Rosehip Research Articles

In Vitro Functional Properties of Rosehips from 'Aurora' Edible Garden Rose's Collection.

Although they have been extensively studied in many species of the genus Rosa L., garden roses' hips have largely been overlooked. To investigate their potential use in the food industry, this study evaluated five cultivars from 'Aurora' collection: 'Purple Aurora', 'Berry Bush Aurora', 'Aromatic Aurora', 'Butterfly Aurora', and 'Rugose White Aurora'. Morphological characterization, along with the assessment of the phenolic profile, vitamin C levels, and biological activities-including antioxidant and neuroprotective effects-was conducted. The fruit mass reached 5.15 g, while the mesocarp mass ranged from 3 to 4 g, resulting in a mesocarp-to-fruit ratio of over 75%. The total phenolic content ranged from 37.1 to 63.9 mg GAE/g de, while total flavonoids were present in amounts from 0.85 to 2.14 mg QE/g de. Rosehip extracts from four out of five cultivars exhibited a very high vitamin C content, reaching 2384 µg/g fw. Quinic acid and 31 phenolic compounds were found in the rosehip extract of at least one cultivar. Results indicated 'Aurora' rosehips have potent antioxidant properties and a moderate inhibitory effect on acetylcholinesterase, highlighting their potential as a source of functional food. Additional research is needed to fully leverage these benefits and establish garden rosehips as a viable alternative to synthetic antioxidants.

Biosynthesis of cobalt oxide nanoparticles and their cytotoxicity against cancer cells mouse colon adenocarcinoma cell line

The application of plant extracts for the biosynthesis of cobalt oxide nanoparticles is eco-friendly, more economical, less toxic, and more biocompatible. In this study, we used Rose hip extract to synthesize cobalt oxide nanoparticles (Co3O4 NPs). The biosynthesized cobalt oxide nanoparticles were characterized by FT-IR spectroscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM). The PXRD and FTIR analyses confirmed the successful synthesis and the characteristic bands of Co3O4 NPs, respectively. The FESEM image showed that the Co3O4 NPs were approximately spherical morphology with a mean size of 90.77 ± 32.35 nm. The EDX analysis showed the elemental composition contains carbon in the formulation of Co3O4 NPs. The results showed that the plant extracts can be used to synthesiz cobalt oxide nanoparticles with good quality at nanoscale sizes. The as-prepared Co3O4 NPs exhibited excellent cell toxicity properties against mouse colon adenocarcinoma cells, indicating that biosynthesized cobalt oxide nanoparticles have potential applications in cancer and nanomedicine.

Formulation, Standardization, and Characterization of a Novel Herbal Medicine Tablet Containing Rosehip Extract

The fruit of Rosa canina L., commonly known as rosehip, has a long history of use in treating various disorders such as osteoarthritis and cardiovascular diseases. However, like many other herbal extracts, rosehip dried extract presents challenges due to its hygroscopic and sticky properties. This poses difficulties in developing solid pharmaceutical dosage forms utilizing rosehip extract. Hence, the objective of this study was to formulate and standardize a tablet containing rosehip extract. To achieve this goal, a novel wet granulation method was employed in this study to develop a tablet formulation of rosehip extract. This method utilized rosehip concentrated extract as a granulation liquid, and double granulation was employed to optimize the content of the extract. Various formulations were systematically evaluated to determine the optimal composition and ratio of excipients. Subsequently, the final formulations underwent rigorous assessment of their physicochemical properties and stability. Rosehip extract and its tablets were standardized based on ascorbic acid and total polyphenol content, using HPLC and Folin-Ciocalteu methods accordingly. To the best of our knowledge, this is the first study to report the double-wet-granulation method in a tablet formulation design along with an examination of its impact on the total polyphenol content of the extract. The key advantage of employing this method lies in its capability to incorporate liquid extract into a solid formulation, thereby facilitating the accommodation of escalating dosages of extract in each tablet. However, it is important to note that this method does come with certain limitations. Primarily, the extended formulation process necessitates prolonged exposure of active ingredients to heat and oxygen, which may potentially affect their stability and efficacy.

Rosehip Extract Decreases Reactive Oxygen Species Production and Lipid Accumulation in Hypertrophic 3T3-L1 Adipocytes with the Modulation of Inflammatory State.

Rosa canina L. (rosehip) is used worldwide in traditional medicine as a plant with medicinal properties. However, its anti-obesity effects are not fully explained on a transcriptional level. In the present work, the 3T3-L preadipocytes were utilized to explore the impact of R. canina fruit extract (RCE) on the cellular and molecular pathways involved in adipocyte hypertrophy. Obtained results showed the ability of RCE to reduce lipid overloads in hypertrophic adipocytes associated with the down-regulation of mRNA expressions of adipogenic transcription factors such as PPARγ, C/EBPα, and SREBP-1c as well as genes involved in lipid biosyntheses such as FAS, LPL, and aP2. Moreover, obesity-associated oxidative stress (antioxidant enzyme activities and ROS generation) and inflammation were ameliorated in RCE-treated hypertrophic adipocytes. The mRNA and protein levels of adipokines such as leptin, resistin, and adiponectin were restored to more favorable levels. Rosa canina fruit might be a valuable source of phytochemicals in preventing obesity and obesity-related metabolic complications.

Identification of the effect of ripening conditions on the yield of rose hips and their processed products

Wild rose hip fruit, pulp, beverage, nectar, as well as technological methods and tools are taken as the object of research. Rose hips have a, bright red color and a rich chemical composition, which makes it a very important raw material for industry. However, insufficient research slows down this work and makes the use of raw materials inefficient. With this in mind, the aim was to study the mechanical and technological characteristics of the femur, which are considered important for industrial processing. The reflection of ripening conditions on the mechanical, physical and chemical properties of rose hips, as well as the influence of processing methods, especially hot processing, on the quality of the product has been established. Research has shown that the percentage of fruit cultivation in the Dashkasan region was the lowest, and the highest in the Gadabay region, and the Goygol region took an intermediate position. The Latin hardness of fruits grown in Dashkasan region was 6.41 N. Gadabay region with 5.56 N and Goygol region with 5.47 N. This indicator plays an important role in the storage and processing of fruits. As a result of the treatment, the amount of dry matter in fruit, pulp and beverage increased slightly, titratable acids, ascorbic acid and pH decreased. An increase in total phenolic compounds was observed during the initial heat treatment of the raw material and was noted to be much lower in the pulp and beverage. Hot treatment had a reducing effect on the amount of ascorbic acid, which is considered one of the main components of indicators in the thigh. The obtained results can be used in individual family farms and in the food industry

Rose Hip as a Possible Herbal Remedy for Weight Loss: A Systematic Review

Rose Hip as a Possible Herbal Remedy for Weight Loss: A Systematic Review

Enhancement of rosehip bioactive compounds by cold plasma pretreatment and application of its extract as a functional ingredient in ketchup

The effect of cold plasma (CP) was investigated on rosehip characterization for 1,2.5, and 5 min. All of the samples that were treated with CP had higher amounts of total phenolic content (TPC), antioxidant activity, vitamin C, and lycopene compared to the control (P < 0.05). The extract obtained by rosehip pretreated for 1 min had the highest antioxidant activity as well as bioactive compounds (except anthocyanin) and was selected for application in ketchup. Utilizing the CP-treated rosehip extract (RE) in ketchup successfully enhanced TPC (by 1.44 times), flavonoids (by 1.31 times), antioxidant activity (by 1.21 times), carotenoids (by 1.74 times), lycopene (by 1.11 times), vitamin C (by 1.6 times), and anthocyanins (by 2.46 times) compared to the control (P < 0.05). Moreover, the phenolic profile demonstrated that the highest increase belonged to catechin. Therefore, the RE pretreated by CP has the potential to develop a functional ketchup with high bioactive substances.

Biological and ecological characteristics of representatives of the genus Rosa L. (introduction, fruit characteristics, use)

In the steppe region an increase in the level of environmental pollution inevitably leads to a deterioration in the ecological condition of all components of ecosystems and leads to growth in the incidence of diseases among the population of the territory. It is possible to strengthen the natural immunity of the human body due to the introduction into the diet of products that contain biologically active compounds obtained from fruits, leaves, roots, etc. of representatives of the flora. Such plants include, first of all, representatives of the genus Rosa L. The high prophylactic and therapeutic effect of rose hips is confirmed by modern research by scientists. Among the vitamins that are part of the fruit, the highest content of vitamin C (ascorbic acid). Physiological and ecological studies of plants of the genus Rosa, in particular, indicators of success of introduction and vitamin C content, were carried out on the territory of the Botanical Garden of Oles Honchar Dnipro National University (DNU). It was found that due to their decorative properties and high-vitamin fruits, these plants are indispensable in the conditions of the steppe Dnieper region. Representatives of the genus Rosa are characterized by a variety of life forms and the specifics of the architectonics of the crown, the shape and color of the leaves and abundant flowering. It is shown that the climatic conditions of the new place of growth directly affect the growth and formation of shoots, and the annual cycle of plant development has its own characteristics, which are manifested during the growth of shoots, differentiation, the phase of physiological rest or forced dormancy depending on the geographical origin of species. Rose hips also differ in the size of fruits and seeds. Large fruit sizes and a significant number of seeds were observed both in the local species R. canina and in some introduced species of R. micrantha, R. iberica, R. hugoni. In the species R. multiflora and R. borboniana, small fruits and a small number of seeds were found. In this group of plants significant interest is associated with the high content of vitamin C in their fruits, which should not be destroyed during storage. As a result of our many years of research, it has been found that during fruit drying, there is a certain loss of ascorbic acid depending on the species characteristics of plants. The vitamin is best preserved in the fruits of R. rugosa (up to 58%) and R. hugonis (up to 55%). Other types are recommended to be used fresh. In addition, rose hips are highly decorative plants with long flowering and brightly colored fruits, which allows you to use this group of plants when creating compositions in modern landscape design.

Enhancing polyamide fabric performance through green reduction of graphene oxide for superior ultraviolet protection and electrical conductivity

AbstractIn this study, a polyamide warp‐knitted fabric was treated with a graphene oxide (GO) aqueous dispersion using a laboratory‐type Jigger dyeing machine. Subsequently, the GO underwent a chemical reduction process, employing rosehip extract powder as a nature‐based reducing agent. The effect of reduction time (12 and 24 h) was investigated. The study involved an examination of the surface morphology, colour coordinates, and colour difference of both the GO coated and reduced graphene oxide (RGO) coated fabrics. Additionally, the colour fastness against rubbing was assessed. Furthermore, electrical resistivity measurements were conducted on the GO‐coated polyamide‐6,6 fabric both before and after the reduction process. The ultraviolet (UV) transmittance and ultraviolet protection factor (UPF) of both GO‐coated and reduced fabrics were determined. The study also explored the effect of the washing process on colour properties, electrical resistivity, and UPF. Notably, the lowest electrical surface resistivity and the highest UPF rating were obtained as 1.20 × 102 kΩ/sq and 50+ after the reduction process. Additionally, after the washing process, the electrical surface resistivity increased, while the UPF rating remained 50+, indicating excellent UV protection.

Anti-Toxoplasma gondii activity of rose hip oil-solid lipid nanoparticles.

Toxoplasma gondii is a highly prevalent pathogen, reported from almost all geographical regions of the world. Current anti-T. gondii drugs are not effective enough in immunocompromised patients, encephalitis, chorioretinitis, and congenital toxoplasmosis. Therefore, the prescription of these drugs has been limited. Rose hip oil (RhO) is a natural plant compound, which shows antibacterial, anticancer, and anti-inflammatory activities. In the current study, the anti-T. gondii and cell toxicity effects of solid lipid nanoparticles (SLNs) loaded by RhO (RhO-SLNs) were evaluated. Emulsification sonicated-homogenization method was used to prepare SLNs. RhO-SLNs were characterized, and their anti-T. gondii and cell toxicity effects were evaluated using invitro analyses. The particle size and the zeta potential of the nanoparticles were 152.09 nm and -15.3 mV nm, respectively. The entrapment efficiency percentage was 79.1%. In the present study, the inhibitory concentration (IC)50 against T. gondii was >1 μg/mL (p-value <.0001). The cell toxicity assay showed cytotoxicity concentration (CC)50 >10 mg/mL (p-value = .017). In addition, at least 75% of T. gondii-infected Vero cells remained alive at concentrations >10 mg/mL. The concentration of 1 mg/mL showed highest anti-Toxoplasma activity and lowest cell toxicity against the Vero cell. Our findings suggest that carrying natural plant compounds with SLNs could be considered an effective option for treatment strategies against T. gondii infections.

Kuşburnu (Rosa canina L.) Tohumu Tozunun Mineral ve Biyoaktif Bileşen İçerikleri

This study determined the bioactive components of rosehip (Rosa canina L.) extract obtained via supercritical carbon dioxide extraction. The total phenolic content of its extract was 214.4 mg gallic acid equivalent/kg, with the total flavonoid content of 21.1 mg quercetin equivalent/kg. The antioxidant activity of the extract, which was determined by the DPPH (2,2-diphenyl-1-picrylhydrazyl) assay, was 64.8 μmol Trolox® equivalent antioxidant capacity (TEAC)/g. Gas chromatography-mass spectrometry identified 15 bioactive components in the extract. Additionally, pre- and post-processing heavy metal analyses were conducted on rosehip powder and seeds using inductively coupled plasma mass spectrometry (ICP-MS). Results showed that metal concentrations ranged from 0.064 to 9134.1 mg/kg in rosehip powder and from 0.143 to 1929.5 mg/kg in seeds, with the concentrations of potassium and magnesium as the highest. Despite the limited uses of rosehip products, this study indicated that wild rosehips are rich in functional components with potential health benefits.

Effects of Daily Intake of Rosehip Extract on Low-Density Lipoprotein Cholesterol and Blood Glucose Levels: A Systematic Review.

This systematic review evaluates the effects of the daily intake of rosehip extract on low-density lipoprotein cholesterol (LDL-C) and blood glucose levels. It synthesizes findings from randomized clinical trials focusing on cardiovascular and metabolic health outcomes. The review includes studies employing various forms of rosehip supplementation, assessing primary outcomes such as LDL-C, HDL-C, total cholesterol, triglycerides, fasting blood glucose (FBG), and glycated hemoglobin (HbA1c). Secondary outcomes, such as body weight, BMI, and blood pressure, are also considered. The paper discusses the potential mechanisms of rosehip's action, including modulation of peroxisome proliferator-activated receptors and effects on various metabolic pathways. The results indicate mixed effects on lipid profiles and blood glucose levels, with some studies showing significant benefits. This review underscores the need for further research to confirm optimal dosages, treatment durations, and rosehip's efficacy in diverse populations, considering its favorable safety profile. The findings suggest the potential of rosehip extract as a complementary agent in managing cardiometabolic risk factors.

FERMENTATION OF KVASS WORT BY INDUSTRIAL STRAINS OF MICROORGANISMS

We investigated the fermentation of kvass wort using yeast from the brewing industry and ready-made leaven of lactic acid bacteria. The raw materials for making kvass are kvass wort concentrate, sugar syrup, and rosehip extract. The content of dry substances in the initial wort is 3.2 wt. %, and pH = 5.8. Safale HA-18 dry yeast (optimal fermentation temperature is 25–35°C) and complexes of lactic acid bacteria in the form of starters "Kefir", "Yogurt", "Symbiotic", "Bifivit" of different microbiological compositions are used for kvass wort fermentation. We investigated the dynamics of the content of dry substances and the acidity under different fermentation conditions of kvass wort. The normalized value of the content of dry substances in kvass wort was obtained when using the "Bifivit" lactic acid bacteria starter at a temperature of 30°C, and the ratio of yeast, lactic acid bacteria in the starter is 50 % : 50 %. It is explained by the insufficient temperature necessary for the full reproduction of yeast and the impurity part of lactic acid bacteria. With an increase in the fermentation temperature of kvass to 35°C and a simultaneous increase in the proportion of yeast in the suspension, an increase in fermentation activity in all studied samples was observed. According to research, the optimal amount of yeast and lactic acid bacteria in the suspension is 4 wt. %. The optimal ratio of yeast in the mixture is 80 wt. % and lactic acid bacteria is 20 wt. %. According to the conditions of the experiment, norms of the content of dry substances (2.4 wt. %) and increased acidity (by 1.2 ml 0.1 N NaOH/100 ml) were achieved by samples No. 1 and No. 4 during 14–16 hours of fermentation. We suggested blending the prepared kvass with rosehip extract to give the drink additional functional and preventive properties. The blending of kvass with sugar syrup and rosehip extract was carried out to the content of dry substances 4.2 wt. % and a titrated acidity of 3 ml 0.1 N NaOH/100 ml. A comparative tasting evaluation of kvass with rosehip extract is given. Blending has an excellent effect on organoleptic indicators and gives the drink additional functional and preventive properties. The perspective of using industrial strains of microorganisms for the fermentation of kvass wort for small craft breweries is indicated

Comparative Study of Bioactive Compounds and Biological Activities of Five Rose Hip Species Grown in Sicily.

Nowadays, research on plant extracts has attracted increasing interest. The aim of this study was to compare phenolic profile, vitamin C, and carotenoid content, as well as the biological activities of five different rose species, including Rosa canina, R. corymbifera, R. micrantha, R. rubiginosa, and R. rugosa. These species had different morphological characteristics, with R. rugosa showing higher size of flower petals and higher weight of hips. The highest vitamin C content was found in hip extracts of R. rubiginosa and R. rugosa, which also showed the highest carotenoid amount. R. corymbifera showed the highest phenolic content. No significant antimicrobial activity of extracts containing phenolic compounds against different indicator strains could be detected. Cell monolayer integrity was not affected by treatments with the above-mentioned extracts of R. canina, R. micrantha, and R. rugosa at different concentrations for up to 24 h, while those of R. rubiginosa and R. corymbifera affected intestinal permeability at the highest concentration tested. The partial least squares regression analysis generated a predictive model correlating phenolic compounds with cell monolayer integrity, suggesting a relevant role for catechin, quercitrin, and p-coumaric acid. In conclusion, this study highlights how rose hips belonging to different species can have a diverse phenolic profile, differently influencing intestinal monolayer integrity.

Study of chemical compositions of wild and dry rose hip extract regarding the geographical zone of growth

The purpose of the study was a comparative study of the chemical compositions of wild and dry rosehip extract. The chemical composition of wild rose hips dried using the solar-air drying method was studied. The results of the analysis of the chemical composition showed that in wild rose hips the mass fraction of protein is 1.2 times less, ash and carbohydrates are 1.1 times less. The mass fraction of lipids in wild rose hips was 3-4%. Rose hips contain a higher amount of dietary fiber compared to dry rose hip extract. The ash content is 6.3 times, the protein is 1.7 times more, and the lipids are 20 times less in dry rose hip extract than in rose hips. The mineral composition of rose hips shows a high content of potassium and low sodium in wild rose hips, which is a positive factor in the prevention of atherosclerosis and hypertension. Wild rose hips contained 2.2 times more magnesium than varietal rose hips. The amount of vitamin C was 400 mg%, carotenoids 44 mg%. The total content of flavonoids in wild rose hips was 323 mg%. The study showed that during the solar-air drying process used in the Republic of Uzbekistan significant losses of vitamin C occur amounting to more than 50%.

Nickel oxide nanoparticles synthesized by Rose hip extract exert cytotoxicity against the HT-29 colon cancer cell line through the caspase-3/caspase-9/Bax pathway

Nickel oxide nanoparticles synthesized by Rose hip extract exert cytotoxicity against the HT-29 colon cancer cell line through the caspase-3/caspase-9/Bax pathway

Breeding of Modern Rose Cultivars Decreases the Content of Important Biochemical Compounds in Rose Hips.

This study aimed to determine the content and composition of bioactive compounds in autochthonous rose hips (R. pendulina, R. spinosissima, and R. gallica) and to compare them with the content of bioactive compounds in some cultivars ('Harstad', 'Bourgogne', 'Mount Everest', 'Poppius', 'Fruhlingsduft', 'Single Cherry', 'Fruhlingsmorgen', 'Violacea', and 'Splendens') derived from these main species. Due to insufficient information on how bioactive compound content changes when crossing roses, this study also sought to ascertain whether modern rose hip cultivars are still a sufficiently rich source of bioactive compounds and could, therefore, be potentially used as a functional food. All material was collected in the Arboretum Volčji Potok (Slovenia). The ascorbic acid content was highest in the 'Harstad' cultivar (12.79 g/kg FW), and the total organic acid content varied from 1.57 g/kg FW (R. spinosissima) to 34.39 g/kg FW ('Harstad'). Of all the carotenoids analyzed, only lycopene and β-carotene were present in all the samples. The total carotenoid content was highest in the 'Fruhlingsmorgen' cultivar (100.84 mg/kg FW), derived from R. spinosissima, and lowest in the main species, R. spinosissima (9.26 mg/kg FW). It can be concluded, therefore, that the content of bioactive compounds in rose hips of modern cultivars is generally lower than in rose hips of old cultivars and original species included in this study. The research results confirm that modern breeding strategies are mainly focused on goals such as abundant flowering and resistance to diseases and pests and not so much on the content of bioactive compounds.

Investigation of Physicochemical Properties and Surface Morphology of Hydrogel Materials Incorporating Rosehip Extract.

Hydrogel materials are used in many fields of science and industry. They are of particular importance in biomedical applications. In this work, hydrogels were obtained that could act as a dressing for wounds, at the same time being a carrier of substances with antioxidant activity. The discussed materials were obtained in the field of UV radiation. The correlation between the amount of photoinitiator used and the physicochemical properties and surface morphology of the obtained materials was investigated. In addition, the hydrogels have been incorporated with wild rose extract, which is characterized by antioxidant and anti-inflammatory effects. The analysis of the sorption capacity confirmed that the obtained material is able to absorb significant amounts of incubation fluids, which, in terms of application, will enable the absorption of exudate from the wound. The highest stability of materials was noted for hydrogels obtained with the use of intermediate amounts of photoinitiator, i.e., 50 µL and 70 µL. In the case of using 20 µL or 100 µL, the photopolymerization process did not proceed properly and the obtained material was characterized by a lack of homogeneity and high brittleness. With the increase in the amount of photoinitiator, an increase in the surface roughness of hydrogel materials was confirmed. In turn, spectroscopic analysis ruled out the degradation of materials in incubation fluids, indicating the potential for their use in biomedical applications.

Rosehip Extract-Loaded Liposomes for Potential Skin Application: Physicochemical Properties of Non- and UV-Irradiated Liposomes.

In the present study, rosehip (Rosa canina L.) extract was successfully encapsulated in phospholipid liposomes using a single-step procedure named the proliposome method. Part of the obtained liposomes was subjected to UV irradiation and non-treated (native) and UV-irradiated liposomes were further characterized in terms of encapsulation efficiency, chemical composition (HPLC analysis), antioxidant capacity, particle size, PDI, zeta potential, conductivity, mobility, and antioxidant capacity. Raman spectroscopy as well as DSC analysis were applied to evaluate the influence of UV irradiation on the physicochemical properties of liposomes. The encapsulation efficiency of extract-loaded liposomes was higher than 90%; the average size was 251.5 nm; the zeta potential was -22.4 mV; and the conductivity was found to be 0.007 mS/cm. UV irradiation did not cause a change in the mentioned parameters. In addition, irradiation did not affect the antioxidant potential of the liposome-extract system. Raman spectroscopy indicated that the extract was completely covered by the lipid membrane during liposome entrapment, and the peroxidation process was minimized by the presence of rosehip extract in liposomes. These results may guide the potential application of rosehip extract-loaded liposomes in the food, pharmaceutical, or cosmetic industries, particularly when liposomal sterilization is needed.

Rosehip extract and wound healing: A review.

Each year, over 100 million patients are afflicted with new scars from medical procedures worldwide. Natural compounds have shown promise in the treatment of scars and skin disorders. Rosehip oil (RO), produced from the pressed fruit of the rosehip (Rosa canina L.) plant, is used in the pharmaceutical, cosmetic, and food industries. The use of this plant in the treatment of scars has yet to be reviewed. This review aims to analyze the current findings on the use of RO in the treatment of postsurgical scars. This literature search considered published journal articles (clinical trials or literature reviews). Studies were identified by searching electronic databases (PubMed and MEDLINE) and reference lists of respective articles. Additional articles were identified through Google Scholar. Only articles available in English were included in this review. There is a scarcity of high-quality studies assessing the therapeutic potential of RO. From the two human clinical trials using RO, there is some evidence to suggest its potential as an active ingredient in topical formulations for the treatment of wounds. Topical treatments containing RO extract may reduce the size and erythema of postsurgical scars through the polarization of macrophages and the inhibition of inflammatory cytokines. Some evidence suggests that RO may improve postsurgical scars. At present, there is insufficient evidence to recommend the use of RO for the treatment of wounds. Further investigation is required to establish its therapeutic effects on human skin and its potential use as an ingredient in topical formulations.