In this experimental study, we demonstrate that settling polymethyl methacrylate (PMMA) microparticles with diameters ranging from 6 to 60 µm segregate into distinct bands according to their size when subjected to a rotating laminar annular gap flow with a diverging gap width in the axial direction. Different gap widths ranging from 130 to 1200 µm have been investigated in the fully laminar flow regime. Distinct, spatially separated particle bands of different particle sizes have been observed for nine different geometric configurations, including non-conical, conical, double conical, and variously inclined conical inner cylinder shapes. The study considers different rotation rates, geometric combinations, particle volume fractions, and particle size combinations. Particle size separation was achieved at volume fractions ranging from 2.2% to 11% for rotating inner cylinders. In contrast, no separation occurs during the experimental run when both the outer and inner cylinders are perfectly cylindrical, with no significant variation in the annular gap height. Our experiments also show that rotation of the inner cylinder results in more pronounced particle separation than rotation of the outer cylinder. Microscopic particle image velocimetry (µPIV) measurements show that the presence of particles induces an axial velocity component, which acts as a key transport mechanism. In addition, a significant variation in shear rate is observed across particle bands, which may explain size segregation by shear-induced migration. Furthermore, single particle simulations show that particle trajectories and velocities vary significantly with particle size.