Detection Of Rotavirus In Stool Specimens Research Articles

Development and preliminary evaluation of a latex agglutination test using a monoclonal antibody for rotavirus detection in stool specimens

Resume Nous avons mis au point un test d’agglutination au latex utilisant un anticorps monoclonal pour la detection rapide des rotavirus dans les selles. L’anticorps monoclonal utilise a ete selectionne pour sa grande affinite et sa structure physico-chimique particuliere apres avoir ete couple aux billes du latex. Les qualites de cet anticorps conferent a ce test d’agglutination de nombreux avantages sur les tests d’agglutination utilisant des immunserums classiques: (1) tres grande sensibilite, (2) une agglutination plus intense et apparaissant plus rapidement et (3) une absence d’agglutination non specifique.

Development and evaluation of a rapid one-step elisa for rotavirus detection in stool specimens using only monoclonal antibodies

Summary We developed 3 monoclonal antibodies against the common antigen rotavirus which recognize two non-overlapping antigenic sites. These antibodies were selected for their high-affinity to establish an assay with a sensitivity towards the antigen comparable to that of conventional assays with polyclonal antisera. With these particular antibodies, it was possible to carry out an assay with a single 30-min incubation step, achieving a degree of sensitivity almost the same as the Enzygnost-Rotavirus when these methods were tested on our reference strains, 357 unselected stools specimens from children and 41 from neonates. Such a monoclonal ELISA in a one-step procedure gives the advantage of both speed and simplicity and would be applicable to large-scale screening.