Rotational Echo Double Resonance Research Articles

Surface Structure of Lecithin-Capped Cesium Lead Halide Perovskite Nanocrystals Using Solid-State and Dynamic Nuclear Polarization NMR Spectroscopy.

Inorganic colloidal cesium lead halide perovskite nanocrystals (NCs) encapsulated by surface capping ligands exhibit tremendous potential in optoelectronic applications, with their surface structure playing a pivotal role in enhancing their photophysical properties. Soy lecithin, a tightly binding zwitterionic surface-capping ligand, has recently facilitated the high-yield synthesis of stable ultraconcentrated and ultradilute colloids of CsPbX3 NCs, unlocking a myriad of potential device applications. However, the atomic-level understanding of the ligand-terminated surface structure remains uncertain. Herein, we use a versatile solid-state nuclear magnetic resonance (NMR) spectroscopic approach, in combination with dynamic nuclear polarization (DNP) and atomistic molecular dynamics (MD) simulations, to explore the effect of lecithin on the core-to-surface structures of CsPbX3 (X = Cl or Br) perovskites, sized from micron to nanoscale. Surface-selective (cross-polarization, CP) solid-state and DNP NMR (133Cs and 207Pb) methods were used to differentiate the unique surface and core chemical environments, while the head-groups {trimethylammonium [-N(CH3)3+] and phosphate (-PO4-)} of lecithin were assigned via 1H, 13C, and 31P NMR spectroscopy. A direct approach to determining the surface structure by capitalizing on the unique heteronuclear dipolar couplings between the lecithin ligand (1H and 31P) and the surface of the CsPbCl3 NCs (133Cs and 207Pb) is demonstrated. The 1H-133Cs heteronuclear correlation (HETCOR) DNP NMR indicates an abundance of Cs on the NC surface and an intimate proximity of the -N(CH3)3+ groups to the surface and subsurface 133Cs atoms, supported by 1H{133Cs} rotational-echo double-resonance (REDOR) NMR spectroscopy. Moreover, the 1H-31P{207Pb} CP REDOR dephasing curve provides average internuclear distance information that allows assessment of -PO4- groups binding to the subsurface Pb atoms. Atomistic MD simulations of ligand-capped CsPbCl3 surfaces aid in the interpretation of this information and suggest that ligand -N(CH3)3+ and -PO4- head-groups substitute Cs+ and Cl- ions, respectively, at the CsCl-terminated surface of the NCs. These detailed atomistic insights into surface structures can further guide the engineering of various relevant surface-capping zwitterionic ligands for diverse metal halide perovskite NCs.

Structure of Spider Silk Studied with Solid‐State NMR

AbstractSpider dragline silks exhibit remarkable mechanical properties, combining both high strength and toughness. These unique characteristics arise from the intricate structure of the silk, which requires atomic‐level information to understand its origins. 13C solid‐state NMR provides this detailed structural insight into spider dragline silk. In this review, 13C CP/MAS, 13C DD/MAS and 13C INEPT NMR spectroscopies are employed to reveal the structure of spider dragline silks together with 13C conformation‐dependent chemical shifts, 2D spin‐diffusion NMR, rotational echo double resonance, dipolar‐assisted rotational resonance, and angle‐dependent NMR. The primary structure of major ampullate of spider dragline silk consists of repeated polyalanine and a glycine‐rich regions. By analyzing the 13C conformation‐dependent chemical shifts and utilizing several solid‐state NMR techniques, it has been proposed that the glycine‐rich region primarily adopts a random coil conformation, including partially β‐sheet and β‐turn structures. This contradicts the previously suggested 31 helix conformation. On the other hand, the polyalanine region exhibits an antiparallel β‐sheet structure with staggered packing arrangements. Additionally, solid‐state NMR has also revealed the structure of fragelliform spider silk. These findings contribute to the understanding of the remarkable properties of spider dragline silks and provide insights into its atomic‐level architecture.

Parallel β-Sheet Structure and Structural Heterogeneity Detected within Q11 Self-Assembling Peptide Nanofibers.

Q11 peptide nanofibers are used as a biomaterial for applications such as antigen presentation and tissue engineering, yet detailed knowledge of molecular-level structure has not been reported. The Q11 peptide sequence was designed using heuristics-based patterning of hydrophobic and polar amino acids with oppositely charged amino acids placed at opposite ends of the sequence to promote antiparallel β-sheet formation. In this work, we employed solid-state nuclear magnetic resonance spectroscopy (NMR) to evaluate whether the molecular organization within Q11 self-assembled peptide nanofibers is consistent with the expectations of the peptide designers. We discovered that Q11 forms a distribution of molecular structures. NMR data from two-dimensional (2D) 13C-13C dipolar-assisted rotational resonance indicate that the K3 and E9 residues between Q11 β-strands are spatially proximate (within ∼0.6 nm). Frequency-selective rotational echo double resonance (fsREDOR) on K3 Nζ and E9 Cδ-labeled sites showed that approximately 9% of the sites are close enough for salt bridge formation to occur. Surprisingly, dipolar recoupling measurements revealed that Q11 peptides do not assemble into antiparallel β-sheets as expected, and structural analysis using Fourier-transform infrared spectroscopy and 2D NMR alone can be misleading. 13C PITHIRDS-CT dipolar recoupling measurements showed that the most abundant structure consists of parallel β-sheets, in contrast to the expected antiparallel β-sheet structure. Structural heterogeneity was detected from 15N{13C} REDOR measurements, with approximately 22% of β-strands having antiparallel nearest neighbors. We cannot propose a complete structural model of Q11 nanofibers because of the complexity involved when examining structurally heterogeneous samples using NMR. Altogether, our results show that while heuristics-based patterning is effective in promoting β-sheet formation, designing a peptide sequence to form a targeted β-strand arrangement remains challenging.

Experimental aspects of 14N overtone RESPDOR solid-state NMR spectroscopy under MAS beyond 60 kHz

Nitrogen-14 (14N) overtone (OT) spectroscopy under fast magic angle spinning (MAS) conditions (>60 kHz) has emerged as a powerful technique for observing correlations and distances between 14N and 1H, owing to the absence of the first-order quadrupolar broadenings. In addition, 14NOT allows selective manipulation of 14N nuclei for each site. Despite extensive theoretical and experimental studies, the spin dynamics of 14NOT remains under debate. In this study, we conducted experimental investigations to assess the spin dynamics of 14NOT using the rotational-echo saturation-pulse double-resonance (RESPDOR) sequence, which monitors population transfer induced by a14NOT pulse. The 14NOT spin dynamics is well represented by a model of a two-energy-level system. Unlike spin-1/2, the maximum excitation efficiency of 14NOT coherences of powdered solids, denoted by p, depends on the radiofrequency field (rf-field) strength due to orientation dependence of effective nutation fields even when pulse lengths are optimized. It is also found that the p factor, contributing to the 14NOT spin dynamics, is nearly independent of the B0 field. Consequently, the filtering efficiency of RESPDOR experiments exhibits negligible dependence on B0 when the 14NOT pulse length is optimized. The study also identifies the optimal experimental conditions for 14NOT/1H RESPDOR correlation experiments.

Surface Passivation by Embedment of Polyphosphate Inhibits the Aragonite-to-Calcite Thermodynamic Pump.

We monitored the conversion of aragonite to calcite in water by comparing single and mixed polymorph suspensions. We demonstrate that the enhanced aragonite-to-calcite conversion in mixed polymorph suspensions is dramatically inhibited by adding polyphosphate (sodium hexametaphosphate). 13C and 31P solid-state magic angle spinning (MAS) NMR and attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectra allow us to follow quantitatively these effects as imparted by the dissolution-recrystallization processes. 31P{13C} and 13C{31P} rotational echo double resonance (REDOR)NMR experiments reveal coprecipitated phosphate that is embedded only within the surfaces of both polymorphs during the initial dissolution and recrystallization processes, causing passivation that arrests phase conversion.

Three Decades of REDOR in Protein Science: A Solid-State NMR Technique for Distance Measurement and Spectral Editing.

Solid-state NMR (ss-NMR) is a powerful tool to investigate noncrystallizable, poorly soluble molecular systems, such as membrane proteins, amyloids, and cell walls, in environments that closely resemble their physical sites of action. Rotational-echo double resonance (REDOR) is an ss-NMR methodology, which by reintroducing heteronuclear dipolar coupling under magic angle spinning conditions provides intramolecular and intermolecular distance restraints at the atomic level. In addition, REDOR can be exploited as a selection tool to filter spectra based on dipolar couplings. Used extensively as a spectroscopic ruler between isolated spins in site-specifically labeled systems and more recently as a building block in multidimensional ss-NMR pulse sequences allowing the simultaneous measurement of multiple distances, REDOR yields atomic-scale information on the structure and interaction of proteins. By extending REDOR to the determination of 1H-X dipolar couplings in recent years, the limit of measurable distances has reached ~15-20 Å, making it an attractive method of choice for the study of complex biomolecular assemblies. Following a methodological introduction including the most recent implementations, examples are discussed to illustrate the versatility of REDOR in the study of biological systems.

Measuring Dipolar Order Parameters in Nondeuterated Proteins Using Solid-State NMR at the Magic-Angle-Spinning Frequency of 100 kHz

Proteins are dynamic molecules, relying on conformational changes to carry out function. Measurement of these conformational changes can provide insight into how function is achieved. For proteins in the solid state, this can be done by measuring the decrease in the strength of anisotropic interactions due to motion-induced fluctuations. The measurement of one-bond heteronuclear dipole-dipole coupling at magic-angle-spinning (MAS) frequencies >60 kHz is ideal for this purpose. However, rotational-echo double resonance (REDOR), an otherwise gold-standard technique for the quantitative measurement of these couplings, is difficult to implement under these conditions, especially in nondeuterated samples. We present here a combination of strategies based on REDOR variants ϵ-REDOR and DEDOR (deferred REDOR) and simultaneously measure residue-specific 15N-1H and 13Cα-1Hα dipole-dipole couplings in nondeuterated systems at the MAS frequency of 100 kHz. These strategies open up avenues to access dipolar order parameters in a variety of systems at the increasingly fast MAS frequencies that are now available.

Zwitterionic or Not? Fast and Reliable Structure Determination by Combining Crystal Structure Prediction and Solid-State NMR.

When it comes to crystal structure determination, computational approaches such as Crystal Structure Prediction (CSP) have gained more and more attention since they offer some insight on how atoms and molecules are packed in the solid state, starting from only very basic information without diffraction data. Furthermore, it is well known that the coupling of CSP with solid-state NMR (SSNMR) greatly enhances the performance and the accuracy of the predictive method, leading to the so-called CSP-NMR crystallography (CSP-NMRX). In this paper, we present the successful application of CSP-NMRX to determine the crystal structure of three structural isomers of pyridine dicarboxylic acid, namely quinolinic, dipicolinic and dinicotinic acids, which can be in a zwitterionic form, or not, in the solid state. In a first step, mono- and bidimensional SSNMR spectra, i.e., 1H Magic-Angle Spinning (MAS), 13C and 15N Cross Polarisation Magic-Angle Spinning (CPMAS), 1H Double Quantum (DQ) MAS, 1H-13C HETeronuclear CORrelation (HETCOR), were used to determine the correct molecular structure (i.e., zwitterionic or not) and the local molecular arrangement; at the end, the RMSEs between experimental and computed 1H and 13C chemical shifts allowed the selection of the correct predicted structure for each system. Interestingly, while quinolinic and dipicolinic acids are zwitterionic and non-zwitterionic, respectively, in the solid state, dinicotinic acid exhibits in its crystal structure a "zwitterionic-non-zwitterionic continuum state" in which the proton is shared between the carboxylic moiety and the pyridinic nitrogen. Very refined SSNMR experiments were carried out, i.e., 14N-1H Phase-Modulated (PM) pulse and Rotational-Echo Saturation-Pulse Double-Resonance (RESPDOR), to provide an accurate N-H distance value confirming the hybrid nature of the molecule. The CSP-NMRX method showed a remarkable match between the selected structures and the experimental ones. The correct molecular input provided by SSNMR reduced the number of CSP calculations to be performed, leading to different predicted structures, while RMSEs provided an independent parameter with respect to the computed energy for the selection of the best candidate.

Spatial Proximities between Brønsted Acid Sites, AlOH Groups, and Residual NH4+ Cations in Zeolites Mordenite and Ferrierite

The interaction between Brønsted acid sites (BAS), AlOH groups, and residual NH4+ cations in zeolites mordenite and ferrierite was investigated by solid-state 1H magic-angle spinning and 1H–27Al rotational-echo adiabatic-passage double-resonance (REAPDOR) nuclear magnetic resonance (NMR) techniques in combination with density functional theory (DFT) cluster calculations. Higher temperatures are needed for the full elimination of NH4+ (853 K instead of 723 K compared to other high-silica zeolites). NMR data reveal that residual NH4+ cations are primarily in the vicinity of BAS and other NH4+ in the case of mordenite and AlOH groups in ferrierite materials. DFT calculations for a variety of cluster models with BAS and NH4+ ions embedded in their zeolite environment rationalize the experimental data. It is suggested that NH4+ ions are preferably stabilized within 8-ring pores, explaining the higher temperature required to decompose them.

Resolving the Chemical Formula of Nesquehonite via NMR Crystallography, DFT Computation, and Complementary Neutron Diffraction.

Nesquehonite is a magnesium carbonate mineral relevant to carbon sequestration envisioned for carbon capture and storage of CO2 . Its chemical formula remains controversial today, assigned as either a hydrated magnesium carbonate [MgCO3 ⋅ 3H2 O], or a hydroxy- hydrated- magnesium bicarbonate [Mg(HCO3 )OH ⋅ 2H2 O]. The resolution of this controversy is central to understanding this material's thermodynamic, phase, and chemical behavior. In an NMR crystallography study, using rotational-echo double-resonance 13 C{1 H} (REDOR), 13 C-1 H distances are determined with precision, and the combination of 13 C static NMR lineshapes and density functional theory (DFT) calculations are used to model different H atomic coordinates. [MgCO3 ⋅ 3H2 O] is found to be accurate, and evidence from neutron powder diffraction bolsters these assignments. Refined H positions can help understand how H-bonding stabilizes this structure against dehydration to MgCO3 . More broadly, these results illustrate the power of NMR crystallography as a technique for resolving questions where X-ray diffraction is inconclusive.

Solid-State NMR 19F-1H-15N Correlation Experiments for Resonance Assignment and Distance Measurements of Multifluorinated Proteins.

Several solid-state NMR techniques have been introduced recently to measure nanometer distances involving 19F, whose high gyromagnetic ratio makes it a potent nuclear spin for structural investigation. These solid-state NMR techniques either use 19F correlation with 1H or 13C to obtain qualitative interatomic contacts or use the rotational-echo double-resonance (REDOR) pulse sequence to measure quantitative distances. However, no NMR technique is yet available for disambiguating 1H-19F distances in multiply fluorinated proteins and protein-ligand complexes. Here, we introduce a three-dimensional (3D) 19F-15N-1H correlation experiment that resolves the distances of multiple fluorines to their adjacent amide protons. We show that optimal polarization transfer between 1H and 19F spins is achieved using an out-and-back 1H-19F REDOR sequence. We demonstrate this 3D correlation experiment on the model protein GB1 and apply it to the multidrug-resistance transporter, EmrE, complexed to a tetrafluorinated substrate. This technique should be useful for resolving and assigning distance constraints in multiply fluorinated proteins, leading to significant savings of time and precious samples compared to producing several singly fluorinated samples. Moreover, the method enables structural determination of protein-ligand complexes for ligands that contain multiple fluorines.

Jacob Schaefer III

Jacob Schaefer III, 83, died June 27 in Saint Louis, Missouri. “Jake published the first 13 C NMR spectra of synthetic copolymers, the first magic-angle spinning 13 C NMR spectrum of a solid polymer, and the first cross-polarization magic angle spinning (CPMAS) 13 C NMR spectra of polymers. His laboratory produced monumental NMR tools including CPMAS, rotational-echo, double-resonance (REDOR), and multichannel transmission line probes. Today, these tools are so widely used that they are simply referenced by their acronyms, and their impact on medicine, biochemistry, and materials science has been profound. Jake himself applied these techniques to important studies that led to advanced understanding of proteins, living cell metabolism, and polymers.”—Lee G. Sobotka, Lynette Cegelski, Richard Loomis, and Karen Wooley, colleagues Most recent title: Charles Allen Thomas Emeritus Professor of Chemistry, Washington University in St. Louis Education: BS, chemistry, Carnegie Institute of Technology, 1960; PhD, chemistry, University of Minnesota, 1964

Molecular basis of secondary relaxation in stiff-chain glassy polymers.

Recent progress in establishing local order in polycarbonate-like glasses using rotational echo double resonance and centerband-only detection of exchange solid-state nuclear magnetic resonance (NMR) has stimulated a renewed attempt to connect molecular motion within glassy polymers and the mechanical properties of the glass. We have in fact established a correlation between molecular motion characterized by NMR and the mechanical secondary relaxation (tan δ) for nine polycarbonate-like glasses. All of the NMR and mechanical data are for T ≪ Tg. The resulting structural insights suggest that the chains of these polymers are simultaneously both Flory random coils and Vol'kenstein bundles. The cooperative motions of groups of bundles can be described qualitatively by a variety of constrained-kinetics models of the glass. All of the models share a common trait for large-amplitude motion: an exponential increase in the time required for an inter-bundle dilation event with a linear increase in bundle group size. This dependence and a locally ordered Vol'kenstein bundle lead to an understanding of the surprising 60° (K) shift of tan δ to higher temperature for ring-fluoro-polycarbonate relative to that of polycarbonate by the apparently minor substitution of a fluorine for a hydrogen on every fourth ring.

Structure and crystallization behavior of complex mold flux glasses in the system CaO‐Na2O‐Li2O‐CaF2‐B2O3‐SiO2: A multi‐nuclear NMR spectroscopic study

AbstractThe structure of mold flux glasses in the system CaO‐(Na,Li)2O‐SiO2‐CaF2 with unusually high modifier contents, stabilized by the addition of ∼4 mol% B2O3, is studied using 7Li, 23Na, 19F, 11B, and 29Si magic‐angle‐spinning (MAS), and 7Li{19F} and 23Na{19F} rotational echo double‐resonance (REDOR) nuclear magnetic resonance (NMR) spectroscopy. When taken together, the spectroscopic results indicate that the structure of these glasses consists primarily of dimeric [Si2O7]−6 units that are linked to the (Ca,Na,Li)‐O coordination polyhedra, and are interspersed with chains of corner‐shared BO3 units. The F atoms in the structure are exclusively bonded to Ca atoms, forming Ca(O,F)n coordination polyhedra. This structural scenario is shown to be consistent with the crystallization of cuspidine (3CaO·2SiO2·CaF2) from the parent melts on slow supercooling. The progressive addition of Li to a Na‐containing base composition results in a corresponding increase in the undercooling required for the nucleation of cuspidine in the melt, which is attributed to the frustrated local structure caused by the mixing of alkali ions.

Siliceous self-pillared pentasil (SPP) zeolite with incorporated phosphorus groups in catalytic formation of butadiene by dehydra-decyclization of tetrahydrofuran: Study of catalyst stability by NMR and REDOR analysis

Siliceous self-pillared pentasil (SPP) zeolite catalysts with incorporated phosphorus groups were synthesized using four different percentages of P, obtaining a network of nanosheets in a house-of-cards arrangement. The catalysts were used in the dehydra-decyclization of tetrahydrofuran (THF) as biomass-derived molecule to obtain 1,3-butadiene. The presence and stability of phosphate (PO43-) and phosphite (PO33-) groups incorporated in the SPP zeolite framework was evidenced. The analysis of fresh and used catalysts by nuclear magnetic resonance (NMR) tests showed that 95% phosphorus in zeolite catalysts is present as orthophosphate groups and less than 5% as pyrophosphate or other polyphosphate groups. The P bonded to Si (Si-O-P, corresponding to orthophosphate groups), identified by 31 P-{29Si}- Rotational Echo Double Resonance (REDOR) -NMR analysis, are proposed as the active sites responsible for the dehydra-decyclization of THF to obtain 1,3-butadiene. The P-SPP zeolite catalyst with 0.2 ratio of precursors P and Si in the synthesis, presented the best performance.

Network of Bi2O3–Al2O3–NaPO3 glasses studied by solid‐state nuclear magnetic resonance spectroscopy

AbstractA detailed structural investigation of a series of phosphate laser glasses with composition xBi2O3–yAl2O3–(100 − x–y)NaPO3 (x = 0, 10; y = 0, 5, 10, 15, 20) has been conducted using solid‐state nuclear magnetic resonance (NMR) techniques and X‐ray photoelectron spectroscopy (XPS). 31P MAS NMR spectroscopy demonstrates that the addition of Bi2O3 and Al2O3 leads to the depolymerization of the phosphorus chain in the process of Q2 → Q1 → Q0. The various phosphorus species (Qn, n = 0, 1, 2) are identified by 31P single pulse, 31P{27Al} rotational echo adiabatic passage double resonance, 31P{27Al} J‐coupling‐based heteronuclear multiple‐quantum coherence, and 1D refocused INADEQUATE experiments. The results of 23Na{31P}, 31P{23Na}, 27Al{31P} rotational echo double resonance, and XPS consistently confirm that Na+, Al3+, and Bi3+ ions are all bound by phosphorus tetrahedron [PO4]3−. No bond is formed between these glass modifiers. Based on the complementary evidence from previous experiments, comprehensive local structure models are developed for these Bi2O3–Al2O3–NaPO3 glasses.

Direct investigation of the reorientational dynamics of A-site cations in 2D organic-inorganic hybrid perovskite by solid-state NMR

Limited methods are available for investigating the reorientational dynamics of A-site cations in two-dimensional organic–inorganic hybrid perovskites (2D OIHPs), which play a pivotal role in determining their physical properties. Here, we describe an approach to study the dynamics of A-site cations using solid-state NMR and stable isotope labelling. 2H NMR of 2D OIHPs incorporating methyl-d3-ammonium cations (d3-MA) reveals the existence of multiple modes of reorientational motions of MA. Rotational-echo double resonance (REDOR) NMR of 2D OIHPs incorporating 15N- and ¹³C-labeled methylammonium cations (13C,15N-MA) reflects the averaged dipolar coupling between the C and N nuclei undergoing different modes of motions. Our study reveals the interplay between the A-site cation dynamics and the structural rigidity of the organic spacers, so providing a molecular-level insight into the design of 2D OIHPs.

Double echo symmetry-based REDOR and RESPDOR pulse sequences for proton detected measurements of heteronuclear dipolar coupling constants

1H{X} symmetry-based rotational echo double resonance pulse sequences (S-REDOR) and symmetry-based rotational echo saturation pulse double resonance (S-RESPDOR) solid-state NMR experiments have found widespread application for 1H detected measurements of difference NMR spectra, dipolar coupling constants, and internuclear distances under conditions of fast magic angle spinning (MAS). In these experiments the supercycled R412 (SR412) symmetry-based recoupling pulse sequence is typically applied to the 1H spins to reintroduce heteronuclear dipolar couplings. However, the timing of SR412 and other symmetry-based pulse sequences must be precisely synchronized with the rotation of the sample, otherwise, the evolution of 1H CSA and other interactions will not be properly refocused. For this reason, significant distortions are often observed in experimental dipolar dephasing difference curves obtained with S-REDOR or S-RESPDOR pulse sequences. Here we introduce a family of double echo (DE) S-REDOR/S-RESPDOR pulse sequences that function in an analogous manner to the recently introduced t1-noise eliminated (TONE) family of dipolar heteronuclear multiple quantum coherence (D-HMQC) pulse sequences. Through numerical simulations and experiments the DE S-REDOR/S-RESPDOR sequences are shown to provide dephasing difference curves similar to those obtained with S-REDOR/S-RESPDOR. However, the DE sequences are more robust to the deviations of the MAS frequency from the ideal value that occurs during typical solid-state NMR experiments. The DE sequences are shown to provide more reliable 1H detected dipolar dephasing difference curves for nuclei such as 15N (with isotopic labelling), 183W and 35Cl. The double echo sequences are therefore recommended to be used in place of conventional S-REDOR/S-RESPDOR sequences for measurement of weak dipolar coupling constants and long-range distances.

Binding Sites of a Positron Emission Tomography Imaging Agent in Alzheimer's β-Amyloid Fibrils Studied Using 19F Solid-State NMR.

Amyloid imaging by positron emission tomography (PET) is an important method for diagnosing neurodegenerative disorders such as Alzheimer's disease. Many 11C- and 18F-labeled PET tracers show varying binding capacities, specificities, and affinities for their target proteins. The structural basis of these variations is poorly understood. Here we employ 19F and 13C solid-state NMR to investigate the binding sites of a PET ligand, flutemetamol, to the 40-residue Alzheimer's β-amyloid peptide (Aβ40). Analytical high-performance liquid chromatography and 19F NMR spectra show that flutemetamol binds the current Aβ40 fibril polymorph with a stoichiometry of one ligand per four to five peptides. Half of the ligands are tightly bound while the other half are loosely bound. 13C and 15N chemical shifts indicate that this Aβ40 polymorph has an immobilized N-terminus, a non-β-sheet His14, and a non-β-sheet C-terminus. We measured the proximity of the ligand fluorine to peptide residues using 19F-13C and 19F-1H rotational-echo double-resonance (REDOR) experiments. The spectra show that three segments in the peptide, 12VHH14, 18VFF20, and 39VV40, lie the closest to the ligand. REDOR-constrained docking simulations indicate that these three segments form multiple binding sites, and the ligand orientations and positions at these sites are similar across different Aβ polymorphs. Comparison of the flutemetamol-interacting residues in Aβ40 with the small-molecule binding sites in other amyloid proteins suggest that conjugated aromatic compounds preferentially bind β-sheet surface grooves lined by aromatic, polar, and charged residues. These motifs may explain the specificity of different PET tracers to different amyloid proteins.

Off-resonance 13C-2H REDOR NMR for site-resolved studies of molecular motion.

We introduce a 13C-2H Rotational Echo DOuble Resonance (REDOR) technique that uses the difference between on-resonance and off-resonance 2H irradiation to detect dynamic segments in deuterated molecules. By selectively inverting specific regions of the 2H magic-angle spinning (MAS) sideband manifold to recouple some of the deuterons to nearby carbons, we distinguish dynamic and rigid residues in 1D and 2D 13C spectra. We demonstrate this approach on deuterated GB1, H/D exchanged GB1, and perdeuterated bacterial cellulose. Numerical simulations reproduce the measured mixing-time and 2H carrier-frequency dependence of the REDOR dephasing of bacterial cellulose. Combining numerical simulations with experiments thus allow the extraction of motionally averaged quadrupolar couplings from REDOR dephasing values.