Rosehip Research Articles

Effect of storage time and temperature on stability of bioactive compounds in aseptically packed beverages prepared from rose hips and sea buckthorn berries

Rose hips and sea buckthorn berries contain high amounts of beneficial bioactive compounds. In this study the content and stability of natural vitamins and antioxidants were investigated in two fruit beverages made from rose hip powder, sea buckthorn berry purée and white grape juice concentrate, and wheat germ oil in one of the beverages. Beverages were formulated, pasteurised and filled aseptically in Tetra Brik packages and stored up to 35 days at 4 °C or 22 °C. Samples were analysed for the content of ascorbic acid, carotenoids, major phenolics, tocochromanols, total phenols and total antioxidant capacity as ferric reducing ability. The largest changes occurred during the first 5 days of storage, thereafter mainly ascorbic acid decreased. In the wheat germ oil supplemented beverage, α-tocopherol was more rapidly degraded. Negative correlations between the decrease of some carotenoids and tocochromanols, and the increase of some phenolics, suggest relationships of redox reactions specific to the amount of oil supplemented. From the present study we can conclude that additions of oil to beverages should be carefully selected to avoid oxidations of bioactive compounds. Furthermore, packaging of the beverages should be carried out at higher temperatures and thereafter it should be stored at low temperatures (4 °C), which decreased oxidation of all bioactive compounds except ascorbic acid.

Extraction and Characterization of Phenolic Compounds from Rose Hip (<i>Rosa canina</i> L.) Using Liquid Chromatography Coupled with Electrospray Ionization - Mass Spectrometry

Wild berry are a rich of natural compounds which provide them high antioxidant potential. The compounds which provide them these proprieties are known to be vitamins, flavonoids, anthocyanins and phenolic acids. The aim of this study was to extract and characterize bioactive compounds from rose hip (Rosa canina L.) currently found in Romania. A qualitative high-performance liquid chromatography coupled with electrospray ionization mass spectrometric (ESI-MS) detection in positive ion mode has been used to identify phenolic compounds from rose hip crude extract. The chromatograms revealed the presence of a large number of compounds (19), identified and grouped as phenolic acids and flavones/ols, flavan-3-ols and also anthocyanins. Based on obtained results these berries can be highly recommended as part of our diet. Also this finding represents a contribution to the chemical characterization of phenolic profile of rose hip.

Extraction and Characterization of Phenolic Compounds from Rose Hip (<i>Rosa canina</i> L.) Using Liquid Chromatography Coupled with Electrospray Ionization - Mass Spectrometry

Extraction and Characterization of Phenolic Compounds from Rose Hip (<i>Rosa canina</i> L.) Using Liquid Chromatography Coupled with Electrospray Ionization - Mass Spectrometry

Effects Of Rose-Hip And Grapeseed Dietary Supplementation On Serum Oxidative Stress Parameters In Dogs Before And After Physical Exercise

Abstract The aim of this study was to evaluate the effects of 60 days of rose-hip and grapeseed dietary supplementation of a balanced home-cooked diet on serum oxidative stress parameters: ROMs, MDA and FRAP in army service dogs before and after regular physical exercise. The dogs were fed a balanced cooked diet as instructed by army standards until the initial blood sampling in June. Thereon the dogs were randomly allotted to 4 groups according to the dietary regime: dogs maintained on a balanced cooked diet according to army standards, branded dry dog food, cooked diet with added 500 mg rose-hip extract, and cooked diet with added 100 mg grapeseed extract for a 60 day period from June to September after which all 4 groups were fed the standard cooked meal diet. Sampling was performed at the beginning of the experiment (June), 60 days from the start of the treatment (September) and finally 60 days after the end of supplementation (November). Statistical analysis of the results included descriptive statistical parameters: mean (M), standard deviation (SD), and variation coefficient (CV%). In order to test the statistical significance of the differences between treatments a multifactor variance test (ANOVA) was performed for ROM, MDA and FRAP and the combined effects of diet, exercise and time period were observed. The initial (in June) increase in MDA and ROMs after exercise indicates the presence of oxidative stress 30 minutes after exercise. However, the antioxidative effects of rose-hip and grapeseed extracts are not conclusive, as multifactor ANOVA testing of time, diet, and exercise factors did not reveal for MDA statistically significant differences either at 60 days of supplementation nor 60 days after withdrawal of the supplements. Only one distinct exception was recorded for the prolonged antioxidative effects reflected in significantly decreased (p<0.01) ROMs before and after exercise in dogs fed the branded dry food 60 days after the end of such dietary regime (November). FRAP values tend to be higher (p>0.05) after exercise compared to before exercise in all experimental groups in November, indicating on a possible redistribution and upregulation of endogenous antioxidants during the experiment.

Rose hip powder made from a subspecies of Rosa canina (containing seeds and shells) reduces symptoms of osteoarthritis and the need for pain killers

Rose hip powder made from a subspecies of Rosa canina (containing seeds and shells) reduces symptoms of osteoarthritis and the need for pain killers

Extraction of Minor Compounds (Chlorophylls and Carotenoids) from Yarrow–Rose Hip Mixtures by Traditional versus Green Technique

AbstractNowadays, various branches from food industry generate large amount of by‐products that are often underutilized, which could have negative environmental impact and increase the price of the raw material as it is not completely utilized in the production. Mixtures of two interesting plants (yarrow and rose hip) with different ratios, obtained as by‐products from filter‐tea factory, have been extracted with organic solvents and with environmentally friendly technology, supercritical fluid extraction (SFE). In the obtained extracts, quantitative content of minor plant pigments (chlorophylls and carotenoids) was determined and technologies were compared. Moreover, the effect of SFE parameters on yields of chlorophylls and carotenoids was determined. The results showed that SFE could be used as a good alternative for extraction of plant pigments from Achillea millefolium L. and Rosa canina L. mixtures. Besides plant pigments, the obtained extracts were rich in other nonpolar compounds from plant mixtures. Utilization of herbal waste provided value‐added products, rich in various health benefit compounds.Practical ApplicationsCurrently, there is growing interest for developing economically feasible and environmentally friendly processes for extraction of bioactive compounds from plant material. Moreover, sustainable and relatively cheap raw material should be utilized in the production. Therefore, waste and by‐products from the food industry, e.g., plant material from filter‐tea factory with small mean particle size, could be used. Traditional technologies used for extraction of chlorophylls and carotenoids are no longer environmentally acceptable and alternative green technologies, such as supercritical fluid extraction, should be considered. This research provides a comparison of green and traditional processes for plant pigment extraction and studies effects of process parameters (pressure and temperature) on yields of the investigated compounds. It also utilizes by‐products of two medicinal plants (yarrow and rose hip) as raw material for extraction as herbal dust generated in filter‐tea factory could not be used for tea bags filling.

Bioactive and rheological properties of rose hip marmalade.

In this study, bioactive (total phenolic, antioxidant and antiradical activity) and rheological properties (steady and dynamic) of rose hip marmalade were investigated. Bioactive properties were determined in rose hip marmalade and extract. Extract had higher total phenolic content (38.5mg GAE/g dry extract), antioxidant activity (124mg AAE/g dry extract) and antiradical activity (49.98%) than marmalade. Steady and dynamic rheological properties of the marmalade were determined at different temperature levels (5, 25 and 45°C). Rose hip marmalade exhibited shear thinning behavior and Ostwald de Waele model best described flow behavior of the sample (R (2) ≥0.9880) at different temperature levels. Consistency index and apparent viscosity values (η 50 ) at shear rate 50s(-1) decreased with increase in temperature level. Viscoelastic properties were determined by oscillatory shear measurements and G' (storage modulus) values were found to be higher than G'' (loss modulus) values, indicating that the rose hip marmalade had a weak gel-like structure with solid-like behavior. G', G'', G (*) (complex modulus) and η* (complex viscosity) values decreased with increase in temperature level. Modified Cox-Merz rule was satisfactorily applied to correlate apparent and complex viscosity values of the rose hip marmalade at all temperatures studied.

Daily intake of rosehip extract decreases abdominal visceral fat in preobese subjects: a randomized, double-blind, placebo-controlled clinical trial.

BackgroundObesity has become a great problem all over the world. We repeatedly screened to find an effective food to treat obesity and discovered that rosehip extract shows potent anti-obesity effects. Investigations in mice have demonstrated that rosehip extract inhibits body weight gain and decreases visceral fat. Thus, the present study examined the effect of rosehip extract on human body fat in preobese subjects.MethodsWe conducted a 12-week, single-center, double-blind, randomized, placebo-controlled study of 32 subjects who had a body mass index of ≥25 but <30. The subjects were assigned to two random groups, and they received one tablet of placebo or rosehip that contained 100 mg of rosehip extract once each day for 12 weeks with no dietary intervention. Abdominal fat area and body fat percent were measured as primary outcomes. The other outcomes were body weight and body mass index.ResultsAbdominal total fat area, abdominal visceral fat area, body weight, and body mass index decreased significantly in the rosehip group at week 12 compared with their baseline levels (P<0.01) after receiving the rosehip tablet intake, and the decreases in these parameters were significantly higher when compared with those in the placebo group. Additionally, body fat percent tended to decrease compared with the placebo group and their baseline level. Moreover, the abdominal subcutaneous fat area was significantly lower in the rosehip group than in the placebo group at week 12 after the initiation of intake (P<0.05). In addition, there were no abnormalities, subjective symptoms, and findings that may indicate clinical problems during the study period.ConclusionThese results suggest that rosehip extract may be a good candidate food material for preventing obesity.

Frost decreases content of sugars, ascorbic acid and some quercetin glycosides but stimulates selected carotenes in Rosa canina hips

Primary and secondary metabolites of Rosa canina hips were determined by HPLC/MS during ripening and after frost damage. Rose hips were harvested six times from the beginning of September until the beginning of December. Color parameters a*, b* and L* decreased during maturation. Glucose and fructose were the predominant sugars representing up to 92% total sugars, and citric acid was the major organic acid detected in rose hips (constituting up to 58% total organic acids). Total sugar and ascorbic acid content significantly decreased after frost damage; from 42.2 to 25.9 g 100 g(-1) DW for sugars and from 716.8 to 176.0 mg 100 g(-1) DW for ascorbic acid. Conversely, β-carotene and lycopene levels increased in frostbitten rose hips to 22.1 and 113.2 mg 100 g(-1) DW, respectively. In addition to cyanidin-3-glucoside (highest level in hips was 125.7 μg 100 g (-1) DW), 45 different phenolic compounds have been identified. The most abundant were proanthocyanidins (their levels amounted up to 90% of total flavanol content) and their content showed no significant differences during maturation. The levels of catechin, phloridzin, flavanones and several quercetin glycosides were highest on the first three sampling dates and decreased after frost. Antioxidant capacity similarly decreased in frostbitten rose hips. Total phenolic content increased until the third sampling and decreased on later samplings.

Rose hip (Rosa canina L): A functional food perspective

Background: Rose hip (Rosa canina L.) is the pseudo-fruit of the rose plant, which is widely known as a valuable source of polyphenols and vitamin C. Both in vivo and in vitro studies have demonstrated that this fruit exhibits anti-inflammatory, antioxidant, and antiobesogenic activities. The health benefits of Rose hip (RH) have been attributed to its wide range of bioactive compounds including the anti-inflammatory galactolipid: (2S)-1,2-di-O-[(9Z,12Z,15Z)-octadeca-9,12,15-trienoyl]-3-O- β-D-galactopyranosyl glycerol (GOPO), vitamin C, phenolics, lycopene, lutein, zeaxanthin, and other carotenoids. As cyclooxygenase inhibitors, RH compounds may reduce the risk of cancer, heart disease, and various inflammatory conditions. The aim of this review is to present an overview of the functional, medical, and physiological properties of RH.Keywords: Rosa canina, Rose hip, antioxidant, anti-inflammatory, GOPO, lycopene, and vitamin C

Late laying hens deposit dietary antioxidants preferentially in the egg and not in the body

The allocation of nutrients in the metabolism of laying hens favors the egg over the body. It is unclear whether this programming also includes micronutrients and antioxidants. This was tested with five by seven 76-wk-old Heisdorf & Nelson Brown Nick layers. They were fed a basal diet low in antioxidants either unchanged (control) or supplemented with 40 IU of α-tocopherylacetate/kg of diet (vitamin E) or 2.5% chokeberry pomace, rose hip, or sage in the last 4 wk before slaughter. The traits measured were subjected to ANOVA. The additives did not affect ADFI, performance, egg quality, carcass, or meat quality. Yolk tocopherol contents were higher with vitamin E and sage compared with control and rose hip treatments. Oxidative stability was more affected in egg yolk powder than in the meat. After 12 wk of storage, yolks from vitamin E-treated hens were lowest in TBA levels (5.0 mg of malondialdehyde/kg), followed by chokeberry (8.7) and sage (8.8). Rose hip (18.1) and control (18.9) treatments were similar. In meat, TBA was slightly decreased after 9 d of storage in meat from chokeberry- and sage-supplemented hens (contrast analysis). In conclusion, antioxidant deposition to the egg seems to be part of the genetic determination of the hen. Adding antioxidants is therefore interesting for layer nutrition in general, and herbal additives allow performing this in a natural way. The programming for partitioning, however, renders the strategic feeding of antioxidants before slaughter, with the goal to increase oxidative stability of spent hen meat rather inefficient.

A Pilot Study on the Effectiveness of a Rose Hip Shell Powder in Patients Suffering from Chronic Musculoskeletal Pain

We carried out a 3-month preliminary investigation on the effectiveness of a rose hip shell powder and its mechanism of action. Of 52 patients suffering from acute exacerbations of low back pain (n = 39) or knee pain (n = 13), 29 had participated earlier in the pilot study with the pseudofruit powder Litozin(®) . After assessing the baseline values, patients were offered up to 20 g of a rose hip shell powder per day. Patients were encouraged to adjust the daily dose upwards or downwards according to their symptoms for the period of 3 months. The examination for possible effectiveness was by intention-to-treat analysis with last observation carried forward. There was no difference in any generic or disease-specific outcome variables between the patients consuming the rose hip shell powder and those consuming the pseudofruit powder Litozin(®) in the previous surveillance study. A human protein array system and fractions from the rose powders were used to study their effect on cytokine expression in vitro. The data indicate that lipophilic rose hip fractions from the shell and the pseudofruit inhibit cytokine expression and that the shell powder may be the better starting material for a future rose hip extract prepared with a lipophilic solvent.

Nutrient composition of selected traditional United States Northern Plains Native American plant foods

Ten wild plants (cattail broad leaf shoots, chokecherries, beaked hazelnuts, lambsquarters, plains prickly pear, prairie turnips, stinging nettles, wild plums, raspberries, and rose hips) from three Native American reservations in North Dakota were analyzed to expand composition information of traditional foraged plants. Proximates, dietary fiber (DF), vitamins, minerals, carotenoids, and folate vitamers were assayed using standard methods and reference materials. Per serving, all were rich in Mn (100–2808μg). Several provided >10% DRI of Fe (cattail shoots, steamed lambsquarters, and prairie turnips), Ca (steamed lambsquarters, prickly pear, and prairie turnips), Mg (cattail shoots, lambsquarters, prickly pear, and prairie turnips), vitamins B6 (chokecherries, steamed lambsquarters, broiled prickly pear, and prairie turnips), C (raw prickly pear, plums, raspberries, rose hips (426mg/100g), and K (cattail shoots, chokecherries, lambsquarters, plums, rose hips, and stinging nettles). DF was >10g/serving in chokecherries, prairie turnips, plums and raspberries. Rose hips, plums, lambsquarters, and stinging nettles were carotenoid-rich (total, 3.2–11.7mg/100g; β-carotene, 1.2–2.4mg/100g; lutein/zeaxanthin, 0.9–6.2mg/100g) and lycopene (rose hips only, 6.8mg/100g). Folate (primarily 5-methyltetrahydrofolate) was highest in raw lambsquarters (97.5μg/100g) and notable in cattail shoots, raw prairie turnips, and blanched stinging nettles (10.8, 11.5, and 24.0μg/100g, respectively). Results, provided to collaborating tribes and available in the National Nutrient Database of the United States Department of Agriculture (USDA) (www.ars.usda.gov/nutrientdata), support reintroduction or increased consumption of foraged plants.

Investigation of the morphology, biology and infestation of Mecorhis ungarica (Herbst, 1783) (Coleoptera: Rhynchitidae) associated with rosehip plants (Rosa spp.)

Kusburnu, Turkiye’de sosyo-ekonomik acidan onemli bir bitkidir. Bu calisma Erzincan ilinde 2007-2008 yillarinda, kusburnu bitkisi uzerinde zarara sebep olan Mecorhis ungarica (Herbst, 1783) (Coleoptera: Rhynchitidae) ’nin morfolojisi, biyolojisi ve zararinin belirlenmesi amaci ile yurutulmustur. Arastirmalar; arazi surveyleri, biyolojik calismalar ve morfolojik calismalar olmak uzere uc kisimda gerceklestirilmistir.Arastirmanin sonuclarina gore, M. ungarica disilerinin yumurtalarini kusburnu bitkisinin tomurcuk veya meyvesi icerisine biraktigi, yumurtalarin 8-12 (10,1) gunde acildigi, dogada yumurta birakmanin 40-45 (40) gun surdugu, bir disinin gunluk yumurta veriminin 1,21,6 (1,4) oldugu, larvanin meyve icerisinde beslenerek olgun hale geldigi, agustos ayinin ikinci haftasindan itibaren topraga gecerek bir kese hazirlayip kisi bunun icerisinde gecirdigi tespit edilmistir. Erken ilkbaharda nisan ayinin ucuncu haftasinda pupa donemine gectigi, pupa suresinin 8-12 (9,9) gun oldugu ve mayis ayinin son haftasindan itibaren erginlerin topraktan cikis yaptigi belirlenmistir. M. ungarica Erzincan ekolojik kosullarinda yilda 1 dol vermektedir. Erzincan Ilinde 2007 yili survey calismasi sonuclarina gore; kusburnu tomurcuklarinin %18’i ve meyvelerin %31,7’si M. ungarica’nin yumurta, larva veya erginlerinin zararina maruz kaldigi, genel bulasiklik oraninin ise %24,85 oldugu tespit edilmistir

Rose hip alleviates pain and disease progression in rats with monoiodoacetate induced osteoarthritis

Although rose hip administration reduces pain and stiffness in patients with osteoarthritis, the mechanism of rose hip to alleviate primary symptoms has not been investigated. We examined how two types of Rosa canina, grown in Denmark (I-Flex) and Coesam in Chile, attenuate the osteoarthritis symptoms in male rats with osteoarthritis. Therefore pain-related behaviors were evaluated, and histological changes and cytokine expression in the articular cartilage of right knee induced osteoarthritis were assessed via intra-articular monoiodoacetate (MIA) injection. The right knee induced swollen knee, limping legs, and disproportional weight distribution into the right hind paw, and reduced maximum velocity to run on a treadmill from day 3 after MIA injection. The symptoms were exacerbated up to about 2 weeks and remained steady until day 21. Overall the osteoarthritis symptoms in MIA-injected control rats did not significantly change over the experimental period. After 21 days, bone mineral density in right leg and knee had greatly decreased in rats injected with MIA. These symptoms were related to increased expression of matrix metalloprotinase (MMP)-3 and MMP-13 in articular cartilage that degraded collage and elevated the production of TNF-α, IL-1β, and IL-6. However, both types of rose hip markedly attenuated all of the symptoms experienced by the control and overall symptoms from day 3 were improved at day 21. However, some symptoms of osteoarthrit remained and there was no difference between both rose hips for alleviating osteoarthritis symptoms. In conclusion, rose hips from Denmark and Coesam in Chile are potential therapeutic agents for the protection of articular cartilage against progression of osteoarthritis.

Biological activities of selected polyphenol-rich fruits related to immunity and gastrointestinal health

Small fruits are a rich source of bioactive substances, including polyphenols, and are therefore suitable raw materials for the production of functional foods. In the current work, we studied the antioxidative properties of six fruits: rosehip, chokeberry, hawthorn, blackcurrant, blueberry and rowanberry via different methods (ORAC, TRAP, HORAC and inhibition of lipid peroxidation). Their effect on the production of reactive oxygen species (ROS) by phagocytes, antimicrobial properties against 11 human pathogens, and mitogenic effect on hamster spleen lymphocytes were also tested. Rosehip extract showed the highest antioxidant activity via ORAC, TRAP and HORAC assays, whereas blueberry extract was the most potent inhibitor of lipid peroxidation. All extracts inhibited ROS production of opsonized zymosan-activated phagocytes, indicating that extracts interfere with the signaling cascade of phagocyte activation upstream to the protein kinase C activation. Chokeberry, blackcurrant and rowanberry extracts revealed strong antimicrobial properties against a broad spectrum of microorganisms and also had the highest mitogenic activity.

PACR (Pine Bark Extract, L Arginine, L Citrulline, Rose Hip Extract)Improves Emotional, Physical Health and Sexual Function in Peri-Menopausal Women

Objective: Objective of the study was to evaluate the effect of PACR on sexual function and climacteric symptoms in peri-menopausal women. In addition, data of clinical chemistry and antioxidative capacity were collected. Study design: In the double-blind, placebo-controlled study, 80 peri-menopausal women were supplemented with 4 tablets Lady Prelox® (PACR: Pine Bark Extract, L-Arginine, L-Citrulline, Rose Hip Extract) the day. Sexual function was evaluated by the Female Sexual Function Index (FSFI). Climacteric symptoms were ascertained by Women’s Health Questionnaire and questions from the Kupperman’s Index. Antioxidative activity and clinical chemistry was measured in blood samples of the participants by FSFI. Results and conclusion: PACR improved sexual function as measured by 60% after 1 month and by 73% after 2 months treatment with PACR compared to baseline, increase of FSFI in the placebo group was only 40 and 46%. PACR relieved also climacteric symptoms evaluated by the Kupperman’s index and Women’s Health Questionnaire (WHQ). The efficacy of PACR was significantly superior compared to placebo. PACR also influenced metabolic parameters and antioxidant capacity of blood positively. No unwanted effects were reported. PACR improves sexual function and relieves climacteric symptoms in peri-menopausal women.

A novel rose hip preparation with enhanced anti-inflammatory and chondroprotective effects.

Rose hip powder (RHP) alleviates osteoarthritis (OA) due to its anti-inflammatory and cartilage-protective properties. Substances contained in RHP might contribute to its clinical efficacy. The activity of two RHP (i.e., RH-A, from the whole fruit, RH-B, from fruits without seeds) was investigated in human peripheral blood leukocytes (PBL) and primary chondrocytes (NHAC-kn). RH-A and RH-B diminished the secretion of chemokines and cytokines in LPS/IFN-γ-activated PBL, including CCL5/RANTES, CXCL10/IP-10, interleukin- (IL-) 6, and IL-12. Most effects were transcriptional, since gene expression levels were significantly influenced by RH-A and RH-B. In IL-1β treated normal chondrocytes (NHAC-kn), both RH preparations reduced the expression of matrix metalloproteinase- (MMP-) 1, MMP-3, and MMP-13 and ADAMTS-4. These changes are associated with diminished inflammatory damage or cartilage erosion. Principal component analysis revealed that (1) RH-A and RH-B modified a large pattern of biomarkers, and (2) RH-B outperformed RH-A. Furthermore, RH-B contained more chondroprotective and anti-inflammatory constituents than RH-A. Thus, RHP contributed to restore cellular homeostasis in PBL and chondrocytes. RH preparations from fruits without seeds are thus expected to have an improved OA-preventive or OA-therapeutic profile, as subsequently shown in a related clinical trial.

Field Strategies for Rose Hip Production in Prince Edward Island

Market demand for rose hips from wild rose (Rosa spp.) plants is increasing as research reveals the valuable nutraceutical compounds that they contain. Roses grow wild throughout Prince Edward Island, Canada, and commercial production of rose hips is a recently new venture in this province. This study examined the long-term effects of several management practices on rose hip production in Prince Edward Island. Cuttings from native wild populations were planted in a replicated trial at the Agriculture and Agri-Food Canada, Crops and Livestock Research Centre, Harrington Research Farm in Harrington, Prince Edward Island, in 2004. Treatments consisted of in-row mulch (none, straw, or bark); fertility (none, compost, or fertilizer); and two inter-row (tilled or sod). Mulch, especially straw mulch, promoted overall plant size and yield, while fertilizer promoted plant height and yield. Inter-row tilling was best during the first years of growth, while inter-row sod led to increased plant height in later years. Results indicated that management practices may need to be adjusted as plant establishment proceeds in order to maintain healthy and productive plants for long-term commercial production.

퍼머넌트 웨이브와 산성 염색 반복시술시 로즈힙 추출물 첨가의 효능

Abstract In this research, with a goal of lessening the damage to hair by adding rose hip extract to permanent wave 1 solution and performing permanent wave proc edure prior to acid dyeing and maximizing the effect of the procedure, rose hip ext ract 15%, 20% was added to each thioglycolate 1 solution and cysteine 1 solution and the procedure was performed. As a result, when performing the procedure by adding rose hip extract to permanent wave 1 solution thioglycolate and cysteine permanent wave 1 solution , the effect of wave effectiveness is increased and the damage to hair was confirmed to be low. Depending on the rose hip additive status in thioglycolate, the treatment effect of hair was shown to be beneficial. However, the limit of this research is that the thickness of hair strands for each person differs as well as the location of the hair contributing to the difference and therefore exact judgement regarding the shape of waves as well as the level of damage cannot be fully measured. In the following clinical tests, we w ill test the procedure on variety of hair types. Key Words : Permanent wave, Acid dye, Rosehip Extract.