clinical-paraclinical particularities of infection in infants, being of major importance in the context of implementing the immunization against rotavirus within the National Immunization Program in our country.