Rosemary Supplementation Research Articles

Effect of Rosemary on Growth Performance, Meat Quality, Fatty Acid Content, Intestinal Flora, and Antioxidant Capacity of Broilers.

Rosemary (Rosmarinus officinalis L.) is a natural spice plant with an aromatic flavor and antioxidant properties that can help enhance the flavor and texture of food, as well as be used as an antioxidant source in pet feed. This study explored the effect of rosemary on the growth performance and antioxidant capacity of broiler chickens. In total, 144 healthy 1-day-old Arbor Acres broilers were randomly divided into four groups: The control group was fed a basic diet, while the positive control group was fed a basic diet supplemented with 30 mg/kg kitasamycin, and the treatment groups were fed a basic diet supplemental with 0.5% rosemary, or 2% rosemary. The average daily feed intake of broilers fed with 0.5% and 2% rosemary in 1-42 days was higher than that in the basal diet group (p < 0.05). The pH was lower in the rosemary groups than in the 30 mg/kg kitasamycin group as measured in the thigh muscle tissue (p < 0.05), and the monounsaturated fatty acid C17:1 heptadecanoic acid content of the 2% rosemary group was higher than that of the other groups (p < 0.05). With 0.5% rosemary supplementation, the activities of the serum and liver antioxidant enzymes catalase (CAT) activity and total antioxidant capacity (T-AOC) increased (p < 0.05); malondialdehyde content decreased (p < 0.05). The serum activities of CAT, total superoxide dismutase, and T-AOC increased with 2% rosemary supplementation (p < 0.05). The relative expression of liver antioxidant genes, the nuclear factor E2-related factor 2, glutathione catalase 1, and superoxide dismutase 1 increased (p < 0.05) with 0.5% rosemary supplementation. The addition of rosemary resulted in higher intestinal lactobacilli counts and lower E. coli counts. In summary, adding 0.5% or 2% rosemary to the diet improved the growth performance of Arbor Acres broilers and increased the number of intestinal probiotics, and supplementing with 0.5% rosemary yielded better results than adding 2% rosemary. This study provides valuable insights into the broader application of plant-derived antioxidants in promoting sustainable and health-focused animal farming practices.

Effect of rosemary (Rosmarinus officinalis) supplement on the growth characteristics and larval metabolism of black soldier fly (Hermetia illucens L.)

Abstract The black soldier fly (BSF), Hermetia illucens. L (Diptera: Stratiomyidae), is often harnessed to transform organic waste into nutrient-rich larval biomass, providing an alternative animal feed in the aquaculture industry. In Israel, rosemary (Rosmarinus officinalis) is a common ornamental plant in gardens that also demonstrates insecticidal and repellent properties and medicinal value. This study focuses on utilizing rosemary waste to produce nutrient-enriched BSF larvae for animal feed and potential medical applications. We evaluated BSF larval survivability, adult emergence and metabolomics following supplementation with different percentages of rosemary leaves (4%, 15%, and 20%, relative to 25 g of the total Gainesville diet). Surprisingly, we observed 95-99.6% larval survivability in the rosemary treatments, comparable to the control. Larval body weight slightly decreased by 4%, 12%, and 20%, respectively, measured as 219 ± 3 mg, 220 ± 3 mg, and 219 ± 4 mg, respectively, compared to the control (249 ± 5 mg) (x̄ ± SE; ). There was a significant difference in adult emergence at 10 days from larval addition (), but no difference after 16 days (). Metabolomics analysis unveiled the over-accumulation of metabolites in BSF larvae after rosemary supplementation linked to aminoacyl-tRNA biosynthesis, glutathione metabolism, phenylalanine metabolism, arginine biosynthesis, phenylalanine, tyrosine, and tryptophan biosynthesis, butanoate metabolism, and arginine and proline metabolism. Conversely, purine metabolism, tyrosine metabolism, and pantothenate and CoA biosynthesis were down-accumulated. Rosemary supplementation also led to the over-accumulation of antioxidant metabolites (rosmarinic acid, carnosic acid, and caffeic acid), suggesting that including rosemary powder in organic compost slightly reduced larval body weight but shortened adult developmental time and enhancing the nutritional value of BSF larvae. However, further molecular studies are necessary to understand BSF resistance and other biochemical changes resulting from rosemary inclusion in their diet.

Effects of dietary water-soluble extract of rosemary supplementation on growth performance and intestinal health of broilers infected with Eimeria tenella.

This study was conducted to explore the effect of dietary supplementation of water-soluble extract of rosemary (WER) on growth performance and intestinal health of broilers infected with Eimeria tenella (E. tenella), and evaluate the anticoccidial activity of WER. 360 1-d-old Chinese indigenous male yellow-feathered broiler chickens were randomly allocated to six groups: blank control (BC) group and infected control (IC) group received a basal diet; positive control (PC) group, received a basal diet supplemented with 200mg/kg diclazuril; WER100, WER200, and WER300 groups received a basal diet containing 100, 200, and 300mg/kg WER, respectively. On day 21, all birds in the infected groups (IC, PC, WER100, WER200, and WER300) were orally gavaged with 1mL phosphate-buffered saline (PBS) of 8 × 104 sporulated oocysts of E. tenella, and birds in the BC group were administrated an aliquot of PBS dilution. The results showed that dietary supplementation of 200mg/kg WER increased the average daily gain of broilers compared to the IC group from days 22 to 29 (P < 0.001). The anticoccidial index values of 100, 200, and 300mg/kg WER were 137.49, 157.41, and 144.22, respectively, which indicated that WER exhibited moderate anticoccidial activity. Compared to the IC group, the groups supplemented with WER (100, 200, and 300mg/kg) significantly lowered fecal oocyst output (P < 0.001) and cecal coccidia oocysts, alleviated intestinal damage and maintained the integrity of intestinal epithelium. Dietary supplementation with WER significantly improved antioxidant capacity, elevated the levels of secretory immunoglobulin A, and diminished inflammation within the cecum, particularly at a dosage of 200mg/kg. The results of this study indicated that dietary supplementation with 200mg/kg WER could improve broiler growth performance and alleviate intestinal damage caused by coccidiosis.

Incorporation of fresh leaves of wormwood (Artemisia herba alba) and/or rosemary (Rosmarinus officinalis) in the diet of rams: Effect on testicular function, sexual behavior, and blood parameters.

This study aimed to investigate the effect of wormwood and rosemary supplementation on some reproductive traits of Barbarine rams. The experiment lasted 2 months. Twenty-four adult rams were divided into four groups (n=6) balanced for the weight (53.3 ± 1.2 kg body weight [BW] ± SD). All rams received 1200 g of straw and 600 g of barley. Control rams (C) without aromatic medicinal plant (AMP), while experimental rams received 20 g of fresh rosemary leaves (R), 20 g of fresh wormwood leaves (A), and 10 g of fresh rosemary leaves plus 10 g of fresh wormwood leaves (RA). The results revealed that the live weight of all rams increased (p < .05) in the RA group compared to the C, A, and R groups. Scrotal circumference increased in the R rams when compared to the controls rams (p < .05). For sperm parameters we showed that the A rams had higher sperm concentrations (p < .05). But, the sperm volume decreased in the R rams (p > .05). However, when the rams received rosemary plus wormwood, their sperm volume increased (p > .05). The sperm mass motility was higher for the A, R and AR rams in comparison to the C rams (p=.05). On the other hand, biochemical analysis of the seminal fluid showed no effect of diets on calcium and total proteins concentration. But the measurement of glucose and seminal insulin showed a decrease (p < .05) in these two biochemical markers in group A rams and a decrease (p < .05) in insulin without modification of the glucose concentration in R rams. Blood glucose and insulin decreased in the animals on AMP diet compared to the other groups (p < .05) while aspartate aminotransferase (AST) increased (p < .05). Rosemary leaves (R and RA groups) increased (p < .05) plasma cortisol compared to the other groups. It can be concluded that the addition of Rosmarinus officinalis and/or Artemisia herba alba in ram diet can have a positive effect on the reproductive function by increasing the concentration and motility of sperm, plasma testosterone, and sexual behavior.

Rosemary (Rosmarinus officinalis L.) essential oil alleviates testis failure induced by Etoposide in male rats

Rosemary (Rosmarinus officinalis L.) is a shrub used to treat hepatic, intestinal, renal, respiratory, and reproductive failures. Etoposide a plant-based compound derived from Podophyllum pelltatum, has been used for human malignancies treatment. However, it induces testis, and hepatic failures. In the present study, impact of rosemary essential oil against testis failure, lipid parameters, and hepatic enzymes in male rats has been studied. Forty male Wistar albino rats were grouped in a completely randomized design with Etoposide injection (ETO), rosemary supplementation (ROS), with Etoposide injection and rosemary supplement (ETO+ROS), and control rats with no Etoposide injection and no rosemary (CON). The experiment lasted for seven consecutive weeks including one week as acclimatization time. At the end of the experiment, rats were sacrificed by cervical dislocation, and blood samples were analyzed for serum alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), low-density lipoprotein-Cholesterol (LDL-C), high-density lipoprotein-cholesterol (HDL-C), total cholesterol (TC), total Protein (TP), glucose (GLU) and testosterone. The left testis was harvested for histological examination. Results showed that rats with Etoposide injection had higher ALT, AST, and ALP the control rats. No significant difference was found among treatments in terms of glucose concentration in blood. Rosemary supplemntaion decreased cholesterol and TG concentration and increased HDL concentration in male rats. Furthermore, administration of rosemary essential oil increased blood testosterone but decreased ALT and AST. The epithelial height of seminiferous tubules was decreased significantly in ET as compared with CON. Rosemary essential oil lessened the adverse effect of Etopside on epithelial height in rat testis as it is shown in ET+ROS. In conclusion, dietary supplementation of rosemary essential oil alleviated liver toxicity and functional testis damage induced by Etopside.

The effect of dietary supplemented dried fennel and rosemary on the performance and caecal microflora of growing rabbits

The objective of this study is to evaluate the possible effect of fennel and rosemary dietary supplements on the performance of rabbits. Therefore fifty-six weaned rabbits (40 days old) from white New Zealand breed were divided into two groups and submitted to the following dietary treatments: A) control diet and B) FR diet consisted of 2.5% Foeniculum vulgare seeds (fennel) and Rosmarinus officinalis leaves (Rosmary) as powder mixed by control diet for thirty days. The growth rate, feed conversion ratio, carcass yield, and mortality were not influenced by dietary fennel and rosemary supplementation. The antimicrobial effect of fennel and rosemary (2.5%) was not observed against E. coli in the caecum of the rabbit treated. The essential oils of Foeniculum vulgare and Rosmarinus officinalis are predominantly composed by Trans Anethole and 1-8 cineole successively. Low antibacterial activity was observed with two essential oils against the strain tested in this study. The addition of the 2.5% combination of fennel and rosemary in rabbit feed did not influence the zootechnical parameters of the rabbits

Rosemary and its protective potencies against COVID-19 and other cytokine storm associated infections: A molecular review

BACKGROUND: Nowadays, medicinal plants have attracted great interest in treatment of human diseases. Rosemary is a well-known medicinal plant which has been widely used for different therapeutic purposes. METHODS: This is a narrative review using databases including PubMed, ISI, Scopus, ScienceDirect, Cochrane, and google scholar, the most authoritative articles were searched, screened, and analyzed. RESULTS: Rosemary is a natural antioxidant which removes reactive oxygen species from tissues and increases expression on Nrf2 gene. Rosemary and its metabolites reduce inflammation by inhibiting production of pro-inflammatory cytokines, decreasing expression of NF-κB, inhibiting infiltration of immune cells to inflamed sites, and affecting gut microbiome. Besides, rosmarinic acid in rosemary extract has positive effects on renin-angiotensin-system. Rosemary affects respiratory system by reducing oxidative stress, inflammation, muscle spasm, and also through anti-fibrotic properties. Carnosic acid is able to penetrate blood-brain-barrier and act against free radicals, ischemia and neurodegeneration in brain. Cardioprotective effects include correcting lipid profile, controlling blood pressure by inhibition of ACE, prevention of atherosclerosis, and reduction of cardiac muscle hypertrophy. CONCLUSIONS: Accordingly, rosemary supplementation has potential protective effects against COVID-19 and other cytokine storm associated infections, a conclusion that needs more evaluations in the next clinical trials.

Thyme (Thymbra spicata L.), rosemary (Rosmarinus officinalis L.) and vitamin E supplementation of laying hens

This study was conducted to determine the effects on performance, egg quality parameters and some serum profiles of vitamin E, thyme and rosemary extracts that were added to laying hen feeds. One hundred twelve 48-week-old Bovans-genotype white laying hens were used in the research. In the study, hens were randomly divided into four groups, each comprising of four replicates of seven hens. The first group was fed with the basal diet as a control. The other three groups were fed diets with 200 mg/kg vitamin E, with 1000 mg/kg thyme, and with 1000 mg/kg rosemary for eight weeks. As a result, vitamin E increased egg production statistically compared with feeds with plant extracts. Thyme-supplemented feed increased egg weight significantly. However, it reduced egg production. Rosemary supplementation generated profit by reducing the feed intake, but had a negative effect by reducing egg weight and egg production. The cholesterol level in the blood serum decreased with thyme supplementation. In addition, blood triglyceride level decreased at a statistically significant level with the supplementation of both thyme and rosemary. In the light of these results, it can be said that the supplementation of vitamin E, thyme and rosemary extracts has a positive effect on some performance parameters and animal health. Since healthier products are obtained from healthy animals, the use of these natural additives in laying hen feeds could be recommended after dose trials.Keywords: aspartate aminotransferase, cholesterol, egg production, feed intake, plant extract, triglyceride

Rosemary supplementation (Rosmarinus oficinallis L.) attenuates cardiac remodeling after myocardial infarction in rats

BackgroundMyocardial infarction (MI) is one of the leading causes of morbidity and mortality worldwide. Dietary intervention on adverse cardiac remodeling after MI has significant clinical relevance. Rosemary leaves are a natural product with antioxidant/anti-inflammatory properties, but its effect on morphology and ventricular function after MI is unknown.Methods and resultsTo determine the effect of the dietary supplementation of rosemary leaves on cardiac remodeling after MI, male Wistar rats were divided into 6 groups after sham procedure or experimental induced MI: 1) Sham group fed standard chow (SR0, n = 23); 2) Sham group fed standard chow supplemented with 0.02% rosemary (R002) (SR002, n = 23); 3) Sham group fed standard chow supplemented with 0.2% rosemary (R02) (SR02, n = 22); 4) group submitted to MI and fed standard chow (IR0, n = 13); 5) group submitted to MI and fed standard chow supplemented with R002 (IR002, n = 8); and 6) group submitted to MI and fed standard chow supplemented with R02 (IR02, n = 9). After 3 months of the treatment, systolic pressure evaluation, echocardiography and euthanasia were performed. Left ventricular samples were evaluated for: fibrosis, cytokine levels, apoptosis, energy metabolism enzymes, and oxidative stress. Rosemary dietary supplementation attenuated cardiac remodeling by improving energy metabolism and decreasing oxidative stress. Rosemary supplementation of 0.02% improved diastolic function and reduced hypertrophy after MI. Regarding rosemary dose, 0.02% and 0.2% for rats are equivalent to 11 mg and 110 mg for humans, respectively.ConclusionOur findings support further investigations of the rosemary use as adjuvant therapy in adverse cardiac remodeling.

Effects of Dietary Canola Seed with Rosemary Supplementation on Growth Performance, Lipid Oxidation and Meat Fatty Acid Composition of Broilers

Effects of Dietary Canola Seed with Rosemary Supplementation on Growth Performance, Lipid Oxidation and Meat Fatty Acid Composition of Broilers

Effect of Different Dietary Levels of Rosemary (Rosmarinus Officinalis) and Yarrow (Achillea Millefolium) on the Growth Performance, Carcass Traits and Ileal Micro-biota of Broilers

The effect of increasing dietary levels of rosemary and yarrow herb powders on the growth performance, carcass traits and ileal microbiota of broilers was studied. Three hundred and thirty-six one-day-old Ross 308 male chickens were allocated to one of the following treatments (six replicates of 8 birds per treatment): control (basal diet with no rosemary or yarrow herbs added), and basal diet with the addition of either 0.5, 1.0 or 1.5% of rosemary herb, or 0.5, 1.0, or 1.5% of yarrow herb. Significant differences were observed (P<0.05) between treatments in the starting (d 1-21) and growing (d 22-42) periods, and in the average feed intake in the starting period. When the whole experimental period was considered, the best-feed conversion rates (P<0.05) were achieved for the highest rosemary and yarrow supplementation levels. Yarrow supplementation resulted in better-feed conversion rates (P<0.05) than the control treatment, for all the periods. No significant differences (P>0.05) were found in the final body weight or in most of the carcass traits. The gastrointestinal tract weight, relative to body weight, increased (P<0.05) due to rosemary and yarrow supplementation, compared with the control treatment. At 42 days of age, the rosemary supplementation increased the Lactobacilli counts and decreased the Escherichia coli counts more than the control and yarrow supplemented treatments. In conclusion, under the conditions of the present work, yarrow supplementation mainly improved growth performance, while rosemary supplementation showed the best effects on ileal microbiota, both compared with the control treatment.

Evaluation of the antioxidant properties and oxidative stability of Pecorino cheese made from the raw milk of ewes fed Rosmarinus officinalis L. leaves

SummaryThe aim of this work was to study the effect of rosemary leaf dietary supplementation on the antioxidant activities and total phenolic content of Pecorino cheese. Three hundred and twenty‐four sheep were randomly assigned to two dietary groups, which received a standard diet based on lucerne hay and concentrate (400 g per day). The concentrate of the rosemary supplemented group contained 2.50% dried rosemary leaves. The trial lasted 7 weeks. Cheesemaking was performed 3, 5 and 7 weeks from the start of the trial. The Pecorino cheese antioxidant activity was modified by the diet. Rosemary supplementation increased the total phenolic content, enhanced the antioxidant properties and decreased the lipid oxidation of cheese. A slight decrease in flavour was detected in cheeses after 7 weeks of rosemary administration.

Oxidative stability of the meat of broilers supplemented with rosemary leaves, rosehip fruits, chokeberry pomace, and entire nettle, and effects on performance and meat quality

Prevention of lipid oxidation needs special attention because a high proportion of fatty acids in broiler meat are unsaturated. A feeding experiment was conducted to evaluate the antioxidant effect of dietary addition of rosemary, chokeberry pomace, rosehip, or nettle in comparison with vitamin E. Male Ross PM3 broilers caged in groups of 6 (4 replicated cages per treatment) were fed a balanced diet supplemented with 25 g/kg of herbal additive, 200 IU of α-tocopheryl acetate/kg, or without supplementation from d 7 to 35. Intake, performance, and with the help of excreta samples, apparent fiber digestibility, ME content, and metabolizability of nitrogen and energy were recorded per cage. Feed was analyzed for total phenols and tocopherols. In each bird (n = 24 per treatment), carcass weight and relative organ weights were recorded, and skin and liver color were assessed. Abdominal fat was analyzed for induction time (h) of lipid oxidation (Rancimat). Breast meat was analyzed for total tocopherol content (mg/kg) and development of TBA reactive substances (TBARS; μg of MDA/kg) over 9 d of storage. Data were subjected to ANOVA considering treatment and, where applicable, storage time. Rosemary supplementation reduced oxidation (TBARS d 9: 201; induction time: 2.48) and elevated tocopherol content (5.72) of the meat compared with control (470, 1.87, and 3.53, respectively). Rosemary-treated birds had a slightly lower carcass weight and a reduced nitrogen and energy metabolizability. Rosehip addition numerically decreased TBARS (319) and enhanced carcass weight (1.71 kg) compared with rosemary-treated birds (1.54 kg). Only a trend in antioxidant activity could be ascribed to chokeberry pomace, although dietary phenolic content was highest. Nettle did not improve oxidative stability (TBARS: 506; induction time: 1.91), although tocopherol content was elevated (6.51). Nettle treatment strongly intensified skin yellowness (b* of 20.6) compared with the control treatment (10.8). Clearly, rosemary is the most suitable dietary antioxidant investigated in this study, yet chokeberry and rosehip also exhibited interesting properties.

Effect of rosemary supplementation on oxidative stress after myocardial infarction in rats

ObjectiveTo analyze the influence of rosemary (R) consumption on cardiac oxidative stress after myocardial infarction (MI).MaterialMale Wistar rats weighing 200g were allocated into 6 groups: 1) Sham group fed standard chow (SA0, n = 6); 2) sham group fed standard chow supplemented with 0.02% of dried R (diet 1) (SA1, n = 6); 3) sham group fed standard chow supplemented with 0.2% of dried R (diet 2) (SA2, n = 6); 4) MI rats fed standard chow (IA0, n = 6); 5) MI rats fed diet 1 (IA1, n = 6); 6) MI rats fed diet 2 (IA2, n = 6). After three months of treatment, heart tissue was collected and tested for lipid hydroperoxide (LH) and antioxidant enzymes catalase, gluthatione peroxidase (GPx), superoxide dismutase (SOD) and Nrf2 expression. Comparisons were made by two‐way ANOVA test and post‐test of Holm‐Sidak. Values were presented as mean ± standard error.ResultsMI rats showed higher cardiac levels of LH and SOD than Sham rats. Rosemary supplementation reduced LH with diet 2 and SOD with both doses. No differences were observed for catalase, GPx and Nrf2.ConclusionChow supplementation with rosemary reduced oxidative stress after myocardial infarction. Financial support: FAPESP.

Effect of feeding goats with rosemary (Rosmarinus officinalis spp.) by-product on milk and cheese properties

The aim of this work was to study the influence on the physicochemical composition and somatic cells count (SCC) of pasteurized goat milk and Murcia al Vino cheese from supplements goat feed with different levels (10 and 20%) of distilled Rosmarinus officinalis spp. leaves. The 20% of rosemary supplementation reduced the milk clotting time, dry matter and lactose milk content. The 10% of rosemary supplementation decreased the percentage of C14 and increased the C18:2 and PUFA content, while the 20% rosemary supplementation decreased the C10 and C14 content, and increased the percentage of C17, C18:2 and PUFA. Significant differences were observed for the protein content, pH and aw of cheeses from the 20% rosemary supplementation. No significant differences were determined for cheese yield, microbiological count and sensory parameters, except for odor between the control and the cheese manufactured with milk from the 10% supplementation group. It is concluded that the milk obtained after rosemary supplementation can be regarded as healthier and shows greater technological suitability for cheese making.

Effect of dietary olive leaves and rosemary on microbial growth and lipid oxidation of turkey breast during refrigerated storage

Forty turkeys were allocated to five groups of eight birds each. One (control) was fed a basal diet while the others were fed diets supplemented with either olive leaves at 10 g/kg, rosemary at 10 g/kg, α-tocopheryl acetate at 150 or at 300 mg/kg feed. Following slaughter, fillets from the breasts of the birds were stored at 4 °C in the dark for 12 days and their lipid oxidation and microbial growth rate were monitored. Results showed that dietary olive leaves were more effective in inhibiting lipid oxidation of the breast fillets compared to rosemary, but inferior to the dietary supplementation of 300 mg α-tocopheryl acetate/kg. In turn, lipid oxidation was more effectively inhibited when the birds received α-tocopheryl acetate supplementation at 150 mg/kg compared to when the birds received no suppelementation, but inferior to rosemary supplementation. Total viable counts, lactic acid bacteria, Enterobacteriaceae and psychrotrophic bacterial counts were all increased in breast fillets of all groups throughout refrigerated storage. Diet supplementation with a basal level of α-tocopheryl acetate had no effect on the bacterial counts recorded in the control group, but diet supplementation with olive leaves or rosemary resulted in a decrease in all bacterial counts from day 2 of storage and thereafter. During this period olive leaves were more effective in inhibiting bacterial growth than rosemaryKeywords: Antioxidant additives, α-tocopherol, turkey meat, herbs, spices, meat quality

로즈마리와 α-Tocopherol Acetate의 급여가 육계의 생산성 및 냉장 저장 중 계육의 품질에 미치는 영향

본 시험은 육계 300수를 로즈마리와 <TEX>$\alpha$</TEX>-tocopherol acetate를 급여하여 5주간 사육한 육계의 생산성과 계육의 TBARS, 총미생물수, pH, 및 육색을 조사하였다. 육질 분석은 계육을 냉장 보관하면서 실험하였다. 시험구는 무첨가구를 대조구(Control)로 하고, 로즈마리 5 g/kg 급여구를 T1, 로즈마리 10 g/kg 급여구를 T2, <TEX>$\alpha$</TEX>-tocopherol acetate 200 mg/kg 급여구를 T3, 그리고 로즈마리 5 g/kg과 <TEX>$\alpha$</TEX>-tocopherol acetate 200 mg/kg 급여구를 T4 등 5개 처리구로 나누어 사양하였다. 육계의 증체량, 사료섭취량 및 사료요구율 등의 생산성은 유의성이 없었다. TBARS는 모든 처리구에서 저장기간이 경과하면서 증가하였고, T2, T3 및 T4에서 대조구와 T1보다 유의하게 낮아 로즈마리와 <TEX>$\alpha$</TEX>-tocopherol은 저장성을 향상시키는 결과이었다. 로즈마리 급여량이 많은 T2가 T1보다, 로즈마리와 <TEX>$\alpha$</TEX>-tocopherol 혼합 급여구에서 TBARS는 낮았다. pH는 저장기간이 지남에 따라 감소하였고, 처리구간의 유의성은 없었다. 총미생물수는 저장기간이 지나면서 모든 처리구에서 증가하였고, <TEX>$\alpha$</TEX>-tocopherol은 미생물의 감소 효과는 없으나 로즈마리와 로즈마리, <TEX>$\alpha$</TEX>-tocopherol 혼합 급여는 대조구와 T3보다 유의하게 낮았다(p<0.05). 육색은 <TEX>$L^*$</TEX>, <TEX>$a^*$</TEX>, 및 <TEX>$b^*$</TEX> 값은 저장하면서 모두 감소하였고, 로즈마리와 로즈마리, <TEX>$\alpha$</TEX>-tocopherol 혼합 급여구에서 <TEX>$a^*$</TEX>값이 유의하게 높았다(p<0.05). 로즈마리 및 로즈마리와 <TEX>$\alpha$</TEX>-tocopherol의 급여구에서 항산화 및 육색의 산화 안정성이 향상되었고, 혼합 급여구에서 시너지 효과가 있었다. The effects of rosemary and <TEX>$\alpha$</TEX>-tocopherol, added individually or in combination, on broiler performance, thiobarbituric acid reactive substance (TBARS), total plate count (TPC) and meat color of chicken thigh meat were investigated. Three hundred broiler chicks divided into five groups were fed a basal diet (control) or basal diet supplemented with 5 g rosemary/kg (T1), 10 g rosemary/kg (T2), 200 mg <TEX>$\alpha$</TEX>-tocopherol/kg (T3), or 5 g rosemary/kg + 200 mg <TEX>$\alpha$</TEX>-tocopherol/kg (T4) for 5 weeks. Following slaughter, chicken meat was stored at <TEX>$4^{\circ}C$</TEX> for 10 days. All treatments did not influence the performance. Rosemary supplementation delayed lipid oxidation in thigh meat during refrigerated storage. T2 was significantly (p<0.05) more effective in delayed lipid oxidation compared to T1, but was inferior to T3. Samples containing a combination of antioxidant had lower TBARS values than those containing the individual antioxidants, indicating a synergistic effect. TPC was significantly increased (p<0.05) in thigh meat of all groups throughout the refrigerated storage. The T3 and control groups showed TPC counts that did not differ from each other during the entire storage period. However, rosemary supplementation was associated with bacterial counts that were significantly lower (p<0.05) than the control and <TEX>$\alpha$</TEX>-tocopherol groups at day 3 of storage and thereafter. For this period, T1 presented TPC counts that were significantly higher than the T2 group (p<0.05). At all storage times, the thigh meat of rosemary-fed chickens was redder than control (higher <TEX>$a^*$</TEX>), while no differences in <TEX>$L^*$</TEX> and <TEX>$b^*$</TEX> values were found. A synergistic effect was obtained from the combination of rosemary with <TEX>$\alpha$</TEX>-tocopherol, whereas individual use of the antioxidants significantly improved color stability compared to the control.

Anti-oxidant supplementation improves boar sperm characteristics and fertility after cryopreservation: Comparison between cysteine and rosemary ( Rosmarinus officinalis)

Anti-oxidants partially ameliorated the detrimental effects of reactive oxidative substances produced during cryopreservation. The objective of the study was to determine the effect of anti-oxidant addition to the freezing extender on boar semen qualities and fertility capacity. Ejaculates were collected from a previously selected boar and semen samples were processed using the straw freezing procedure. In experiment 1, semen samples were cryopreserved in lactose–egg yolk solution supplemented with various concentrations of cysteine (0, 5 and 10 mM) to determinate a cysteine concentration capable of producing a protective effect during cryopreservation. Semen quality (total motility, progressive motility, viability, acrosome integrity and hypoosmotic swelling test) was evaluated after freezing and thawing and then every hour for 3 h. In experiment 2, ejaculates were cryopreserved with lactose–egg yolk extender with or without the following anti-oxidants: cysteine, rosemary ( Rosmarinus officinalis) and cysteine plus rosemary. Semen quality was evaluated. In the experiment 3, fertility capacity of semen frozen in anti-oxidant supplementation extenders was examined in vitro. A total of 2232 oocytes were in vitro matured and inseminated with frozen–thawed sperm. In summary: (i) the effective concentration of cysteine in freezing extender was 10 mM; (ii) the addition of exogenous rosemary or cysteine to the freezing extender positively affected post-thawed viability and acrosome integrity. Only rosemary supplementation improved total motility at 3 h and progressive motility at any time; (iii) the inclusion of rosemary into the extender was effective in penetration and cleavage rate and also in the efficiency of the fertilization system.

Rosemary extract (Rosmarinus officinalis L.) supplementation into the diet of Nero Siciliano pigs: effects on lipid oxidation

During the growing-fattening period (93 days; ILW 33.5±6 kg to FLW 67±3 kg), 30 Nero Siciliano pigs were fed on a basal diet supplemented with (ROX group) or not (CTR group) a rosemary extract (1g?kg-1). At 1, 3 and 5 days after slaughtering, the oxidative stability of the Longissimus dorsi muscle was determined by using TBArs test. Moreover, on the individual samples of the muscle the lipid content, the fatty acid and the sterol composition were determined; the acidic composition of the lard, removed from backfat, was also studied. Data were subjected to ANOVA. The fat content of the muscle was unaffected by the dietary treatment as well as the oxidative stability whereas, significant differences (P≤0.01) were observed for the polyunsaturated fatty acid content which showed the highest values in the meat as well as in the lard of the ROX group; PUFA/SFA ratio was also significantly highest in the muscle (P=0.004) and in the lard (P=0.017) of the ROX group, testifying a possible antioxidative activity of the rosemary extract. The sterol fraction (cholesterol, cholestanol, stigmasterol, beta-sitosterol and delta 5-avenasterol) of the Longissimus dorsi muscle was unaffected by the rosemary supplementation; a significant difference was observed only for the campesterol (CTR group=1.08, ROX group=0.90; P = 0.021).