Aeration plays a crucial role in maintaining the overall health and sustainability of the recirculating aquaculture system. Conventional aeration operates continuous and consumes 35.06% of equipment energy consumption. How to optimize aeration while ensuring normal fish growth remains a great challenge. To address these challenges, this research paper established a coupled model of computational fluid dynamics and species transport model (CFD-STM). Aeration tests were carried out in a 33.3 m3 culture tank using a 0.31m diameter aerator to find out the impacts of operating variables (aeration flow rate, bubble diameter, inlet velocity, and aerator position) on oxygen transfer using a microporous aeration system. A CFD-STM model was coupled to obtain the optimum values of aeration parameters. Results revealed that the Standard Aeration Efficiency (SAE) reductions by 32.1% for 50% larger aeration flow rate and 86.4% for 50% larger bubble diameter, as well as SAE achieved 5.67kg/kWh with an aerator position scale factor equal to 0.48. In addition, the energy consumption of the Roots blower was compared for a single aeration and dissolved oxygen saturation. Results indicated that smaller bubbles microbubbles can effectively improve oxygen transfer coefficient. The energy consumption was reduced by 15.7% for 50% larger aeration flow rate and 38.6% for 50% lower bubble diameter. Based on the results, this study demonstrated that CFD is a useful tool to optimize the aeration system design and operation in order to enhance aeration efficiency. It provides a theoretical foundation the future optimization of energy conservation through intermittent aeration practices.