Rooting Of Cuttings Research Articles

Improvement of the elements of the technology of growing black currant seedlings

In recent years, the fruit growing industry has undergone significant changes, for example, over the past 10 years, domestic production of fruit and berry products has increased by more than 40%, the lion’s share of which is used in the food and processing industry of the Russian Federation, and occupy a special position there. Fruit and berry products are the most important source of vitamins, including vitamins such as C, P, E, K and carotene, which are not synthesized in our body in any way and must be supplied regularly with food. However, with the growth of production of these products, the need for planting material is also growing, as well as the cost of manufacturing the products themselves. In this regard, the low use of intensification agents in fruit growing, including the use of humic preparations and root-forming agents, significantly reduces the efficiency of production of high-quality planting material. To solve this problem and reduce the cost of producing seedlings by the method of lignified cuttings, it is necessary to use intensification techniques using organic fertilizers based on local raw materials. The research was carried out in 2019–2021 at the site of lignified cuttings of berry crops, the educational and experimental farm “Michurintsev Garden” of the Novosibirsk State Agrarian University. The paper analyzes the features of propagation of garden crops by the method of lignified cuttings. The effect of organic fertilizers on the survival, rooting and overwintering of cuttings is shown. The features of the growth and development of black currant under the action of organic fertilizers have been studied. The yield of commercial seedlings of garden crops has been established under the influence of applied technologies.

ОСОБЕННОСТИ ВЕГЕТАТИВНОГО РАЗМНОЖЕНИЯ НЕКОТОРЫХ ПРЕДСТАВИТЕЛЕЙ РОДА CHRYSANTHEMUM L.

The purpose of research is to study the features of vegetative propagation by green cuttings of some varieties of small-flowered chrysanthemums. Tasks: determination of the coefficient of vegetative multiplication (CVR) and the degree of rooting of green cuttings, selection of varieties for industrial cultivation. The objects of study are 92 taxa of small-flowered chrysanthemum from the collection of the South Ural Botanical Garden-Institute of the Ural Federal Research Center of the Russian Academy of Sciences. The study was carried out in 2016–2022 on the basis of the laboratory of introduction and selection of flowering plants in a production greenhouse. Statistical processing of the data array was carried out using the MS Excel and Statistica 10 software packages. Three groups of varieties were identified in terms of CVR: difficult to propagate, or unproductive – CVR < 10 (‘Zolotoj Orfej’, ‘Svemba Kars’, ‘Elen’, etc.), medium productive – CVR 10–19 (‘Vechernie Ogni’, ‘Izabel’, ‘Kurochka Ryaba’, etc.), easily propagated, or highly productive, – CVR ≥ 20 (‘Varvara’, ‘Director Z.Kh. Shigapov’, ‘Professor L.M. Abramova’, etc.). Also, 3 groups of varieties were identified according to the rooting rate of green cuttings: with a low degree – < 60 % (‘Valli Ruf’, ‘Vi Villi’, ‘Listopad’, etc.), with an average degree – 60–85 % (‘Dina’, ‘Zlata, ‘Cygan, etc.), with a high degree of rooting – > 85 % (‘Volny Agideli’, ‘Doktor V.P. Putenihin’, ‘Mazurka’, etc.). Based on the totality of the studied parameters, 19 varieties were identified that can be recommended for industrial use.

Investigation of the Effects of Rooting Medium and IBA Concentration on Rooting and Shoot Development of Bougainvillea (Bougainvillea spp.) Cuttings

In this study, the effect of different rooting mediums (peat moss, peat + sand, peat + loam) and IBA (indole-3-butyric acid) concentration was investigated on hardwood cuttings taken from Bougainvillea glabra, Bougainvillea spectabilis, and Bougainvillea x buttiana species. The cuttings were treated with 0, 1000, 2000 and 3000 mg/l IBA. Statistical evaluation was performed according to a randomized block experimental design with three factors and three repetitions. The results showed that Bougainvillea x buttiana cuttings had the highest number of roots, root fresh weight, longest shoot length, and shoot fresh weight. Cuttings grown in 100% peat medium had the highest rooting percentage, root number, root dry weight, leaf number, and shoot fresh weight. Regarding IBA concentrations, the cuttings treated with 3000 ppm IBA had the highest number of roots, root length, and root fresh weight. Furthermore, in all of the Bougainvillea species, the highest rooting percentage and leaf number were observed in cuttings treated with 2000 ppm IBA. Notably, Bougainvillea x buttiana cuttings treated with 1000 ppm IBA dose and planted in peat medium provided the longest shoot length, highest shoot fresh weight, and shoot dry weight. In conclusion, the findings show that the growing medium has a statistically significant effect on the rooting and root quality of cuttings and that it would be useful to investigate various rooting hormones, dasages and rooting media to increase rooting and shoot development. Considering growing medium plays a crucial role in plant growth, it can be stated that further improvements in rooting and growth can be realized via utilizing the enhanced physical and chemical properties of peat-based mixtures.

Effect of cutting origin, season of collection, and treatment on rooting of shoot-tip cuttings of Salvia tomentosa Mill.

Effect of cutting origin, season of collection, and treatment on rooting of shoot-tip cuttings of <i>Salvia tomentosa</i> Mill.

Involvement of endogenous IAA and ABA in the regulation of arbuscular mycorrhizal fungus on rooting of tea plant (Camellia sinensis L.) cuttings

BackgroundAdventitious root (AR) formation is the key step for successful cutting propagation of tea plants (Camellia sinensis L.). Studies showed that arbuscular mycorrhizal fungus (AMF) can promote the rooting ability, and auxin pathway in basal stem of cuttings was involved in this process. However, auxin and abscisic acid (another important regulator on AR formation) in the other parts of cuttings at different rooting stages responding to AMF inoculation are not well studied. Therefore, in this paper, contents, enzymes and genes related to these two plant hormones were comprehensively determined aiming to unveil how endogenous indole-3-acetic acid (IAA) and abscisic acid (ABA) involve in the AMF regulating AR development of tea cuttings.ResultsInoculating with AMF significantly increased the proportion of cuttings at S2 stage (AR formation), which was more than twice as much as the control. And the total rooting rate in mycorrhizal treatment was also higher than that in the control with an increase of 8.66%. Enzyme activity assays showed that except for decreased polyphenol oxidase (PPO) activity at the S3 stage and peroxidase (POD) activity in middle stem of S3 stage, AMF inoculation increased activities of POD, PPO, superoxide dismutase (SOD) and catalase (CAT) to varying degrees in leaf, middle stem and basal stem of tea cuttings. After inoculation with AMF, the indoleacetic acid oxidase (IAAO) activity decreased to a certain extent in the first three stages of tea cuttings, which showed a trend of ‘low-high-low’ in the basal stem of all treatments. Besides, there was a significantly positive correlation between SOD activity and AR formation, especially for the proportion of cuttings at S2 and S3 stages. Higher IAA level and IAA/ABA ratio was found in basal stem of cuttings at S1 stage induced by AMF, which promoting the AR formation as revealed by correlation analysis. At the same time, AMF significantly elevated the level of IAA in leaf at S1 stage. By screening differentially expressed genes (DEGs) related to IAA and ABA pathways, together with redundant analysis, it was indicated that auxin biosynthesis and transport, as well as ABA transport and signal transduction, were involved in AMF regulating the rooting of tea cuttings.ConclusionsOverall, both endogenous IAA and ABA played roles in the regulation of AR formation of tea cuttings by AMF inoculating, enriching the theoretical basis of AMF regulating rooting of cuttings and providing foundations for cutting propagation of tea plants.

Effects of nutrient solution concentration during the nursery period on the growth of Stevia cuttings in hydroponics

The effects of nutrient solution concentration on the growth of stevia cuttings during the nursery period in hydroponics were investigated. Cuttings were inserted in continuously aerated water, nutrient solution of 0.3 dS m-1, and nutrient solution of 0.7 dS m-1. Rooting of cuttings began on the 10 days after cutting. The rooting rate of cuttings under water and EC 0.3 dS m-1 reached 100% on day 13. The nutrient solution concentration during the nursery period did not affects rooting rate. At transplanting, best stem length and number of leaves were observed 0.7 dS m-1. The maximum root length was not affected nutrient solution concentration. At harvest, the shoot dry weight of 0.7 dS m-1 was significantly heavier than that of water. Survival rates after transplanting of 0.3 dS m-1 and 0.7 dS m-1 were lower than that of water. Despite some issues with the survival rate, the use of a nutrient solution during the nursery period in hydroponics of stevia could lead to more efficient production.

Functional Analysis of CLE26 in Controlling De Novo Root Regeneration from Detached Arabidopsis Leaves.

De novo root regeneration is the process by which adventitious roots form around the wound site from wounded or detached plant organs. The de novo root regeneration process has been widely exploited in cutting technology used for vegetative propagation. Here, we employed detached leaf explants from Arabidopsis thaliana to form adventitious roots for studying the process of de novo root regeneration. GUS staining showed that the expression of CLAVATA3/EMBRYO SURROUNDING REGION-RELATED26(CLE26) was gradually increased surrounding the wound site of leaf explants during adventitious root formation. Semi-thin sections further showed that the expression pattern of CLE26 was closely linked to the formation of adventitious roots. Next, genetic analyses confirmed that the CLE26 gene was involved in de novo root regeneration. Furthermore, RNA sequencing (RNA-seq) of the leaf explants revealed that stress-related genes might be involved in CLE26-mediated adventitious root formation. Specifically, genes associated with the hydrogen peroxide catabolic process and oxidative stress response were predominantly upregulated in the cle26 mutant. In contrast, genes involved in the response to salicylic acid were largely downregulated in the cle26 mutant. Overall, our study indicates that the mutation in CLE26 might upregulate the expression of genes involved in reactive oxygen species metabolism or suppress the expression of genes associated with salicylic acid synthesis, thus promoting the formation of adventitious roots. These findings suggest that CLE26 is a potential candidate for the genetic improvement of adventitious rooting in cuttings.

Photosynthetic Daily Light Integral Effects on Rooting and Vegetative Growth of Cuttings of Six Foliage Plants

Recently, millennials, social media, and the COVID-19 pandemic have spurred the demand for indoor foliage plants. Between 2019 and 2022, the wholesale value of foliage plants in the United States increased by 45% to $1 billion. However, few propagation protocols are available for greenhouse growers in temperate climates. Additionally, the mechanisms by which shade and succulent crops photosynthesize, root, and accumulate biomass in response to environmental parameters are largely unknown. Therefore, the objective of this study was to determine the photosynthetic daily light integral (DLI) that leads to uniform, high-quality, and rapid rooting of foliage crop cuttings. Vegetative shoot tip cuttings of Curio peregrinus ‘String of Thai Dolphins’ (dolphin plant), Curio rowleyanus ‘String of Pearls’ (string-of-pearls), Curio herreianus ‘String of Pearls Big Bead’ (string-of-pearls big bead), Peperomia obtusifolia ‘Emerald Isle’ (baby rubber plant), Peperomia ferreyrae ‘Happy Bean’ (pincushion peperomia), and Sedum makinoi ‘Tornado’ (stonecrop) were excised from stock plants. Then, the cuttings were inserted in plug trays filled with a propagation medium consisting of a 1:1 (v/v) of half 70% peatmoss, 21% perlite, and 9% vermiculite and half medium perlite. Then, the cuttings were placed under a DLI of approximately 5 mol·m−2·d−1 for 7 days of callus induction, and subsequently under shading of 70% (2.9–3.4 mol·m−2·d−1), 50% (4.5–6.5 mol·m−2·d−1), 30% (7.8–11.2 mol·m−2·d−1), or 0% (10.2–16.4 mol·m−2·d−1), resulting in DLIs ranging from 2.9 to 16.4 mol·m−2·d−1. After 21 days, the stem length of stonecrop and pincushion peperomia rooted cuttings decreased as the DLI increased from 2.9 to 16.4 mol·m−2·d−1. However, the stem length of string-of-pearls and string-of-pearls big beads rooted cuttings increased after 28 days as the DLI increased from 2.9 to 16.4 mol·m−2·d−1. As the DLI increased from 2.9 to 16.4 mol·m−2·d−1, the shoot dry mass of dolphin plant and baby rubber plant significantly increased by 168% and 189%, respectively. Additionally, the root dry mass of dolphin plant and baby rubber plant significantly increased by 376% and 50%, respectively. The quality index of all five cultivars was greatest under DLIs of 10.2 to 16.4 mol·m−2·d−1, with the exception of pincushion peperomia, which had the highest quality index under DLIs of 2.9 to 4.6 mol·m−2·d−1. Our findings indicate that higher DLIs benefit shoot growth during rooting, but not necessarily root biomass accumulation of all genera investigated.

Evaluating Paeonia emodi propagation protocols by seed and root cuttings and phytochemical analysis of the plants

The present study aimed to develop conventional propagation protocols for Paeonia emodi, a threatened and high-value medicinal herb of the Himalayan region. Different experiments were conducted to evaluate the effects of various concentrations of plant growth regulators (PGRs) on seed germination and vegetative propagation (root cuttings) in nursery conditions. Leaves of well-developed plants were used to evaluate phytochemical content and antioxidant activity. Seed germination percentage reached a maximum (78.88 ± 1.25%) with gibberellic acid + abscisic acid treatment (GA3+ABA) at a concentration of 100 µM each. However, 100% sprouting was observed in roots treated with 500 mg L−1 indole-3-butyric acid (IBA). Seeds treated with GA3 (100 µM) induced a maximum plant height (11.98 ± 0.05 cm). Similarly, roots treated with IBA (500 mg L−1) resulted in 35.54 ± 0.84 cm plant height and maximum root diameter (25.18 ± 0.90 mm) in IBA (100 mg L−1) treated plants. Among differently treated plants, total phenolic content (100.95 ± 1.36 mg GAE/g dw) and 2, 2′-azinobis-3-ethylbenzothiazoline-6-sulfonate assay activity (25.24 ± 0.35 mg AAE/g dw) were higher in GA3+ABA (100 µM each) treated seed raised plants. However, vegetatively propagated plants treated with IBA 100 mg L−1 had the highest levels of total phenolics (110.42 ± 3.42 mg GAE/g dw) and 2, 2′-diphenyl-1-picrylhydrazyl assay activity (22.35 ± 0.48 mg AAE/g dw). The results of the study will help in the mass multiplication of the species for large-scale production.

Influence of &lt;i&gt;Ascophyllum nodosum&lt;/i&gt; extract on rooting of &lt;i&gt;Mentha spicata&lt;/i&gt; L.

Introduction. The production of medicinal plants is a market that has been growing rapidly in recent years and requiressustainable agronomic techniques to meet this demand. The herb spearmint (Mentha spicata L.) is one of these plants in demand worldwide. However, there are not many techniques for its commercial cultivation, whether in terms of harvest, post-harvest, or propagation. As for propagation, this is usually done vegetatively, occasionally with the use rooting agents. Among the wide variety of rooting agents available in the market, the use of algae extracts such as Ascophyllum nodosum is a sustainable alternative. Objective. To evaluate the influence of Ascophyllum nodosum extract on the rooting of spearmint cuttings. Materials and methods. The trial was carried out under greenhouse conditions at National Agricultural University La Molina, Peru with different doses of the extract and different evaluation times. Doses of 0, 1, 5 and 10 mL L-1 of the extract and 3 evaluation moments were used, which were 21, 28 and 35 days after cutting. In this way, an appropriate dose and transplanting time would be obtained. The variables evaluated were root number, root length, rooting percentage, fresh weight of stem, root and leaf, and dry weight of stem, root and leaf. Results. Spearmint cuttings showed significant differences (a = 0.05) for root length, rooting percentage, and root fresh weight with the application of the extract, with better results in the 10 mL L-1 dose for root length and root fresh weight. Conclusion. Ascophyllum nodosum extract proved to be efficient in rooting Mentha spicata cuttings.

Influence of Endogenous carbohydrate content on Initiation of adventitious roots in stem cuttings of Coleus blumei L.

Several internal and externally administered substances may stimulate or prevent the development of adventitious roots. The present study was conducted to analyze the role of endogenous carbohydrate content on the initiation of adventitious roots during the culture of Coleus blumei L. stem cuttings. The stem cuttings of the plant were cultured at various inclinations viz., 22.50, 450, 67.50, 900 and 1800 to the soil surface. The endogenous carbohydrate content in water was analyzed from stem cuttings (4cm length) on the initial day and after 4 days of culture of Coleus blumei L cuttings. At the base of the cultured cuttings, up to the 2 cm area of root initiation, the carbohydrate (glucose) amount exhibited variations along with inclinations. Additionally, a substantial relationship was found between the gravity component and the endogenous glucose level. Although the maximum amount of carbohydrate accumulation (325µg/g F.W.) was recorded at a 22.50 inclination, its subsequent accumulation at 450 inclination (256µg/g F.W.) exhibited a fast genesis of adventitious roots. This presumably indicated that the components of gravity (g), viz. pgsinθ and pgcosθ had a significant influence on the rate of transportation of endogenous carbohydrate content to the basal terminal part of the cultured cut sections that are maintained at varied inclinations. Accumulating carbohydrates at the base of cultured cuttings influences the development and maturation of adventitious roots in stem cut sections. Nonetheless, the research showed that the beginning of the rooting process in stem cuttings of Coleus blumei L. necessitates a certain quantity of endogenous carbohydrates.

Large deformation simulation of uprooting of trees with complex root system architectures using material point method with embedded truss elements

Abstract Background and aims Urban trees in coastal cities like Hong Kong may suffer from an uprooting failure when subjected to extreme winds. A proper numerical model for tree uprooting simulation can help to select tree species or soil types that better resist uprooting failure. However, modeling tree uprooting is challenging as it is a cross-disciplinary problem involving complex root system architectures (RSAs) and large deformation of both roots and soils. This study aims to develop a hybrid numerical model that combines truss elements and material point method (MPM) to simulate the entire large-deformation uprooting process of trees with complex RSAs. Methods The tree uprooting model is developed by coupling truss elements in finite element method (FEM) with MPM. Laboratory pull-out tests using artificial roots and real root cuttings are adopted to validate the developed model. A comparative study is performed to investigate the difference between using complex and simplified RSAs in tree uprooting simulations. Results The developed model provides consistent predictions of peak load, critical displacement and failure mode when compared with results from laboratory tests. Moreover, the comparative study shows that the uprooting resistance obtained with a complex RSA is higher than that with a simplified RSA. The difference varies with the soil and root mechanical properties. Conclusion The developed hybrid model offers a novel way for simulating an entire tree uprooting process involving large deformations and complex RSAs. The study shows that using a simplified RSA to approximate the complex RSA might result in misleading failure modes.

Biochemical and physiological effects of propagule type and auxin concentration on adventitious root formation in novel Jasmine genotypes

Jasmine is primarily propagated through asexual methods, and bio-stimulants can improve soil fertility around plant roots. Some jasmine genotypes are mainly propagated through cuttings; however, the rooting process is slow and inconsistent. Specific jasmine genotypes are primarily propagated through cuttings, but the rooting process is slow and uneven. This study evaluated the regeneration potential of stem cuttings by investigating the effects of auxins and various propagule types on adventitious root formation, along with associated biochemical changes. A field experiment conducted by the Department of Floriculture and Landscaping at TNAU, Coimbatore, Tamil Nadu, from December 2023 to June 2024, during the winter monsoon, investigated the rooting of stem cuttings in three Jasminum genotypes using auxin. Stem cuttings of J. sambac Double Flower type (DF), J. grandiflorum White Flower type (WF) and a new cultivar of J. multiflorum CO.1 Winter Jasmine (CO.1 WJ) were treated with three Indole-3-Butyric Acid (IBA) concentrations (0.5, 1, 1.5 g L-1) and distilled water as control. Utilizing a Factorial, Completely Randomized Design for terminal and semi-hardwood cuttings, the study found that rooting hormone significantly enhanced root formation and stem and shoot growth. Semi-hardwood cuttings of J. sambac (DF) treated with 1g L-1 IBA had the highest rooting rate (88.60%), number of roots (9.34), root length (12.74 cm), shoot length (10.26 cm) and number of leaves (6.60). The highest rooting rate of 48.15% was obtained from the terminal cuttings of J. grandiflorum (WF) that had been treated with 1g L-1 IBA, in addition to other parameters such as number of roots (13.52), root length (12.03 cm), shoot length (14.30 cm), and number of leaves (13.48). On the other hand, J. multiflorum (CO.1 WJ) recorded the highest rooting rate of 72.23%, root number of 16.52, root length of 17.22 cm, shoot length of 14.14 cm and number of leaves, which was pegged at 31.70 when terminal cuttings were treated with 1g L-1 IBA. The current findings indicate that the application of auxins is crucial for promoting early root initiation and achieving higher rooting success, making it advantageous for vegetative propagation.

Effect of Various Preparations for Plant Rooting on Olive Cuttings

The effect of different rooting agents on the propagation of olive plant by means of cuttings was investigated in a comparative manner. Experimental research works were carried out in the greenhouse complex of the Absheron Experimental Station. Olive plant cuttings from different varieties were taken as the object of the study. Rooters (IST and naphthenic acids) were used to influence the rapid rooting of cuttings and the increase in the number of lateral roots. During the conducted experiments, different results were obtained in different variants. It was observed that indolyl acetic acid and naphthenic acid were more effective on olive cuttings in comparison with the control option. Based on the conducted studies, it was found that rooting of cuttings was accelerated as a result of the effect of rooters. Results were more successful in the indolyl acetic acid variant than in the other variants. So, under the influence of 100 cuttings IST, 96 cuttings in Agbaba variety, 87 in Jigirina variety, 93 in Pikvales variety, and 89 cuttings in Azerbaijani olive were observed. Under the influence of naphthenic acid, rooting occurred in 92 of 100 cuttings of Agbaba variety, 87 of Jigirina variety, 85 of Pikwales variety, and 91 of Azerbaijani olives. As a result, it was determined that for the rapid development of the olive plant, cuttings should be taken from healthy and well-nourished trees and indolyl acetic acid should be used as a rooting solution.

Influence of IBA and NAA on Rooting of Terminal Cuttings of Chrysanthemum (Dendranthema grandiflora L.)

The present investigation was conducted at the Agricultural College and Research Institute, Vazhavachanur, Tiruvannamalai to study the effect of Indole-3-Butyric Acid (IBA) and Naphthalene Acetic Acid (NAA) on the rooting of terminal cuttings of chrysanthemum. An application of growth regulators (IBA and NAA) had a positive effect on the rooting of chrysanthemum terminal cuttings. The result of the experiment revealed that the treatment of IBA @ 400ppm significantly increased rooting percentage (89%), length of roots (5.67 cm), root spread (39.18 cm), number of roots (22.67), fresh weight of roots (4.94 g), plantlet weight (1.17 g), and plant height (8.87 cm) followed by NAA @ 400 ppm (79.33%), while minimum rooting percentage (47) was observed in control. It can be concluded that the treatment of IBA @ 400ppm significantly increased rooting percentage, roots length, number of roots, plantlet weight and plant height has greater survival percentage in the terminal cuttings of chrysanthemum (Dendranthema grandiflora).

Effects of Photoperiod Treatments on Stock Plants and Cutting Rooting of Winecups

Production of attractive and water-efficient plants native to the plains and prairies of central North America can sometimes be limited because of problems associated with successful rooting of vegetative cuttings. Winecups (Callirhoe involucrata) is an attractive native plant, valued for its long period of bloom, drought tolerance, and winterhardiness, but can be difficult to propagate from seeds and vegetative cuttings. Seed dormancy issues can result in unreliable germination and seedling variations, which reduces crop uniformity. Although propagation from rooted cuttings maintains crop uniformity, cuttings often fail to root or root poorly. Manipulating the growing conditions of stock plants to suppress reproductive growth may improve rooting success of vegetative cuttings. Based on research conducted earlier with other ornamental perennials and programmed photoperiods, winecups plants were grown under three programmed photoperiods to determine if a particular photoperiod could be used to suppress reproductive growth and promote vegetative growth. The study consisted of three experiments, all conducted in similar fashion but at different times during the year. Results indicate that plants grown under 10-hour and 8-hour photoperiods remained vegetative longer when compared with plants grown under 12-hour photoperiod. Vegetative cuttings harvested from plants grown under 8-hour photoperiod had higher percent rooting when compared with vegetative cuttings harvested from plants grown under 10-hour and 12-hour photoperiods. Based on the findings from this research, plant propagators may be able to increase production of winecups by growing stock plants under 8-hour photoperiod.

Studies on the effect of putrescine and different rooting substrates on rooting and survival of hardwood cuttings of fig (Ficus carica L.) cv. brown turkey under controlled conditions

Studies on the effect of putrescine and different rooting substrates on rooting and survival of hardwood cuttings of fig (Ficus carica L.) cv. brown turkey under controlled conditions

Vegetative Propagation of Wild Prometheum sempervivoides (Fischer Ex M. Bieb.) H. Ohba by Leaf Cutting: Effects of Auxin and Some Substances

The aim of the research is to determine the effects of different rooting contents [control (water), plant growth regulator-rooting hormone (H1: 0.25 g l-1, H2: 0.50 g l-1 and H3: 1.0 g l-1), cinnamon powder (T) and Aloe vera gel (AV)] and two different planting times on the rooting of leaf cuttings prepared from the stock mother plants of wild Prometheum sempervivoides (Fisch. ex Bieb.) H. Ohba taxon. Observations and measurements of vitality rate (%), number of rooted cuttings (NR), number of callusing cuttings (NC), maximum root length (MRL), rooting rate (%) and rooting scale (1-5) were made on cuttings whose rooting was completed. The results were evaluated statistically. The most positive rooting effect was determined in the H3 application, which is the highest dose of the commercial rooting hormone tested in the study. Cinnamon powder application was determined as a more effective natural extract for rooting leaf cuttings of P. sempervivoides when compared to Aloe vera gel application. At the end of our study, in which the effects of different planting times were also examined, it was determined that planting the leaf cuttings of P. sempervivoides as soon as they were taken from the stock mother plant had a positive effect on rooting. The very good quality roots were observed in H1 (0 h), H2 (0 h), H3 (0 h) and H3 (24 h) applications. These applications can be recommended for rooting the leaf cuttings of P. sempervivoides succulent plant. It was also concluded that P. sempervivoides can be reproduced by vegetative propagation method using leaf cuttings.

Effect of Plant Growth Substances on Rhizogenesis of Stem Cuttings in Pointed Gourd (Trichosanthes dioica Roxb.)

An experiment was conducted to know the influence of growth regulators IBA, NAA and salicylic acid with different concentrations on rooting of stem cuttings in pointed gourd (Trichosanthes dioica Roxb.) in College of Horticulture, Venkataramannagudem, during the year 2023-2024. The experiment was laid out by following Factorial Randomized Block Design (FRBD) with twenty treatment combinations which consists of two factors, Factor-1: types of cuttings (male and female) and Factor-2: growth substances (10). By adopting standard methodology, observations on root and shoot growth were recorded and statistically analyzed. Studies on the effects of various growth substances on the formation of roots in cuttings of pointed gourd vines showed that IBA at 150 ppm recorded highest percentage of rooting, the longest root, the number of roots per cutting the number of leaves per cutting, the length of the shoot and the percentage of establishment in main field among various growth substances. In comparison to female cuttings, male cuttings showed a considerably higher percentage of rooting, as well as more roots per cutting, longest root, longest shoot, more leaves per cutting and a higher percentage of establishment in the main field. IBA 150 ppm + male cuttings recorded considerably more roots per cutting and the longest root than the other combinations, according to the interaction between growth substance and type of cutting.

Rooting of softwood cuttings of ‘Porotan’ Japanese chestnut

Rooting of softwood cuttings of ‘Porotan’ Japanese chestnut