Root Potentials Research Articles

Eco‐Friendly Extraction and Utilization of Agro Crop Wastes Based Natural Dye for Textile Dyeing

ABSTRACTThe incorporation of waste plant residues into practical applications, particularly as a sustainable source of green dyes in textiles, is increasingly recommended by the global community. This research investigates the potential of Rangoon creeper flowers and madder roots for silk dyeing through environmentally friendly methodologies. Therefore, extraction procedures were conducted in suitable mediums and applied to the fabric before and after microwave (MW) treatment for durations of up to 10 min. Similarly, response surface methodology was employed to assess the significance of various dyeing parameters, which influence shade development and enhance colorfastness. The results indicate that subjecting acidic binary extracts of Rangoon creeper flowers and madder roots, along with silk fabric, to 6 min of radiation at 700 W is an effective condition for achieving colorfast shades, particularly when applied before and after mordanting with Al, Fe salts, and tannic acid single and their binary solution as eco‐chemical agents. The highest color strength (K/S = 15.0) was obtained using an acidic extract after MW treatment. Hence, evaluation based on standard methods such as ISO protocols for lightfastness, wash fastness, and rub fastness demonstrates that employing selected shades produced under environmentally friendly conditions is both time and energy‐efficient, yielding stable colorfast hues rated from good to excellent. This study suggests that utilizing microwave treatment in addition to statistical methodologies like the central composite design for exploring novel dye‐yielding plants, coupled with eco‐mordanting techniques, holds promise for obtaining desirable colorfast shades.

Identification and Diversity of Mycorrhizae in Several Rhizospheres of Plant Vegetation in Ultisol Land

The poor fertility of ultisol soil hinders the cultivation of food, horticultural crops, and plantation crop nurseries, leading to the abandonment of many lands. Low fertility and productivity levels and high solubility of Al, Mn, and Fe characterize ultisol soil. One effective method to enhance soil properties is by utilizing arbuscular mycorrhiza. Arbuscular Mycorrhizal Fungi (AMF) play a crucial role in improving nutrient and water absorption, thereby enhancing the growth potential of AMF-infected roots and plants. This study aims to identify the types and diversity of mycorrhizae present in the rhizosphere of plants growing in Ultisol soil. The study employed an exploratory approach involving the collection of soil and plant samples, analysing soil, and identifying Arbuscular Mycorrhizal Fungi (AMF) in Ultisol vegetation. The results revealed the presence of AMF in the rhizospheres of various plant species, including Seduduk, Harendong, Kirinyuh, Alang-Alang, and Paku Resam. Three mycorrhizal genera were identified, namely Glomus, Acaulospora, and Gigaspora.

Comparative phenolic profile and antioxidant potential of mentha hairy roots and aerial parts

Comparative phenolic profile and antioxidant potential of mentha hairy roots and aerial parts

Deciphering the Phytochemical Potential of Hemp Hairy Roots: A Promising Source of Cannabisins and Triterpenes as Bioactive Compounds.

Cannabis sativa L., specifically hemp, is a traditional herbaceous plant with industrial and medicinal uses. While much research has focused on cannabinoids and terpenes, the potential of hemp roots is less explored due to bioproduction challenges. Still, this material is rich in bioactive compounds and demonstrates promising anti-inflammatory, antimicrobial, and antioxidant properties. Biotechnological methods, such as hairy root cultures, enable the efficient production of specialized metabolites while avoiding the issues of outdoors cultures. Despite these benefits, the chemical diversity understanding of hemp hairy roots remains limited. In this study, we conducted an extensive NMR and LC/MS chemical profiling of hemp hairy roots to determine their chemical composition, revealing the presence of cannabisins for the first time. We then investigated the accumulation of cannabisins and triterpenes in both hemp hairy roots and hemp aeroponic roots. Our findings reveal that hairy roots produce 12 times more cannabisins and 6 times more triterpenes than aeroponic roots, respectively, in addition to yielding 3 times more biomass in bioreactors. Preliminary bioassays also suggest antioxidant and antifungal properties. This research underscores the potential of hemp hairy roots as a valuable source of specialized metabolites and calls for further exploration into their bioactive compounds and applications.

Salinity Stress Responses and Adaptation Mechanisms of Zygophyllum propinquum: A Comprehensive Study on Growth, Water Relations, Ion Balance, Photosynthesis, and Antioxidant Defense.

Zygophyllum propinquum (Decne.) is a leaf succulent C4 perennial found in arid saline areas of southern Pakistan and neighboring countries, where it is utilized as herbal medicine. This study investigated how growth, water relations, ion content, chlorophyll fluorescence, and antioxidant system of Z. propinquum change as salinity levels increase (0, 150, 300, 600, and 900 mM NaCl). Salinity increments inhibited total plant fresh weight, whereas dry weight remained constant at moderate salinity and decreased at high salinity. Leaf area, succulence, and relative water content decreased as salinity increased. Similarly, the sap osmotic potential of both roots and shoots declined as NaCl concentrations increased. Except for a transitory increase in roots at 300 mM NaCl, sodium concentrations in roots and shoots increased constitutively to more than five times higher under saline conditions than in non-saline controls. Root potassium increased briefly at 300 mM NaCl but did not respond to NaCl treatments in the leaf. Photosynthetic pigments increased with 300 and 600 mM NaCl compared to non-saline treatments, although carotenoids appeared unaffected by NaCl treatments. Except for very high NaCl concentration (900 mM), salinity showed no significant effect on the maximum efficiency of photosystem II photochemistry (Fv/Fm). Light response curves demonstrated reduced absolute (ETR*) and maximum electron transport rates (ETRmax) for the 600 and 900 mM NaCl treatments. The alpha (α), which indicates the maximum yield of photosynthesis, decreased with increasing NaCl concentrations, reaching its lowest at 900 mM NaCl. Non-photochemical quenching (NPQ) values were significantly higher under 150 and 300 mM NaCl treatments than under non-saline and higher NaCl treatments. Electrolyte leakage, malondialdehyde (MDA), and hydrogen peroxide (H2O2) peaked only at 900 mM NaCl. Superoxide dismutase and glutathione reductase activities and glutathione content in both roots and shoots increased progressively with increasing salinity. Hence, growth reduction under low to moderate (150-600 mM NaCl) salinity appeared to be an induced response, while high (900 mM NaCl) salinity was injurious.

The Untapped Potential of Hairy Root Cultures and Their Multiple Applications.

Plants are rich sources of specialized metabolites, such as alkaloids, terpenes, phenolic acids, flavonoids, coumarins, and volatile oils, which provide various health benefits including anticancer, anti-inflammatory, antiaging, skin-altering, and anti-diabetic properties. However, challenges such as low and inconsistent yields, environment and geographic factors, and species-specific production of some specialized metabolites limit the supply of raw plant material for the food, cosmetic, or pharmaceutical industries. Therefore, biotechnological approaches using plant in vitro systems offer an appealing alternative for the production of biologically active metabolites. Among these, hairy root cultures induced by Rhizobium rhizogenes have firmed up their position as "green cell factories" due to their genotypic and biosynthetic stability. Hairy roots are valuable platforms for producing high-value phytomolecules at a low cost, are amenable to pathway engineering, and can be scaled up in bioreactors, making them attractive for commercialization. This review explores the potential of hairy roots for specialized metabolites biosynthesis focusing on biotechnology tools to enhance their production. Aspects of morphological peculiarities of hairy roots, the diversity of bioreactors design, and process intensification technologies for maximizing biosynthetic capacity, as well as examples of patented plant-derived (green-labeled) products produced through hairy root cultivation at lab and industrial scales, are addressed and discussed.

Salicylic acid priming before cadmium exposure increases wheat growth but does not uniformly reverse cadmium effects on membrane glycerolipids.

Cadmium (Cd) is an abiotic stressor negatively affecting plant growth and reducing crop productivity. The effects of Cd (25 μM) and of pre-soaking seeds with salicylic acid (SA) (500 μM) on morphological, physiological, and glycerolipid changes in two cultivars of wheat (Triticum aestivum L. 'Tosunbey' and 'Cumhuriyet') were explored. Parameters measured were length, fresh and dry biomass, Cd concentration, osmotic potential (ψ), lipid peroxidation, and polar lipid species in roots and leaves, as well as leaf chlorophyll a, carotenoids, and fv/fm. Fresh biomass of roots and leaves and leaf length were strongly depressed by Cd treatment compared to the control, but significantly increased with SA + Cd compared to Cd alone. Cd reduced leaf levels of chlorophyll a, carotenoids, and fv/fm, compared to controls. Treatment with SA + Cd increased pigment levels and fv/fm compared to Cd alone. Cd treatment led to a decrease in DW of total membrane lipids in leaves and depressed levels of monogalactosyldiacylglycerol and phosphatidic acid in leaves and roots of both cultivars. The effects of SA priming and SA + Cd treatment on lipid content and composition were cultivar-specific, suggesting that lipid metabolism may not be a primary target underlying SA remediation of the damaging effects of Cd on wheat growth and development.

Comprehensive Determination of the Phenolic Compound Contents and Antioxidant Potentials of Leaves and Roots of Peucedanum japonicum Harvested from Different Accessions and Growth Periods.

Peucedanum japonicum Thunberg, a medicinal plant, remains understudied despite its potential therapeutic benefits. This study aimed to determine the phytochemical profiles and antioxidant capacities in the extracts of different accessions of P. japonicum by measuring the total polyphenol and flavonoid content of the P. japonicum extracts coupled with DPPH and ABTS+ assays. In addition, phytochemical screening via LC-MS/MS and high-performance liquid chromatography analysis quantified nine compounds wherein chlorogenic acid (CA) was found to be the most abundant in all compounds while hyperoside and peucedanol were the least. Results showed variation in these compounds' content among accessions (2.01-21.31 mg/g CA) and plant parts (0.34-19.57 mg/g CA), with leaves generally showing higher antioxidant activity. The abundance of these compounds These integrated analyses provide insights into the phytochemical composition and antioxidant activity of this understudied plant, contributing to advances in natural product chemistry and potential therapeutic applications.

Potassium alleviates fluoride accumulation and enhances fluoride tolerance in Camellia sinensis

Tea plants [Camellia sinensis (L.) O. Kuntze] are hyperaccumulators of fluoride (F), but excessive F will harm the photosynthesis and metabolic activities of tea plants. Potassium (K) is one of the essential macronutrients of tea plants, which can enhance the photosynthesis of tea plants and their stress resistance. However, there are fewer studies on K in the accumulation of F in tea plants. In this study, we set three K treatment concentrations of 0 mM, 5 mM, 10 mM and finally found that when the tea plants were treated with F, exogenously applied 5 mM K treatment increased chlorophyll content, photosynthesis, and antioxidant enzyme activity, and reduced the extent of cellular damage, where starch particles content was reduced and the interstices of the chloroplast grana layer were more compact. After K treatment, the F content of new shoots decreased, and the flow rate of absorption of K by roots under F treatment was significantly increased through the Non-invasive Micro-test Technology (NMT), and 5 mM K treatment could effectively stabilize the membrane potential of roots. In addition, after K treatment, the relative expression of the F exporter CsFEX in tea plants was significantly upregulated, while the stress resistance gene expression of CsHAKs in tea plants was downregulated. Above all, appropriate K application could effectively alleviate the hyperaccumulation and stress effects of F on tea plants.

Potentials of roots, stems, leaves, flowers, fruits, and seeds extract for the synthesis of silver nanoparticles.

Silver nanoparticles (AgNPs) have gained significant attention in various applications due to their unique properties that differ from bulk or macro-sized counterparts. In the advancement of nanotechnology, a reliable, non-toxic, and eco-friendly green synthesis has widely been developed as an alternative method for the production of AgNPs, overcoming limitations associated with the traditional physical and chemical methods. Green synthesis of AgNPs involves the utilization of biological sources including plant extracts with silver salt as the precursor. The potential of phytochemicals in plant extracts serves as a reducing/capping and stabilizing agent to aid in the bio-reduction of Ag+ ions into a stable nanoform, Ag0. This review provides insights into the potentials of various plant parts like root, stem, leaf, flower, fruit, and seed extracts that have been extensively reported for the synthesis of AgNPs.

Varied root effects on soil detachment with growth time and hydrodynamic characteristics

AbstractPlant roots significantly reduce soil detachment by overland flow. However, there have been few studies on how plant growth time and hydrodynamic characteristics affect the erosion‐reducing potential of plant roots. This study cultivated ryegrass (Lolium perenne L.) and alfalfa (Medicago sativa L.) as representative of plants possessing fibrous roots and taproots, respectively, in silty soil from the Loess Plateau, China. Root‐soil composites were collected monthly from March to September 2021 and subjected to flow scouring in a hydraulic flume at a 15° slope using five different flow discharges (0.05, 0.1, 0.15, 0.2 and 0.3 L s−1). The results of the study indicated that root length density, root surface area density and root mass density exhibited a significant increase during the early growth phase, followed by a slight decrease. All parameters of fibrous roots revealed greater values than those of taproots, except for root mass density. The reduction in soil detachment under varying flow shear stresses showed a significant increase during the early phases of growth, followed by a gradual decline. Notably, fibrous roots demonstrated a greater impact on soil detachment than taproots, and this discrepancy fluctuated over time. Moreover, the contributions of fibrous and taproots to reducing soil detachment decreased from 55.81% to 39.66% and from 38.21% to 20.99% with increasing flow shear stress, respectively. It indicated that the erosion‐reducing potential of plant roots was greater when subjected to low‐flow shear stress compared to high‐flow shear stress. This study can provide a scientific basis for understanding the erosion‐reducing potential of plant roots at different growing stages and under varying hydrodynamic characteristics.

Exploring the medicinal potential of Senna siamea roots: an integrated study of antibacterial and antioxidant activities, phytochemical analysis, ADMET profiling, and molecular docking insights

Nowadays, infectious diseases pose an alarming global threat to human health. The genus Senna is among the most well-known taxonomic categories commonly used in folk medicine to confront these challenges. Motivated by its traditional uses, a comprehensive study was conducted on the roots extract of Senna siamea, aiming to address the in vitro antibacterial and antioxidant efficacy of phytochemicals from the dichloromethane: methanol (1:1) roots extract of the plant, along with in silico computational studies. The separation of compounds was achieved using silica gel column chromatography. Whereas, the antibacterial and antioxidant activities were examined using paper disc diffusion and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays, respectively. Silica gel column chromatography of the dichloromethane: methanol (1:1) roots extract afforded lupeol (1), β-sitosterol (2a) and stigmasterol (2b), chrysophanol (3), betulinic acid (4), and glyceryl-1-hexacosanoate (5). Although these compounds have been previously reported from the plant, proof of their medicinal applications via in vitro and in silico studies is still lacking. Notably, our findings showed remarkable inhibition zones by the extract (18.00 ± 0.00 mm and 17.17 ± 0.24 mm) against E. coli and S. aureus, respectively, at 50 mg/mL compared to ciprofloxacin (23.33 ± 0.47 mm and 22.00 ± 0.00 mm, respectively), showcasing its potential antibacterial efficiency. Considerable inhibition zones were also recorded by chrysophanol (3) against E. coli (16.33 ± 0.24 mm) and S. pyogenes (16.00 ± 0.00 mm) at 2 mg/mL, compared to ciprofloxacin which showed 23.33 ± 0.47 mm and 21.67 ± 0.47 mm, respectively, signifying its potent antibacterial activities. In addition, the crude extract and chrysophanol (3) exhibited substantial IC50 values (1.24 and 1.71 µg/mL, respectively), suggesting their significant antioxidant potential compared to that of ascorbic acid (IC50: 0.53 µg/mL). Chrysophanol (3) fulfilled Lipinski’s rule with no violation and lupeol (1), β-sitosterol (2a), stigmasterol (2b), betulinic acid (4), and glyceryl-1-hexacosanoate (5) displayed one violation each which were in favor of the drug-likeness predictions. All the compounds exhibited no cytotoxicity and except betulinic acid (4), all the compounds also showed no carcinogenicity properties which were consistent with the prediction results of ciprofloxacin. The molecular docking computations revealed that all the compound isolates displayed strong and nearly strong binding affinities against all protein targets, ranging from − 6.6 kcal/mol to -9.2 kcal/mol (lupeol (1) against E. coli DNA gyrase B and topoisomerase II α, respectively). Thus, the present findings suggest the roots of Senna siamea for potential medicinal applications against multi-drug resistant pathogens hence validating its ethno-medicinal uses.

Ground state spectra, decay properties of B and D mesons in a relativistic square root potential

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Xylem plasticity of root, stem, and branch in Cunninghamia lanceolata under drought stress: implications for whole-plant hydraulic integrity.

A better understanding of xylem hydraulic characteristics in trees is critical to elucidate the mechanisms of forest decline and tree mortality from water deficit. As well as temperate forests and forests growing in arid regions, subtropical and tropical forests are also predicted to experience an increased frequency and intensity of climate change-induced drought in the near future. In this study, 1-year-old Cunninghamia lanceolata seedlings (a typical subtropical species in southern China) were selected for a continuous controlled drought pot experiment of 45 days duration. The experimental treatments were non-drought (control), light drought, moderate drought and severe drought stress, which were 80%, 60%, 50%, and 40%, respectively of soil field maximum moisture capacity. The hydraulic conductivity, specific conductivity and water potential of roots, stems, and branches of C. lanceolata all decreased with the prolonging of drought in the different drought intensities. The relative decrease in these hydraulic values were greater in roots than in stems and branches, indicating that roots are more sensitive to drought. Root tracheid diameters normally reduce to ensure security of water transport with prolonged drought, whilst the tracheid diameters of stems and branches expand initially to ensure water transport and then decrease to reduce the risk of embolism with continuing drought duration. The pit membrane diameter of roots, stems and branches generally increased to different extents during the 15-45 days drought duration, which is conducive to enhanced radial water transport ability. The tracheid density and pit density of stems generally decreased during drought stress, which decreased water transport efficiency and increased embolism occurrence. Correlation analysis indicated that anatomical plasticity greatly influenced the hydraulic properties, whilst the relationships varied among different organs. In roots, tracheid diameter decreased and tracheid density increased to enhance water transport security; stems and branches may increase tracheid diameter and pit membrane diameter to increase hydraulic conductivity ability, but may increase the occurrence of xylem embolism. In summary, under drought stress, the xylem anatomical characteristics of C. lanceolata organs were highly plastic to regulate water transport vertically and radially to maintain the trade-off between hydraulic conductivity efficiency and safety.

Novel Insights into the Promoted Accumulation of Nitro-Polycyclic Aromatic Hydrocarbons in the Roots of Legume Plants.

Substituted polycyclic aromatic hydrocarbons (sub-PAHs) are receiving increased attention due to their high toxicity and ubiquitous presence. However, the accumulation behaviors of sub-PAHs in crop roots remain unclear. In this study, the accumulation mechanism of sub-PAHs in crop roots was systematically disclosed by hydroponic experiments from the perspectives of utilization, uptake, and elimination. The obtained results showed an interesting phenomenon that despite not having the strongest hydrophobicity among the five sub-PAHs, nitro-PAHs (including 9-nitroanthracene and 1-nitropyrene) displayed the strongest accumulation potential in the roots of legume plants, including mung bean and soybean. The nitrogen-deficient experiments, inhibitor experiments, and transcriptomics analysis reveal that nitro-PAHs could be utilized by legumes as a nitrogen source, thus being significantly absorbed by active transport, which relies on amino acid transporters driven by H+-ATPase. Molecular docking simulation further demonstrates that the nitro group is a significant determinant of interaction with an amino acid transporter. Moreover, the depuration experiments indicate that the nitro-PAHs may enter the root cells, further slowing their elimination rates and enhancing the accumulation potential in legume roots. Our results shed light on a previously unappreciated mechanism for root accumulation of sub-PAHs, which may affect their biogeochemical processes in soils.

Enhancing biological activities and phenolic content of wild grapevine roots by severe drought stress

Wild grapevines are a rich source of polyphenolic compounds. Various environmental factors can influence these bioactive metabolites, contributing to stress mitigation and adaptation. The present study aimed to investigate the impact of prolonged drought stress on the accumulation of phytochemicals, antioxidants, and antimicrobial activity in the roots of the endangered Tunisian wild grapevine (Vitis vinifera subsp. sylvestris) "Tebaba" genotype. The results revealed that under prolonged drought stress, there was a significant increase in total phenolic compounds, total flavonoid content, and stilbenes, which showed a positive and significant correlation with the total antioxidant activity. Furthermore, the ethanolic extracts demonstrated an enhanced in vitro antibacterial effect against selected human pathogenic bacterial strains, including Staphylococcus aureus, Methicillin-resistant S. aureus, and Enterococcus faecalis. The minimum inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) values, relative to prolonged drought stress, ranged from 125 to 250 µg/mL. The values of the ratio between MBC and the MIC showed that the activity of the root extract was bactericidal in all cases (MBC/MIC ≤ 2). This study highlights the potential of wild grapevine roots enriched with drought-inducible phenolic compounds, as a promising source of antimicrobial agents relevant to human health applications.

The anti-erosion potential of taproots and fibrous roots in alluvial loess of north China: a pot experiment

The anti-erosion potential of taproots and fibrous roots in alluvial loess of north China: a pot experiment

Anti-diabetic potential of Aconitum ferox roots

IntroductionAconitum ferox (A. ferox) is a traditional Chinese plant and recommended by the Chinese medicinal system as an anti-diabetic. According to the Chinese medicinal system, the tubers and roots of A. ferox (Family- Ranunculaceae) are commonly applied for various diseases, such as collapse, syncope, rheumatic fever, painful joints, gastroenteritis, diarrhea, diabetes, edema, bronchial asthma, various tumors, and some endocrinal problems. Till now there is no scientific studies have been published, therefore this plant has been chosen to investigate its anti-diabetic activity. Methods & methodologyWistar rats weighing 150–200 g of either sex were used in the study. Diabetes was induced by alloxan. Diabetes was confirmed after 48 h of a single intraperitoneal injection of alloxan (120 mg/kg) in Wistar rats. Extract of A. ferox (10 and 20 mg/kg) and Glimepiride (175 mg/kg, p.o) orally administered daily for 15 days. On the 15th day body weight was determined and blood was collected to the determine the biochemical parameters like blood glucose level, high-density lipoproteins (HDL), low-density lipoproteins (LDL), triglycerides (TG), total cholesterol (TC) etc. ResultsThe results showed that extract of A. ferox at doses of 10 and 20 mg/kg showed a significant reduction in blood glucose, lipid profile and a significant improvement in body weight when compared to the diabetic control group due to the presence of various primary and secondary metabolites. ConclusionThese results suggest that the root extract of A. ferox possesses an antidiabetic effect on alloxan-induced diabetic rats and this justifies its use in traditional medicine and can be exploited in the management of diabetes but further research must be needed,

Insights into enhancing Centella asiatica organ cell biofactories via hairy root protein profiling.

Recent advancements in plant biotechnology have highlighted the potential of hairy roots as a biotechnological platform, primarily due to their rapid growth and ability to produce specialized metabolites. This study aimed to delve deeper into hairy root development in C. asiatica and explore the optimization of genetic transformation for enhanced bioactive compound production. Previously established hairy root lines of C. asiatica were categorized based on their centelloside production capacity into HIGH, MID, or LOW groups. These lines were then subjected to a meticulous label-free proteomic analysis to identify and quantify proteins. Subsequent multivariate and protein network analyses were conducted to discern proteome differences and commonalities. Additionally, the quantification of rol gene copy numbers was undertaken using qPCR, followed by gene expression measurements. From the proteomic analysis, 213 proteins were identified. Distinct proteome differences, especially between the LOW line and other lines, were observed. Key proteins related to essential processes like photosynthesis and specialized metabolism were identified. Notably, potential biomarkers, such as the Tr-type G domain-containing protein and alcohol dehydrogenase, were found in the HIGH group. The presence of ornithine cyclodeaminase in the hairy roots emerged as a significant biomarker linked with centelloside production capacity lines, indicating successful Rhizobium-mediated genetic transformation. However, qPCR results showed an inconsistency with rol gene expression levels, with the HIGH line displaying notably higher expression, particularly of the rolD gene. The study unveiled the importance of ornithine cyclodeaminase as a traceable biomarker for centelloside production capacity. The strong correlation between this biomarker and the rolD gene emphasizes its potential role in optimizing genetic transformation processes in C. asiatica.

The Effect of Collection Time, IBA and Putrescine Treatments on the Rooting Potential of Foşa Hazelnut Cultivar

Objective: The aim of the research was to determine how collection time, IBA, and putrescine treatments affected the rooting potential of the Foşa hazelnut cultivar (Corylus avellana L.).
 Material and Methods: Cuttings were taken from 1-year-old root suckers of Foşa hazelnut cultivar on 4 different dates (June 15, July 10, August 4 and August 28; in 2021 year). 3 different treatments [Control, IBA (2000 ppm) and IBA+putrescine (2000 ppm+1600 ppm)] were performed to the prepared cuttings. Rooting, callusing, survived cutting ratio, dead cutting ratio, root number, root length, rooting level, number of cutting with buds, number of cutting with shoot, and number of cutting with leaves properties were investigated in cuttings removed from the rooting medium.
 Results: The collection time and treatments had a significant effect on the rooting ability of Foşa hazelnut cuttings. Depending on the collection time and treatments interaction, the callusing ranged from 1.7 (in IBA+putrescine treatment in the June 15) to 96.7% (in IBA treatment in the July 10). Rooting was determined between 0 (in control in the August 3, in control and IBA+putrescine treatments in the August 28) and 26.7% (in IBA treatment in the July 10). The root number was determined from 0 (in control in the August 3, in control and IBA+putrescine treatments in the August 28) to 13.1 (in IBA treatment in the July 10). Root length was measured between 0 (in control in the August 3, in control and IBA+putrescine treatments in the August 28) and 7.4 cm (in IBA treatment in the July 10). The best rooting scale was determined in (2.5) cuttings collected in July 10 and treated with IBA. It was followed by (2.3) cuttings treated IBA collected in June 15.
 Conclusion: It was determined that the rooting ability of IBA-treated semi-hardwood cuttings collected in the early period from Foşa hazelnut cultivar was higher.